BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0335a.6
(109 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g03530 putative reverse transcriptase 78 6e-16
At2g11140 putative retroelement pol polyprotein 75 4e-15
At2g20460 putative retroelement pol polyprotein 74 2e-14
At2g16000 putative retroelement pol polyprotein 74 2e-14
At1g47650 hypothetical protein 72 3e-14
At4g09480 unknown protein 69 4e-13
At4g17450 retrotransposon like protein 68 7e-13
At4g14460 retrovirus-related like polyprotein 68 7e-13
At1g70010 hypothetical protein 67 1e-12
At4g10980 putative protein 64 1e-11
At1g43940 hypothetical protein 60 2e-10
At2g23330 putative retroelement pol polyprotein 59 4e-10
At2g07010 putative retroelement pol polyprotein 58 9e-10
At2g15920 putative retroelement pol polyprotein 57 2e-09
At1g36620 hypothetical protein 56 3e-09
At2g13940 putative retroelement pol polyprotein 56 3e-09
At4g22040 LTR retrotransposon like protein 55 6e-09
At1g57640 55 6e-09
At1g26990 polyprotein, putative 55 8e-09
At1g21220 hypothetical protein 51 1e-07
>At4g03530 putative reverse transcriptase
Length = 374
Score = 78.2 bits (191), Expect = 6e-16
Identities = 41/91 (45%), Positives = 60/91 (65%), Gaps = 3/91 (3%)
Query: 18 PRLVSQP--LTESNYNTWSRAVLVALSMKNKVPFVDGSLPRPEEDNPQLNSWIRNNNVVI 75
P LV P L ++Y TW A+ ++ KNK+ FVDGS+P+P++D+P W R N++V
Sbjct: 78 PGLVLAPELLDGNSYGTWIIAMTTSIEAKNKLGFVDGSIPKPDDDDPYCKIWRRCNSMVK 137
Query: 76 SWIFNAVSKDITTS-MYSDTASEIYHGLDTR 105
SW+ N+VSK+I TS +Y TA+ I+ L TR
Sbjct: 138 SWLLNSVSKEIYTSILYFPTAAAIWKDLYTR 168
>At2g11140 putative retroelement pol polyprotein
Length = 411
Score = 75.5 bits (184), Expect = 4e-15
Identities = 41/91 (45%), Positives = 57/91 (62%), Gaps = 1/91 (1%)
Query: 16 PRPRLVSQPLTESNYNTWSRAVLVALSMKNKVPFVDGSLPRPEEDNPQLNSWIRNNNVVI 75
P +V+ L SNYN+WS A+ ++L KNK+ FVDGSL RP D+ W R N++V
Sbjct: 75 PGLSIVAHVLDGSNYNSWSIAMRISLDAKNKLGFVDGSLLRPSVDDSTFRIWSRCNSMVK 134
Query: 76 SWIFNAVSKDITTS-MYSDTASEIYHGLDTR 105
SWI N V+K+I S +Y + A E++ L TR
Sbjct: 135 SWILNVVNKEIYDSILYYEDAVEMWTDLFTR 165
>At2g20460 putative retroelement pol polyprotein
Length = 1461
Score = 73.6 bits (179), Expect = 2e-14
Identities = 39/95 (41%), Positives = 57/95 (59%), Gaps = 1/95 (1%)
Query: 16 PRPRLVSQPLTESNYNTWSRAVLVALSMKNKVPFVDGSLPRPEEDNPQLNSWIRNNNVVI 75
P ++S L E+ Y WS A+ ++L KNK+ FVDGSLPRP E +P W R N++V
Sbjct: 74 PGLSIISHRLDETTYGDWSVAMRISLDAKNKLGFVDGSLPRPLESDPNFRLWSRCNSMVK 133
Query: 76 SWIFNAVSKDITTSMYS-DTASEIYHGLDTRRSTT 109
SW+ N+VS I S+ + A++I+ L R + T
Sbjct: 134 SWLLNSVSPQIYRSILRLNDATDIWRDLFDRFNLT 168
>At2g16000 putative retroelement pol polyprotein
Length = 1454
Score = 73.6 bits (179), Expect = 2e-14
Identities = 38/95 (40%), Positives = 58/95 (61%), Gaps = 1/95 (1%)
Query: 16 PRPRLVSQPLTESNYNTWSRAVLVALSMKNKVPFVDGSLPRPEEDNPQLNSWIRNNNVVI 75
P ++S L E+NY WS A+L++L KNK F+DG+L RP E + W R N++V
Sbjct: 70 PGLNIISHRLDETNYGDWSVAMLISLDAKNKTGFIDGTLSRPLESDLNFRLWSRCNSMVK 129
Query: 76 SWIFNAVSKDITTS-MYSDTASEIYHGLDTRRSTT 109
SW+ N+VS I S + + AS+I+ L++R + T
Sbjct: 130 SWLLNSVSPQIYRSILRMNDASDIWRDLNSRFNVT 164
>At1g47650 hypothetical protein
Length = 1409
Score = 72.4 bits (176), Expect = 3e-14
Identities = 37/95 (38%), Positives = 57/95 (59%), Gaps = 1/95 (1%)
Query: 16 PRPRLVSQPLTESNYNTWSRAVLVALSMKNKVPFVDGSLPRPEEDNPQLNSWIRNNNVVI 75
P ++S L E+NY W+ A+L++L KNK F+DG+LPRP E + W R ++V
Sbjct: 73 PGLNIISHRLDETNYGDWNVAMLISLDAKNKSGFIDGTLPRPLESDKNFRLWSRCKSMVK 132
Query: 76 SWIFNAVSKDITTS-MYSDTASEIYHGLDTRRSTT 109
SW+ N+VS I S + + AS+I+ L +R + T
Sbjct: 133 SWLLNSVSPQIYRSILRMNDASDIWRDLHSRFNVT 167
>At4g09480 unknown protein
Length = 301
Score = 68.9 bits (167), Expect = 4e-13
Identities = 35/99 (35%), Positives = 62/99 (62%), Gaps = 1/99 (1%)
Query: 8 FLPSSFRWPRPRLVSQPLTESNYNTWSRAVLVALSMKNKVPFVDGSLPRPEEDNPQLNSW 67
FL SS + + +P + S +++W R+V +AL+++NK+ F+DG++P+P + SW
Sbjct: 27 FLHSSDQAGLILVSDRPSSGSEFHSWRRSVRMALNVRNKLGFIDGTIPQPPSTHRDAGSW 86
Query: 68 IRNNNVVISWIFNAVSKDITTS-MYSDTASEIYHGLDTR 105
R N++V +W+ N+VSK I S ++ TA I+ L +R
Sbjct: 87 SRCNDMVATWLMNSVSKKIGQSLLFMSTAESIWKNLLSR 125
>At4g17450 retrotransposon like protein
Length = 1433
Score = 68.2 bits (165), Expect = 7e-13
Identities = 39/93 (41%), Positives = 53/93 (56%), Gaps = 6/93 (6%)
Query: 4 HCQSFLPSSFRWPRPRLVSQPLTESNYNTWSRAVLVALSMKNKVPFVDGSLPRPEEDNPQ 63
H FL SS P +VS L +NYN WS A+ ++L KNK+ FVDGSLPRP+ +
Sbjct: 65 HSPYFLHSSDH-PGLNIVSHILDGTNYNNWSIAMRMSLDAKNKLSFVDGSLPRPDVSDRM 123
Query: 64 LNSWIRNNNVVISWIFNAVSKDITTSMYSDTAS 96
W R N++V +W+ N V T M++D S
Sbjct: 124 FKIWSRCNSMVKTWLLNVV-----TEMWNDLFS 151
>At4g14460 retrovirus-related like polyprotein
Length = 1489
Score = 68.2 bits (165), Expect = 7e-13
Identities = 33/88 (37%), Positives = 60/88 (67%), Gaps = 2/88 (2%)
Query: 20 LVSQPLTE-SNYNTWSRAVLVALSMKNKVPFVDGSLPRPEEDNPQLNSWIRNNNVVISWI 78
LVS LT S++++W R++L+AL+++NK+ F++G++ +P ED+ +W R N++V +W+
Sbjct: 46 LVSDRLTTASDFHSWRRSILMALNVRNKLGFINGTITKPPEDHRDFGAWSRCNDIVSTWL 105
Query: 79 FNAVSKDITTS-MYSDTASEIYHGLDTR 105
N+V K I S +Y T I++ L +R
Sbjct: 106 MNSVDKKIGQSLLYIATVQGIWNNLLSR 133
>At1g70010 hypothetical protein
Length = 1315
Score = 67.0 bits (162), Expect = 1e-12
Identities = 32/67 (47%), Positives = 48/67 (70%), Gaps = 1/67 (1%)
Query: 40 ALSMKNKVPFVDGSLPRPEEDNPQLNSWIRNNNVVISWIFNAVSKDITTS-MYSDTASEI 98
++ KNK+ FVDGS+P+P++D+P W R N++V SW+ N+VSK+I TS +Y TA+ I
Sbjct: 4 SIEAKNKLGFVDGSIPKPDDDDPYCKIWRRCNSMVKSWLLNSVSKEIYTSILYFPTAAAI 63
Query: 99 YHGLDTR 105
+ L TR
Sbjct: 64 WKDLYTR 70
>At4g10980 putative protein
Length = 294
Score = 63.9 bits (154), Expect = 1e-11
Identities = 32/88 (36%), Positives = 59/88 (66%), Gaps = 2/88 (2%)
Query: 20 LVSQPL-TESNYNTWSRAVLVALSMKNKVPFVDGSLPRPEEDNPQLNSWIRNNNVVISWI 78
LVS+ L T S++++W R++ +AL+++NK+ F+DG++ +P D+ +W R N+ V +W+
Sbjct: 47 LVSERLNTASDFHSWRRSIWMALNVRNKLGFIDGTIVKPPLDHRDYGAWSRCNDTVSTWL 106
Query: 79 FNAVSKDITTS-MYSDTASEIYHGLDTR 105
N+VSK I S ++ TA I+ + +R
Sbjct: 107 MNSVSKKIGQSLLFIPTAEGIWKNMLSR 134
>At1g43940 hypothetical protein
Length = 171
Score = 60.1 bits (144), Expect = 2e-10
Identities = 27/71 (38%), Positives = 43/71 (60%), Gaps = 1/71 (1%)
Query: 20 LVSQPLT-ESNYNTWSRAVLVALSMKNKVPFVDGSLPRPEEDNPQLNSWIRNNNVVISWI 78
L+SQPL NY+ WS + +AL + K F DGS+P+P++D+ W NN +V+SWI
Sbjct: 99 LISQPLLPRPNYDKWSINIRLALQAQKKFGFADGSIPKPDDDSEDYEDWCANNALVVSWI 158
Query: 79 FNAVSKDITTS 89
+ + + +S
Sbjct: 159 KLTIVETLRSS 169
>At2g23330 putative retroelement pol polyprotein
Length = 1496
Score = 58.9 bits (141), Expect = 4e-10
Identities = 31/93 (33%), Positives = 53/93 (56%), Gaps = 2/93 (2%)
Query: 16 PRPRLVSQPLTESNYNTWSRAVLVALSMKNKVPFVDGSLPRPEEDNPQLNSWIRNNNVVI 75
P + S L E+NY WS + L K K+ F+DGS+P+P D P+L+ WI N++++
Sbjct: 30 PGALISSVVLKENNYAEWSEELQNFLRAKQKLGFIDGSIPKPAAD-PELSLWIAINSMIV 88
Query: 76 SWIFNAVSKDITTSM-YSDTASEIYHGLDTRRS 107
WI ++ I +++ + AS+++ L R S
Sbjct: 89 GWIRTSIDPTIRSTVGFVSEASQLWENLRRRFS 121
>At2g07010 putative retroelement pol polyprotein
Length = 1413
Score = 57.8 bits (138), Expect = 9e-10
Identities = 25/75 (33%), Positives = 42/75 (55%)
Query: 16 PRPRLVSQPLTESNYNTWSRAVLVALSMKNKVPFVDGSLPRPEEDNPQLNSWIRNNNVVI 75
P + S LT NYN WS +L AL K K F++GS+ +P DNP +W N++++
Sbjct: 35 PGAMISSVMLTGDNYNEWSTKMLNALQAKRKTGFINGSISKPPLDNPDYENWQAVNSMIV 94
Query: 76 SWIFNAVSKDITTSM 90
WI ++ + +++
Sbjct: 95 GWIRASIEPKVKSTV 109
>At2g15920 putative retroelement pol polyprotein
Length = 1100
Score = 57.0 bits (136), Expect = 2e-09
Identities = 31/94 (32%), Positives = 52/94 (54%), Gaps = 11/94 (11%)
Query: 16 PRPRLVSQPLTESNYNTWSRAVLVALSMKNKVPFVDGSLPRPEEDNPQLNSWIRNNNVVI 75
P ++S L E+NY W+ A+L++L +KNK F+DG+LPRP E + W R N+++
Sbjct: 26 PGLNIISHRLDETNYGDWNVAMLISLDVKNKSGFIDGTLPRPLETDKNFCLWSRCNSMIC 85
Query: 76 SWIFNAVSKDITTSMYSDTASEIYHGLDTRRSTT 109
I + + AS+I+ L++R + T
Sbjct: 86 RSI-----------LRMNDASDIWRDLNSRFNMT 108
>At1g36620 hypothetical protein
Length = 1152
Score = 56.2 bits (134), Expect = 3e-09
Identities = 26/93 (27%), Positives = 47/93 (49%), Gaps = 1/93 (1%)
Query: 16 PRPRLVSQPLTESNYNTWSRAVLVALSMKNKVPFVDGSLPRPEEDNPQLNSWIRNNNVVI 75
P L L NY W++ L K K+ F+DG+L +P D+P W++ N++++
Sbjct: 33 PHHVLTPMLLNGENYERWAKLTRNNLQAKQKLGFIDGTLTKPSSDSPDYPRWLQTNSMLV 92
Query: 76 SWIFNAVSKDITTSM-YSDTASEIYHGLDTRRS 107
W++ ++ + S+ D A ++ L TR S
Sbjct: 93 GWLYASLDPQVQKSISVVDNARVMWESLRTRYS 125
>At2g13940 putative retroelement pol polyprotein
Length = 1501
Score = 55.8 bits (133), Expect = 3e-09
Identities = 25/80 (31%), Positives = 43/80 (53%), Gaps = 2/80 (2%)
Query: 16 PRPRLVSQPLTESNYNTWSRAVLVALSMKNKVPFVDGSLPRPEEDNPQLNSWIRNNNVVI 75
P + S L NYN W+ +L AL K K F++G++PRP ++P +W N++++
Sbjct: 40 PGAVISSVELNGDNYNQWATEMLNALQAKRKTGFINGTIPRPPPNDPNYENWTAVNSMIV 99
Query: 76 SWIFNAVSKDI--TTSMYSD 93
WI ++ + T + SD
Sbjct: 100 GWIRTSIEPKVKATVTFISD 119
>At4g22040 LTR retrotransposon like protein
Length = 1109
Score = 55.1 bits (131), Expect = 6e-09
Identities = 24/92 (26%), Positives = 53/92 (57%), Gaps = 2/92 (2%)
Query: 20 LVSQPLTESN-YNTWSRAVLVALSMKNKVPFVDGSLPRPEEDNPQLNSWIRNNNVVISWI 78
++S P+ ++N Y W+ AL + K F+DG++P+P + +P L W+ N +++SW+
Sbjct: 32 VISHPILKTNNYEEWACGFKTALRSRKKFGFLDGTIPQPLDGSPDLEDWLTINALLVSWM 91
Query: 79 FNAVSKDITTSM-YSDTASEIYHGLDTRRSTT 109
+ ++ T++ + D A +++ + R S +
Sbjct: 92 KMTIDSELLTNISHRDVARDLWEQIRKRFSVS 123
>At1g57640
Length = 1444
Score = 55.1 bits (131), Expect = 6e-09
Identities = 24/92 (26%), Positives = 53/92 (57%), Gaps = 2/92 (2%)
Query: 20 LVSQPLTESN-YNTWSRAVLVALSMKNKVPFVDGSLPRPEEDNPQLNSWIRNNNVVISWI 78
++S P+ ++N Y W+ AL + K F+DG++P+P + +P L W+ N +++SW+
Sbjct: 32 VISHPILKTNNYEEWACGFKTALRSRKKFGFLDGTIPQPLDGSPDLEDWLTINALLVSWM 91
Query: 79 FNAVSKDITTSM-YSDTASEIYHGLDTRRSTT 109
+ ++ T++ + D A +++ + R S +
Sbjct: 92 KMTIDSELLTNISHRDVARDLWEQIRKRFSVS 123
>At1g26990 polyprotein, putative
Length = 1436
Score = 54.7 bits (130), Expect = 8e-09
Identities = 27/59 (45%), Positives = 37/59 (61%)
Query: 16 PRPRLVSQPLTESNYNTWSRAVLVALSMKNKVPFVDGSLPRPEEDNPQLNSWIRNNNVV 74
P +V+ L SNYN+WS A+ ++L KNK+ FVDGSL RP D+ W R N++V
Sbjct: 66 PGLSIVAHILDGSNYNSWSIAMRISLDAKNKLGFVDGSLLRPSVDDSTFRIWSRCNSMV 124
>At1g21220 hypothetical protein
Length = 227
Score = 50.8 bits (120), Expect = 1e-07
Identities = 23/78 (29%), Positives = 46/78 (58%), Gaps = 1/78 (1%)
Query: 26 TESNYNTWSRAVLVALSMKNKVPFVDGSLPRPEEDNPQLNSWIRNNNVVISWIFNAVSKD 85
+E NY W ++ L K+K+ F+DG++ +PE +P W N V SW+ +++S++
Sbjct: 33 SEDNYFMWKIDFVIFLQSKSKIGFIDGTIAKPEPSSPLYRPWETCNARVKSWMMSSLSEE 92
Query: 86 ITTSM-YSDTASEIYHGL 102
+ + + +S+TA + + L
Sbjct: 93 LRSYVTFSETAHKAWEDL 110
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.316 0.130 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,654,724
Number of Sequences: 26719
Number of extensions: 102885
Number of successful extensions: 345
Number of sequences better than 10.0: 67
Number of HSP's better than 10.0 without gapping: 58
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 272
Number of HSP's gapped (non-prelim): 69
length of query: 109
length of database: 11,318,596
effective HSP length: 85
effective length of query: 24
effective length of database: 9,047,481
effective search space: 217139544
effective search space used: 217139544
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)
Lotus: description of TM0335a.6