BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0383.6
(493 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g17930 unknown protein 35 0.12
At5g17940 putative protein 35 0.12
At2g23480 hypothetical protein 33 0.47
At2g14360 pseudogene 33 0.47
At5g63980 3'(2'),5'-bisphosphate nucleotidase 32 0.80
At3g60870 putative protein 32 1.0
At1g79280 hypothetical protein 32 1.0
At3g22520 unknown protein 31 1.4
At3g62890 putative protein 31 1.8
At2g27880 Argonaute (AGO1)-like protein 31 1.8
At5g47370 homeobox-leucine zipper protein-like 30 2.3
At3g04260 hypothetical protein 30 2.3
At2g29210 proline-rich protein like 30 3.0
At1g80930 unknown protein 30 3.0
At4g39040 unknown protein 30 4.0
At3g18610 unknown protein 30 4.0
At1g76970 unknown protein 30 4.0
At1g25400 unknown protein 30 4.0
At4g12050 putative DNA-binding protein 29 5.2
At2g24550 unknown protein 29 5.2
>At5g17930 unknown protein
Length = 707
Score = 34.7 bits (78), Expect = 0.12
Identities = 17/63 (26%), Positives = 33/63 (51%)
Query: 53 RNHKAMSEVSTSSSEHEGDEPVDEGQRDDGVVVTPRVTEKQTSESIDDMLEETVQHLREA 112
++H+ + E++ EHE E DE ++ V R K+ +S+D+ LE + ++ +
Sbjct: 170 QDHEDVDELANEDLEHEESEFSDEESEEEPVGKRDRKRHKKKKKSVDEELESDLMNITDD 229
Query: 113 GYS 115
G S
Sbjct: 230 GES 232
>At5g17940 putative protein
Length = 427
Score = 34.7 bits (78), Expect = 0.12
Identities = 17/63 (26%), Positives = 33/63 (51%)
Query: 53 RNHKAMSEVSTSSSEHEGDEPVDEGQRDDGVVVTPRVTEKQTSESIDDMLEETVQHLREA 112
++H+ + E++ EHE E DE ++ V R K+ +S+D+ LE + ++ +
Sbjct: 190 QDHEDVDELANEDLEHEESEFSDEESEEEPVGKRDRKRHKKKKKSVDEELESDLMNITDD 249
Query: 113 GYS 115
G S
Sbjct: 250 GES 252
>At2g23480 hypothetical protein
Length = 705
Score = 32.7 bits (73), Expect = 0.47
Identities = 36/141 (25%), Positives = 66/141 (46%), Gaps = 21/141 (14%)
Query: 48 SGMSSRN--HKAMSEVSTSSSEHEGDEPVDEGQR-DDGVVVTPRVTEKQTSESIDDMLEE 104
SG SS+ + SE + ++ E PV+E +R DDG+ V E+ ++ D +E+
Sbjct: 152 SGSSSQTLGSDSDSEETETNKEVACANPVEEAERQDDGLAVIEEEEERSSASDEDVNVEK 211
Query: 105 TVQHLREAGYSLEEIKDLAVLKDWNFENFAGYRLEEGIEE---VECVAAVNECKELAVVN 161
+V+ + G E +D+ V ++E+ +EE E +A V+E E +V
Sbjct: 212 SVE---DEGDEDERDEDVIV------------QVEKPVEERTIDEDIANVDERNEDVIVQ 256
Query: 162 VANGLSEYMLCNQIEAVNVHD 182
V + E + I V++ +
Sbjct: 257 VEKPVEERTIAEDIANVDMEE 277
>At2g14360 pseudogene
Length = 221
Score = 32.7 bits (73), Expect = 0.47
Identities = 39/166 (23%), Positives = 72/166 (42%), Gaps = 31/166 (18%)
Query: 44 VYLASGMSSRNHKAMSEVSTSSSEHEGD-------EPVDEGQRDDGVVVTPRVTEKQTSE 96
VY ++ SS + SE TS E+EGD EP + + + + PRV ++ + +
Sbjct: 12 VYDSTEKSSSGEVSTSEPVTSEIENEGDAADLVPTEPAETAEIEK-AMEEPRVGDEASPK 70
Query: 97 SIDDMLEETVQHLREAGYSLEEIKDLAVLKDWNFENFAGYRLEEGIEEVECVAAVNECKE 156
++ E+ + G +L+ + D + + ++ G LEE EE+ VN +
Sbjct: 71 GTAEIEED------QTGTTLKPLIDAVIQQFEEAKDGNGEELEE--EEM-----VNTIPK 117
Query: 157 LAVVNVANGLSEYMLCNQIEAVNVHDDLINLKGTQKKETEETNFEE 202
V NV N+ + DD +++K T++ E E+
Sbjct: 118 EGVENV----------NRQDTCEAEDDEVSVKETEEDEASNDGLED 153
>At5g63980 3'(2'),5'-bisphosphate nucleotidase
Length = 353
Score = 32.0 bits (71), Expect = 0.80
Identities = 39/159 (24%), Positives = 65/159 (40%), Gaps = 19/159 (11%)
Query: 210 EGGQVGKVW-FDNSD--KAFLVGQQWKVNCGVRAQSVLNVGSVSCNDNPLRW----GMNL 262
EGG G+ W D D K FL G Q+ V G+ + + +G ++C + PL N
Sbjct: 123 EGGPNGRHWVLDPIDGTKGFLRGDQYAVALGLLEEGKVVLGVLACPNLPLASIAGNNKNK 182
Query: 263 RWPNNTWVMPSLLTGAGSSRRLIYDQNYGKTLQTGGVATPTQSQVIRFGGLLRKIFTGPR 322
+ + G+G+ +L+ ++ +Q V P ++ + F G
Sbjct: 183 SSSDEIGCLFFATIGSGTYMQLLDSKSSPVKVQVSSVENPEEASFF-------ESFEGAH 235
Query: 323 VSLHQIKKIARSTKGVAASPLNTLS----GWDKKSDGSI 357
SLH + + GV A P+ S G + DG+I
Sbjct: 236 -SLHDLSSSIANKLGVKAPPVRIDSQAKYGALSRGDGAI 273
>At3g60870 putative protein
Length = 265
Score = 31.6 bits (70), Expect = 1.0
Identities = 18/51 (35%), Positives = 25/51 (48%), Gaps = 9/51 (17%)
Query: 397 KQRRGRKKGG---------LGALRGRKTEAKPKPKRGRPRGSKAKPKQQVI 438
+Q+RGR++ G L + K + RGRP GSK KPK +I
Sbjct: 27 RQKRGREEEGVEPNNIGEDLATFPSGEENIKKRRPRGRPAGSKNKPKAPII 77
>At1g79280 hypothetical protein
Length = 2111
Score = 31.6 bits (70), Expect = 1.0
Identities = 16/63 (25%), Positives = 28/63 (44%)
Query: 60 EVSTSSSEHEGDEPVDEGQRDDGVVVTPRVTEKQTSESIDDMLEETVQHLREAGYSLEEI 119
E + ++E DEPV+E + + T + QT E + L + E L+ +
Sbjct: 1855 EYVEAQQDNEADEPVEESPTETETIPTEEESRDQTEEENQEPLTDMESDKEEGELDLDTL 1914
Query: 120 KDL 122
+DL
Sbjct: 1915 EDL 1917
>At3g22520 unknown protein
Length = 600
Score = 31.2 bits (69), Expect = 1.4
Identities = 21/76 (27%), Positives = 34/76 (44%), Gaps = 3/76 (3%)
Query: 402 RKKGGLGALRGRKTEAKPKPKRGRPRGSKAKPKQQVIDLPISDDDFEWEDASARARHTWK 461
+KKG G ++ KPKP R R + + + +DL +++ E A A T
Sbjct: 267 KKKGATTPATGPQSSTKPKPSRQSGRRTSIQQRGGAVDLNFLNEE---ESEPATAPKTSN 323
Query: 462 VAKQLGVVYKGREDEI 477
K+ GV Y+ + I
Sbjct: 324 KRKKRGVNYEEEDVSI 339
>At3g62890 putative protein
Length = 558
Score = 30.8 bits (68), Expect = 1.8
Identities = 19/49 (38%), Positives = 27/49 (54%)
Query: 92 KQTSESIDDMLEETVQHLREAGYSLEEIKDLAVLKDWNFENFAGYRLEE 140
+Q SE I ML+E +Q LREAGY + + L L + + E Y E+
Sbjct: 449 QQESERIYAMLDEIMQRLREAGYVTDTKEVLLDLNEKDKEIALSYHSEK 497
>At2g27880 Argonaute (AGO1)-like protein
Length = 997
Score = 30.8 bits (68), Expect = 1.8
Identities = 35/124 (28%), Positives = 58/124 (46%), Gaps = 18/124 (14%)
Query: 7 ARILVDSSIPITTSREITVLLDGEIVKVMVEPDHSAGVYLASGMSSR-NHKAMSEVSTSS 65
A+I SS+PI RE+ L+ + K +V+ Y A + R ++A+ + T S
Sbjct: 406 AKISGISSLPI---RELRFTLEDKSEKTVVQ-------YFAEKYNYRVKYQALPAIQTGS 455
Query: 66 SEHEGDEPVDEGQRDDGVVVTPRVTEKQTSESIDDMLEETVQHLREAGYSLEEIKDLAVL 125
P++ Q D+G T R+ EKQ + +L+ T Q + S IK+L V
Sbjct: 456 DTRPVYLPMELCQIDEGQRYTKRLNEKQ----VTALLKATCQRPPDRENS---IKNLVVK 508
Query: 126 KDWN 129
++N
Sbjct: 509 NNYN 512
>At5g47370 homeobox-leucine zipper protein-like
Length = 283
Score = 30.4 bits (67), Expect = 2.3
Identities = 22/64 (34%), Positives = 31/64 (48%), Gaps = 1/64 (1%)
Query: 48 SGMSSRNHKAMSEVSTSSSEHEGDEPVDEGQRDDGVVVTP-RVTEKQTSESIDDMLEETV 106
+G+SS N S +S SE EG G DD +TP R + TS+ +D E +
Sbjct: 70 TGVSSPNSTISSTISGKRSEREGISGTGVGSGDDHDEITPDRGYSRGTSDEEEDGGETSR 129
Query: 107 QHLR 110
+ LR
Sbjct: 130 KKLR 133
>At3g04260 hypothetical protein
Length = 913
Score = 30.4 bits (67), Expect = 2.3
Identities = 28/113 (24%), Positives = 48/113 (41%), Gaps = 5/113 (4%)
Query: 56 KAMSEVSTSSSEHEGDEPVDEGQRDDGVVVTPRVTEKQTSESIDDMLEETVQHLREAGYS 115
+A +E E EGDE DE + ++ VVV + + + + + +HL+ G
Sbjct: 652 EADNEEDDDEEEQEGDEDDDENEEEE-VVVPETENRAEGEDLVKNKAADAKKHLQMIGVQ 710
Query: 116 LEEIKDLAVLKDWNFENFAGYRLEEGIEE----VECVAAVNECKELAVVNVAN 164
L + D A + + LE+ +E E A E +E V +VA+
Sbjct: 711 LLKESDEANRTKKRGKRASRMTLEDDADEDWFPEEPFEAFKEMRERKVFDVAD 763
>At2g29210 proline-rich protein like
Length = 878
Score = 30.0 bits (66), Expect = 3.0
Identities = 20/61 (32%), Positives = 34/61 (54%), Gaps = 3/61 (4%)
Query: 378 EEIDTEGFGLSDTEILDK--VKQRRGRKKGGLGALRGRKTEAKPKPKRGRPRGSKAKPKQ 435
EE+ ++ G SD+++ D+ K+RR +K + R+ E + + KR RG K K K+
Sbjct: 772 EEVSSDDNGSSDSDVDDRKEAKRRRKEEKKTRKEEKKRRREERHR-KREERRGGKEKHKK 830
Query: 436 Q 436
Q
Sbjct: 831 Q 831
>At1g80930 unknown protein
Length = 900
Score = 30.0 bits (66), Expect = 3.0
Identities = 35/150 (23%), Positives = 58/150 (38%), Gaps = 18/150 (12%)
Query: 353 SDGSIENNLVVGVQACIGHTNVFAVEEIDTEGFGLSDTEILD-----------KVKQRRG 401
SDG E + G + + EE D +G E+ D KV +++G
Sbjct: 112 SDGDGERRVNKGRNTDRVRADTSSDEEDDLKGNKKEPMEVDDDYGRRGRRRSPKVMEKQG 171
Query: 402 RKKGGLGALRGRKTEAKPKPKRGRPRGSKAKPKQQVIDLPISDDDFEWEDASARARHTWK 461
R++ G+ + + R R RG + + +++ D SDDD E E S R
Sbjct: 172 RERSHRGSRVIADKPSDEEDDRQRSRGGRRESQRKRRDHRASDDDEEGEIRSERRGKEKN 231
Query: 462 VAKQLGVVYKGREDEIVQRLEKQLRENHPN 491
G++ + R E+ L + H N
Sbjct: 232 DRGSEGLLKRDRR-------ERDLTDGHEN 254
>At4g39040 unknown protein
Length = 296
Score = 29.6 bits (65), Expect = 4.0
Identities = 25/101 (24%), Positives = 50/101 (48%), Gaps = 15/101 (14%)
Query: 38 PDHSAGVYLASGMSS-----RNHKAMSEVSTSSSEHEGDEPVDEGQRDDGVVVTPR-VTE 91
P HSA + + S S + + S+ E+E ++ +E ++D GVVV+ R + +
Sbjct: 62 PCHSASLVVKSFSSIDEPDLEEDEESEDEDFSAEEYEYEDEEEEDEQDSGVVVSERGIED 121
Query: 92 KQTSESIDDM--LEETVQHLRE-------AGYSLEEIKDLA 123
+ SE + ++ EE ++ ++ S++E K+LA
Sbjct: 122 SEASEEVSEIGDKEEKTENTKKKKSRGSALKLSIKEKKELA 162
>At3g18610 unknown protein
Length = 636
Score = 29.6 bits (65), Expect = 4.0
Identities = 18/58 (31%), Positives = 33/58 (56%), Gaps = 3/58 (5%)
Query: 50 MSSRNHKAMSEVSTSSSEHEGDEPVDEGQRDDGVVVTPRVTEKQTSESIDDMLEETVQ 107
M + K+++EV T +S +P+ +G+RD + +VT+KQ E ID + +E +
Sbjct: 1 MGKSSKKSVTEVETPASM---TKPLKKGKRDAEEDLDMQVTKKQKKELIDVVQKEKAE 55
>At1g76970 unknown protein
Length = 446
Score = 29.6 bits (65), Expect = 4.0
Identities = 33/134 (24%), Positives = 57/134 (41%), Gaps = 23/134 (17%)
Query: 333 RSTKGVAASPLNTLSGWDKKSDGSIENNLVVG-VQACIGH-------TNVFAVEEIDTEG 384
+S +G ++ L D + S++ ++V V+ C + N EE+ +G
Sbjct: 182 QSAEGSVDVLMDMLGAHDPGNPESLKEEVIVDLVEQCRTYQRRVMTLVNTTTDEELLCQG 241
Query: 385 FGLSDTEILDKVKQRRGRKKGGLGALRGRKTEAKPKPKRGRPRGSKAKPKQQVIDLPISD 444
L+D L V QR +G++ P GR ++A P Q++D+ D
Sbjct: 242 LALNDN--LQHVLQRHD-DIANVGSV----------PSNGR--NTRAPPPVQIVDINHDD 286
Query: 445 DDFEWEDASARARH 458
+D E +D AR H
Sbjct: 287 EDDESDDEFARLAH 300
>At1g25400 unknown protein
Length = 288
Score = 29.6 bits (65), Expect = 4.0
Identities = 29/108 (26%), Positives = 47/108 (42%), Gaps = 4/108 (3%)
Query: 32 VKVMVEPDHSAGVYLASGMSSRNHKAMSEVSTSSSEHEGDEPVDEGQRDDGVVVTPRVTE 91
+K +V + V L S N+ + S+ S SS + ++ D+ + DD V RV
Sbjct: 65 IKTLVFRLRNVNVSLPSQTLLCNYDSDSDWSFSSDSSDEEKDEDDNKEDDSVNGDSRVQR 124
Query: 92 KQTSESIDDM-LEETVQHLREAGYSLEEIKDL---AVLKDWNFENFAG 135
DD + +V LR S ++ DL V+K W+ +F G
Sbjct: 125 FGYYHDDDDKGISGSVPWLRRCSGSFGDLLDLGSSGVVKLWDNLDFNG 172
>At4g12050 putative DNA-binding protein
Length = 339
Score = 29.3 bits (64), Expect = 5.2
Identities = 16/35 (45%), Positives = 19/35 (53%), Gaps = 2/35 (5%)
Query: 404 KGGLGALRGRKTEAKPKPKRGRPRGSKAKPKQQVI 438
+GG G G E + RGRP GSK KPK +I
Sbjct: 104 EGGSGG--GGSGEQMTRRPRGRPAGSKNKPKAPII 136
>At2g24550 unknown protein
Length = 208
Score = 29.3 bits (64), Expect = 5.2
Identities = 18/53 (33%), Positives = 26/53 (48%)
Query: 50 MSSRNHKAMSEVSTSSSEHEGDEPVDEGQRDDGVVVTPRVTEKQTSESIDDML 102
MS+ N+K+ E S SSS +E + +D V R T S S++D L
Sbjct: 53 MSNNNNKSPEESSDSSSSIGESSENEEEEEEDDAVSCQRGTLDSFSSSLEDSL 105
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.313 0.132 0.380
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,418,053
Number of Sequences: 26719
Number of extensions: 512720
Number of successful extensions: 1383
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 23
Number of HSP's that attempted gapping in prelim test: 1366
Number of HSP's gapped (non-prelim): 38
length of query: 493
length of database: 11,318,596
effective HSP length: 103
effective length of query: 390
effective length of database: 8,566,539
effective search space: 3340950210
effective search space used: 3340950210
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0383.6