BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0336.16
(135 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g21720 putative protein 191 8e-50
At5g25560 unknown protein 31 0.23
At3g12000 hypothetical protein 31 0.23
At3g19020 hypothetical protein 30 0.29
At4g38770 extensin - like protein 29 0.86
At3g51620 unknown protein 29 0.86
At4g12470 pEARLI 1-like protein 28 1.5
At5g10550 bromodomain protein - like 28 1.9
At3g54460 RING finger -like protein 28 1.9
At5g44680 unknown protein 27 2.5
At5g25590 unknown protein (At5g25590) 27 2.5
At2g24960 hypothetical protein 27 2.5
At5g35200 unknown protein 27 3.3
At3g14330 hypothetical protein 27 3.3
At2g45820 remorin 27 3.3
At1g20135 anter-specific proline-rich protein APG precursor 27 3.3
At5g09570 unknown protein 27 4.2
At1g20640 unknown protein 27 4.2
At1g04400 cryptochrome 2 apoprotein like protein 27 4.2
At5g51300 unknown protein 26 5.5
>At4g21720 putative protein
Length = 139
Score = 191 bits (486), Expect = 8e-50
Identities = 82/139 (58%), Positives = 105/139 (74%), Gaps = 4/139 (2%)
Query: 1 MMPQQWASPCGNQCTHKYAALTQIPWRVFCKKGCNSDGETWEECLEECDHICYKDPVLKD 60
M+PQQW PCG++CT+KY+A TQ+PWRVFCKKGC++D ++WE+C+ +C ICYKDPVLKD
Sbjct: 1 MIPQQWTPPCGSKCTNKYSAFTQLPWRVFCKKGCDADSDSWEDCVGDCSEICYKDPVLKD 60
Query: 61 QKWSAYIDRSPGAASYSEECFHACVSGCGYKFEVQQEKAEKICPNRPLKPAPVQKPKPQP 120
+ W+A IDRSPG A YSEECFHACV+GCGYKF+V+ E K+ P RP P P +P P P
Sbjct: 61 RPWTAVIDRSPGDAKYSEECFHACVAGCGYKFDVEAEIVNKVKPKRPPPPPPKPQPPPPP 120
Query: 121 ---VEQLDPP-EDMHGISA 135
+ + PP ED+ SA
Sbjct: 121 PRSQKPMQPPTEDVLATSA 139
>At5g25560 unknown protein
Length = 303
Score = 30.8 bits (68), Expect = 0.23
Identities = 21/74 (28%), Positives = 29/74 (38%), Gaps = 10/74 (13%)
Query: 42 EECLEEC-DHICYKDPVLKD---------QKWSAYIDRSPGAASYSEECFHACVSGCGYK 91
++CLEE DH Y P+ +K+ I +P Y + CG K
Sbjct: 219 QKCLEEMRDHYQYACPLCSKSVCDMSKVWEKFDMEIAATPMPEPYQNRMVQILCNDCGKK 278
Query: 92 FEVQQEKAEKICPN 105
EVQ + CPN
Sbjct: 279 AEVQYHVVAQKCPN 292
>At3g12000 hypothetical protein
Length = 439
Score = 30.8 bits (68), Expect = 0.23
Identities = 26/99 (26%), Positives = 44/99 (44%), Gaps = 29/99 (29%)
Query: 38 GETWEECLEECDHICYKDP---VLKDQKW----SAYIDRSPGAASYSEECFHACV----- 85
G+T+E C+ + C++D ++K K +A +D++ G E C C
Sbjct: 342 GDTFEGCVRKSRLNCHRDGFFLLMKRMKLPGTSTAIVDKTIGLNECKERCSKDCNCTGFA 401
Query: 86 --------SGC--------GYKFEVQQEKAEKICPNRPL 108
SGC GY+ E+++ ++ IC NRPL
Sbjct: 402 NKDIQNGGSGCVIWTGGAHGYE-ELRRRRSRSICQNRPL 439
>At3g19020 hypothetical protein
Length = 951
Score = 30.4 bits (67), Expect = 0.29
Identities = 13/32 (40%), Positives = 19/32 (58%)
Query: 97 EKAEKICPNRPLKPAPVQKPKPQPVEQLDPPE 128
E++ K P + P P + PKPQP +Q PP+
Sbjct: 558 EESPKPQPPKQETPKPEESPKPQPPKQEQPPK 589
Score = 27.7 bits (60), Expect = 1.9
Identities = 14/34 (41%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
Query: 97 EKAEKICPNRPLKPAPVQKPKPQPVEQLDP-PED 129
E++ K P + P P + PKPQP +Q P PE+
Sbjct: 526 EESPKPQPPKQETPKPEESPKPQPPKQETPKPEE 559
Score = 27.7 bits (60), Expect = 1.9
Identities = 14/34 (41%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
Query: 97 EKAEKICPNRPLKPAPVQKPKPQPVEQLDP-PED 129
E++ K P + P P + PKPQP +Q P PE+
Sbjct: 542 EESPKPQPPKQETPKPEESPKPQPPKQETPKPEE 575
Score = 26.9 bits (58), Expect = 3.3
Identities = 12/25 (48%), Positives = 16/25 (64%), Gaps = 1/25 (4%)
Query: 106 RPLKPAPVQKPKPQPVEQLDP-PED 129
+P P P + PKPQP +Q P PE+
Sbjct: 519 KPEPPKPEESPKPQPPKQETPKPEE 543
Score = 26.2 bits (56), Expect = 5.5
Identities = 10/21 (47%), Positives = 11/21 (51%)
Query: 107 PLKPAPVQKPKPQPVEQLDPP 127
P PAP+ P P PV PP
Sbjct: 737 PPPPAPIYSPPPPPVHSPPPP 757
>At4g38770 extensin - like protein
Length = 448
Score = 28.9 bits (63), Expect = 0.86
Identities = 13/38 (34%), Positives = 19/38 (49%)
Query: 94 VQQEKAEKICPNRPLKPAPVQKPKPQPVEQLDPPEDMH 131
V + +K CP + + P PV KP P + PP+ H
Sbjct: 311 VHKPPTKKPCPPKKVDPPPVPVHKPPPKIVIPPPKIEH 348
Score = 26.9 bits (58), Expect = 3.3
Identities = 14/33 (42%), Positives = 20/33 (60%), Gaps = 5/33 (15%)
Query: 100 EKICPNRPL--KPAPVQKP---KPQPVEQLDPP 127
+K CP + + P PV KP KP P +++DPP
Sbjct: 296 KKPCPPKKVDPPPVPVHKPPTKKPCPPKKVDPP 328
>At3g51620 unknown protein
Length = 341
Score = 28.9 bits (63), Expect = 0.86
Identities = 14/26 (53%), Positives = 17/26 (64%), Gaps = 1/26 (3%)
Query: 105 NRPLKPAPVQKP-KPQPVEQLDPPED 129
N P + AP +P +P PVE L PPED
Sbjct: 279 NHPYEKAPDFRPTEPLPVEVLSPPED 304
>At4g12470 pEARLI 1-like protein
Length = 161
Score = 28.1 bits (61), Expect = 1.5
Identities = 11/19 (57%), Positives = 13/19 (67%)
Query: 109 KPAPVQKPKPQPVEQLDPP 127
KP PV PKP+PV+ PP
Sbjct: 35 KPKPVPSPKPKPVQCPPPP 53
>At5g10550 bromodomain protein - like
Length = 678
Score = 27.7 bits (60), Expect = 1.9
Identities = 10/22 (45%), Positives = 14/22 (63%)
Query: 104 PNRPLKPAPVQKPKPQPVEQLD 125
P P++P P P PQPV Q++
Sbjct: 424 PPSPVQPPPPPSPPPQPVNQVE 445
>At3g54460 RING finger -like protein
Length = 1378
Score = 27.7 bits (60), Expect = 1.9
Identities = 18/50 (36%), Positives = 26/50 (52%), Gaps = 8/50 (16%)
Query: 77 SEECFHACVSGCGYKFEVQQEKAEKICPNRPLKPAPVQKPKPQPVEQLDP 126
SE C +SGCGY +E+Q + RP P P + P P+ + +L P
Sbjct: 1076 SERC---TISGCGYLYEMQTPETLA----RPENPNP-KWPVPKDLIELQP 1117
>At5g44680 unknown protein
Length = 353
Score = 27.3 bits (59), Expect = 2.5
Identities = 10/27 (37%), Positives = 15/27 (55%)
Query: 101 KICPNRPLKPAPVQKPKPQPVEQLDPP 127
K P +PL P + P P+P+ + PP
Sbjct: 40 KKSPPKPLNPIASKIPSPRPISLISPP 66
>At5g25590 unknown protein (At5g25590)
Length = 775
Score = 27.3 bits (59), Expect = 2.5
Identities = 14/34 (41%), Positives = 18/34 (52%), Gaps = 1/34 (2%)
Query: 94 VQQEKAEKICPNRPLKPAPVQKPKPQPVEQLDPP 127
+ Q+ EK N + PA Q P P P+E L PP
Sbjct: 70 LDQQHYEKQSRNN-VDPASPQPPPPPPIENLPPP 102
>At2g24960 hypothetical protein
Length = 797
Score = 27.3 bits (59), Expect = 2.5
Identities = 17/63 (26%), Positives = 26/63 (40%), Gaps = 8/63 (12%)
Query: 35 NSDGETWEECLEECDHICYKDPVLKDQKWSAYIDRSPGAASYSEECFHACVSGCGYKFEV 94
N DG TW+ + + V +D+ W AYI P A Y + + + C +
Sbjct: 604 NLDGFTWD--------VEKQTIVAEDEYWEAYIKEHPDATIYKGKTLDSYGNLCKLNEHL 655
Query: 95 QQE 97
QE
Sbjct: 656 SQE 658
>At5g35200 unknown protein
Length = 544
Score = 26.9 bits (58), Expect = 3.3
Identities = 15/63 (23%), Positives = 27/63 (42%)
Query: 61 QKWSAYIDRSPGAASYSEECFHACVSGCGYKFEVQQEKAEKICPNRPLKPAPVQKPKPQP 120
Q Y+ +P AA +E ++ ++ E K+ +P P PV+ +P
Sbjct: 294 QAMEEYVKEAPLAAGVKKEQVVEKLTAPKEILAIEYEIPPKVVEEKPASPEPVKAEAEKP 353
Query: 121 VEQ 123
VE+
Sbjct: 354 VEK 356
>At3g14330 hypothetical protein
Length = 710
Score = 26.9 bits (58), Expect = 3.3
Identities = 12/27 (44%), Positives = 17/27 (62%), Gaps = 1/27 (3%)
Query: 3 PQQWASPCGNQCTHKYAALTQIPWRVF 29
PQ + +P G+Q + +A QIPWR F
Sbjct: 72 PQAYLTPLGSQSIN-HAKFLQIPWRQF 97
>At2g45820 remorin
Length = 190
Score = 26.9 bits (58), Expect = 3.3
Identities = 11/31 (35%), Positives = 17/31 (54%)
Query: 95 QQEKAEKICPNRPLKPAPVQKPKPQPVEQLD 125
+++K K+ P AP ++P P PVE D
Sbjct: 3 EEQKTSKVDVESPAVLAPAKEPTPAPVEVAD 33
>At1g20135 anter-specific proline-rich protein APG precursor
Length = 534
Score = 26.9 bits (58), Expect = 3.3
Identities = 11/25 (44%), Positives = 12/25 (48%)
Query: 103 CPNRPLKPAPVQKPKPQPVEQLDPP 127
CP+ P KP P P P P PP
Sbjct: 88 CPSPPPKPQPKPPPAPSPSPCPSPP 112
>At5g09570 unknown protein
Length = 139
Score = 26.6 bits (57), Expect = 4.2
Identities = 10/22 (45%), Positives = 13/22 (58%)
Query: 106 RPLKPAPVQKPKPQPVEQLDPP 127
RP +PA + P PQ V + PP
Sbjct: 13 RPSRPAAARSPPPQSVNRAPPP 34
>At1g20640 unknown protein
Length = 844
Score = 26.6 bits (57), Expect = 4.2
Identities = 15/48 (31%), Positives = 23/48 (47%), Gaps = 2/48 (4%)
Query: 15 THKYA-ALTQIPWRVFCKKGCNSDGETWEECLEECDHICY-KDPVLKD 60
THK A T + + K GC + E + C+ D CY DP +++
Sbjct: 299 THKLPLAQTWVSCQQQNKSGCRHNDENYIHCVSTIDDACYVGDPTVRE 346
>At1g04400 cryptochrome 2 apoprotein like protein
Length = 612
Score = 26.6 bits (57), Expect = 4.2
Identities = 9/24 (37%), Positives = 15/24 (62%), Gaps = 2/24 (8%)
Query: 25 PWRVFCKKG--CNSDGETWEECLE 46
PW ++C+KG S W++CL+
Sbjct: 137 PWEIYCEKGKPFTSFNSYWKKCLD 160
>At5g51300 unknown protein
Length = 804
Score = 26.2 bits (56), Expect = 5.5
Identities = 16/41 (39%), Positives = 20/41 (48%), Gaps = 1/41 (2%)
Query: 89 GYKFEVQQEKAEKICPNRPLKPAPVQKPKPQPVEQLDPPED 129
GY+FE + A +I P AP P PQP Q PP +
Sbjct: 543 GYRFE-GRTLAVRIAGKSPPPIAPPGPPAPQPPTQGYPPSN 582
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.317 0.134 0.462
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,940,167
Number of Sequences: 26719
Number of extensions: 173688
Number of successful extensions: 839
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 26
Number of HSP's successfully gapped in prelim test: 19
Number of HSP's that attempted gapping in prelim test: 726
Number of HSP's gapped (non-prelim): 130
length of query: 135
length of database: 11,318,596
effective HSP length: 88
effective length of query: 47
effective length of database: 8,967,324
effective search space: 421464228
effective search space used: 421464228
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)
Lotus: description of TM0336.16