BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0544.13
(545 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g27380 glutathione synthetase gsh2 685 0.0
At5g49300 putative protein 33 0.53
At1g75940 ATA27 32 0.90
At5g20320 CAF-like protein 32 1.2
At5g08790 ATAF2 protein 32 1.2
At1g28680 anthranilate N-hydroxycinnamoyl/benzoyltransferase lik... 31 1.5
At5g19500 putative protein 30 2.6
At3g24900 leucine-rich repeat disease resistance protein, putative 30 4.5
At1g55250 unknown protein 30 4.5
At5g53460 NADH-dependent glutamate synthase 29 5.9
At1g72900 unknown protein 29 5.9
At5g40820 AtRAD3 (dbj|BAA92828.1) 29 7.6
At5g15980 unknown protein 28 10.0
At5g10440 cyclin protein - like 28 10.0
At4g22900 unknown protein 28 10.0
At3g45890 unknown protein 28 10.0
At3g07900 putative auxin-independent growth promoter 28 10.0
At1g69550 putative disease resistance protein 28 10.0
>At5g27380 glutathione synthetase gsh2
Length = 539
Score = 685 bits (1767), Expect = 0.0
Identities = 339/540 (62%), Positives = 433/540 (79%), Gaps = 5/540 (0%)
Query: 7 GSGCCFITSGTSHSFSHSTFPSFSSIQLHHQSLSLS-LSFPKHHLKLIMSQH-LSVSSSP 64
GSGC ++ +S + + + F S SS SL L+ SF + K + +Q L S
Sbjct: 2 GSGCSSLSYSSSSTCNATVF-SISSSPSSSSSLKLNPSSFLFQNPKTLRNQSPLRCGRSF 60
Query: 65 IVEEVNDSSSFDYHQIDPELLENVVYDALVYATLNGLLVGDKSVQRSGKVPGVGLVHLPF 124
+E + FD ++D E ++ +VYDALV+++L+GL+VGDKS Q+SG VPGVGL+H P
Sbjct: 61 KME--SQKPIFDLEKLDDEFVQKLVYDALVWSSLHGLVVGDKSYQKSGNVPGVGLMHAPI 118
Query: 125 SLLPPPLPESYWKQACELAPLFNELVDRVSLDGKFLQESLSRTKKADEFTSRLLDIHSKM 184
+LLP PE+YWKQAC + PLFNEL+DRVSLDGKFLQ+SLSRTKK D FTSRLLDIHSKM
Sbjct: 119 ALLPTAFPEAYWKQACNVTPLFNELIDRVSLDGKFLQDSLSRTKKVDVFTSRLLDIHSKM 178
Query: 185 LEINKKEEIRMGLHRSDYMLDEKTKRLLQIEMNTISTSFSGVGCVTTELHRNILSHYGKL 244
LE NKKE+IR+GLHR DYMLDE+T LLQIEMNTIS SF G+ + ++LH+++L YG
Sbjct: 179 LERNKKEDIRLGLHRFDYMLDEETNSLLQIEMNTISCSFPGLSRLVSQLHQSLLRSYGDQ 238
Query: 245 LGLDSIRVPANSATNKFAETLAKAWLEYNNPRAVIMIMVQPEEHNMYDQHMISAVLRDTY 304
+G+DS RVP N++T +FA+ LAKAWLEY+NPRAV+M++VQPEE NMYDQH++S++LR+ +
Sbjct: 239 IGIDSERVPINTSTIQFADALAKAWLEYSNPRAVVMVIVQPEERNMYDQHLLSSILREKH 298
Query: 305 HIPTIRKTLAEVDQEGAVLPDGTLSVDGQAIAIVYFRAGYTPKDYPSESEWRARLLMEQS 364
+I IRKTLAEV++EG+V D TL V GQA+A+VYFR+GYTP D+PSESEW ARLL+E+S
Sbjct: 299 NIVVIRKTLAEVEKEGSVQEDETLIVGGQAVAVVYFRSGYTPNDHPSESEWNARLLIEES 358
Query: 365 SAIKCPPISYHLVGTKKIQQELAKPNVLERFFENKDDVAKLRECFAGLWSLDDSDIVKQA 424
SA+KCP I+YHL G+KKIQQELAKP VLERF +NK+D+AKLR+CFAGLWSLDDS+IVKQA
Sbjct: 359 SAVKCPSIAYHLTGSKKIQQELAKPGVLERFLDNKEDIAKLRKCFAGLWSLDDSEIVKQA 418
Query: 425 IEKPELFVMKPQREGGGNNIYGDDLRETLIKLQKSGSEEDAAYILMQRIFPTSTAAILMR 484
IEKP LFVMKPQREGGGNNIYGDD+RE L++LQK G E +AAYILMQRIFP + L+R
Sbjct: 419 IEKPGLFVMKPQREGGGNNIYGDDVRENLLRLQKEGEEGNAAYILMQRIFPKVSNMFLVR 478
Query: 485 NGCWHKDHVLTEFGIFGTYLRNKDKVIINSESGYMVRTKMSSSDEGGVLPGFGVIDSVYL 544
G +HK ++E G++G YLR+KD+VI+N +SGY++RTK++SSDEGGV GFGV+DS+YL
Sbjct: 479 EGVYHKHQAISELGVYGAYLRSKDEVIVNEQSGYLMRTKIASSDEGGVAAGFGVLDSIYL 538
>At5g49300 putative protein
Length = 139
Score = 32.7 bits (73), Expect = 0.53
Identities = 18/50 (36%), Positives = 26/50 (52%), Gaps = 12/50 (24%)
Query: 433 MKPQREGGGNNIYGDDLRETLIKL------------QKSGSEEDAAYILM 470
+K GGGN +G+ L+++L+ L QK G EE AA +LM
Sbjct: 81 LKKSSSGGGNRKFGESLKQSLMDLGIRKRSTVEKQRQKLGEEEQAAVLLM 130
>At1g75940 ATA27
Length = 535
Score = 32.0 bits (71), Expect = 0.90
Identities = 25/88 (28%), Positives = 41/88 (46%), Gaps = 12/88 (13%)
Query: 388 KPNVLERF--FENKDDVAKLRECFAGLWSLDDSDIVKQAIEKPELFVMKPQREGGGNNIY 445
+P +++F F NK DVAK+ GL SL +K PE+ + + N Y
Sbjct: 378 EPRYVDKFNAFANKPDVAKVEVYAKGLRSL--LKYIKDKYGNPEIMITE--------NGY 427
Query: 446 GDDLRETLIKLQKSGSEEDAAYILMQRI 473
G+DL E L + S++ Y + + +
Sbjct: 428 GEDLGEQDTSLVVALSDQHRTYYIQKHL 455
>At5g20320 CAF-like protein
Length = 1589
Score = 31.6 bits (70), Expect = 1.2
Identities = 14/36 (38%), Positives = 22/36 (60%), Gaps = 1/36 (2%)
Query: 468 ILMQRIFPTSTAAILMRNGCWHKDHVLTEFGIFGTY 503
+L++ IFP S + + + NGCW+ D V +F TY
Sbjct: 909 VLVEDIFPPSGSHLKLANGCWNIDDVKNSL-VFTTY 943
>At5g08790 ATAF2 protein
Length = 283
Score = 31.6 bits (70), Expect = 1.2
Identities = 16/54 (29%), Positives = 28/54 (51%)
Query: 313 LAEVDQEGAVLPDGTLSVDGQAIAIVYFRAGYTPKDYPSESEWRARLLMEQSSA 366
LA VD+ +V L +D + +Y + G K +P++ + R + EQSS+
Sbjct: 131 LANVDRSASVNKKNNLRLDDWVLCRIYNKKGTMEKYFPADEKPRTTTMAEQSSS 184
>At1g28680 anthranilate N-hydroxycinnamoyl/benzoyltransferase like
protein
Length = 451
Score = 31.2 bits (69), Expect = 1.5
Identities = 21/77 (27%), Positives = 39/77 (50%), Gaps = 14/77 (18%)
Query: 126 LLPPPLPESYWKQACELAPLF-----NELVDR-VSLDGKFLQESLSRTKKADEFTSRLLD 179
L+ PPLP+ YW C P++ EL+++ + + +++S S T +DE+ +D
Sbjct: 294 LMNPPLPKGYWGNGC--VPMYAQIKAGELIEQPIWKTAELIKQSKSNT--SDEYVRSFID 349
Query: 180 IHSKMLEINKKEEIRMG 196
E++ K+ I G
Sbjct: 350 FQ----ELHHKDGINAG 362
>At5g19500 putative protein
Length = 533
Score = 30.4 bits (67), Expect = 2.6
Identities = 18/77 (23%), Positives = 30/77 (38%), Gaps = 20/77 (25%)
Query: 426 EKPELFVMKPQREGGGNNIYGDDLRETLIKLQKSGSEEDAAYILMQRIFPTSTAAILMRN 485
+ P F PQR D+ RET + ++ +E+ ++ +R+F + L R
Sbjct: 54 QTPRFFKCLPQR-------VSDERRETQVTTEEEEEDEEEKIVVFERLFSNLNQSTLKRE 106
Query: 486 -------------GCWH 489
GCWH
Sbjct: 107 SEIFRKFVQCNFLGCWH 123
>At3g24900 leucine-rich repeat disease resistance protein, putative
Length = 884
Score = 29.6 bits (65), Expect = 4.5
Identities = 21/60 (35%), Positives = 32/60 (53%), Gaps = 4/60 (6%)
Query: 22 SHSTFPSFSSIQLHHQSLSLSLSFPKHHLKLIMSQHLSVSSSPIVEEVNDSSS-FDYHQI 80
S S S++ LHH L+ SLSF ++ KL + L VS + +N +SS F+ H +
Sbjct: 143 SFSNLSMLSALLLHHNELTGSLSFVRNLRKLTI---LDVSHNHFSGTLNPNSSLFELHNL 199
>At1g55250 unknown protein
Length = 899
Score = 29.6 bits (65), Expect = 4.5
Identities = 45/201 (22%), Positives = 81/201 (39%), Gaps = 32/201 (15%)
Query: 134 SYWKQACELAPLFNELVDRVSLDGKFL---QESLSRTKKADE-----------FTSRLLD 179
SY + + L+N+LVD + L G QE+L+ D+ F RLL
Sbjct: 99 SYDDELISVNQLWNQLVDDLILLGVRAGANQEALNYLDIVDKKRVPPCAADETFLCRLLQ 158
Query: 180 IHSKMLEINKKEEIRMGLHRSDYMLDEKTKRLLQIEMNTISTSFSGVGCVTTELHRNILS 239
+ S L+ +K +E+ + + + T L+ + NTI T + ++ LH
Sbjct: 159 VDS--LDTSKSDEVVRKVEEALALRHSSTMELMGLFENTIDTQKTKAESISQSLH----- 211
Query: 240 HYGKLLGLDSIRVPANSATNKFAETLAKAWLEYNNPRAVI-MIMVQPEEHNMYDQHMISA 298
+ + + +S + E E N R +I + V+ +EH+ Q IS+
Sbjct: 212 ---AVKSTEDATIQLSSINDLMKE-------ESKNLREMIDALHVRHKEHSEQIQAYISS 261
Query: 299 VLRDTYHIPTIRKTLAEVDQE 319
D + ++ L E+ E
Sbjct: 262 HSTDQSELKHLKGQLEEIKAE 282
>At5g53460 NADH-dependent glutamate synthase
Length = 2216
Score = 29.3 bits (64), Expect = 5.9
Identities = 17/49 (34%), Positives = 23/49 (46%), Gaps = 7/49 (14%)
Query: 497 FGIFGTYLRNKDKVIINSESGYMVRTKMSSSDEGGVLPGFGVIDSVYLT 545
FG+ YL N D++ I G + EGG LPG VI + +T
Sbjct: 1085 FGVSSYYLTNADELQIKMAQG-------AKPGEGGELPGHKVIGDIAIT 1126
>At1g72900 unknown protein
Length = 363
Score = 29.3 bits (64), Expect = 5.9
Identities = 29/144 (20%), Positives = 58/144 (40%), Gaps = 10/144 (6%)
Query: 315 EVDQEGAVLPDGTLSVDGQAIAIVYFRAGYTPKDYPSESEWRARLLMEQSSAIKCPPISY 374
E++ + P+ L+++ A+V Y + + + + + +I PI Y
Sbjct: 46 ELENGQMISPELILAIEDSRFAVVVVSVNYAASSWCLDELVKIMDIQKNKGSITVMPIFY 105
Query: 375 -----HL---VG--TKKIQQELAKPNVLERFFENKDDVAKLRECFAGLWSLDDSDIVKQA 424
HL +G ++ ++ A+ LE+ + + +A L + DDS +V
Sbjct: 106 GVNPCHLRRQIGDVAEQFKKHEAREKDLEKVLKWRQALAALADISGDCSGEDDSKLVDVI 165
Query: 425 IEKPELFVMKPQREGGGNNIYGDD 448
+K +M R G N+ G D
Sbjct: 166 ADKISKELMIVTRISNGRNLVGID 189
>At5g40820 AtRAD3 (dbj|BAA92828.1)
Length = 2810
Score = 28.9 bits (63), Expect = 7.6
Identities = 22/75 (29%), Positives = 36/75 (47%), Gaps = 6/75 (8%)
Query: 166 RTKKADEFTSRLLDIHSKMLEINKKEEIRMGLHRSDYMLDEKTKRLLQIEMNTISTSFSG 225
R K D+F R + H +++E + +++M HR + L LL E S+ +
Sbjct: 710 RCSKCDKFIEREDEKHFRIIETPEMVKLKMDHHRDYFNLQSLYFNLLYDE----SSEETQ 765
Query: 226 VGCVTTELHRNILSH 240
+ CV E+ R IL H
Sbjct: 766 LACV--EVIRRILGH 778
>At5g15980 unknown protein
Length = 668
Score = 28.5 bits (62), Expect = 10.0
Identities = 21/67 (31%), Positives = 29/67 (42%), Gaps = 1/67 (1%)
Query: 8 SGCCFITSGTSHSFSHSTFPSFSSIQLHHQSLSLSLSFPKHHLKLIMSQHLSVSSSPIVE 67
S C +TS +S SFS P ++Q Q L L+ S S SS P VE
Sbjct: 23 SPCSQVTSISSRSFSSFIHPGIGALQQSEQLCPLRSPMTSSG-NLVKSVGRSFSSEPAVE 81
Query: 68 EVNDSSS 74
E + + +
Sbjct: 82 EKSSAEA 88
>At5g10440 cyclin protein - like
Length = 317
Score = 28.5 bits (62), Expect = 10.0
Identities = 13/43 (30%), Positives = 23/43 (53%)
Query: 385 ELAKPNVLERFFENKDDVAKLRECFAGLWSLDDSDIVKQAIEK 427
E +PN++ F + K + R F + L+ +IV++ IEK
Sbjct: 3 EFMEPNLVSNFDDEKSNSVDTRSIFQMGFPLESEEIVREMIEK 45
>At4g22900 unknown protein
Length = 343
Score = 28.5 bits (62), Expect = 10.0
Identities = 17/64 (26%), Positives = 29/64 (44%), Gaps = 7/64 (10%)
Query: 190 KEEIRMGLHRSDYMLDEKTKRLLQIEMNT-------ISTSFSGVGCVTTELHRNILSHYG 242
K ++ H D M+ + T R ++ NT + ++FSG+ T +L L YG
Sbjct: 21 KSQLIQSTHLLDLMIRDYTIRNFKLNFNTGVTQKIYLPSNFSGIDIDTVKLRCGSLRRYG 80
Query: 243 KLLG 246
+G
Sbjct: 81 AKIG 84
>At3g45890 unknown protein
Length = 608
Score = 28.5 bits (62), Expect = 10.0
Identities = 17/61 (27%), Positives = 33/61 (53%), Gaps = 6/61 (9%)
Query: 16 GTSHSFSHSTFPSFSSIQLH-----HQSLSLSLSFPKHHLKLIMSQHLSVSSSPIVEEVN 70
GTS S + + F ++I ++ +Q + L P + L+ S++L +P+++EVN
Sbjct: 373 GTSTSLALAAFGVVTTIHMYTNLKSYQCIQLRTLNP-YRASLVFSEYLISGQAPLIKEVN 431
Query: 71 D 71
D
Sbjct: 432 D 432
>At3g07900 putative auxin-independent growth promoter
Length = 579
Score = 28.5 bits (62), Expect = 10.0
Identities = 31/123 (25%), Positives = 52/123 (42%), Gaps = 8/123 (6%)
Query: 347 KDYPSE-SEWRARLLMEQSSAIKCPPISYHLVGTKKIQQELAKPNVLERFFENKDDVAKL 405
KD PS+ + R ++ E A+K P + GTK ++ +K + + DV
Sbjct: 310 KDLPSDLQKLRCKVAFE---ALKFSPRVMEM-GTKLAERMRSKGPYIALHLRMEKDVWVR 365
Query: 406 RECFAGLWSLDDSDIVKQAIEKPELFVMKPQREGGGNNIYG---DDLRETLIKLQKSGSE 462
C +GL S D + + I++PEL K + G + +E L+ G+
Sbjct: 366 TGCLSGLSSKYDEIVNIERIKRPELLTAKSSMTSNERKLAGLCPLNAKEVTRLLRALGAP 425
Query: 463 EDA 465
DA
Sbjct: 426 RDA 428
>At1g69550 putative disease resistance protein
Length = 1398
Score = 28.5 bits (62), Expect = 10.0
Identities = 32/148 (21%), Positives = 60/148 (39%), Gaps = 22/148 (14%)
Query: 10 CCFITSGTSHSFSHSTFPSFSSIQ------LHHQSLSLSLSFPKHHLKLIMSQHLSVSSS 63
C F G H+F + FS++Q + +S L+ P H+L + + + + V +
Sbjct: 507 CFFNDEGIDHTFEDTLRHKFSNVQRGLQVLVQRSLISEDLTQPMHNLLVQLGREI-VRNQ 565
Query: 64 PIVEEVNDSSSFDYHQIDPELLENVVYDAL------VYATLNGLLVGDKSVQRSGKVP-- 115
+ E D +I L + +++ VY +++ L + D+ + +
Sbjct: 566 SVYEPGKRQFLVDGKEICEVLTSHTGSESVIGINFEVYWSMDELNISDRVFEGMSNLQFF 625
Query: 116 -----GVGLVHLPFSL--LPPPLPESYW 136
G +HLP L LPP L +W
Sbjct: 626 RFDENSYGRLHLPQGLNYLPPKLRILHW 653
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,553,635
Number of Sequences: 26719
Number of extensions: 546075
Number of successful extensions: 1616
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 1609
Number of HSP's gapped (non-prelim): 20
length of query: 545
length of database: 11,318,596
effective HSP length: 104
effective length of query: 441
effective length of database: 8,539,820
effective search space: 3766060620
effective search space used: 3766060620
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0544.13