BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0346.3
(374 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g18410 hypothetical protein 321 5e-88
At5g35910 nucleolar protein-like 37 0.018
At5g22310 unknown protein 34 0.11
At4g38480 unknown protein 33 0.19
At3g57180 unknown protein 33 0.19
At5g13110 glucose-6-phosphate 1-dehydrogenase 32 0.56
At3g29000 unknown protein 32 0.56
At3g11240 arginine tRNA like protein transferase 32 0.74
At3g50550 unknown protein 31 0.96
At4g02740 hypothetical protein 31 1.3
At3g07750 putative 3' exoribonuclease 31 1.3
At2g01290 putative ribose 5-phosphate isomerase 30 1.6
At5g64420 putative protein 30 2.1
At5g42280 putative protein 30 2.1
At4g03760 hypothetical protein 30 2.1
At3g50690 putative protein 30 2.1
At3g49140 putative protein 30 2.1
At1g71100 ribose 5-phosphate isomerase like protein 30 2.1
At1g49900 Cys2/His2-type zinc finger protein, putative 30 2.1
At5g63740 unknown protein 29 3.7
>At2g18410 hypothetical protein
Length = 392
Score = 321 bits (822), Expect = 5e-88
Identities = 200/413 (48%), Positives = 252/413 (60%), Gaps = 60/413 (14%)
Query: 1 MAESICRTLRDGALEGELAPTLTITDSLTSPLASDVFSHLLLQLSSHLIASKSHSQFRSG 60
MAESI R LRDG EGELAP LTI +++ SP DV +LL LSS ++A KS SQ G
Sbjct: 1 MAESIFRKLRDGGEEGELAPALTIEETVASPFGLDVSGYLLTNLSSSILAGKSSSQ---G 57
Query: 61 IVIVALSRTPSSYAALFKTKGIHISSSNNNWIHILDCYTDPLGWKEKPRKSQNVTNPSHQ 120
+V++ SR+PS Y L K KGI +SSS+ WI ILDCYTDPLGW ++ S + + S
Sbjct: 58 LVLITFSRSPSFYLQLLKQKGIVVSSSSK-WIRILDCYTDPLGWIDQ--SSTSFSEGSSL 114
Query: 121 VSLATTSYKTVKDLDKLFSVITELGR------------------GLIGENKARFCVAIDS 162
+ L +K V DL KLFS I E GR L+G K RFCVAIDS
Sbjct: 115 IKL----HKCVSDLKKLFSSIIEAGRVRSLSKGLYEIKSDCFGAELVGTGKTRFCVAIDS 170
Query: 163 LSELLRHASLQSVAGLLSNLRSHDQISSIFGLLHSDLHEERAAAVLEYMSSMVASVAPLH 222
E++ + + +S QISS+F L++DLH+E+ LEY+S+M A++ PL
Sbjct: 171 KREVIIYLNFESA-----------QISSVFWSLNTDLHQEKVTNALEYISTMKANLEPLC 219
Query: 223 HSAE--RGNLENSLS-EQNFTKGRLNVRFKRRNGRVRVTCEEFKVEAGGISLTSVSSADG 279
S++ R LEN S Q+F KGR +VRFK R GRVRV EE+ V+ GI+ + +SS D
Sbjct: 220 PSSDGQRNALENLFSVHQDFGKGRFHVRFKLRKGRVRVMSEEYHVDQSGINFSPISSVDT 279
Query: 280 ATVA--GLLPK-----------VQFNLQLSEKEKTDRAKVVLPFEHQGTGRPIQIYDGRR 326
A LLPK VQFNLQLSEKE+ ++ KVVLPFEHQ G+ +IYDGRR
Sbjct: 280 VIAATKSLLPKASYLDLLFNYLVQFNLQLSEKERVEKEKVVLPFEHQDDGKSNEIYDGRR 339
Query: 327 SLDDNSSEAAPISSGKKE-----NSEMGEIIYFRDSDDEMPDSDEDPDDDLDI 374
SL D E P+SS + + + + GEIIYFRDSDDE PDSDEDPDDDLDI
Sbjct: 340 SLVDGKIETTPLSSMELQTDVVSSGKGGEIIYFRDSDDEHPDSDEDPDDDLDI 392
>At5g35910 nucleolar protein-like
Length = 834
Score = 37.0 bits (84), Expect = 0.018
Identities = 19/53 (35%), Positives = 31/53 (57%), Gaps = 7/53 (13%)
Query: 323 DGRRSLDDNSSEAAPI-------SSGKKENSEMGEIIYFRDSDDEMPDSDEDP 368
DG + L D+S EA+ I S K +SEMG+II + D++ D++++P
Sbjct: 687 DGVKELKDDSEEASEIVGTSGRVSESKVSSSEMGDIILLENGDEKKVDAEDEP 739
>At5g22310 unknown protein
Length = 481
Score = 34.3 bits (77), Expect = 0.11
Identities = 49/234 (20%), Positives = 89/234 (37%), Gaps = 24/234 (10%)
Query: 160 IDSLSELLRHASLQSVAGLLSNL-RSHDQISSIFGLLHSDLHEERAAAVLEYMSSMVASV 218
I L + + AS + ++ LL L R+ + + L D EE ++E +
Sbjct: 199 IGELGDDHKTASNRLISALLCELDRARSSLKHLMSEL--DEEEEEKRRLIESLQEEAMVE 256
Query: 219 APLHHSAERGN--LENSLSEQNFTKGRLNVRFKRRNGRVRV---TCEEFKVEAGGISLTS 273
L E+ N L L+E T+ ++ KR V C+E G
Sbjct: 257 RKLRRRTEKMNRRLGRELTEAKETERKMKEEMKREKRAKDVLEEVCDELTKGIGDDKKEM 316
Query: 274 VSSADGATVAGLLPKVQFNLQLSEKE-------------KTDRAKVVLPFEHQGTG---R 317
+ +A +L + + ++L+E + K + +V+ E +G+ R
Sbjct: 317 EKEREMMHIADVLREERVQMKLTEAKFEFEDKYAAVERLKKELRRVLDGEEGKGSSEIRR 376
Query: 318 PIQIYDGRRSLDDNSSEAAPISSGKKENSEMGEIIYFRDSDDEMPDSDEDPDDD 371
+++ DG S DD S+ I + S+ G + +D +D DDD
Sbjct: 377 ILEVIDGSGSDDDEESDLKSIELNMESGSKWGYVDSLKDRRRFDGSGGDDDDDD 430
>At4g38480 unknown protein
Length = 471
Score = 33.5 bits (75), Expect = 0.19
Identities = 17/45 (37%), Positives = 21/45 (45%)
Query: 329 DDNSSEAAPISSGKKENSEMGEIIYFRDSDDEMPDSDEDPDDDLD 373
DD SSE + E E GE+ DD+ D+D DDD D
Sbjct: 417 DDESSEESSDDDDSSEEEENGEVDVEITKDDDNDHGDDDDDDDED 461
>At3g57180 unknown protein
Length = 637
Score = 33.5 bits (75), Expect = 0.19
Identities = 43/160 (26%), Positives = 66/160 (40%), Gaps = 28/160 (17%)
Query: 229 NLENSLSEQNFTKGRL----NVRF-------KRRNGRVRVTCEEFKVEAGGISLTSVSSA 277
N++ L + NF RL V F K++ V V C K E + SV S
Sbjct: 13 NVKPKLEDGNFRVSRLIHRPEVPFFSGLSNEKKKKCAVSVMCLAVKKEQ---VVQSVESV 69
Query: 278 DGATVAGLLPKVQFNLQLSE--KEKTDRAKVVLPFEHQGTGRPIQIYDGRRSLDDNSSEA 335
+G + PK NL +SE E D K++ P G I + D L +
Sbjct: 70 NGT----IFPKKSKNLIMSEGRDEDEDYGKIICP------GCGIFMQDNDPDLPGYYQKR 119
Query: 336 APISSGKK--ENSEMGEIIYFRDSDDEMPDSDEDPDDDLD 373
I++ + E+ E E+ F DD+ + +E DD++D
Sbjct: 120 KVIANNLEGDEHVENDELAGFEMVDDDADEEEEGEDDEMD 159
>At5g13110 glucose-6-phosphate 1-dehydrogenase
Length = 596
Score = 32.0 bits (71), Expect = 0.56
Identities = 25/80 (31%), Positives = 37/80 (46%), Gaps = 5/80 (6%)
Query: 210 YMSSMVASVAPLHHSAERGNLENSLSEQNFTKGRLNVRFKRRNGRVRVTCEEFKVEAGGI 269
Y S +AS +P++ R S S Q L VRF+R++GR V ++ G I
Sbjct: 13 YHSGYLASFSPVNGDRHRSLSFLSASPQGLNPLDLCVRFQRKSGRASVFMQD-----GAI 67
Query: 270 SLTSVSSADGATVAGLLPKV 289
S SS ++ GL +V
Sbjct: 68 VTNSNSSESKTSLKGLKDEV 87
>At3g29000 unknown protein
Length = 194
Score = 32.0 bits (71), Expect = 0.56
Identities = 15/34 (44%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Query: 342 KKENSEMGEIIYFR-DSDDEMPDSDEDPDDDLDI 374
KK N + E + + D DD+ D D+D DDD+DI
Sbjct: 50 KKNNKDQQETLTKQEDDDDDDDDDDDDDDDDIDI 83
>At3g11240 arginine tRNA like protein transferase
Length = 605
Score = 31.6 bits (70), Expect = 0.74
Identities = 18/45 (40%), Positives = 24/45 (53%), Gaps = 3/45 (6%)
Query: 327 SLDDNSSEAAPISSGKKENSEMGEIIYFRDSDDEMPDSDEDPDDD 371
S +SE S+ + E+ E G+ D DDEM +SDED D D
Sbjct: 501 SSSPQASETLLESTSEHEDMEQGDT---NDDDDEMYNSDEDSDSD 542
>At3g50550 unknown protein
Length = 95
Score = 31.2 bits (69), Expect = 0.96
Identities = 17/49 (34%), Positives = 25/49 (50%), Gaps = 4/49 (8%)
Query: 323 DGRRSLDDNSSEAAPISSGKKENSEMGEIIYFRDSDDEMPDSDEDPDDD 371
DG S +D+ E +S E+SE + DS+ + D DE+ DDD
Sbjct: 28 DGESSDEDDEEEDRNLSGDDSESSEDD----YTDSNSDSDDDDEEDDDD 72
>At4g02740 hypothetical protein
Length = 645
Score = 30.8 bits (68), Expect = 1.3
Identities = 18/59 (30%), Positives = 27/59 (45%)
Query: 313 QGTGRPIQIYDGRRSLDDNSSEAAPISSGKKENSEMGEIIYFRDSDDEMPDSDEDPDDD 371
QGT P + G+R + E I + E+ E+ E D D++ D DE +DD
Sbjct: 305 QGTSNPFGKFSGKRYGVCDEEELRLIEMMEAEDDEVDEEDDSDDDTDDVSDEDESENDD 363
>At3g07750 putative 3' exoribonuclease
Length = 286
Score = 30.8 bits (68), Expect = 1.3
Identities = 29/104 (27%), Positives = 46/104 (43%), Gaps = 4/104 (3%)
Query: 233 SLSEQNFTKGRLNVRFKRRNGRVRVTCEEFKVEAGGISLTSVSSADGATVAGLLPKVQFN 292
SL EQ+F KG + + R +GR R+T VE G I + S+ ++ V+
Sbjct: 4 SLGEQHFIKGGI-AQDLRTDGRKRLTYRPIYVETGVIPQANGSARVRIGGTDVIASVKAE 62
Query: 293 LQLSEKEKTDRAKVVLPFEHQGTGRPIQIYDGRRSLDDNSSEAA 336
+ + D+ KV + + T P G R ++ SSE A
Sbjct: 63 IGRPSSLQPDKGKVAVFIDCSPTAEPT---FGGRGGEELSSELA 103
>At2g01290 putative ribose 5-phosphate isomerase
Length = 265
Score = 30.4 bits (67), Expect = 1.6
Identities = 20/73 (27%), Positives = 41/73 (55%), Gaps = 9/73 (12%)
Query: 127 SYKTVKDLDKLFSVITELGRGLIGENKARFCVAIDSLSELLRHASLQSVAGLLSNLRSHD 186
+YK V+ ++ ++ LG G ++ A+D + ELLR L+++ G+ ++ ++ +
Sbjct: 41 AYKAVEFVES--GMVLGLGTGSTAKH------AVDRIGELLRQGKLENIVGIPTSKKTQE 92
Query: 187 QISSIFGLLHSDL 199
Q S+ G+ SDL
Sbjct: 93 QALSL-GIPLSDL 104
>At5g64420 putative protein
Length = 1306
Score = 30.0 bits (66), Expect = 2.1
Identities = 17/59 (28%), Positives = 26/59 (43%), Gaps = 15/59 (25%)
Query: 328 LDDNSSEAAPISSGKKENSEMGEI---------------IYFRDSDDEMPDSDEDPDDD 371
LDD+ + I ++EN EMGE + D E+P++ +D DDD
Sbjct: 910 LDDDEEDCLAIEDAEEENEEMGETGESDEQTDDSEAVTGVVPMAVDREVPENSDDSDDD 968
>At5g42280 putative protein
Length = 694
Score = 30.0 bits (66), Expect = 2.1
Identities = 15/31 (48%), Positives = 18/31 (57%)
Query: 343 KENSEMGEIIYFRDSDDEMPDSDEDPDDDLD 373
K+ S E IY D D E D+D+D DDD D
Sbjct: 141 KDKSSTEESIYEVDDDCEDDDNDDDDDDDDD 171
>At4g03760 hypothetical protein
Length = 723
Score = 30.0 bits (66), Expect = 2.1
Identities = 24/105 (22%), Positives = 43/105 (40%), Gaps = 10/105 (9%)
Query: 249 KRRNGRVRVTCEEFKVEAGGISLTSVSSADGATVAGLLPKVQFNLQL--SEKEKTD---- 302
K++ + R EE ++ G+ L V+S D + P+V + SE ++ D
Sbjct: 399 KKKEDKERRLAEEKRLANAGLILPRVASPDATQDRNVAPEVAAPVDPTPSEAQEVDPTAV 458
Query: 303 ----RAKVVLPFEHQGTGRPIQIYDGRRSLDDNSSEAAPISSGKK 343
A VVLP G+ ++ D +A+ +GK+
Sbjct: 459 APLPEAVVVLPASDMAAGKRVRTNDSSSKKKSKKKKASSAEAGKE 503
>At3g50690 putative protein
Length = 447
Score = 30.0 bits (66), Expect = 2.1
Identities = 15/78 (19%), Positives = 40/78 (51%)
Query: 296 SEKEKTDRAKVVLPFEHQGTGRPIQIYDGRRSLDDNSSEAAPISSGKKENSEMGEIIYFR 355
+E +++++A V ++ G ++ +G +D+ ++ E++++ E+
Sbjct: 225 AEDDESEQATGVNGTSYRANGFRLEAVNGEEVREDDGDDSESGEEEVGEDNDVVEVHEIE 284
Query: 356 DSDDEMPDSDEDPDDDLD 373
DS++E D++ DD+ D
Sbjct: 285 DSENEEDGVDDEEDDEED 302
>At3g49140 putative protein
Length = 1229
Score = 30.0 bits (66), Expect = 2.1
Identities = 17/55 (30%), Positives = 25/55 (44%), Gaps = 9/55 (16%)
Query: 328 LDDNSSEAAPISSGKKENSEMGE---------IIYFRDSDDEMPDSDEDPDDDLD 373
L D+ E SG ++ + GE I+ D DD+ D D++ DDD D
Sbjct: 934 LSDSDFETEDDESGDDDSEDTGEDEDEEEWVAILEDEDEDDDDDDDDDEDDDDSD 988
>At1g71100 ribose 5-phosphate isomerase like protein
Length = 267
Score = 30.0 bits (66), Expect = 2.1
Identities = 21/73 (28%), Positives = 40/73 (54%), Gaps = 9/73 (12%)
Query: 127 SYKTVKDLDKLFSVITELGRGLIGENKARFCVAIDSLSELLRHASLQSVAGLLSNLRSHD 186
+YK V+ ++ ++ LG G ++ A+ +SELLR L+ + G+ ++ +H+
Sbjct: 37 AYKAVEFVES--GMVIGLGTGSTAKH------AVARISELLREGKLKDIIGIPTSTTTHE 88
Query: 187 QISSIFGLLHSDL 199
Q S+ G+ SDL
Sbjct: 89 QAVSL-GIPLSDL 100
>At1g49900 Cys2/His2-type zinc finger protein, putative
Length = 917
Score = 30.0 bits (66), Expect = 2.1
Identities = 12/21 (57%), Positives = 14/21 (66%)
Query: 353 YFRDSDDEMPDSDEDPDDDLD 373
Y+ D DD+ D DED DDD D
Sbjct: 640 YYDDDDDDDDDGDEDDDDDDD 660
>At5g63740 unknown protein
Length = 226
Score = 29.3 bits (64), Expect = 3.7
Identities = 15/55 (27%), Positives = 25/55 (45%), Gaps = 2/55 (3%)
Query: 319 IQIYDGRRSLDDNSSEAAPISSGKKENSEMGEIIYFRDSDDEMPDSDEDPDDDLD 373
I++YD + D + G ++ E + D D++ D D+D DDD D
Sbjct: 49 IKVYDDHNN--DGEGDGDGDGDGDEDEDEDADADEDEDEDEDEDDDDDDDDDDDD 101
Score = 28.1 bits (61), Expect = 8.1
Identities = 12/18 (66%), Positives = 12/18 (66%)
Query: 356 DSDDEMPDSDEDPDDDLD 373
D DDE D DED DDD D
Sbjct: 111 DDDDEDDDEDEDDDDDDD 128
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.314 0.131 0.362
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,064,786
Number of Sequences: 26719
Number of extensions: 337523
Number of successful extensions: 1591
Number of sequences better than 10.0: 39
Number of HSP's better than 10.0 without gapping: 21
Number of HSP's successfully gapped in prelim test: 21
Number of HSP's that attempted gapping in prelim test: 1361
Number of HSP's gapped (non-prelim): 134
length of query: 374
length of database: 11,318,596
effective HSP length: 101
effective length of query: 273
effective length of database: 8,619,977
effective search space: 2353253721
effective search space used: 2353253721
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0346.3