BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0327.6
(442 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g15020 putative protein 275 4e-74
At5g64190 putative protein 138 5e-33
At2g40390 unknown protein 133 2e-31
At1g27750 unknown protein 36 0.037
At5g53870 predicted GPI-anchored protein 34 0.14
At2g31900 putative unconventional myosin 31 1.6
At4g37450 arabinogalactan protein AGP18 30 2.0
At1g61080 hypothetical protein 30 2.0
At3g20620 unknown protein 30 2.7
At1g75100 unknown protein 30 2.7
At2g46380 hypothetical protein 30 3.5
At3g60000 putative protein 29 4.5
At1g05200 putative glutamate receptor protein / ligand-gated ion... 29 4.5
At1g68790 putative nuclear matrix constituent protein 1 (NMCP1) 29 5.9
At1g26150 Pto kinase interactor, putative 29 5.9
At5g21930 metal-transporting ATPase - like protein 28 7.7
At4g03020 putative WD-repeat protein 28 7.7
At1g05530 hypothetical protein 28 7.7
>At2g15020 putative protein
Length = 526
Score = 275 bits (703), Expect = 4e-74
Identities = 127/250 (50%), Positives = 180/250 (71%), Gaps = 18/250 (7%)
Query: 197 AHHQLEAHVQLEYTVGFHDGFIQVRARVDNIRLHVARLGFNKDGDDVD------FIDEKH 250
+H Q E VQ EY+V F++ +I+V ARVDNIR+HV++LGF+K G V+ + +E++
Sbjct: 286 SHQQAEILVQFEYSVKFYENYIKVNARVDNIRIHVSKLGFHKGGVGVENQIADCYSEERY 345
Query: 251 FPSRVRVWVGPEIGAAYVGGLSLGRSTENNEREVEIQKIVKGNFEKSEHISKVKAAARSS 310
FPSRVRVW+GPE+G+++V GLSLGRST+N ER++E+ +++KGNF K + +VKA AR +
Sbjct: 346 FPSRVRVWLGPELGSSHVSGLSLGRSTKNEERDIEVTRVLKGNFGKGKVAPRVKARARMA 405
Query: 311 RRTRTKSWRMDQDAEGNAAIFDVVLHDNMTGLEVGAWKPTGQTGDDPVHGMRGRYSGASR 370
+ + K WR++Q++EGNAA+FD VL+D +G EV KP + G
Sbjct: 406 TKRKVKDWRIEQESEGNAAVFDAVLYDRESGQEVTTVKP------------KPNQEGLKN 453
Query: 371 PFNKSGSVVIAGDEYGEEVGWRLSKEMEGSVLKWRIGGEFWVSYWPNQVKGSYFETRYVE 430
F KSG +V DEYG+EVGWR+ +EMEGSVLKWR+GG+ W++YWPN++ ++ETR VE
Sbjct: 454 VFTKSGGMVFGRDEYGDEVGWRVGREMEGSVLKWRLGGKIWLTYWPNKLNTLFYETRCVE 513
Query: 431 WCDEVDLPLI 440
WCDEVDLPL+
Sbjct: 514 WCDEVDLPLL 523
>At5g64190 putative protein
Length = 502
Score = 138 bits (348), Expect = 5e-33
Identities = 81/248 (32%), Positives = 134/248 (53%), Gaps = 14/248 (5%)
Query: 199 HQLEAHVQLEYTVGFHDGFIQVRARVDNIRLHVARLGFNK------DGDDVDFIDEKHFP 252
+QLE +Q + V D +I V ++DNIR V +L K G+ EKHFP
Sbjct: 261 NQLEGVMQFNHKVVVRDNWIDVIVKIDNIRYDVIKLVNEKLMSRRGAGEH-----EKHFP 315
Query: 253 SRVRVWVGPEIGAAYVGGLSLGRSTENNEREVEIQKIVKGNFEKSEHISKVKAAARSSRR 312
SR+ + + P + ++ +S+ +S+ N RE E+++ ++G+F+ + A +S
Sbjct: 316 SRISLQLTPTLQTDFIS-VSVSKSSNNPGREFEVERSIEGSFDPPNSLGLRVAGREASTM 374
Query: 313 TRTKSWRMDQDAEGNAAIFDVVLHDNMTG-LEVGAWKPTGQTGDDPVHGMRGRYSGASRP 371
T T W+++Q G A + +L+D+ G EV + KP+ + P + RY+ A R
Sbjct: 375 TMTP-WKLEQSVLGYTANLNWILYDSSVGGREVFSTKPSRFSIMSPRSWFKDRYARAYRS 433
Query: 372 FNKSGSVVIAGDEYGEEVGWRLSKEMEGSVLKWRIGGEFWVSYWPNQVKGSYFETRYVEW 431
F + G V+ AGDEYGE V W++ K G ++W I G W++YWPN+ K Y ETR +E+
Sbjct: 434 FTRRGGVIFAGDEYGESVVWKIGKGALGGTMEWEIKGFIWLTYWPNKYKTFYHETRRLEF 493
Query: 432 CDEVDLPL 439
++L +
Sbjct: 494 TQLLNLTI 501
>At2g40390 unknown protein
Length = 496
Score = 133 bits (334), Expect = 2e-31
Identities = 76/243 (31%), Positives = 128/243 (52%), Gaps = 2/243 (0%)
Query: 198 HHQLEAHVQLEYTVGFHDGFIQVRARVDNIRLHVARLGFNKDGDDVDF-IDEKHFPSRVR 256
+HQLE +QL + + + + V +DN+R + RL K + +EKHFPSR+
Sbjct: 254 YHQLEGVIQLNHRIYVREKWFNVAVNIDNVRCDIIRLVNEKLLSERGMGTEEKHFPSRIS 313
Query: 257 VWVGPEIGAAYVGGLSLGRSTENNEREVEIQKIVKGNFEKSEHISKVKAAARSSRRTRTK 316
+ + P + + +S+ +S+EN E E++K ++ + +K +A + K
Sbjct: 314 LQLTPT-NQSNILMVSVQKSSENPLTEFEVEKGIEATIDPPNTFFGLKVSANETTTKSMK 372
Query: 317 SWRMDQDAEGNAAIFDVVLHDNMTGLEVGAWKPTGQTGDDPVHGMRGRYSGASRPFNKSG 376
W+ ++ G +A LHD G EV + KP+ + +P + RYS A RPF K G
Sbjct: 373 PWKFEEWVHGYSANLTWFLHDLDDGREVSSSKPSKVSMMNPRAWFKNRYSSAFRPFTKQG 432
Query: 377 SVVIAGDEYGEEVGWRLSKEMEGSVLKWRIGGEFWVSYWPNQVKGSYFETRYVEWCDEVD 436
VV AGD YG+ V W++ K G V+++ + G W++YWPN+ Y +TR +E+ + +
Sbjct: 433 GVVFAGDSYGQSVLWKVDKTAIGKVMEFEVKGCVWLTYWPNKHHTFYSDTRKLEFKEMLY 492
Query: 437 LPL 439
L L
Sbjct: 493 LNL 495
>At1g27750 unknown protein
Length = 1975
Score = 36.2 bits (82), Expect = 0.037
Identities = 20/54 (37%), Positives = 26/54 (48%), Gaps = 3/54 (5%)
Query: 63 PPIQHPKASLGIREMQPFSR*PKPLPSSAPPTPPGNHIPLTHRAR*HLPKIPAP 116
PP+ H S+ +QP S+ P+P P PP P PL H H P +P P
Sbjct: 849 PPLGHSLPSVLQPPLQPQSQPPEPPPEMMPPPPQALPPPLPHS---HPPLVPPP 899
>At5g53870 predicted GPI-anchored protein
Length = 370
Score = 34.3 bits (77), Expect = 0.14
Identities = 27/101 (26%), Positives = 41/101 (39%), Gaps = 13/101 (12%)
Query: 45 LRVRLRRGGDIHRVLARLPPIQHPKASLGIREMQPFSR*PK---PLPSSAPPTPPGNHIP 101
L VR + H + + P Q PK+ + + P S K P S +P PP + P
Sbjct: 128 LAVRNQPSAPAHSPVPSVSPTQPPKSHSPVSPVAPASAPSKSQPPRSSVSPAQPPKSSSP 187
Query: 102 LTH-------RAR*HLPKIPAPETEAG---THIMDHGLTHT 132
++H A H P P+P ++ +H H HT
Sbjct: 188 ISHTPALSPSHATSHSPATPSPSPKSPSPVSHSPSHSPAHT 228
>At2g31900 putative unconventional myosin
Length = 1490
Score = 30.8 bits (68), Expect = 1.6
Identities = 24/71 (33%), Positives = 39/71 (54%), Gaps = 10/71 (14%)
Query: 276 STENNEREVEIQKIVKGNFEKSEHISKVKAAARSSRRTRTKSWRMDQDAEGNAAIFDVV- 334
+++NNE EVE+ K+ KG ++ E K A SR + T++ +DA+ A F +
Sbjct: 910 NSQNNELEVEVAKL-KGKIKEFE--VKCFALENDSRASVTEA----EDAKSKAVEFQEII 962
Query: 335 --LHDNMTGLE 343
LH N++ LE
Sbjct: 963 ERLHTNLSNLE 973
>At4g37450 arabinogalactan protein AGP18
Length = 209
Score = 30.4 bits (67), Expect = 2.0
Identities = 18/58 (31%), Positives = 29/58 (49%), Gaps = 10/58 (17%)
Query: 64 PIQHPKASLGIREMQPFSR*PKPLPSSAPPTP-PGNHIPLTHRAR*HLPKIPAPETEA 120
P++ PK+ + E P P P+P S+PP P P P++ P +PAP ++
Sbjct: 66 PVESPKSPAPVSESSPP---PTPVPESSPPVPAPMVSSPVSS------PPVPAPVADS 114
>At1g61080 hypothetical protein
Length = 907
Score = 30.4 bits (67), Expect = 2.0
Identities = 22/61 (36%), Positives = 26/61 (42%), Gaps = 7/61 (11%)
Query: 63 PPIQHPKASLGIREMQPFSR*P---KPLPSSAPPTPPGNHIPLTHRAR*HLPKIPAPETE 119
PP P A + ++ P P KPL SAPP PP +P T A P P P
Sbjct: 476 PPPPLPPAVMPLKHFAPPPPTPPAFKPLKGSAPPPPPPPPLPTTIAA----PPPPPPPPR 531
Query: 120 A 120
A
Sbjct: 532 A 532
>At3g20620 unknown protein
Length = 391
Score = 30.0 bits (66), Expect = 2.7
Identities = 22/96 (22%), Positives = 42/96 (42%), Gaps = 16/96 (16%)
Query: 326 GNAAIFDVVLHDNMTGLEVGAWKPTGQTGDDPVHGMRGRYSGASRPFNKSGSVVIAGDEY 385
G++ ++ ++ + TG +WK G P+ S + RP+ ++G + + G Y
Sbjct: 193 GHSLAYECEVYSSSTG----SWKNIGTVPCLPM-------SCSLRPYRRTGHIFVGGKVY 241
Query: 386 GEEVGWRLSKEMEGSVLKWRIGGEFWVSYWPNQVKG 421
W +S + G +L + G+F V P G
Sbjct: 242 -----WLVSLDAPGKILSVDLEGKFEVINLPAYADG 272
>At1g75100 unknown protein
Length = 651
Score = 30.0 bits (66), Expect = 2.7
Identities = 27/124 (21%), Positives = 56/124 (44%), Gaps = 11/124 (8%)
Query: 279 NNEREVEIQKIVKGNFEKSEHISKVKAAARSSRRTRTKSWRMDQDAEGNAAIFDVVLHDN 338
+ E E + +KIV + K K+ A+++R + T+ R + ++G + D +
Sbjct: 339 HEEDERQDEKIVS-----EREVRKGKSKAKNTR-SFTEDSRTKKKSQGTKSSLDSSPIPD 392
Query: 339 MTGLEVGAWKPTGQTGDDPVHGMRGRYSGASRPFNKSGSVVIAGDEYGEEVGWRLSKEME 398
+ + P + G D G++G+ S + F+K SV G+ G+ WR + +
Sbjct: 393 KSSFASSSAAP--EVGKD---GVKGKVSDFVKIFSKGASVGAGGESLGQSSRWRAKETPK 447
Query: 399 GSVL 402
++
Sbjct: 448 TDII 451
>At2g46380 hypothetical protein
Length = 732
Score = 29.6 bits (65), Expect = 3.5
Identities = 17/69 (24%), Positives = 31/69 (44%), Gaps = 6/69 (8%)
Query: 275 RSTENNEREVEIQKIVKGNFEKSEHISKVKAAARSSRRTRTKSWRMDQDAEGNAAIFDVV 334
+S EN +E+E+ SE S A+ R + +T++W + D + D
Sbjct: 54 KSVENRSKEIEVTS------GSSEEESDSNASLRHQQDGQTETWDVHNDPSSKMNVVDSG 107
Query: 335 LHDNMTGLE 343
+D+ GL+
Sbjct: 108 NNDSQPGLD 116
>At3g60000 putative protein
Length = 451
Score = 29.3 bits (64), Expect = 4.5
Identities = 13/31 (41%), Positives = 16/31 (50%)
Query: 65 IQHPKASLGIREMQPFSR*PKPLPSSAPPTP 95
+ P +S +RE FSR PLP AP P
Sbjct: 206 VSSPSSSCNVRESSSFSRLGLPLPPMAPKVP 236
>At1g05200 putative glutamate receptor protein / ligand-gated ion
channel protein (AtGLR3.4)
Length = 959
Score = 29.3 bits (64), Expect = 4.5
Identities = 22/77 (28%), Positives = 36/77 (46%), Gaps = 3/77 (3%)
Query: 336 HDNMTGLEVGAWKPTGQTGDDPVHGMRGRYSGASRPFNKSGSVVIAGDEYGEEVGWRLSK 395
+DN+ EV A GD + R RY ++PF +SG VV+A + + W K
Sbjct: 552 YDNLVN-EVVADNFDVAVGDITIVTNRTRYVDFTQPFIESGLVVVAPVKEAKSSPWSFLK 610
Query: 396 EMEGSVLKWRIGGEFWV 412
++ W + G F++
Sbjct: 611 PF--TIEMWAVTGGFFL 625
>At1g68790 putative nuclear matrix constituent protein 1 (NMCP1)
Length = 1085
Score = 28.9 bits (63), Expect = 5.9
Identities = 28/125 (22%), Positives = 48/125 (38%), Gaps = 8/125 (6%)
Query: 275 RSTENNEREVEIQKIVKGNFEKSEHISKVKAAARSSRRTRTKSWRMDQDAEGNAAIFDVV 334
R N E + + KG EK E ++ S + TRT +++ + + V
Sbjct: 944 RRHRNVGTEEDKAQASKGATEKQERVNDDIRKVPSPKETRTPPEGENRENGKAEVLVETV 1003
Query: 335 LHDNMTGLEVGAWKPTGQTGDDPVHGMRGRYSGASRPFNKSGSVVIAGDEYGEEVGWRLS 394
H+ + +E TG +PV + G SG + G+E E + +
Sbjct: 1004 THEEIVTVETETVFKVNNTGKNPVEDPQLEVGG-------SGEIREHGEEDDENIS-MIE 1055
Query: 395 KEMEG 399
+E EG
Sbjct: 1056 EENEG 1060
>At1g26150 Pto kinase interactor, putative
Length = 760
Score = 28.9 bits (63), Expect = 5.9
Identities = 15/37 (40%), Positives = 19/37 (50%), Gaps = 3/37 (8%)
Query: 84 PKPLPSSAPPT---PPGNHIPLTHRAR*HLPKIPAPE 117
P LP+ PP+ PP +P +H HLP PA E
Sbjct: 154 PPSLPAPDPPSNPLPPPKLVPPSHSPPRHLPSPPASE 190
>At5g21930 metal-transporting ATPase - like protein
Length = 856
Score = 28.5 bits (62), Expect = 7.7
Identities = 13/28 (46%), Positives = 20/28 (71%)
Query: 302 KVKAAARSSRRTRTKSWRMDQDAEGNAA 329
++KA++ S T +K RM +DA+GNAA
Sbjct: 383 RIKASSTGSNSTISKIVRMVEDAQGNAA 410
>At4g03020 putative WD-repeat protein
Length = 493
Score = 28.5 bits (62), Expect = 7.7
Identities = 13/41 (31%), Positives = 22/41 (52%), Gaps = 6/41 (14%)
Query: 372 FNKSGSVVIAGDE------YGEEVGWRLSKEMEGSVLKWRI 406
F+ GS+ IAG + Y E GW++ K++ L+W +
Sbjct: 134 FSTDGSLFIAGFQGSRIRIYNVEKGWKVQKDILAKSLRWTV 174
>At1g05530 hypothetical protein
Length = 455
Score = 28.5 bits (62), Expect = 7.7
Identities = 19/59 (32%), Positives = 29/59 (48%), Gaps = 7/59 (11%)
Query: 191 HRALFHAHHQLEAHVQLEYTVGFHDGFIQVRARVDNIRLHVARLGFNKDGDDV--DFID 247
HR++ H+ +E L ++ GF DG I V N +H F ++GD DFI+
Sbjct: 45 HRSMIPNHNNVENLSFLTFSDGFDDGVISNTDDVQNRLVH-----FERNGDKALSDFIE 98
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.325 0.141 0.445
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,288,182
Number of Sequences: 26719
Number of extensions: 474913
Number of successful extensions: 1891
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 1862
Number of HSP's gapped (non-prelim): 30
length of query: 442
length of database: 11,318,596
effective HSP length: 102
effective length of query: 340
effective length of database: 8,593,258
effective search space: 2921707720
effective search space used: 2921707720
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0327.6