BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0383.4
(221 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g73350 unknown protein 256 7e-69
At2g46790 hypothetical protein 35 0.024
At1g41820 hypothetical protein 34 0.053
At2g02790 unknown protein 33 0.12
At1g24560 putative myosin heavy chain 32 0.35
At5g40450 unknown protein 31 0.45
At1g48920 unknown protein 31 0.45
At2g43650 unknown protein 31 0.59
At1g16630 Hypothetical protei 31 0.59
At2g12090 pseudogene; similar to MURA transposase of maize Muta... 30 0.77
At1g16270 putative Ser/Thr protein kinase 30 0.77
At4g21550 VP1 like protein 30 1.0
At3g63510 unknown protein 30 1.0
At3g02810 putative protein kinase 30 1.0
At4g30160 putative villin 30 1.3
At5g16730 putative protein 29 1.7
At3g44040 putative protein 29 1.7
At1g77180 unknown protein 29 2.2
At5g65900 ATP-dependent RNA helicase-like 28 2.9
At5g47680 unknown protein 28 2.9
>At1g73350 unknown protein
Length = 208
Score = 256 bits (654), Expect = 7e-69
Identities = 143/221 (64%), Positives = 165/221 (73%), Gaps = 13/221 (5%)
Query: 1 MAGREVREYTNLSDPKDKKWGKGKDKIDDEDVTFQRMVAKMQEVAGERGGYLHGRGALDS 60
MAGREV+EYTNLSDPKDKK GKGK IDDEDVTFQRMVAKMQEVAGERGGYLHGRG DS
Sbjct: 1 MAGREVKEYTNLSDPKDKKSGKGK--IDDEDVTFQRMVAKMQEVAGERGGYLHGRG--DS 56
Query: 61 DDLLYLKEQMEAEEDAERLLRRTEKRAFAAFKRAASLVDSSPASVPLPLRVEPKPKSGIR 120
DDLLYLKEQMEAEEDAERLLRRTEKRAFAAFK+AA+ DSSPAS+PLPLRVEPKPKSGIR
Sbjct: 57 DDLLYLKEQMEAEEDAERLLRRTEKRAFAAFKKAATQADSSPASIPLPLRVEPKPKSGIR 116
Query: 121 QQDLLKKVVEIKPKRPRSESKQSTSASSDVSVTNRKPDHGRLKETGQSLSGVKKVEEQSL 180
QQDLL+KVVE+KPKRP+ + S S S V ++R P ++ Q + +++
Sbjct: 117 QQDLLRKVVEVKPKRPKISTPSSPSLSPPVR-SDRGPTEAKVHRDKQKEEAMPVLKKPGA 175
Query: 181 SGSTKVEDLSSGSHDADAKPKINNSGGGLLGLAYASSDDDE 221
+ S+G N+ GLLGLAY SSD+++
Sbjct: 176 DRNDGKATESNGQG--------QNAPKGLLGLAYDSSDEED 208
>At2g46790 hypothetical protein
Length = 454
Score = 35.4 bits (80), Expect = 0.024
Identities = 33/141 (23%), Positives = 61/141 (42%), Gaps = 15/141 (10%)
Query: 73 EEDAERLLRRTEKRAFAAFKRAASLVDSSPASVPLPLRVEPKPKSGIRQQDLLKKVVEIK 132
+E + L ++ AF+ F+ + S + A V L +PK+ + L+KV +
Sbjct: 284 DESKHQKLSLSDASAFSRFEESKS---AEKAVVALEESTSGEPKTPTESHEKLRKVTSDQ 340
Query: 133 PKRPRSESKQSTSASS--------DVSVTNRKPDHGRLKETGQSLSGVKKVEEQSLSGST 184
S ++++ +SS +VTN+K D E+ + + K+VE +GS
Sbjct: 341 GSATTSSNQENIGSSSVSFRNQVLQSTVTNQKQDSPIPVESNREKAASKEVE----AGSQ 396
Query: 185 KVEDLSSGSHDADAKPKINNS 205
+ +G + KPK S
Sbjct: 397 STNEGIAGQSSSTEKPKEEES 417
>At1g41820 hypothetical protein
Length = 401
Score = 34.3 bits (77), Expect = 0.053
Identities = 34/139 (24%), Positives = 62/139 (44%), Gaps = 18/139 (12%)
Query: 68 EQMEAEEDAERLLRR------TEKRAFAAFKRAASLVDSSPASVPLPLRVEPKPKS---- 117
EQ+ AEE A RR T K + +++D+ + + + KP +
Sbjct: 14 EQLVAEEQALAEPRRQMTRMVTSKSSSGVSVNDQAIIDAITLQMEKIIDAKHKPINDQLK 73
Query: 118 GIRQQDLLKKVVEIKPKRPRSESKQSTSASSDVS--VTNRKPDHGRLKETGQSLSGVKKV 175
GI Q + + E KP RP+ + + + D S + DH R ++ Q + VK+V
Sbjct: 74 GIEQYEQISHQKEAKPNRPQEKHNWRSRPTKDESNCYYYIRDDHRRFEQARQQPNDVKEV 133
Query: 176 E------EQSLSGSTKVED 188
+ ++SLS + +++D
Sbjct: 134 KTMKQNRKESLSSNEQLKD 152
>At2g02790 unknown protein
Length = 650
Score = 33.1 bits (74), Expect = 0.12
Identities = 31/124 (25%), Positives = 53/124 (42%), Gaps = 9/124 (7%)
Query: 67 KEQMEAEEDAERLLRRTEKRAFAAFKRAASLVDSSPASVPLPLRVEPKPKSGIRQQDLL- 125
K +A E + L+R ++ A S S A KPK +R+ L
Sbjct: 279 KRSFQAVEAEKGKLKRGVRKPTGVSTTANSSTSRSTAD-------NEKPKRTVRKASTLG 331
Query: 126 KKVVEIKPKRPRSESKQSTSASSDVSVTNRKPDHGRLKETGQSLS-GVKKVEEQSLSGST 184
K++ +I+ + + S++STSA + S K + R+ SLS G+ K +S
Sbjct: 332 KELSKIENDKSKQSSRKSTSAIKEGSSVEVKDEKPRISHKKASLSNGIGKATRKSAEKKK 391
Query: 185 KVED 188
++ D
Sbjct: 392 EIAD 395
>At1g24560 putative myosin heavy chain
Length = 673
Score = 31.6 bits (70), Expect = 0.35
Identities = 31/121 (25%), Positives = 55/121 (44%), Gaps = 14/121 (11%)
Query: 29 DEDVTFQRMVAKMQEVAGERGGYLHGRGALDSD---DLLYLKEQMEAEEDAERLLRRTEK 85
+E++T ++ A+ + AG GA++ D L LKE++E + A ++ +K
Sbjct: 530 EEEITRWKVAAEQEAAAG---------GAVEQDFTSQLYVLKEELEEAKQA--IIESEKK 578
Query: 86 RAFAAFKRAASLVDSSPASVPLPLRVEPKPKSGIRQQDLLKKVVEIKPKRPRSESKQSTS 145
F AA++ A L L K R Q+L +KV E++ R + S ++
Sbjct: 579 LKFKEETAAAAMGARDAAERSLRLADNRATKLRERIQELNRKVEELETHRDMNTSNRARY 638
Query: 146 A 146
A
Sbjct: 639 A 639
>At5g40450 unknown protein
Length = 2910
Score = 31.2 bits (69), Expect = 0.45
Identities = 33/184 (17%), Positives = 68/184 (36%), Gaps = 17/184 (9%)
Query: 8 EYTNLSDPKDKKWGKGKDKIDDEDVTFQRMVAKMQEVAGERGG---YLHGRGALDSDDLL 64
E + + +P D++ KG D ++ + F + + + + G++ G + L L
Sbjct: 2237 EVSMIKEPADQEEKKGDDVVESNEKDFVSDILEAKRLHGDKSGEAEKIKEESGLAGKSLP 2296
Query: 65 YLKEQMEAEEDAERLLRRTEKRAFAAFKRAASLVDSSPASVPLPLRVEPKPKSGIRQQDL 124
+ ++ E E ++ + R L+ SSP S +Q+
Sbjct: 2297 IEEINLQEEHKEEVKVQEETREIAQVLPREEILISSSPLSA--------------EEQEH 2342
Query: 125 LKKVVEIKPKRPRSESKQSTSASSDVSVTNRKPDHGRLKETGQSLSGVKKVEEQSLSGST 184
+ + + + P+ + STS + V + K KE K E+Q +
Sbjct: 2343 VISDEKQEEREPQQDFNGSTSEKISLQVEHLKDFETSKKEQKDETHETVKEEDQIVDIKD 2402
Query: 185 KVED 188
K +D
Sbjct: 2403 KKKD 2406
>At1g48920 unknown protein
Length = 557
Score = 31.2 bits (69), Expect = 0.45
Identities = 42/167 (25%), Positives = 72/167 (42%), Gaps = 37/167 (22%)
Query: 16 KDKKWGKGKDKIDDEDVTFQRMVAKMQEVAGERGGYLHGRGALDSDDLLYLKEQMEAEED 75
K KK +D ED ++ AK+ + A + + SDD +++ED
Sbjct: 151 KAKKESSSEDDSSSEDEPAKKPAAKIAKPAA--------KDSSSSDD--------DSDED 194
Query: 76 AERLLRRTEKRAFAAFKRAASLVDSSPASVPLPLRVEPKPKSGIRQQDLLKKVVEIKPKR 135
+E T+K A AA K AAS DSS +D ++ + KP +
Sbjct: 195 SEDEKPATKKAAPAAAK-AASSSDSS-------------------DEDSDEESEDEKPAQ 234
Query: 136 PRSESKQSTSASSDVSVTNRKPDHGRLKETGQSLSG-VKKVEEQSLS 181
++++K S +SSD S + + + +ET + S V+ V+ + S
Sbjct: 235 KKADTKASKKSSSDESSESEEDESEDEEETPKKKSSDVEMVDAEKSS 281
>At2g43650 unknown protein
Length = 654
Score = 30.8 bits (68), Expect = 0.59
Identities = 45/189 (23%), Positives = 77/189 (39%), Gaps = 33/189 (17%)
Query: 19 KWGKGKDKIDDEDVTFQRMVAKMQEVAGERGGYLHGRGALDSDDLLYLKEQMEAEEDAER 78
K+G G D++ D+D + + G R G H +D D L E ++AEE E
Sbjct: 99 KFGDGDDEMADDDKDKEE---DKRSTWGGRSGLYHSGDNVDFDILSSDDEDIKAEE--EE 153
Query: 79 LLRRTEKRAFAAFKRAASLVDSSPASVPLPLRVEPKPKSGIRQQDLLKKVVEIKPKRPRS 138
++R ++ + A L D S L +E EI K
Sbjct: 154 VIRLRAEQLGSITAADAGLDDDSEEDSDRELTME-----------------EISDK---- 192
Query: 139 ESKQSTSASSDVSVTNRKPDHGRLKETGQSLSGVKKVEEQSL--SGSTKVEDLSSGSHDA 196
KQ+T + +D K H ++E + ++ + K E+ + S + ++ L S +DA
Sbjct: 193 -GKQATKSITDKKEKGDKDTH--VEEIKKDINSLSKEEQMDVVYSSAPEIVGLLSELNDA 249
Query: 197 --DAKPKIN 203
+ + KIN
Sbjct: 250 VEELESKIN 258
>At1g16630 Hypothetical protei
Length = 845
Score = 30.8 bits (68), Expect = 0.59
Identities = 16/45 (35%), Positives = 25/45 (55%)
Query: 143 STSASSDVSVTNRKPDHGRLKETGQSLSGVKKVEEQSLSGSTKVE 187
S S+SSD ++ + + G+ +E S GV VEEQ G ++E
Sbjct: 259 SESSSSDTDLSAEREEEGQQEEEVASQEGVVAVEEQEDDGEERLE 303
>At2g12090 pseudogene; similar to MURA transposase of maize Mutator
transposon
Length = 248
Score = 30.4 bits (67), Expect = 0.77
Identities = 27/110 (24%), Positives = 47/110 (42%), Gaps = 7/110 (6%)
Query: 71 EAEEDAERLLRRTEKRAFAAFKRAASLVDSSPASVPLPLRVEPKPKSGIRQQDLLKKVVE 130
E E++ E+ E+ + ++ + S DS A L + RQ D L V+E
Sbjct: 127 EVEDENEKEEASEEEESRSSSQTLGSDSDSEEAETNEELACANPVEEEERQDDGLTAVIE 186
Query: 131 IKPKRPRSESKQSTSASSDVSVTNRKPDHGRLKETGQSLSGVKKVEEQSL 180
E + S+++ DV+V D G E + + K VEE+++
Sbjct: 187 -------EEEESSSTSDKDVNVEKSVEDKGDEDERDEDVIVEKPVEERTI 229
>At1g16270 putative Ser/Thr protein kinase
Length = 1147
Score = 30.4 bits (67), Expect = 0.77
Identities = 23/73 (31%), Positives = 35/73 (47%), Gaps = 14/73 (19%)
Query: 99 DSSPASVPLPLRVEPKPKSGIRQQDLLKKVVE--IKPK-------RPRSE-----SKQST 144
+SS S+P+P +P P GI QQ+ +V E IKP+ P + + Q+
Sbjct: 436 NSSNYSIPIPFPGQPYPHPGITQQNAPVQVEEPNIKPETKVRDYVEPENRHILATNHQNP 495
Query: 145 SASSDVSVTNRKP 157
+ D V NR+P
Sbjct: 496 PQADDTEVKNREP 508
>At4g21550 VP1 like protein
Length = 739
Score = 30.0 bits (66), Expect = 1.0
Identities = 27/104 (25%), Positives = 44/104 (41%), Gaps = 7/104 (6%)
Query: 67 KEQMEAEEDAERLLRRTEKRAFAAFKRAASLVDS-----SPASVPLPLRVEPKPKSGIRQ 121
K+QME E++A +LL + ++A+ ++ SP V L L +P+
Sbjct: 626 KKQMEKEDNARKLLEQLNSDN--GLHQSANNSENHERHASPLKVQLDLNFKPEKDEESLP 683
Query: 122 QDLLKKVVEIKPKRPRSESKQSTSASSDVSVTNRKPDHGRLKET 165
E P +S ++ +SS N K D G+LK T
Sbjct: 684 GSNKTTKSETLPHDDTVKSSFTSPSSSSAHSQNNKEDEGKLKTT 727
>At3g63510 unknown protein
Length = 404
Score = 30.0 bits (66), Expect = 1.0
Identities = 25/102 (24%), Positives = 44/102 (42%), Gaps = 5/102 (4%)
Query: 35 QRMVAKMQEVAGERGGYLHGRGALDSDDLLYLKEQMEAEEDAERLLRRTEKRAFAAFKRA 94
+R V + +V G+ HG G + DL+ + E+ L +R AF +
Sbjct: 292 RRQVLEQYQVYGDSVLGTHGNGRPNVRDLVKPLLNLFHSENGNSLWKRRADAAFKECRSV 351
Query: 95 ASLVDSSPASVP---LPLRVEPKPKSGIRQQDLLKKVVEIKP 133
SL++ S ++P L + P+SG +D+ V + P
Sbjct: 352 GSLLEESLRAIPDCVLDSPISGSPESG--DEDVFADVHNVLP 391
>At3g02810 putative protein kinase
Length = 558
Score = 30.0 bits (66), Expect = 1.0
Identities = 25/103 (24%), Positives = 42/103 (40%), Gaps = 9/103 (8%)
Query: 99 DSSPASVPLPLRVEPKPKSGIRQQDLLKKVVEIKPKRPRSESKQSTSASSDVSVTNRKPD 158
D P +VP+ + + + D +V P+ + K STS+ + S+ + K
Sbjct: 349 DGIPTTVPILSFKDKSMSIALSRHD--SNLVSPPPELATEDDKSSTSSGEESSLESEK-- 404
Query: 159 HGRLKETGQSLSGVKKVEEQSLSGSTKVEDLSSGSHDADAKPK 201
E+ KK EE+ S + E S+ H+ D PK
Sbjct: 405 -----ESVSKNEYKKKHEEEDSSMESDDESDSNSEHEKDQPPK 442
>At4g30160 putative villin
Length = 974
Score = 29.6 bits (65), Expect = 1.3
Identities = 20/75 (26%), Positives = 35/75 (46%), Gaps = 5/75 (6%)
Query: 121 QQDLLKKVVEIKPKRPRSESKQSTSASSDVSVTNRKPDHGRLKETGQSLSGVKKVEEQSL 180
Q+ + K V+ PK P S + +S S + N K + G + +SL+ ++E +
Sbjct: 849 QEPSIPKPVKASPKTPESPAPESNSKEQEEKKENDK-EEGSMSSRIESLT----IQEDAK 903
Query: 181 SGSTKVEDLSSGSHD 195
G EDL + +D
Sbjct: 904 EGVEDEEDLPAHPYD 918
>At5g16730 putative protein
Length = 853
Score = 29.3 bits (64), Expect = 1.7
Identities = 42/159 (26%), Positives = 71/159 (44%), Gaps = 26/159 (16%)
Query: 77 ERLLRRTEKRAFAAFKRAASLVDS----SPASVPLPLRV-EPKPKSGIRQQDLLKKVVE- 130
+ LL+RTE+ A AA+K+ A DS V L + E K +S +++LL K E
Sbjct: 574 DNLLKRTEEEADAAWKKEAQTKDSLKEVEEEIVYLQETLGEAKAESMKLKENLLDKETEF 633
Query: 131 ---------IKPKRPRSESK--QSTSASSDVSVTNRKP--DHGRLKETGQSLSGVKKVEE 177
+K K S K + + + + ++P ++G L E+ + + KV E
Sbjct: 634 QNVIHENEDLKAKEDVSLKKIEELSKLLEEAILAKKQPEEENGELSESEKDYDLLPKVVE 693
Query: 178 -QSLSGSTKVEDLSSGSHDADAKP------KINNSGGGL 209
S +G VE+ S+ D +P N++G G+
Sbjct: 694 FSSENGHRSVEEKSAKVETLDHEPPQEQISNGNSNGNGM 732
>At3g44040 putative protein
Length = 144
Score = 29.3 bits (64), Expect = 1.7
Identities = 12/32 (37%), Positives = 22/32 (68%)
Query: 127 KVVEIKPKRPRSESKQSTSASSDVSVTNRKPD 158
KV + KP++P+SE Q+ S S V+ +++P+
Sbjct: 35 KVAQTKPQQPKSEKSQNRSIGSVVNRPDKQPN 66
>At1g77180 unknown protein
Length = 613
Score = 28.9 bits (63), Expect = 2.2
Identities = 53/218 (24%), Positives = 75/218 (34%), Gaps = 30/218 (13%)
Query: 4 REVREYTNLSDPKDKKWGKGKDKIDDEDVTFQRMVAKMQEVAGERGGYLHGRGALDSDDL 63
R RE D KD GK D D VA G +GG G + D
Sbjct: 408 RRERERERRLDAKDAAMGKKSKITRDRDRDISEKVALGMASTGGKGG-----GEVMYDQR 462
Query: 64 LYLKEQ-MEAEEDAERLLRRTEKRAFAAFKRAASLVDSSPASVPLPLRVEPKPKSGIRQQ 122
L+ +++ M++ A+ +K F A ++L P E + Q
Sbjct: 463 LFNQDKGMDSGFAADDQYNLYDKGLFTAQPTLSTLYK------PKKDNDEEMYGNADEQL 516
Query: 123 DLLKKVVEIKPKRPRSESKQSTSASSDVSVTNRKPDHGRLKETGQSLSGVKKVEEQSLSG 182
D +K KP K T AS V +P KE Q G++K G
Sbjct: 517 DKIKNTERFKP------DKAFTGASERVGSKRDRPVEFE-KEEEQDPFGLEKWVSDLKKG 569
Query: 183 STKVEDLSSGSHDADAKPKINNSGGGLLGLAYASSDDD 220
++ + SG + SGGG +S DDD
Sbjct: 570 KKPLDKIGSGG-------TMRASGGG----GSSSRDDD 596
>At5g65900 ATP-dependent RNA helicase-like
Length = 633
Score = 28.5 bits (62), Expect = 2.9
Identities = 17/55 (30%), Positives = 29/55 (51%), Gaps = 2/55 (3%)
Query: 130 EIKPKRPRSESKQSTSASSDVSVTNRKPDHGRLKETGQSLSGVKKVEEQSLSGST 184
E +PK+ + + ++ T A S+ K + +L+ET S+ K E SLS +T
Sbjct: 113 EEEPKKKKKKQRKDTEAKSEEEEVEDKEEEKKLEET--SIMTNKTFESLSLSDNT 165
>At5g47680 unknown protein
Length = 344
Score = 28.5 bits (62), Expect = 2.9
Identities = 20/84 (23%), Positives = 35/84 (40%), Gaps = 10/84 (11%)
Query: 102 PASVPLPLRVEPKPKSGIRQQDLLKKVVEIKPKRPRSESKQSTSASSDVSVTNRKPDHGR 161
P S P P+ V P +++ L ++ E K +++ K+ RK +
Sbjct: 16 PESEPAPVNVSPPLSKNAQKKQLKQQRYEAKKAEKKAQEKEH----------KRKEGERK 65
Query: 162 LKETGQSLSGVKKVEEQSLSGSTK 185
LKE ++L+ + E L S K
Sbjct: 66 LKEWEETLANATEEERLKLIESRK 89
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.307 0.127 0.342
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,142,218
Number of Sequences: 26719
Number of extensions: 225360
Number of successful extensions: 728
Number of sequences better than 10.0: 58
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 49
Number of HSP's that attempted gapping in prelim test: 714
Number of HSP's gapped (non-prelim): 61
length of query: 221
length of database: 11,318,596
effective HSP length: 95
effective length of query: 126
effective length of database: 8,780,291
effective search space: 1106316666
effective search space used: 1106316666
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 43 (22.0 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0383.4