BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0387.5
(124 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g19190 unknown protein 81 1e-16
At3g06070 unknown protein 71 2e-13
At5g61300 putative protein 33 0.027
At2g19200 hypothetical protein 28 0.88
At5g02640 unknown protein 28 1.2
At5g52870 unknown protein (At5g52870) 27 2.0
At5g09840 putative protein 27 2.6
At2g48080 unknown protein 27 2.6
At5g17510 glutamine-rich protein 27 3.4
At1g36550 hypothetical protein 27 3.4
At3g03460 hypothetical protein 26 4.4
At2g13570 putative CCAAT-box binding trancription factor 26 4.4
At2g06890 putative retroelement integrase 26 4.4
At1g06970 hypothetical protein 26 4.4
At1g04440 casein kinase I like protein 26 4.4
At3g55690 unknown protein 26 5.7
At2g39870 unknown protein 26 5.7
At1g14650 splicing factor, putative 25 7.5
At5g11990 predicted GPI-anchored protein 25 9.8
At1g68550 putative AP2 domain transcription factor 25 9.8
>At5g19190 unknown protein
Length = 154
Score = 81.3 bits (199), Expect = 1e-16
Identities = 51/104 (49%), Positives = 72/104 (69%), Gaps = 11/104 (10%)
Query: 1 MEVAVELEDDLFFADLSKQLSLLIMDEDD--DPLA-SCAVHPQSFSGAIHPPPQSA-ILY 56
M + +EL+DD+FFAD+SKQ+SLLIMDED+ +P++ S + SF G Q+A +Y
Sbjct: 1 MAMELELDDDVFFADISKQISLLIMDEDEHLNPVSLSSSSSSLSFQGLFRGGYQTAPYMY 60
Query: 57 EQVLRRQSKGTGVFIPQSTQPRRK----QRKGRTSSYAKYQKQS 96
+Q QSKGTGVFIP+S+QPRR+ Q++GR SS+ Q+ S
Sbjct: 61 QQ---EQSKGTGVFIPKSSQPRRRPHHHQKQGRYSSFNAKQQHS 101
>At3g06070 unknown protein
Length = 151
Score = 70.9 bits (172), Expect = 2e-13
Identities = 40/87 (45%), Positives = 55/87 (62%), Gaps = 7/87 (8%)
Query: 1 MEVAVELEDDLFFADLSKQLSLLIMDEDDD---PLASCAVHPQSFSGAIHPPPQSAILYE 57
M + +EL+DD+FFAD+SKQL+LLI DED+ L+S F G ++Y
Sbjct: 1 MAMELELDDDVFFADISKQLNLLITDEDEQNPISLSSSVSFQGLFRGNYQTSATPYMMYN 60
Query: 58 QVLR----RQSKGTGVFIPQSTQPRRK 80
+ + R+SKGTGVFIP+S+QPRRK
Sbjct: 61 EQINYNVIRESKGTGVFIPRSSQPRRK 87
>At5g61300 putative protein
Length = 557
Score = 33.5 bits (75), Expect = 0.027
Identities = 32/115 (27%), Positives = 52/115 (44%), Gaps = 13/115 (11%)
Query: 10 DLFFADLSKQLSLLIMDEDDDPLASCAVHPQSFSGAIHPPPQSAILYEQVLRRQSKGTGV 69
DL + LS + + I E L S ++H + S P +S +L L S +
Sbjct: 239 DLNMSPLSPEEEVPIESEQPRELVSASLHGKHGS----EPSRSLVLALPCLETIS----L 290
Query: 70 FIPQSTQPRRKQRKGRTSSYAKYQKQSQDTRMISHVPIKNPFKETNDRAYIDPSA 124
F + +QPR+K ++G AKY K++ T+ + + F D +YI SA
Sbjct: 291 FHTRCSQPRKKAKRG-----AKYVKKNPRTKSRKGSNLDSNFGVNEDASYIPESA 340
>At2g19200 hypothetical protein
Length = 246
Score = 28.5 bits (62), Expect = 0.88
Identities = 10/29 (34%), Positives = 19/29 (65%)
Query: 94 KQSQDTRMISHVPIKNPFKETNDRAYIDP 122
K++ +I +P++NPF++ DR Y+ P
Sbjct: 75 KRTMLVSLIPELPLENPFQDATDRFYVLP 103
>At5g02640 unknown protein
Length = 161
Score = 28.1 bits (61), Expect = 1.2
Identities = 15/45 (33%), Positives = 24/45 (53%), Gaps = 3/45 (6%)
Query: 61 RRQSKGTGVFIPQSTQPRRKQRKGRTSSYAKYQKQSQDTRMISHV 105
R+ + G+ I +ST+PRR+ K R S + KQ Q +I +
Sbjct: 103 RKSKEENGIIIVESTKPRRRGEKRRVS---RKNKQEQACSVILRI 144
>At5g52870 unknown protein (At5g52870)
Length = 326
Score = 27.3 bits (59), Expect = 2.0
Identities = 25/103 (24%), Positives = 40/103 (38%), Gaps = 7/103 (6%)
Query: 1 MEVAVELEDDLFFADLSKQLSLLIMDEDDDPLASCAVHPQSFSGAIHPPPQSAILYEQVL 60
+ V ++D+ K S+ + DD + +V + FS I P LY +
Sbjct: 136 LNVRFRVDDETTTTSFRKTASIARSQQTDDTMFDDSVSKRFFS-LIKP------LYTKST 188
Query: 61 RRQSKGTGVFIPQSTQPRRKQRKGRTSSYAKYQKQSQDTRMIS 103
++QS T S R KQR S ++Q +R S
Sbjct: 189 KKQSSSTITSPTSSPATREKQRSNIPSGMRSVRRQLGKSRSAS 231
>At5g09840 putative protein
Length = 924
Score = 26.9 bits (58), Expect = 2.6
Identities = 17/64 (26%), Positives = 29/64 (44%), Gaps = 2/64 (3%)
Query: 27 EDDDPLASCAVHPQSFSGAIHPPPQSAILYEQVLRRQSKGTGV--FIPQSTQPRRKQRKG 84
EDD LA + P S + HP + +YE L + +G P+ + + ++G
Sbjct: 815 EDDSDLAFAELGPVSDTTTTHPTTKKLPVYEPSLSEDEEDSGSERDNPEKKKQQMMSKEG 874
Query: 85 RTSS 88
+ SS
Sbjct: 875 KESS 878
>At2g48080 unknown protein
Length = 433
Score = 26.9 bits (58), Expect = 2.6
Identities = 15/49 (30%), Positives = 21/49 (42%), Gaps = 8/49 (16%)
Query: 43 SGAIHPPP--------QSAILYEQVLRRQSKGTGVFIPQSTQPRRKQRK 83
SG + PPP ++ +R GTGVF+P + RK K
Sbjct: 354 SGKVQPPPTLWRPGTPSPLVMLAPAPKRLDAGTGVFLPWTPPVSRKPAK 402
>At5g17510 glutamine-rich protein
Length = 369
Score = 26.6 bits (57), Expect = 3.4
Identities = 19/81 (23%), Positives = 33/81 (40%), Gaps = 10/81 (12%)
Query: 46 IHPPPQSAILYE----------QVLRRQSKGTGVFIPQSTQPRRKQRKGRTSSYAKYQKQ 95
I+PPPQ Q ++Q+ + + Q Q +++Q+ R ++ + Q
Sbjct: 76 INPPPQQNPTPNPNLGQQTPNFQQQQQQNVSSQQMMQQQQQQQQQQKLMRPLNHIELQCA 135
Query: 96 SQDTRMISHVPIKNPFKETND 116
QD + H K PF D
Sbjct: 136 YQDAWRVCHPNFKQPFSSLED 156
>At1g36550 hypothetical protein
Length = 353
Score = 26.6 bits (57), Expect = 3.4
Identities = 14/37 (37%), Positives = 21/37 (55%)
Query: 53 AILYEQVLRRQSKGTGVFIPQSTQPRRKQRKGRTSSY 89
AIL+EQ ++R+S + + QR+ RTSSY
Sbjct: 203 AILFEQQVKRKSSSRSSYGSGTVAKPTYQREERTSSY 239
>At3g03460 hypothetical protein
Length = 354
Score = 26.2 bits (56), Expect = 4.4
Identities = 19/74 (25%), Positives = 34/74 (45%), Gaps = 5/74 (6%)
Query: 46 IHPPP---QSAILYEQVLRRQSKGTGVFIPQSTQPRRKQRKGRTSSYAKYQKQSQDTRMI 102
++P P Q A ++Q ++Q + + Q Q +++Q+ R S+ + Q QD +
Sbjct: 76 VNPNPNLIQQANKFQQ--QQQQQMMMMMQQQQLQQQQQQKLMRPSNQLEIQFAYQDAWRV 133
Query: 103 SHVPIKNPFKETND 116
H K PF D
Sbjct: 134 CHPDFKRPFASLED 147
>At2g13570 putative CCAAT-box binding trancription factor
Length = 215
Score = 26.2 bits (56), Expect = 4.4
Identities = 12/37 (32%), Positives = 22/37 (59%)
Query: 62 RQSKGTGVFIPQSTQPRRKQRKGRTSSYAKYQKQSQD 98
R ++G V P+ Q R++Q++ + ++ YQ Q QD
Sbjct: 124 RDTEGEKVNSPKQQQQRQQQQQIQQQNHHNYQFQEQD 160
>At2g06890 putative retroelement integrase
Length = 1215
Score = 26.2 bits (56), Expect = 4.4
Identities = 14/37 (37%), Positives = 21/37 (55%)
Query: 53 AILYEQVLRRQSKGTGVFIPQSTQPRRKQRKGRTSSY 89
AIL+EQ ++R+S + + QR+ RTSSY
Sbjct: 86 AILFEQQVKRKSSSRSSYGSGTIAKPTYQREERTSSY 122
>At1g06970 hypothetical protein
Length = 829
Score = 26.2 bits (56), Expect = 4.4
Identities = 11/40 (27%), Positives = 18/40 (44%)
Query: 77 PRRKQRKGRTSSYAKYQKQSQDTRMISHVPIKNPFKETND 116
P Q + + ++++Q+Q T M H PF ND
Sbjct: 528 PNTVQSTHIVNGFQRFEQQNQGTLMAQHFTAAAPFSSIND 567
>At1g04440 casein kinase I like protein
Length = 468
Score = 26.2 bits (56), Expect = 4.4
Identities = 11/27 (40%), Positives = 16/27 (58%)
Query: 55 LYEQVLRRQSKGTGVFIPQSTQPRRKQ 81
++E RR GTGV QS++PR +
Sbjct: 342 VFEAYTRRNGSGTGVQADQSSRPRTSE 368
>At3g55690 unknown protein
Length = 293
Score = 25.8 bits (55), Expect = 5.7
Identities = 18/59 (30%), Positives = 28/59 (46%), Gaps = 12/59 (20%)
Query: 38 HPQSFSGAI----HPPPQSAILYEQV---LRRQSKGTGVFIPQ-----STQPRRKQRKG 84
+PQS G + H PP + + ++ S GTGVF+P+ S P++K G
Sbjct: 180 YPQSQMGIVPNAWHIPPSPVRAFYTLPTAVKSPSTGTGVFLPRKYSNPSDSPKKKSGDG 238
>At2g39870 unknown protein
Length = 330
Score = 25.8 bits (55), Expect = 5.7
Identities = 13/25 (52%), Positives = 16/25 (64%), Gaps = 3/25 (12%)
Query: 49 PPQSAILYEQVLRRQSKGTGVFIPQ 73
PPQ A L+R S GTGVF+P+
Sbjct: 231 PPQQAAA---PLKRPSAGTGVFLPR 252
>At1g14650 splicing factor, putative
Length = 785
Score = 25.4 bits (54), Expect = 7.5
Identities = 13/35 (37%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Query: 31 PLASCAVHPQSFSGAIHPPPQSAILYEQVLRRQSK 65
P A+ A H ++ G IHPPP + E+ + SK
Sbjct: 48 PPAAVATHTRTI-GIIHPPPDIRTIVEKTAQFVSK 81
>At5g11990 predicted GPI-anchored protein
Length = 181
Score = 25.0 bits (53), Expect = 9.8
Identities = 16/60 (26%), Positives = 24/60 (39%), Gaps = 5/60 (8%)
Query: 31 PLASCAVHPQSFSGAIHPPPQSAILYEQV-----LRRQSKGTGVFIPQSTQPRRKQRKGR 85
P S ++ P IHPPP +E L KG+ +P S + Q +G+
Sbjct: 78 PPLSQSLSPPPLITVIHPPPPRFYYFESTPPPPPLSPDGKGSPPSVPSSPPSPKGQSQGQ 137
>At1g68550 putative AP2 domain transcription factor
Length = 324
Score = 25.0 bits (53), Expect = 9.8
Identities = 13/44 (29%), Positives = 20/44 (44%)
Query: 58 QVLRRQSKGTGVFIPQSTQPRRKQRKGRTSSYAKYQKQSQDTRM 101
++ +R + T +PQ TQP RK R YA S + +
Sbjct: 16 EIKKRAKRNTLSSLPQETQPLRKVRIIVNDPYATDDSSSDEEEL 59
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.316 0.131 0.371
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,898,530
Number of Sequences: 26719
Number of extensions: 113638
Number of successful extensions: 295
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 13
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 280
Number of HSP's gapped (non-prelim): 20
length of query: 124
length of database: 11,318,596
effective HSP length: 87
effective length of query: 37
effective length of database: 8,994,043
effective search space: 332779591
effective search space used: 332779591
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 53 (25.0 bits)
Lotus: description of TM0387.5