Lotus
BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0318.14
         (58 letters)

Database: ara_mips 
           26,719 sequences; 11,318,596 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

At3g57690 predicted GPI-anchored protein                               68  8e-13
At3g20865 unknown protein                                              42  6e-05
At4g26320 arabinogalactan-protein AGP13                                33  0.021
At5g11740 arabinogalactan-protein AGP15                                32  0.078
At5g56540 arabinogalactan-protein AGP14                                30  0.17
At5g40730 arabinogalactan-protein AGP24                                30  0.23
At1g55330 arabinogalactan-protein AGP21                                29  0.39
At4g30590 predicted GPI-anchored protein                               29  0.51
At3g13520 arabinogalactan-protein AGP12                                28  1.1
At1g03870 predicted GPI-anchored protein                               28  1.1
At5g49130 unknown protein                                              27  1.5
At4g08670 lipid transfer protein - like predicted GPI-anchored p...    27  1.5
At1g56120 receptor-like protein kinase, putative                       27  2.5
At1g17370 oligouridylate binding protein, putative                     26  3.3
At2g31850 hypothetical protein                                         26  4.3
At2g13800 receptor-like protein kinase                                 26  4.3
At3g14100 oligouridylate binding protein, putative                     25  5.6
At2g45160 putative SCARECROW gene regulator                            25  5.6
At2g26890 unknown protein                                              25  5.6
At3g55230 disease resistance response/ dirigent - like protein         25  7.3

>At3g57690 predicted GPI-anchored protein
          Length = 61

 Score = 68.2 bits (165), Expect = 8e-13
 Identities = 35/59 (59%), Positives = 45/59 (75%), Gaps = 2/59 (3%)

Query: 1  MDMKKVTCVVLIVAASMSAALATNEVPAPAPGPASGASATV--VGSLVGASVLSFFALF 57
          M+MKK+ C VL  AASM+A +A  EV APAPGPAS ASA +  +GSLVGAS++S F+ +
Sbjct: 1  MEMKKIACGVLFAAASMTAVMAAEEVGAPAPGPASAASAALPALGSLVGASLVSLFSYY 59


>At3g20865 unknown protein
          Length = 62

 Score = 42.0 bits (97), Expect = 6e-05
 Identities = 25/59 (42%), Positives = 38/59 (64%), Gaps = 3/59 (5%)

Query: 1  MDMKKVTCVVLIVAASMSA-ALATNEVPAPAPGPASGASAT--VVGSLVGASVLSFFAL 56
          M+MK +   + I A  +S+ + AT E PAP+PG +S ++    VVGS+V AS+ +F AL
Sbjct: 1  MEMKNIFVALFISAVLVSSVSAATMESPAPSPGASSASTVAFPVVGSIVAASLSAFLAL 59


>At4g26320 arabinogalactan-protein AGP13
          Length = 59

 Score = 33.5 bits (75), Expect = 0.021
 Identities = 18/48 (37%), Positives = 23/48 (47%)

Query: 10 VLIVAASMSAALATNEVPAPAPGPASGASATVVGSLVGASVLSFFALF 57
          VL+ A + SA      V APAP P S AS  +       + L+F  LF
Sbjct: 12 VLVAAMAFSAVQQAAAVEAPAPSPTSDASLAIPAFFASVATLAFGFLF 59


>At5g11740 arabinogalactan-protein AGP15
          Length = 61

 Score = 31.6 bits (70), Expect = 0.078
 Identities = 22/56 (39%), Positives = 31/56 (55%), Gaps = 3/56 (5%)

Query: 1  MDMKKVTCVVLIVAASMSAALATNEVPAPAPGPASGASATVVGSLVGASVLSFFAL 56
          M + K + VVL++      A A +E PAP+  P SG+SA +  S V A V +  AL
Sbjct: 1  MAISKASIVVLMMVIISVVASAQSEAPAPS--PTSGSSA-ISASFVSAGVAAVAAL 53


>At5g56540 arabinogalactan-protein AGP14
          Length = 60

 Score = 30.4 bits (67), Expect = 0.17
 Identities = 18/57 (31%), Positives = 26/57 (45%)

Query: 1  MDMKKVTCVVLIVAASMSAALATNEVPAPAPGPASGASATVVGSLVGASVLSFFALF 57
          M MK    V++ V A  +       V APAP P S AS+ +       +V++F   F
Sbjct: 4  MKMKLYVVVLVAVIAFSTVHQTVAAVDAPAPSPTSDASSFIPTFFASVAVMAFGFFF 60


>At5g40730 arabinogalactan-protein AGP24
          Length = 69

 Score = 30.0 bits (66), Expect = 0.23
 Identities = 19/51 (37%), Positives = 29/51 (56%), Gaps = 2/51 (3%)

Query: 6  VTCVVLIVAASMSAALATNEVPAPAPGPASGASATVVGSLVGASVLSFFAL 56
          V C++  +A   +     +  PAPAPGPAS  S+TVV +    +VL+  A+
Sbjct: 13 VVCLLATMAVVSAHEGHHHHAPAPAPGPAS--SSTVVSATNMFTVLAIAAV 61


>At1g55330 arabinogalactan-protein AGP21
          Length = 58

 Score = 29.3 bits (64), Expect = 0.39
 Identities = 16/37 (43%), Positives = 22/37 (59%), Gaps = 1/37 (2%)

Query: 5  KVTCVVLIVAASMSAALATNEVPAPAPGPASGASATV 41
          K+   +++VA + SAA A   V APAP P S A+  V
Sbjct: 7  KMMVFIMVVAVAFSAATAAT-VEAPAPSPTSDAAMFV 42



 Score = 28.5 bits (62), Expect = 0.66
 Identities = 12/31 (38%), Positives = 19/31 (60%)

Query: 1  MDMKKVTCVVLIVAASMSAALATNEVPAPAP 31
          M MK +  ++++  A  +A  AT E PAP+P
Sbjct: 4  MKMKMMVFIMVVAVAFSAATAATVEAPAPSP 34


>At4g30590 predicted GPI-anchored protein
          Length = 190

 Score = 28.9 bits (63), Expect = 0.51
 Identities = 13/28 (46%), Positives = 17/28 (60%)

Query: 14  AASMSAALATNEVPAPAPGPASGASATV 41
           A  +S    T + PAPAPGPA+  +A V
Sbjct: 143 APKVSPVSPTAQTPAPAPGPAAAHNAAV 170


>At3g13520 arabinogalactan-protein AGP12
          Length = 60

 Score = 27.7 bits (60), Expect = 1.1
 Identities = 15/57 (26%), Positives = 25/57 (43%)

Query: 1  MDMKKVTCVVLIVAASMSAALATNEVPAPAPGPASGASATVVGSLVGASVLSFFALF 57
          M MK +  +++ + A  +      +  APAP P S A+  V       + L+   LF
Sbjct: 4  MKMKLIVVLMVAIVAFSAVGNVAAQTEAPAPSPTSDAAMFVPALFASVAALASGFLF 60


>At1g03870 predicted GPI-anchored protein
          Length = 247

 Score = 27.7 bits (60), Expect = 1.1
 Identities = 13/33 (39%), Positives = 20/33 (60%)

Query: 10 VLIVAASMSAALATNEVPAPAPGPASGASATVV 42
          +L++AA + A  AT +  APAP PA   + T +
Sbjct: 11 LLLIAAVLLATKATAQPAAPAPEPAGPINLTAI 43


>At5g49130 unknown protein
          Length = 502

 Score = 27.3 bits (59), Expect = 1.5
 Identities = 16/49 (32%), Positives = 27/49 (54%), Gaps = 3/49 (6%)

Query: 5   KVTCVVLIVAASMSAALAT---NEVPAPAPGPASGASATVVGSLVGASV 50
           + T ++  +  ++SAA++T   NE+ A  P  A  A+   VG+ V  SV
Sbjct: 308 QTTSLMYTIPTALSAAVSTRVSNELGAGRPEKAKTAATVAVGAAVAVSV 356


>At4g08670 lipid transfer protein - like predicted GPI-anchored
          protein
          Length = 194

 Score = 27.3 bits (59), Expect = 1.5
 Identities = 12/25 (48%), Positives = 18/25 (72%)

Query: 22 ATNEVPAPAPGPASGASATVVGSLV 46
          A + V APAPGP+S   +TV+ S++
Sbjct: 32 AKSPVGAPAPGPSSSDCSTVIYSMM 56


>At1g56120 receptor-like protein kinase, putative
          Length = 1045

 Score = 26.6 bits (57), Expect = 2.5
 Identities = 14/44 (31%), Positives = 21/44 (46%)

Query: 12  IVAASMSAALATNEVPAPAPGPASGASATVVGSLVGASVLSFFA 55
           ++AA  +    T  V    P      + T+VG +VG  +LS FA
Sbjct: 623 LIAAVSATPDFTPTVANRPPSKGKSRTGTIVGVIVGVGLLSIFA 666


>At1g17370 oligouridylate binding protein, putative
          Length = 419

 Score = 26.2 bits (56), Expect = 3.3
 Identities = 11/22 (50%), Positives = 14/22 (63%)

Query: 19  AALATNEVPAPAPGPASGASAT 40
           A  A+N +P PAP P  G SA+
Sbjct: 339 AGTASNPLPPPAPAPIPGFSAS 360


>At2g31850 hypothetical protein
          Length = 113

 Score = 25.8 bits (55), Expect = 4.3
 Identities = 11/30 (36%), Positives = 15/30 (49%)

Query: 15  ASMSAALATNEVPAPAPGPASGASATVVGS 44
           +S  ++  +N VPAP P PA   S     S
Sbjct: 76  SSPDSSTGSNSVPAPVPSPAPAGSCCCCSS 105


>At2g13800 receptor-like protein kinase
          Length = 601

 Score = 25.8 bits (55), Expect = 4.3
 Identities = 13/29 (44%), Positives = 20/29 (68%), Gaps = 2/29 (6%)

Query: 28  APAPGPASGASATVVGSLVGASVLSFFAL 56
           +P+P P+  ++A VVG   GA++L  FAL
Sbjct: 206 SPSPSPSGTSAAIVVGVAAGAALL--FAL 232


>At3g14100 oligouridylate binding protein, putative
          Length = 427

 Score = 25.4 bits (54), Expect = 5.6
 Identities = 10/18 (55%), Positives = 12/18 (66%)

Query: 22  ATNEVPAPAPGPASGASA 39
           A+N +P PAP P  G SA
Sbjct: 347 ASNPLPPPAPAPVPGLSA 364


>At2g45160 putative SCARECROW gene regulator
          Length = 640

 Score = 25.4 bits (54), Expect = 5.6
 Identities = 12/36 (33%), Positives = 19/36 (52%)

Query: 16  SMSAALATNEVPAPAPGPASGASATVVGSLVGASVL 51
           S S++ A   VP P+PG A    + ++  L  A+ L
Sbjct: 243 SSSSSTAVAMVPVPSPGMAGDDQSVIIEQLFNAAEL 278


>At2g26890 unknown protein
          Length = 2535

 Score = 25.4 bits (54), Expect = 5.6
 Identities = 14/42 (33%), Positives = 22/42 (52%), Gaps = 2/42 (4%)

Query: 2   DMKKVTCVVLIVAASMSAALATNEVPAPAPGPASGASATVVG 43
           ++ +VT + LI     +  L  +  P P P P   A+ATV+G
Sbjct: 447 EVPEVTLMALITMLPSTPNLPVDAPPLPPPSPK--AAATVIG 486


>At3g55230 disease resistance response/ dirigent - like protein
          Length = 306

 Score = 25.0 bits (53), Expect = 7.3
 Identities = 9/23 (39%), Positives = 13/23 (56%)

Query: 20 ALATNEVPAPAPGPASGASATVV 42
          A+ T   P P PGPA+G    ++
Sbjct: 48 AVTTTPTPIPLPGPATGGPEPIL 70


  Database: ara_mips
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 2,978,382
  Number of sequences in database:  6832
  
  Database: /data/blast2/ara_mips_chr2
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 1,737,135
  Number of sequences in database:  4184
  
  Database: /data/blast2/ara_mips_chr3
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 2,236,886
  Number of sequences in database:  5377
  
  Database: /data/blast2/ara_mips_chr4
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 1,748,816
  Number of sequences in database:  4030
  
  Database: /data/blast2/ara_mips_chr5
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 2,569,679
  Number of sequences in database:  6098
  
  Database: /data/blast2/ara_mips_chl
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 25,951
  Number of sequences in database:  85
  
  Database: /data/blast2/ara_mips_mit
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 21,747
  Number of sequences in database:  113
  
Lambda     K      H
   0.319    0.128    0.353 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,024,020
Number of Sequences: 26719
Number of extensions: 26668
Number of successful extensions: 237
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 22
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 203
Number of HSP's gapped (non-prelim): 30
length of query: 58
length of database: 11,318,596
effective HSP length: 34
effective length of query: 24
effective length of database: 10,410,150
effective search space: 249843600
effective search space used: 249843600
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)


Lotus: description of TM0318.14