BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0344.11
(579 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g45570 putative protein 35 0.15
At5g07660 SMC-like protein 35 0.15
At1g44780 hypothetical protein 32 0.74
At1g70320 hypothetical protein 32 0.97
At4g33060 cyclophilin (AtCYP57) 32 1.3
At5g60210 putative protein 31 1.7
At5g61070 HDA18 31 2.2
At1g79280 hypothetical protein 31 2.2
At3g42690 putative protein 30 2.8
At5g20750 putative protein 30 3.7
At1g55860 ubiquitin-protein ligase 1, putative 30 3.7
At4g01300 hypothetical protein 30 4.8
At3g57320 unknown protein 30 4.8
At3g30560 hypothetical protein 30 4.8
At1g06530 hypothetical protein 30 4.8
At5g65160 unknown protein 29 6.3
At5g17080 putative protein 29 6.3
At4g04130 putative protein 29 6.3
At5g23960 beta-caryophyllene/alpha-humulene synthase 29 8.2
At5g02250 ribonuclease II-like protein 29 8.2
>At5g45570 putative protein
Length = 921
Score = 34.7 bits (78), Expect = 0.15
Identities = 30/135 (22%), Positives = 62/135 (45%), Gaps = 10/135 (7%)
Query: 248 YAQLKYIYVPINAGYDHYYLMVIALSERRVYHFDSFP----DEDTSFYKQFFMRQIRKMI 303
+ + ++Y + +H+ + I L+ +RV +DS P D + + F M I M+
Sbjct: 765 FVDVDHLYAYLFVNGNHWVALDIDLTNKRVNVYDSIPSLTTDTEMAIQCMFVMTMIPAML 824
Query: 304 QDLLALDKFSSMESNGKVDLENWEVVELTVDGGESYMYNSPIWVMEWLSMGSSFHQNAKT 363
+ + S K++ + + +D G+ +Y+ I +E L++G SF +
Sbjct: 825 SSFIPSKQ--RRRSYSKLEWKRITKIPENLDPGDCAIYS--IKYIECLALGKSF--DGLC 878
Query: 364 KLNERASRMKIAVNL 378
N ++ R K+AV +
Sbjct: 879 DENMQSLRTKLAVEM 893
>At5g07660 SMC-like protein
Length = 1058
Score = 34.7 bits (78), Expect = 0.15
Identities = 42/167 (25%), Positives = 69/167 (41%), Gaps = 25/167 (14%)
Query: 355 SSFHQNAKTKLNERASRMKIAVNLVGGVHNECWKELEGKVDKFWNMVKPIGPPITLDQRL 414
+SF K L ASR + + G E LEG + K QR
Sbjct: 635 ASFDDQIKD-LEIEASREQSEIQECRGQKREAEMNLEGLESTMRRLKK---------QRT 684
Query: 415 FLDPHVL--DIDLPSSRTSVPASMSASGTHSV-------------IAELEAISKELQETI 459
L+ + ++++ + SV + AS T SV I E E++ ++LQ+++
Sbjct: 685 QLEKDLTRKELEMQDLKNSVASETKASPTSSVNELHLEIMKFQKEIEEKESLLEKLQDSL 744
Query: 460 RIAAARKIKVDALIQTLKEEAGQEGEHAADAEREGSAENDEEHSSSS 506
+ A + ++ A + L E A E E AE E + DE HS+ +
Sbjct: 745 KEAELKANELKASYENLYESAKGEIEALEKAEDELKEKEDELHSAET 791
>At1g44780 hypothetical protein
Length = 471
Score = 32.3 bits (72), Expect = 0.74
Identities = 16/68 (23%), Positives = 37/68 (53%), Gaps = 6/68 (8%)
Query: 451 ISKELQETIRIAAARKIKVDALIQTLKEEAGQ------EGEHAADAEREGSAENDEEHSS 504
I+KEL E +++ A K +++++ +K++ E+ +D++ EG+ +N+E
Sbjct: 191 INKELDEVLQLPDAPKCSTESIVKNVKKKVKSTPSKMVSSEYNSDSDTEGNVDNEEVAVK 250
Query: 505 SSVCSFVK 512
++ VK
Sbjct: 251 KTMARKVK 258
>At1g70320 hypothetical protein
Length = 3658
Score = 32.0 bits (71), Expect = 0.97
Identities = 22/80 (27%), Positives = 40/80 (49%), Gaps = 3/80 (3%)
Query: 440 GTHSVIAELEAISKELQETIRIAAARKIKVDALIQTLKEEAGQEGEHAAD---AEREGSA 496
G+ SVI+EL A + ++ + + I + I AL KE+ + + A+D + +A
Sbjct: 845 GSASVISELSAANADVLKELGITYKQTIWQMALCNDTKEDEKKSVDRASDNSVSASSSTA 904
Query: 497 ENDEEHSSSSVCSFVKTRPV 516
E + + SS+ + T PV
Sbjct: 905 ERESDEDSSNALAVRYTNPV 924
>At4g33060 cyclophilin (AtCYP57)
Length = 504
Score = 31.6 bits (70), Expect = 1.3
Identities = 31/124 (25%), Positives = 53/124 (42%), Gaps = 20/124 (16%)
Query: 73 IDSSDDEDVPRTATHLQKQVKTEVLERQHELITPQGKSFLQFEQNKVFGSGKDSYQRAPK 132
+ +SDD+D T +++ +VL R+ E+ K + + + + G ++S QR+
Sbjct: 282 VGNSDDDDDGEDETKFDAKMRNQVLSRRKEIGDTPSKPTQKKKSSSLKGR-EESTQRSDA 340
Query: 133 KSMED------SLARKRLPYTPEAKRPKLEPN-------------LQSHKLIKTKFKPSE 173
S ED L+ K+ EAK +E Q HKL K + + +E
Sbjct: 341 VSSEDEKPRMEKLSLKKKGIGSEAKAEHMEKGDTDLQLYNASERARQLHKLKKRRLQGNE 400
Query: 174 DMKL 177
D L
Sbjct: 401 DSVL 404
>At5g60210 putative protein
Length = 629
Score = 31.2 bits (69), Expect = 1.7
Identities = 27/91 (29%), Positives = 47/91 (50%), Gaps = 9/91 (9%)
Query: 428 SRTSVPASMSASGTHSVIAELEAISKELQETIRIAAARKIKVDALIQTLKEEAGQEGEHA 487
+R S + +S S I ELE + +LQE ++ A +I V +T K++A QE E +
Sbjct: 79 NRRSPRSPVSEKKRPSRITELELLVSQLQEELK-KAKDQISVS---ETSKKQAEQEAEES 134
Query: 488 ADAEREGSAENDEEHSSSSVCSFVKTRPVQE 518
+E S++ +E + FV+T ++E
Sbjct: 135 RKQLQEVSSKLEESQN-----QFVETSALEE 160
>At5g61070 HDA18
Length = 682
Score = 30.8 bits (68), Expect = 2.2
Identities = 23/61 (37%), Positives = 33/61 (53%), Gaps = 4/61 (6%)
Query: 447 ELEAISKELQETIRIAAARKIKVDALIQTLKEEAGQEGEHAADAEREGSAENDEEHSSSS 506
+LEA KEL+ + + AR+ K+ A I+ L++E E A AER E E+ S S
Sbjct: 555 DLEAKEKELEARLMLVHAREDKIHAKIERLQQE---RDEAVAKAERI-DKELQEDRSRSR 610
Query: 507 V 507
V
Sbjct: 611 V 611
>At1g79280 hypothetical protein
Length = 2111
Score = 30.8 bits (68), Expect = 2.2
Identities = 38/156 (24%), Positives = 68/156 (43%), Gaps = 16/156 (10%)
Query: 28 EDFDTTLKEIATKVDELDLAQGCTSKANIHTDSPKWRLDDY-----NIFVIDSSDDEDVP 82
E+F+ LK TK ELDL K + TD K R+D+ NI + D + +D
Sbjct: 1328 ENFENLLK---TKQTELDLCMKEMEKLRMETDLHKKRVDELRETYRNIDIADYNRLKDEV 1384
Query: 83 RTATHLQKQVKTEVLERQHELITPQGKSFLQFEQNKVFGSGKDSYQRAPKKSMEDS-LAR 141
R K + + L+ Q K + + ++ KD +R +K ++D+ A+
Sbjct: 1385 RQLEEKLKAKDAHAEDCKKVLLEKQNK--ISLLEKELTNCKKDLSER--EKRLDDAQQAQ 1440
Query: 142 KRLPYTPEAKRPKLEPNLQSH---KLIKTKFKPSED 174
+ ++ +LE N + H + K K++ +D
Sbjct: 1441 ATMQSEFNKQKQELEKNKKIHYTLNMTKRKYEKEKD 1476
>At3g42690 putative protein
Length = 1009
Score = 30.4 bits (67), Expect = 2.8
Identities = 17/41 (41%), Positives = 22/41 (53%), Gaps = 4/41 (9%)
Query: 254 IYVPINAGYDHYYLMVIALSERRVYHFDSF----PDEDTSF 290
+YVP+N G H+ +VI L R + DSF PDE F
Sbjct: 872 VYVPMNWGCSHWVGLVINLRLRHIDILDSFMSPTPDEAVEF 912
>At5g20750 putative protein
Length = 944
Score = 30.0 bits (66), Expect = 3.7
Identities = 21/77 (27%), Positives = 35/77 (45%), Gaps = 8/77 (10%)
Query: 254 IYVPINAGYDHYYLMVIALSERRVYHFD----SFPDEDTSFYKQFFMRQIRKMIQDLLAL 309
+YVP+N G H+ +VI L R V D FPDE S ++ + +++ LL
Sbjct: 806 VYVPMNWGSCHWVGLVINLQLRNVLILDPFSAPFPDEQVS----VMIKPVTELLPSLLKR 861
Query: 310 DKFSSMESNGKVDLENW 326
++ N + +W
Sbjct: 862 FAIPALTENLTTEPFDW 878
>At1g55860 ubiquitin-protein ligase 1, putative
Length = 3891
Score = 30.0 bits (66), Expect = 3.7
Identities = 21/80 (26%), Positives = 38/80 (47%), Gaps = 3/80 (3%)
Query: 440 GTHSVIAELEAISKELQETIRIAAARKIKVDALIQTLKEEAGQEGEHAAD---AEREGSA 496
G+ SVI+EL A ++ + + I + I AL KE+ + + +D + +A
Sbjct: 1092 GSASVISELSAADADVLKELGITYKQTIWQMALCNDTKEDEKKSVDRGSDNSVSASSSTA 1151
Query: 497 ENDEEHSSSSVCSFVKTRPV 516
E + + SS+ + T PV
Sbjct: 1152 ERESDEDSSNALAVRYTNPV 1171
>At4g01300 hypothetical protein
Length = 291
Score = 29.6 bits (65), Expect = 4.8
Identities = 32/132 (24%), Positives = 51/132 (38%), Gaps = 15/132 (11%)
Query: 66 DDYNIFVIDSSDDEDVPRTATHLQKQVKTEVLERQHELITPQGKSF------LQFEQNKV 119
D +N SSD R +++ E+L ++H+ + + F+ ++
Sbjct: 160 DSFNDETFGSSDSTSEDRAEEERKRRASFELLRKEHQKAFQERQKSNPDLRKNDFDFTEL 219
Query: 120 FGSGKDSYQRAPKKSMEDSLA-------RKRLPYTPEAKRPKLEPNLQSHKLIKTK-FKP 171
G KD R P +S E + A LP A RP + P S L K + KP
Sbjct: 220 LGESKDDKGR-PSRSDEVNHAPTIPGSSNTSLPSQSNAPRPLVPPGFASTILEKKQGEKP 278
Query: 172 SEDMKLTPTQLH 183
L+ T +H
Sbjct: 279 QVSRYLSETLIH 290
>At3g57320 unknown protein
Length = 102
Score = 29.6 bits (65), Expect = 4.8
Identities = 14/43 (32%), Positives = 27/43 (62%)
Query: 4 LVLTKINRIDEAILVQNEAMTTKLEDFDTTLKEIATKVDELDL 46
L TK + D ++ +N+ + +KLE+ + T+K+ TKV +L +
Sbjct: 32 LASTKTDIPDAKLVEENKQLQSKLEEANATVKQEQTKVKDLTI 74
>At3g30560 hypothetical protein
Length = 1473
Score = 29.6 bits (65), Expect = 4.8
Identities = 17/53 (32%), Positives = 28/53 (52%), Gaps = 4/53 (7%)
Query: 91 QVKTEVLERQHELITPQGKSFLQFEQNKVFGSGKDSYQRAPKKSMEDSLARKR 143
Q+K+E L R HE S+L + +F G+D Y+ KK+ ++ RK+
Sbjct: 330 QIKSEKLRRIHECHV----SYLSLQYPLLFPKGEDGYRLGIKKTETNTSKRKK 378
>At1g06530 hypothetical protein
Length = 323
Score = 29.6 bits (65), Expect = 4.8
Identities = 22/99 (22%), Positives = 46/99 (46%), Gaps = 10/99 (10%)
Query: 7 TKINRIDEAILVQNEAMTTKLEDFDTTLKEIATKVDELDLAQGCTSKANIHTDSPKWRLD 66
T++N+ + QN+ + + + ++ + +++EL +G SKA K ++
Sbjct: 32 TELNQKIGDLESQNQELARDNDAINRKIESLTAEIEEL---RGAESKA-------KRKMG 81
Query: 67 DYNIFVIDSSDDEDVPRTATHLQKQVKTEVLERQHELIT 105
+ + S ++ V +++TEV QHELIT
Sbjct: 82 EMEREIDKSDEERKVLEAIASRASELETEVARLQHELIT 120
>At5g65160 unknown protein
Length = 593
Score = 29.3 bits (64), Expect = 6.3
Identities = 20/83 (24%), Positives = 37/83 (44%), Gaps = 3/83 (3%)
Query: 13 DEAILVQNEAMTTKLEDFDTTLKEIATKVD-ELDLAQGCTSKANIHTDSPKWRL--DDYN 69
+ +L A +KL FD ++++ + + +A+ + KW L DY
Sbjct: 503 NSVLLCNRAACRSKLGQFDKSIEDCTAALSVRPGYGKARLRRADCNAKIEKWELAVGDYE 562
Query: 70 IFVIDSSDDEDVPRTATHLQKQV 92
I +S +DE V R + Q+Q+
Sbjct: 563 ILKKESPEDEQVIRGLSEAQQQL 585
>At5g17080 putative protein
Length = 298
Score = 29.3 bits (64), Expect = 6.3
Identities = 20/75 (26%), Positives = 35/75 (46%), Gaps = 1/75 (1%)
Query: 368 RASRMKIAVNLVGGVHNECWKELEGKVDKF-WNMVKPIGPPITLDQRLFLDPHVLDIDLP 426
R S + ++ +H CW + GK+ +F +NM +PI + LD F + L D
Sbjct: 78 RQSHRALLSKIIEQIHAICWALVLGKILEFTYNMNRPIAQHMFLDIDSFAEKVKLKADEI 137
Query: 427 SSRTSVPASMSASGT 441
+++ V A G+
Sbjct: 138 NAQKKVITEDMAVGS 152
>At4g04130 putative protein
Length = 859
Score = 29.3 bits (64), Expect = 6.3
Identities = 15/41 (36%), Positives = 23/41 (55%), Gaps = 4/41 (9%)
Query: 254 IYVPINAGYDHYYLMVIALSERRVYHFDSF----PDEDTSF 290
+YVP+N G H+ +VI L ++ + DSF P+E F
Sbjct: 551 VYVPMNWGCSHWVGLVINLKQQSIDVLDSFISPTPEEAVEF 591
>At5g23960 beta-caryophyllene/alpha-humulene synthase
Length = 547
Score = 28.9 bits (63), Expect = 8.2
Identities = 46/218 (21%), Positives = 87/218 (39%), Gaps = 38/218 (17%)
Query: 206 SATVNDFDCLNPERVVEPEWDVRMGRKIEEIMDTYSNDWLHPYAQLKYIYVPINAGYDHY 265
+ TV +D L+ E +E E G +++ + +N ++ LK Y+ A +D Y
Sbjct: 340 NVTVEFYDKLDEE--LEKEGRSGCGFHLKKSLQKTANGYMQEAKWLKKDYI---ATFDEY 394
Query: 266 YLMVIALSERRVYHFDSFPDEDTSFYKQFFMRQIRKMIQDLLALDKFSSMESNGKVDLEN 325
I S +Y M +R + D+ LD F + S+ K+ + +
Sbjct: 395 KENAILSS---------------GYYALIAMTFVR--MTDVAKLDAFEWLSSHPKIRVAS 437
Query: 326 WEVVELTVDGGESYMYNSPIWVMEWLSMGSSFHQNAKTKLNERASRMKIAVNLVGGVHNE 385
E++ D SY + E ++ G + ERA V ++G + ++
Sbjct: 438 -EIISRFTDDISSYEFEHK---REHVATGIDCYMQQFGVSKERA------VEVMGNIVSD 487
Query: 386 CWKELEGKVDKFWNMVKPIGPPITLDQRLFLDPHVLDI 423
WK+L + +++P P L R+ V+D+
Sbjct: 488 AWKDLNQE------LMRPHVFPFPLLMRVLNLSRVIDV 519
>At5g02250 ribonuclease II-like protein
Length = 803
Score = 28.9 bits (63), Expect = 8.2
Identities = 23/76 (30%), Positives = 38/76 (49%), Gaps = 12/76 (15%)
Query: 424 DLPSSRTSVPASMSASGTHSVIAELEAISKELQETIRIAAARKIKVDALIQTLKE----- 478
D PS T + + + SV ELE+IS+ +R+ A+ K+K+ + + L++
Sbjct: 63 DAPSCSTCIHSLVE-----SVSEELESISRRKGSRMRVRASVKVKLTSYGEVLEDKLVNQ 117
Query: 479 --EAGQEGEHAADAER 492
EAG E DA+R
Sbjct: 118 ELEAGLLLEFKKDADR 133
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.318 0.134 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,119,315
Number of Sequences: 26719
Number of extensions: 574294
Number of successful extensions: 2153
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 20
Number of HSP's that attempted gapping in prelim test: 2142
Number of HSP's gapped (non-prelim): 31
length of query: 579
length of database: 11,318,596
effective HSP length: 105
effective length of query: 474
effective length of database: 8,513,101
effective search space: 4035209874
effective search space used: 4035209874
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 63 (28.9 bits)
Lotus: description of TM0344.11