BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0520.11
(117 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g46770 unknown protein 54 1e-08
At5g67410 putative protein 32 0.066
At3g48060 putative protein 29 0.43
At3g48050 putative protein 29 0.43
At1g69570 H-protein promoter binding factor-2b, putative 29 0.43
At1g65760 hypothetical protein 29 0.43
At2g36930 unknown protein 28 0.73
At3g05750 hypothetical protein 28 0.96
At2g39640 putative beta-1,3-glucanase, predicted GPI-anchored pr... 27 1.3
At5g24700 unknown protein 27 1.6
At1g71930 NAM-like protein 27 1.6
At1g50780 hypothetical protein 27 1.6
At5g28620 putative protein 27 2.1
At5g16240 stearoyl-acyl carrier protein desaturase 27 2.1
At3g49490 putative protein 27 2.1
At2g15650 putative retroelement pol polyprotein 27 2.1
At5g60890 Myb transcription factor homolog (ATR1) 26 2.8
At2g42710 unknown protein 26 2.8
At1g64280 ranscription factor inhibitor I kappa B-like protein 26 2.8
At1g64080 unknown protein 26 2.8
>At5g46770 unknown protein
Length = 133
Score = 53.9 bits (128), Expect = 1e-08
Identities = 48/131 (36%), Positives = 61/131 (45%), Gaps = 33/131 (25%)
Query: 1 MSLNCLTCSQNLQRTDS------FGEFFTEKEYKEVCKKV-------DRNWSGNLIASSS 47
MSLNCL+C Q L RTDS G E V K RNWSGNL
Sbjct: 1 MSLNCLSC-QALPRTDSNKDVDLSGPGPPRVEINNVLGKTCCVNPIGGRNWSGNL----- 54
Query: 48 SSSSTSQCDLPKGQGGNVAKIKAEHRRVH----------STGNIPYPGSSQPKLVRSSGM 97
S + P G ++A + +++H S N+P QPKLVRS+G+
Sbjct: 55 SPRIYEKIGRP---GSSLAHKMKKVKKIHHVRLSGPVGSSPSNVP-TRPEQPKLVRSTGV 110
Query: 98 RRDWSFENLAE 108
RR+WSFENL +
Sbjct: 111 RRNWSFENLRD 121
>At5g67410 putative protein
Length = 287
Score = 31.6 bits (70), Expect = 0.066
Identities = 19/44 (43%), Positives = 26/44 (58%), Gaps = 2/44 (4%)
Query: 73 RRVHSTGNIPYPGSSQPKLVRSSGMRRDWSFENLAENQDQSVSC 116
RR++ GNI S ++V SSG R+ WS EN+ E DQ + C
Sbjct: 122 RRLNKDGNISKGKSLVTEVVSSSG-RQQWSMENV-EEVDQLIPC 163
>At3g48060 putative protein
Length = 1611
Score = 28.9 bits (63), Expect = 0.43
Identities = 20/64 (31%), Positives = 30/64 (46%), Gaps = 9/64 (14%)
Query: 39 SGNLIASSSSSSSTSQCDLPKGQGGNVAKIKAEHRRVHSTGNIPYPGSSQPKLVRSSGMR 98
+GNL + SST+ L K GG+ + HR+ N + GSS R +G+
Sbjct: 534 TGNLCGKEDARSSTAGSTLKKCSGGS-----SRHRK----SNNVFQGSSSSASPRGAGLS 584
Query: 99 RDWS 102
R +S
Sbjct: 585 RSFS 588
>At3g48050 putative protein
Length = 1613
Score = 28.9 bits (63), Expect = 0.43
Identities = 20/64 (31%), Positives = 30/64 (46%), Gaps = 9/64 (14%)
Query: 39 SGNLIASSSSSSSTSQCDLPKGQGGNVAKIKAEHRRVHSTGNIPYPGSSQPKLVRSSGMR 98
+GNL + SST+ L K GG+ + HR+ N + GSS R +G+
Sbjct: 534 TGNLCGKEDARSSTAGSTLKKCSGGS-----SRHRK----SNNVFQGSSSSASPRGAGLS 584
Query: 99 RDWS 102
R +S
Sbjct: 585 RSFS 588
>At1g69570 H-protein promoter binding factor-2b, putative
Length = 399
Score = 28.9 bits (63), Expect = 0.43
Identities = 24/71 (33%), Positives = 34/71 (47%), Gaps = 8/71 (11%)
Query: 35 DRNWSGNLIASSSSSSSTSQCDLPKGQGGNVAKIKAEHRRVHSTGNIPYPGSSQPKLV-- 92
D N SG +++ SS SS ++ K Q G+ AKI A+ S N Y G P+++
Sbjct: 202 DNNNSGTILSFGSSESSVTETG--KHQSGDTAKISADS---VSQENKSYQGFLPPQVMLP 256
Query: 93 -RSSGMRRDWS 102
SS WS
Sbjct: 257 NNSSPWPYQWS 267
>At1g65760 hypothetical protein
Length = 362
Score = 28.9 bits (63), Expect = 0.43
Identities = 13/38 (34%), Positives = 21/38 (55%)
Query: 21 FFTEKEYKEVCKKVDRNWSGNLIASSSSSSSTSQCDLP 58
++TE + +VCK D N S ++ S SS S+ + P
Sbjct: 322 YYTENTWPKVCKLEDGNGSSIIVTMSESSKSSFEMFYP 359
>At2g36930 unknown protein
Length = 198
Score = 28.1 bits (61), Expect = 0.73
Identities = 11/33 (33%), Positives = 17/33 (51%)
Query: 4 NCLTCSQNLQRTDSFGEFFTEKEYKEVCKKVDR 36
NCL C +N F K++K+ KK++R
Sbjct: 139 NCLLCHRNFSNASVMDYHFKTKKHKKRVKKIER 171
>At3g05750 hypothetical protein
Length = 798
Score = 27.7 bits (60), Expect = 0.96
Identities = 24/83 (28%), Positives = 39/83 (46%), Gaps = 14/83 (16%)
Query: 33 KVDRNWSGNLIASSSSSSSTSQCDLPKGQGGNVAKIKAEHRRVHSTGNIPYPGSSQPK-- 90
++ +N + N + SS S+ST D GQG + A R+ +IP P + +P+
Sbjct: 63 EIGKNSTYNPRSDSSCSTSTPTSD--DGQGSKAPSVVA---RLMGLESIPVPNALEPRRN 117
Query: 91 ------LVRSSGMRRDW-SFENL 106
+RSS W ++ENL
Sbjct: 118 PDFDPYFLRSSRKASTWDAYENL 140
>At2g39640 putative beta-1,3-glucanase, predicted GPI-anchored
protein
Length = 549
Score = 27.3 bits (59), Expect = 1.3
Identities = 18/42 (42%), Positives = 26/42 (61%), Gaps = 3/42 (7%)
Query: 31 CKKVDRNWSGNLIASSSSSSSTSQCDLP--KGQGGNVAKIKA 70
C V ++SG I +S++ S TS C +P +G GGN AK K+
Sbjct: 417 CVDVVCDFSGTGIVTSTNPS-TSTCPIPIGEGGGGNGAKSKS 457
>At5g24700 unknown protein
Length = 129
Score = 26.9 bits (58), Expect = 1.6
Identities = 13/38 (34%), Positives = 21/38 (55%)
Query: 44 ASSSSSSSTSQCDLPKGQGGNVAKIKAEHRRVHSTGNI 81
+S S S+ST C + KG+ G+V + E ++ T I
Sbjct: 72 SSGSFSASTQMCPVMKGKFGSVGAVTLEKGKLDMTQKI 109
>At1g71930 NAM-like protein
Length = 324
Score = 26.9 bits (58), Expect = 1.6
Identities = 13/42 (30%), Positives = 22/42 (51%), Gaps = 1/42 (2%)
Query: 75 VHSTGNIPYPGSSQPKLVRSSGMRRDWSFENLAENQDQSVSC 116
+H GNI P P L S G +D + E+ + +++S +C
Sbjct: 224 MHQYGNIELPQLDSPSLSPSLGTNKDQN-ESFEQEEEKSFNC 264
>At1g50780 hypothetical protein
Length = 323
Score = 26.9 bits (58), Expect = 1.6
Identities = 19/54 (35%), Positives = 26/54 (47%)
Query: 24 EKEYKEVCKKVDRNWSGNLIASSSSSSSTSQCDLPKGQGGNVAKIKAEHRRVHS 77
+KE K D+N N +SSSSSSS+SQ GQ + + +H S
Sbjct: 146 KKEKSYSVSKSDQNPKNNGGSSSSSSSSSSQIASDFGQNPSTSSPFFDHHSYSS 199
>At5g28620 putative protein
Length = 130
Score = 26.6 bits (57), Expect = 2.1
Identities = 18/74 (24%), Positives = 28/74 (37%), Gaps = 10/74 (13%)
Query: 23 TEKEYKEVCKKVDRNWSG----------NLIASSSSSSSTSQCDLPKGQGGNVAKIKAEH 72
T E K K+ + W+ N +A S + T +C L KG + +
Sbjct: 11 TSPETKPPAKEGEEKWNRATKVGLLKRENCVARSDKAGGTHRCSLDKGSSFALMVHRGLV 70
Query: 73 RRVHSTGNIPYPGS 86
+H+ NI P S
Sbjct: 71 YEIHNEANILVPRS 84
>At5g16240 stearoyl-acyl carrier protein desaturase
Length = 394
Score = 26.6 bits (57), Expect = 2.1
Identities = 16/67 (23%), Positives = 33/67 (48%), Gaps = 9/67 (13%)
Query: 22 FTEKEYKEVCKKVDRNWSGNLIASSSSSSSTSQ---CDLPKGQGGNVAKI--KAEHRRVH 76
+T K+Y ++ + + W+ ++ SS + +Q C LP + K+ +A+ R
Sbjct: 322 YTAKDYADILQHLVERWNVEKLSDLSSEGNRAQDYLCGLP----ARIRKLEERAQGRTKE 377
Query: 77 STGNIPY 83
+ NIP+
Sbjct: 378 AAKNIPF 384
>At3g49490 putative protein
Length = 953
Score = 26.6 bits (57), Expect = 2.1
Identities = 15/54 (27%), Positives = 26/54 (47%), Gaps = 2/54 (3%)
Query: 36 RNWSGNLIASSSSSSSTSQCDLPKGQGGNVAKIKAEHRRVHSTGNI--PYPGSS 87
R+WS ++ S S+ T + G + +KA++ S GN P+ GS+
Sbjct: 380 RSWSHFMVTSEGPSAPTMFSMGSESGGPSAPTMKADNENAQSAGNYKPPFEGST 433
>At2g15650 putative retroelement pol polyprotein
Length = 1347
Score = 26.6 bits (57), Expect = 2.1
Identities = 12/35 (34%), Positives = 18/35 (51%)
Query: 15 TDSFGEFFTEKEYKEVCKKVDRNWSGNLIASSSSS 49
T +FG FT KE + D +W G+L S++
Sbjct: 1176 TSNFGVLFTSKETPRLVGYSDSDWGGSLEDKKSTT 1210
>At5g60890 Myb transcription factor homolog (ATR1)
Length = 295
Score = 26.2 bits (56), Expect = 2.8
Identities = 11/27 (40%), Positives = 14/27 (51%)
Query: 9 SQNLQRTDSFGEFFTEKEYKEVCKKVD 35
S N TD F EFF +E ++ VD
Sbjct: 218 SDNCSFTDEFNEFFNNEEISDIYTTVD 244
>At2g42710 unknown protein
Length = 415
Score = 26.2 bits (56), Expect = 2.8
Identities = 19/52 (36%), Positives = 25/52 (47%)
Query: 44 ASSSSSSSTSQCDLPKGQGGNVAKIKAEHRRVHSTGNIPYPGSSQPKLVRSS 95
+SSSSSSS+S D N ++ KA S P S+PK V S+
Sbjct: 34 SSSSSSSSSSSSDSNPSPDSNESRKKAVTIEPVSYAAKPKDQKSEPKNVEST 85
>At1g64280 ranscription factor inhibitor I kappa B-like protein
Length = 593
Score = 26.2 bits (56), Expect = 2.8
Identities = 19/74 (25%), Positives = 33/74 (43%), Gaps = 2/74 (2%)
Query: 3 LNCLTCSQNLQRTDSFGEFFTEKE--YKEVCKKVDRNWSGNLIASSSSSSSTSQCDLPKG 60
+NC +Q D E +K+ Y E+ + + + +S + + +SS + S K
Sbjct: 519 MNCEDLTQLACGEDDTAEKRLQKKQRYMEIQETLKKAFSEDNLELGNSSLTDSTSSTSKS 578
Query: 61 QGGNVAKIKAEHRR 74
GG + K HRR
Sbjct: 579 TGGKRSNRKLSHRR 592
>At1g64080 unknown protein
Length = 411
Score = 26.2 bits (56), Expect = 2.8
Identities = 15/32 (46%), Positives = 19/32 (58%)
Query: 44 ASSSSSSSTSQCDLPKGQGGNVAKIKAEHRRV 75
+SSSSSSS+S K Q GN + + E R V
Sbjct: 234 SSSSSSSSSSSSSSLKKQNGNESVVSDEKRFV 265
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.311 0.125 0.374
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,910,363
Number of Sequences: 26719
Number of extensions: 116453
Number of successful extensions: 416
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 24
Number of HSP's successfully gapped in prelim test: 20
Number of HSP's that attempted gapping in prelim test: 380
Number of HSP's gapped (non-prelim): 49
length of query: 117
length of database: 11,318,596
effective HSP length: 93
effective length of query: 24
effective length of database: 8,833,729
effective search space: 212009496
effective search space used: 212009496
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 52 (24.6 bits)
Lotus: description of TM0520.11