BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0544.11
(200 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g05070 unknown protein 112 2e-25
At4g30990 putative protein 111 3e-25
At2g32580 unknown protein 108 1e-24
At2g24290 unknown protein 105 2e-23
At4g04360 hypothetical protein 91 3e-19
At1g61710 hypothetical protein 32 0.29
At1g20000 hypothetical protein 31 0.38
At2g18330 AAA-type like ATPase 30 0.65
At1g74860 unknown protein 29 1.5
At1g77620 unknown protein 29 1.9
At5g49880 mitotic checkpoint protein-like 28 2.5
At1g12150 hypothetical protein 28 2.5
At3g08880 hypothetical protein 28 3.2
At1g44020 hypothetical protein 28 3.2
At1g79150 hypothetical protein 28 4.2
At1g74160 unknown protein 28 4.2
At1g30860 hypothetical protein 28 4.2
At1g04210 unknown protein 28 4.2
At5g43900 myosin heavy chain MYA2 (pir||S51824) 27 5.5
At5g12000 putative receptor - like kinase 27 5.5
>At1g05070 unknown protein
Length = 184
Score = 112 bits (279), Expect = 2e-25
Identities = 59/144 (40%), Positives = 85/144 (58%), Gaps = 9/144 (6%)
Query: 58 GPLSRWRLKES--ASTESPCHH-PCDCYCSSAESPPDTF------DCGKHDPVMNEEMNK 108
GP W L E+ A + S C PC+C SA + P DC KHDP +NE+ K
Sbjct: 25 GPPLYWHLTEALAAVSASSCPSCPCECSTYSAVTIPKELSNASFADCAKHDPEVNEDTEK 84
Query: 109 DLLTMLSEELNLQRIVTNETLEQTDRLVMNARNASSHYQMETEKCNVAMETCEEARERAE 168
+ +L+EEL L+ + E ++ D ++ A+ +S YQ E +KCN METCEEARE+AE
Sbjct: 85 NYAELLTEELKLREAESLEKHKRADMGLLEAKKVTSSYQKEADKCNSGMETCEEAREKAE 144
Query: 169 AELIEEHRVTALWENRAREYGWKD 192
L E+ ++T+ WE RAR+ GW++
Sbjct: 145 LALAEQKKLTSRWEERARQKGWRE 168
>At4g30990 putative protein
Length = 2895
Score = 111 bits (277), Expect = 3e-25
Identities = 69/186 (37%), Positives = 92/186 (49%), Gaps = 20/186 (10%)
Query: 19 ISEHTASSQSPRSSNGSVSVDVHITCESPSDIVQVIVEFGPLSRWRLKE----SASTESP 74
IS +S PR S + C +V +V GP W+ + S S
Sbjct: 2715 ISAFCSSEAMPRRSGDCMR------CLVIFAVVSALVVCGPALYWKFNKGFVGSTRANSL 2768
Query: 75 CHHPCDCYC----SSAESPP-----DTFDCGKHDPVMNEEMNKDLLTMLSEELNLQRIVT 125
C PC C C S + P DCG DP + +EM K + +L+EEL LQ V
Sbjct: 2769 CP-PCVCDCPPPLSLLQIAPGLANLSITDCGSDDPELKQEMEKQFVDLLTEELKLQEAVA 2827
Query: 126 NETLEQTDRLVMNARNASSHYQMETEKCNVAMETCEEARERAEAELIEEHRVTALWENRA 185
+E + + A+ +S YQ E EKCN A E CE ARERAEA LI+E ++T+LWE RA
Sbjct: 2828 DEHSRHMNVTLAEAKRVASQYQKEAEKCNAATEICESARERAEALLIKERKITSLWEKRA 2887
Query: 186 REYGWK 191
R+ GW+
Sbjct: 2888 RQSGWE 2893
>At2g32580 unknown protein
Length = 183
Score = 108 bits (271), Expect = 1e-24
Identities = 55/143 (38%), Positives = 81/143 (56%), Gaps = 8/143 (5%)
Query: 58 GPLSRWRLKESASTESPCHHPCDCYCSSAE--------SPPDTFDCGKHDPVMNEEMNKD 109
GP W L E+ + + C C CSS S DC K DP +NE+ K+
Sbjct: 25 GPPLYWHLTEALAVSATSCSACVCDCSSLPLLTIPTGLSNGSFTDCAKRDPEVNEDTEKN 84
Query: 110 LLTMLSEELNLQRIVTNETLEQTDRLVMNARNASSHYQMETEKCNVAMETCEEARERAEA 169
+L+EEL + + E ++ D ++ A+ +S YQ E +KCN METCEEARE+AE
Sbjct: 85 YAELLTEELKQREAASMEKHKRVDTGLLEAKKITSSYQKEADKCNSGMETCEEAREKAEK 144
Query: 170 ELIEEHRVTALWENRAREYGWKD 192
L+E+ ++T++WE RAR+ G+KD
Sbjct: 145 ALVEQKKLTSMWEQRARQKGYKD 167
>At2g24290 unknown protein
Length = 173
Score = 105 bits (261), Expect = 2e-23
Identities = 60/156 (38%), Positives = 84/156 (53%), Gaps = 15/156 (9%)
Query: 50 IVQVIVEFGPLSRWRLKE-----SASTESPCHHPCDCY----CSSAESPPDTFD-----C 95
+V ++ GP W+L + + ST S C PC C S + P + C
Sbjct: 17 VVSALLVCGPALYWKLNKGFVGSARSTNSICP-PCVCDFPPPLSLLQIAPGLANLSITGC 75
Query: 96 GKHDPVMNEEMNKDLLTMLSEELNLQRIVTNETLEQTDRLVMNARNASSHYQMETEKCNV 155
G DP + EEM K + +L+EEL LQ V +E + + A+ +S YQ E EKCN
Sbjct: 76 GSDDPELKEEMEKPFVDLLTEELKLQEAVADEHSRHMNVTLAEAKRVASQYQKEAEKCNA 135
Query: 156 AMETCEEARERAEAELIEEHRVTALWENRAREYGWK 191
A E CE ARERA+A L++E ++T LWE RAR+ GW+
Sbjct: 136 ATEICESARERAQALLLKERKITFLWERRARQLGWE 171
>At4g04360 hypothetical protein
Length = 125
Score = 91.3 bits (225), Expect = 3e-19
Identities = 44/99 (44%), Positives = 66/99 (66%), Gaps = 1/99 (1%)
Query: 94 DCGKHDPVMNEEMNKDLLTMLSEELNLQRIVTNETLEQTDRLVMNARNASSHYQMETEKC 153
DC +H+ +EE M++EEL L+ E + DRL+++A+ A+S YQ E +KC
Sbjct: 20 DCMRHEEG-SEESESSFTEMVAEELKLREAQAQEDEWRADRLLLDAKKAASQYQKEADKC 78
Query: 154 NVAMETCEEARERAEAELIEEHRVTALWENRAREYGWKD 192
++ METCE ARE+AEA L E+ R++ +WE RAR+ GWK+
Sbjct: 79 SMGMETCELAREKAEAALDEQRRLSYMWELRARQGGWKE 117
>At1g61710 hypothetical protein
Length = 402
Score = 31.6 bits (70), Expect = 0.29
Identities = 19/67 (28%), Positives = 29/67 (42%), Gaps = 2/67 (2%)
Query: 134 RLVMNARNASSHYQMETEKCN-VAMETCEEARERAEAELIEEHRVTALWENRAREYGWKD 192
+L+ H Q+ KC+ + C RA E ++H +TA+WE RE W +
Sbjct: 286 KLICRVCKTERHNQLNCIKCDFIVCMKCATLPYRARYEH-DKHFLTAIWEEEVREKDWCE 344
Query: 193 KRRTRLR 199
LR
Sbjct: 345 VCERNLR 351
>At1g20000 hypothetical protein
Length = 204
Score = 31.2 bits (69), Expect = 0.38
Identities = 18/62 (29%), Positives = 27/62 (43%)
Query: 81 CYCSSAESPPDTFDCGKHDPVMNEEMNKDLLTMLSEELNLQRIVTNETLEQTDRLVMNAR 140
C+ SS E +C K PV + +KD EE + T +TLE +R V +
Sbjct: 9 CFASSNERVTCNCNCLKDQPVPRMKHSKDPFEAAMEEQEESPVETEQTLEGDERAVKKCK 68
Query: 141 NA 142
+
Sbjct: 69 TS 70
>At2g18330 AAA-type like ATPase
Length = 636
Score = 30.4 bits (67), Expect = 0.65
Identities = 20/94 (21%), Positives = 45/94 (47%), Gaps = 8/94 (8%)
Query: 101 VMNEEMNKDLLTMLSEELNLQRIVTNETLEQTDRLVMNARNA--------SSHYQMETEK 152
+M ++ L + +E+ + + I ++ +E+ +L + RN + + + E E
Sbjct: 99 LMRKQEKTRLAELAAEKEHNEAIQASKDIERQRKLAEDQRNLVQQQAQAKAQNLRYEDEL 158
Query: 153 CNVAMETCEEARERAEAELIEEHRVTALWENRAR 186
M+T EA+ R AEL+ +++ + +AR
Sbjct: 159 ARKRMQTDNEAQRRHNAELVSMQEASSIRKEKAR 192
>At1g74860 unknown protein
Length = 369
Score = 29.3 bits (64), Expect = 1.5
Identities = 19/47 (40%), Positives = 26/47 (54%), Gaps = 1/47 (2%)
Query: 149 ETEKCNVAMETCEEARERAEAELIEEHRVTALWENRAREYGWKDKRR 195
ETE E+CE E + + L EE V AL +NR +Y +K KR+
Sbjct: 306 ETEARKEEKESCENM-EVSASPLCEETPVLALSKNRCIKYTFKRKRK 351
>At1g77620 unknown protein
Length = 1151
Score = 28.9 bits (63), Expect = 1.9
Identities = 16/59 (27%), Positives = 30/59 (50%), Gaps = 1/59 (1%)
Query: 86 AESPPDTFDCGKHDPVMNEEMNKDLL-TMLSEELNLQRIVTNETLEQTDRLVMNARNAS 143
AE P CG D + MN+D++ T S+E+ + R + L+ T ++ + +A+
Sbjct: 821 AELSPRAVSCGHFDGRVEVSMNEDVVQTPPSKEIYIDRFQIFDCLKNTSEIIAESSDAT 879
>At5g49880 mitotic checkpoint protein-like
Length = 726
Score = 28.5 bits (62), Expect = 2.5
Identities = 21/66 (31%), Positives = 32/66 (47%), Gaps = 2/66 (3%)
Query: 127 ETLEQTDRLVMNARNASSHYQMETEKCNVAMETCEEARERAEAELIEEHRVTALWENRAR 186
E L++ + V AR S + ++ E NV + + RERAE+EL + H + E
Sbjct: 283 EELKRYEAEVREARKLKSRH-LDAELLNVNLLEEQSRRERAESELSKFHDLQLSMEKLEN 341
Query: 187 EY-GWK 191
E WK
Sbjct: 342 ELSSWK 347
>At1g12150 hypothetical protein
Length = 548
Score = 28.5 bits (62), Expect = 2.5
Identities = 24/77 (31%), Positives = 35/77 (45%), Gaps = 7/77 (9%)
Query: 101 VMNEEMNKDLLTMLSEELNLQRIVTNETLEQTDRLVMNARNASSHYQMETEKCNVAMETC 160
V+ EEM K E+N +I+TNE E T RL A + + ++ ME
Sbjct: 284 VLREEMKK----AHESEMNTVKIITNELNEATMRL---QEAADDECSLRSLVNSLRMELE 336
Query: 161 EEARERAEAELIEEHRV 177
+ RER E + E R+
Sbjct: 337 DLRREREELQQKEAERL 353
>At3g08880 hypothetical protein
Length = 107
Score = 28.1 bits (61), Expect = 3.2
Identities = 21/97 (21%), Positives = 41/97 (41%), Gaps = 9/97 (9%)
Query: 108 KDLLTMLSEELNLQRI-----VTNETLEQTDRLVMNARNASSHYQMETEKCNVAMETCEE 162
+DL++ + +NL + + +++ EQ L + Q E C + C++
Sbjct: 11 EDLMSYGDDLINLLNVKNGFDIISQSSEQFKALNFACHEDFNQIQGSIEDCKTKLYACKK 70
Query: 163 ARERAEAELIEEHRVTALW----ENRAREYGWKDKRR 195
E A +++ E + L E RE+ KD+ R
Sbjct: 71 KTEEAYSDIAAEDEIERLQKELDEEMEREFKLKDELR 107
>At1g44020 hypothetical protein
Length = 654
Score = 28.1 bits (61), Expect = 3.2
Identities = 19/63 (30%), Positives = 27/63 (42%)
Query: 68 SASTESPCHHPCDCYCSSAESPPDTFDCGKHDPVMNEEMNKDLLTMLSEELNLQRIVTNE 127
S T+S D S D+ D EE ++L+++LSE +L TNE
Sbjct: 133 STDTKSKPEPEVDSELMFVHSSMDSDSDSDSDLDSEEEEEEELISLLSEMYSLYAFTTNE 192
Query: 128 TLE 130
LE
Sbjct: 193 ELE 195
>At1g79150 hypothetical protein
Length = 495
Score = 27.7 bits (60), Expect = 4.2
Identities = 18/69 (26%), Positives = 35/69 (50%), Gaps = 6/69 (8%)
Query: 129 LEQTDRLVMNARNASSHYQMETEKCNVAMETCEEARERAEAELIEEHRVTALWENRAREY 188
++ D L + + HY+ E++K +A E + AE +++E+ V L +++ RE
Sbjct: 89 VDPVDVLPVKTLDGKLHYRTESKKSKLA----EAETDEAEKDVLEDEHV--LNKSQRREK 142
Query: 189 GWKDKRRTR 197
K KR +
Sbjct: 143 AKKSKREAK 151
>At1g74160 unknown protein
Length = 1028
Score = 27.7 bits (60), Expect = 4.2
Identities = 20/77 (25%), Positives = 41/77 (52%), Gaps = 8/77 (10%)
Query: 103 NEEMNKDLLTMLSEELNLQRIVTNETLEQTDRLVMNA-----RNASSHYQMETEKCNVAM 157
NE++N+ L+ L E+ ++++ + +E T +M + + A S Q+ E C+
Sbjct: 887 NEKLNRKLVFDLVNEILVEKLAS---VEATTNPLMKSYAKVTKKAVSAQQLLKELCSAIE 943
Query: 158 ETCEEARERAEAELIEE 174
++A +R+E L+EE
Sbjct: 944 TQQKQATKRSENFLLEE 960
>At1g30860 hypothetical protein
Length = 739
Score = 27.7 bits (60), Expect = 4.2
Identities = 19/74 (25%), Positives = 32/74 (42%), Gaps = 2/74 (2%)
Query: 101 VMNEEMNKDLLTMLSEELNLQRIVTNETLEQTDRLVMNARNASSHYQMETEKCNVAMETC 160
VMN KD + L E + + + E+ D L+M+ +H ++K + E
Sbjct: 512 VMNTRSEKDDICRLLERRTVTDFLDSGLREKIDNLMMS--RVQTHSDKHSKKWELQQEEE 569
Query: 161 EEARERAEAELIEE 174
EE + E+ EE
Sbjct: 570 EEVNFEIDEEIKEE 583
>At1g04210 unknown protein
Length = 1112
Score = 27.7 bits (60), Expect = 4.2
Identities = 19/60 (31%), Positives = 31/60 (51%), Gaps = 4/60 (6%)
Query: 82 YCSSAESPPDTF-DCGKHDPVMNEEMNKDLLTMLSEELNLQRIVTNETLEQTDRLVMNAR 140
+CS+ +S PD F D G+ P M + +L + S E+ L +E L D + ++AR
Sbjct: 512 FCSTEDSLPDGFFDAGRDRPFMTLSKYEKVLPLDSREVILLDRAKDEVL---DAITLSAR 568
>At5g43900 myosin heavy chain MYA2 (pir||S51824)
Length = 1505
Score = 27.3 bits (59), Expect = 5.5
Identities = 25/101 (24%), Positives = 48/101 (46%), Gaps = 8/101 (7%)
Query: 85 SAESPPDTFDCGKHDPVMNEEM-------NKDLLTMLSEELNLQRIVTNETLEQTDRLVM 137
+A+ +T K PV+++E+ N+ L +M+S L ++ T + L++T ++
Sbjct: 949 AAKKIAETAPIIKEIPVVDQELMDKITNENEKLKSMVSS-LEMKIGETEKKLQETTKISQ 1007
Query: 138 NARNASSHYQMETEKCNVAMETCEEARERAEAELIEEHRVT 178
+ N + + + K AM+ EE EAE H+ T
Sbjct: 1008 DRLNQALEAESKLVKLKTAMQRLEEKILDMEAEKKIMHQQT 1048
>At5g12000 putative receptor - like kinase
Length = 703
Score = 27.3 bits (59), Expect = 5.5
Identities = 17/49 (34%), Positives = 23/49 (46%), Gaps = 4/49 (8%)
Query: 126 NETLEQTDRLVMNARNASSHY----QMETEKCNVAMETCEEARERAEAE 170
N+ + R ARNA +ME KC A+E E+A+ AE E
Sbjct: 327 NQWKMEEARRFEEARNAEEAALAVAEMEKAKCRAALEAAEKAQRMAELE 375
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.315 0.128 0.381
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,365,624
Number of Sequences: 26719
Number of extensions: 165476
Number of successful extensions: 652
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 16
Number of HSP's that attempted gapping in prelim test: 638
Number of HSP's gapped (non-prelim): 27
length of query: 200
length of database: 11,318,596
effective HSP length: 94
effective length of query: 106
effective length of database: 8,807,010
effective search space: 933543060
effective search space used: 933543060
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0544.11