BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0336.10
(114 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g60600 unknown protein 77 2e-15
At1g08820 unknown protein 74 2e-14
At5g47180 VAMP (vesicle-associated membrane protein)-associated ... 73 2e-14
At2g45140 putative VAMP-associated protein 73 3e-14
At2g23830 unknown protein 63 3e-11
At1g51270 unknown protein 62 6e-11
At4g00175 putative proline-rich protein 61 8e-11
At5g63930 receptor-like protein kinase 26 2.8
At3g25020 disease resistance protein, putative 25 4.8
At3g48060 putative protein 25 6.3
At3g48050 putative protein 25 6.3
At3g02740 aspartyl protease - like predicted GPI-anchored protein 25 6.3
At5g56040 receptor protein kinase-like protein 25 8.2
>At3g60600 unknown protein
Length = 256
Score = 76.6 bits (187), Expect = 2e-15
Identities = 42/124 (33%), Positives = 74/124 (58%), Gaps = 12/124 (9%)
Query: 1 MDHAEILQIEPAELTFLTE--KKDFCKLHLINKTTNYVAFRLRLSKPEKYGVTPREGGII 58
M ++E+L +EP +L F E K+ C L+L NKT N VAF+++ + P+KY V P G++
Sbjct: 17 MSNSELLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNT-GVV 75
Query: 59 MPQGTYDIIVSRKSDKLPPPGFECTDRFFIQSMALS--------VKDLFTENRGKRHVEE 110
+P+ T +++V+ ++ K P +C D+F +Q + S ++F++ G R VEE
Sbjct: 76 LPRSTCEVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHR-VEE 134
Query: 111 KELK 114
+L+
Sbjct: 135 TKLR 138
>At1g08820 unknown protein
Length = 386
Score = 73.6 bits (179), Expect = 2e-14
Identities = 42/119 (35%), Positives = 68/119 (56%), Gaps = 12/119 (10%)
Query: 6 ILQIEPAELTFLTE--KKDFCKLHLINKTTNYVAFRLRLSKPEKYGVTPREGGIIMPQGT 63
+L I+P L F + K+ C + L N T +YVAF+++ + P+KY V P G ++ P+ T
Sbjct: 5 LLDIQPRTLQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVG-VVAPKST 63
Query: 64 YDIIVSRKSDKLPPPGFECTDRFFIQSMALSVK--------DLFTENRGKRHVEEKELK 114
+ V ++ K PPP C D+F IQS A+S + +F++ GK H+EE +L+
Sbjct: 64 CEFTVIMQAFKEPPPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGK-HIEENKLR 121
>At5g47180 VAMP (vesicle-associated membrane protein)-associated
protein-like
Length = 220
Score = 73.2 bits (178), Expect = 2e-14
Identities = 42/117 (35%), Positives = 67/117 (56%), Gaps = 11/117 (9%)
Query: 5 EILQIEPAELTFL--TEKKDFCKLHLINKTTNYVAFRLRLSKPEKYGVTPREGGIIMPQG 62
+++ I+P EL FL EK+ +C L + NKT NYVAF+++ + P+KY V P G+I P
Sbjct: 8 QLISIQPDELKFLFELEKQSYCDLKVANKTENYVAFKVKTTSPKKYFVRPNT-GVIQPWD 66
Query: 63 TYDIIVSRKSDKLPPPGFECTDRFFIQSMALS--------VKDLFTENRGKRHVEEK 111
+ I V+ ++ + PP +C D+F +QS + +D FT++ GK E K
Sbjct: 67 SCIIRVTLQAQREYPPDMQCKDKFLLQSTIVPPHTDVDELPQDTFTKDSGKTLTECK 123
>At2g45140 putative VAMP-associated protein
Length = 239
Score = 72.8 bits (177), Expect = 3e-14
Identities = 41/120 (34%), Positives = 71/120 (59%), Gaps = 12/120 (10%)
Query: 5 EILQIEPAELTFLTE--KKDFCKLHLINKTTNYVAFRLRLSKPEKYGVTPREGGIIMPQG 62
E+L I+P +L F E K+ C L+L NKT NYVAF+++ + P+KY V P G++ P+
Sbjct: 4 ELLTIDPVDLQFPFELKKQISCSLYLGNKTDNYVAFKVKTTNPKKYCVRPNT-GVVHPRS 62
Query: 63 TYDIIVSRKSDKLPPPGFECTDRFFIQSMALS--------VKDLFTENRGKRHVEEKELK 114
+ +++V+ ++ K P +C D+F +Q + S ++F++ G R VEE +L+
Sbjct: 63 SSEVLVTMQAQKEAPADLQCKDKFLLQCVVASPGATPKDVTHEMFSKEAGHR-VEETKLR 121
>At2g23830 unknown protein
Length = 149
Score = 62.8 bits (151), Expect = 3e-11
Identities = 41/124 (33%), Positives = 69/124 (55%), Gaps = 12/124 (9%)
Query: 1 MDHAEILQIEPAELTFLTE--KKDFCKLHLINKTTNYVAFRLRLSKPEKYGVTPREGGII 58
M + E+L+IEP L F E K+ C L+L NKT N VAF+++ + Y V P G+I
Sbjct: 1 MSNNELLEIEPMYLQFPFELKKQMSCSLYLTNKTDNNVAFKVKTTNRNNYCVRPNY-GLI 59
Query: 59 MPQGTYDIIVSRKSDKLPPPGFECTDRFFIQSMALS--------VKDLFTENRGKRHVEE 110
+P+ T ++V+ ++ K P + ++F IQS+ S +++F++ G VEE
Sbjct: 60 LPKSTCKVLVTMQAQKEVPSDMQSFEKFMIQSVLASPGVTAKEVTREMFSKESG-HVVEE 118
Query: 111 KELK 114
+L+
Sbjct: 119 TKLR 122
>At1g51270 unknown protein
Length = 289
Score = 61.6 bits (148), Expect = 6e-11
Identities = 39/119 (32%), Positives = 64/119 (53%), Gaps = 10/119 (8%)
Query: 5 EILQIEPAELTFLTE--KKDFCKLHLINKTTNYVAFRLRLSKPEKYGVTPREGGIIMPQG 62
E+L I+P ++ F E KK C L+L NKT NYVAF+ + + +KY V P G+++P+
Sbjct: 127 ELLIIDPVDVQFPIELNKKVSCSLNLTNKTENYVAFKAKTTNAKKYYVRPNV-GVVLPRS 185
Query: 63 TYDIIVSRKSDKLPPPGFECTDRFFIQSMAL----SVKDLFTENRGK---RHVEEKELK 114
+ +++V ++ K P +C D+ Q + + KD+ +E K EE LK
Sbjct: 186 SCEVLVIMQALKEAPADMQCRDKLLFQCKVVEPETTAKDVTSEMFSKEAGHPAEETRLK 244
Score = 26.6 bits (57), Expect = 2.2
Identities = 15/67 (22%), Positives = 32/67 (47%), Gaps = 9/67 (13%)
Query: 56 GIIMPQGTYDIIVSRKSDKLPPPGFECTDRFFIQSMALS--------VKDLFTENRGKRH 107
G+++P+ + +++V ++ K P +C D+ Q + ++F++ G R
Sbjct: 9 GVVLPRSSCEVLVVMQALKEAPADRQCKDKLLFQCKVVEPGTMDKEVTSEMFSKEAGHR- 67
Query: 108 VEEKELK 114
VEE K
Sbjct: 68 VEETIFK 74
>At4g00175 putative proline-rich protein
Length = 239
Score = 61.2 bits (147), Expect = 8e-11
Identities = 37/125 (29%), Positives = 69/125 (54%), Gaps = 13/125 (10%)
Query: 1 MDHAEILQIEPAELTFLTE--KKDFCKLHLINKTTNY-VAFRLRLSKPEKYGVTPREGGI 57
M +++ I P EL F E K+ C + L NKTT VAF+++ + P KY V P G +
Sbjct: 1 MTTGDLVNIHPTELKFPFELKKQSSCSMQLTNKTTTQCVAFKVKTTNPRKYCVRPNTG-V 59
Query: 58 IMPQGTYDIIVSRKSDKLPPPGFECTDRFFIQSMALS--------VKDLFTENRGKRHVE 109
++P + ++ V+ ++ K P +C D+F +Q++ +S + ++F + G R +E
Sbjct: 60 VLPGDSCNVTVTMQAQKEAPLDMQCKDKFLVQTVVVSDGTTSKEVLAEMFNKEAG-RVIE 118
Query: 110 EKELK 114
+ +L+
Sbjct: 119 DFKLR 123
>At5g63930 receptor-like protein kinase
Length = 1102
Score = 26.2 bits (56), Expect = 2.8
Identities = 14/44 (31%), Positives = 24/44 (53%)
Query: 39 RLRLSKPEKYGVTPREGGIIMPQGTYDIIVSRKSDKLPPPGFEC 82
RL+L+ G PRE G++ GT +I ++ + ++P F C
Sbjct: 509 RLQLADNGFTGELPREIGMLSQLGTLNISSNKLTGEVPSEIFNC 552
>At3g25020 disease resistance protein, putative
Length = 890
Score = 25.4 bits (54), Expect = 4.8
Identities = 16/60 (26%), Positives = 27/60 (44%)
Query: 40 LRLSKPEKYGVTPREGGIIMPQGTYDIIVSRKSDKLPPPGFECTDRFFIQSMALSVKDLF 99
L+L K G P + + P ++D+ +R + KLP C+ F+ +KD F
Sbjct: 533 LKLRKNNLEGSIPDKYYVDTPLRSFDVGYNRLTGKLPRSLINCSALQFLSVDHNGIKDTF 592
>At3g48060 putative protein
Length = 1611
Score = 25.0 bits (53), Expect = 6.3
Identities = 18/66 (27%), Positives = 26/66 (39%), Gaps = 2/66 (3%)
Query: 42 LSKPEKYGVTPREGGIIMPQGTYDIIVSRKSDKLPPPGFECTDRFFIQSMALSVKDLFTE 101
L P K PR G+ +P G + R D + TD+ +I L V L +
Sbjct: 125 LLHPCKVAFLPR--GVELPSGISSFVCRRVYDVTNERLWWLTDQDYIDDRQLEVDKLLCK 182
Query: 102 NRGKRH 107
R + H
Sbjct: 183 TRSEMH 188
>At3g48050 putative protein
Length = 1613
Score = 25.0 bits (53), Expect = 6.3
Identities = 18/66 (27%), Positives = 26/66 (39%), Gaps = 2/66 (3%)
Query: 42 LSKPEKYGVTPREGGIIMPQGTYDIIVSRKSDKLPPPGFECTDRFFIQSMALSVKDLFTE 101
L P K PR G+ +P G + R D + TD+ +I L V L +
Sbjct: 125 LLHPCKVAFLPR--GVELPSGISSFVCRRVYDVTNERLWWLTDQDYIDDRQLEVDKLLCK 182
Query: 102 NRGKRH 107
R + H
Sbjct: 183 TRSEMH 188
>At3g02740 aspartyl protease - like predicted GPI-anchored protein
Length = 488
Score = 25.0 bits (53), Expect = 6.3
Identities = 16/50 (32%), Positives = 21/50 (42%), Gaps = 1/50 (2%)
Query: 5 EILQIEPAELTFLTEKKDFCKLHLINKTTNYVAFRLRLSKPEKYGVTPRE 54
EIL P ELT T ++ F H +K + + K V PRE
Sbjct: 335 EILASHP-ELTLHTVQESFTCFHYTDKLDRFPTVTFQFDKSVSLAVYPRE 383
>At5g56040 receptor protein kinase-like protein
Length = 1090
Score = 24.6 bits (52), Expect = 8.2
Identities = 16/51 (31%), Positives = 22/51 (42%)
Query: 38 FRLRLSKPEKYGVTPREGGIIMPQGTYDIIVSRKSDKLPPPGFECTDRFFI 88
+RLRL+ G P E G + DI +R +PP CT F+
Sbjct: 460 YRLRLNGNRLAGNIPAEIGNLKNLNFIDISENRLIGNIPPEISGCTSLEFV 510
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.320 0.139 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,733,212
Number of Sequences: 26719
Number of extensions: 108579
Number of successful extensions: 213
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 192
Number of HSP's gapped (non-prelim): 14
length of query: 114
length of database: 11,318,596
effective HSP length: 90
effective length of query: 24
effective length of database: 8,913,886
effective search space: 213933264
effective search space used: 213933264
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)
Lotus: description of TM0336.10