BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0314.16
(185 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g64080 predicted GPI-anchored protein 125 2e-29
At2g13820 predicted GPI-anchored protein 115 1e-26
At1g36150 hypothetical protein 86 1e-17
At4g08670 lipid transfer protein - like predicted GPI-anchored p... 83 7e-17
At5g09370 putative lipid transfer protein, predicted GPI-anchore... 77 4e-15
At3g22600 predicted GPI-anchored protein 69 1e-12
At2g48130 predicted GPI-anchored protein 67 7e-12
At4g14815 Expressed protein 66 9e-12
At2g44300 predicted GPI-anchored protein 62 2e-10
At3g43720 lipid-transfer protein-like, predicted GPI-anchored pr... 60 5e-10
At2g44290 non-specific lipid transfer protein (nLTP) like protein 59 2e-09
At1g55260 unknown protein 59 2e-09
At3g22611 predicted GPI-anchored protein 57 6e-09
At2g27130 predicted GPI-anchored protein 56 1e-08
At1g27950 GPI-anchored protein (LTPL) 50 5e-07
At1g05450 hypothetical protein 49 2e-06
At2g48140 unknown protein 47 8e-06
At3g58550 predicted GPI-anchored protein 45 3e-05
At3g51590 lipid transfer protein-like protein 42 2e-04
At2g38530 putative nonspecific lipid-transfer protein 42 2e-04
>At5g64080 predicted GPI-anchored protein
Length = 173
Score = 125 bits (313), Expect = 2e-29
Identities = 63/112 (56%), Positives = 75/112 (66%), Gaps = 5/112 (4%)
Query: 5 VSLILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCS 64
V L L V++ H+ APAPS DCS LIL M DCLSFV++ TV KP+G CCS
Sbjct: 17 VLLSLSSVSVLGASHHHAT----APAPSVDCSTLILNMADCLSFVSSGGTVAKPEGTCCS 72
Query: 65 GLKSVLKTAPSCLCEAFKSSSQFGVVLNVTKALALPSACKVSASNSVTNCGC 116
GLK+VLK CLCEAFKSS+ GV LN+TKA LP+ACK+ A S+ CGC
Sbjct: 73 GLKTVLKADSQCLCEAFKSSASLGVTLNITKASTLPAACKLHAP-SIATCGC 123
>At2g13820 predicted GPI-anchored protein
Length = 169
Score = 115 bits (289), Expect = 1e-26
Identities = 58/111 (52%), Positives = 77/111 (69%), Gaps = 10/111 (9%)
Query: 5 VSLILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCS 64
+ +I VVA+ + + AH+A DCS+LIL M DCLSFVT+ STV KP+G CCS
Sbjct: 6 ILMIFSVVALMSGERAHAA---------VDCSSLILNMADCLSFVTSGSTVVKPEGTCCS 56
Query: 65 GLKSVLKTAPSCLCEAFKSSSQFGVVLNVTKALALPSACKVSASNSVTNCG 115
GLK+V++T P CLCEAFK+S G+ L+++KA +LPS CKV+A S CG
Sbjct: 57 GLKTVVRTGPECLCEAFKNSGSLGLTLDLSKAASLPSVCKVAAPPS-ARCG 106
>At1g36150 hypothetical protein
Length = 256
Score = 85.9 bits (211), Expect = 1e-17
Identities = 40/77 (51%), Positives = 51/77 (65%)
Query: 29 PAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCSGLKSVLKTAPSCLCEAFKSSSQFG 88
P+ S DCS++I M DCLSF+T ST P CC G+K+VL +P CLC A +SS + G
Sbjct: 39 PSNSTDCSSVIYSMVDCLSFLTVGSTDPSPTKTCCVGVKTVLNYSPKCLCSALESSREMG 98
Query: 89 VVLNVTKALALPSACKV 105
VL+ TKALA+P C V
Sbjct: 99 FVLDDTKALAMPKICNV 115
>At4g08670 lipid transfer protein - like predicted GPI-anchored
protein
Length = 194
Score = 83.2 bits (204), Expect = 7e-17
Identities = 38/82 (46%), Positives = 54/82 (65%), Gaps = 3/82 (3%)
Query: 28 APAP---SADCSNLILKMTDCLSFVTNDSTVTKPQGQCCSGLKSVLKTAPSCLCEAFKSS 84
APAP S+DCS +I M DCL ++ S TKP+ CC+G+++VL+ P C+C S+
Sbjct: 38 APAPGPSSSDCSTVIYSMMDCLGYLGVGSNETKPEKSCCTGIETVLQYNPQCICAGLVSA 97
Query: 85 SQFGVVLNVTKALALPSACKVS 106
+ G+ LN T+ALA P ACK+S
Sbjct: 98 GEMGIELNSTRALATPKACKLS 119
>At5g09370 putative lipid transfer protein, predicted GPI-anchored
protein
Length = 129
Score = 77.4 bits (189), Expect = 4e-15
Identities = 40/106 (37%), Positives = 60/106 (55%), Gaps = 11/106 (10%)
Query: 2 ASKVSLILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQ 61
++ SL+L V+++ + + H AS DC L++ + CL F++ T P
Sbjct: 5 STATSLLLLVLSVSS-PYVHGAS---------DCDTLVITLFPCLPFISIGGTADTPTAS 54
Query: 62 CCSGLKSVLKTAPSCLCEAFKSSSQFGVVLNVTKALALPSACKVSA 107
CCS LK++L T P CLCE K + G+ LNVTK+ LP ACK++A
Sbjct: 55 CCSSLKNILDTKPICLCEGLK-KAPLGIKLNVTKSATLPVACKLNA 99
>At3g22600 predicted GPI-anchored protein
Length = 170
Score = 68.9 bits (167), Expect = 1e-12
Identities = 31/82 (37%), Positives = 51/82 (61%), Gaps = 3/82 (3%)
Query: 25 SNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCSGLKSVLKTAPSCLCEAFK-S 83
S+ + + C+N ++ M+ CL+++T +ST P QCC+ L V++++P CLC+
Sbjct: 18 SSTMVSAQSSCTNALISMSPCLNYITGNST--SPNQQCCNQLSRVVQSSPDCLCQVLNGG 75
Query: 84 SSQFGVVLNVTKALALPSACKV 105
SQ G+ +N T+AL LP AC V
Sbjct: 76 GSQLGINVNQTQALGLPRACNV 97
>At2g48130 predicted GPI-anchored protein
Length = 183
Score = 66.6 bits (161), Expect = 7e-12
Identities = 33/85 (38%), Positives = 52/85 (60%), Gaps = 4/85 (4%)
Query: 31 PSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCSGLKSVLKTAPSCLCEAFKSS-SQFGV 89
PS+ C + + ++ CLS++T +ST P CCS L SV+K++P C+C A S G+
Sbjct: 26 PSSSCVSTLTTLSPCLSYITGNSTT--PSQPCCSRLDSVIKSSPQCICSAVNSPIPNIGL 83
Query: 90 VLNVTKALALPSACKVSASNSVTNC 114
+N T+AL LP+AC + + +T C
Sbjct: 84 NINRTQALQLPNACNIQ-TPPLTQC 107
>At4g14815 Expressed protein
Length = 156
Score = 66.2 bits (160), Expect = 9e-12
Identities = 36/106 (33%), Positives = 59/106 (54%), Gaps = 12/106 (11%)
Query: 1 MASKVSLILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQG 60
M ++ LIL + + + + SS C+N+++ M CLSF+T ++++ P
Sbjct: 1 MKPRMCLILFIALMRVMSIVSAQSS---------CTNVLISMAPCLSFITQNTSL--PSQ 49
Query: 61 QCCSGLKSVLKTAPSCLCEAFKSS-SQFGVVLNVTKALALPSACKV 105
QCC+ L V++ + CLC+ SQ G+ +N T+ALALP AC V
Sbjct: 50 QCCNQLAHVVRYSSECLCQVLDGGGSQLGINVNETQALALPKACHV 95
>At2g44300 predicted GPI-anchored protein
Length = 204
Score = 61.6 bits (148), Expect = 2e-10
Identities = 37/116 (31%), Positives = 57/116 (48%), Gaps = 14/116 (12%)
Query: 1 MASKVSLILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQG 60
MA+ ++LI+ + + A D + +C+ ++ M CL +V + P
Sbjct: 9 MATAIALIVVAMVVAAAD--------DKTKDKEECTEQLVGMATCLPYVQGQAK--SPTP 58
Query: 61 QCCSGLKSVLKTAPSCLCEAF--KSSSQFGVVLNVTKALALPSACKVSASNSVTNC 114
CCSGLK VL + CLC ++ G+ +NV+ ALALPS C +A VT C
Sbjct: 59 DCCSGLKQVLNSNKKCLCVIIQDRNDPDLGLQINVSLALALPSVCHAAA--DVTKC 112
>At3g43720 lipid-transfer protein-like, predicted GPI-anchored
protein
Length = 193
Score = 60.5 bits (145), Expect = 5e-10
Identities = 34/108 (31%), Positives = 55/108 (50%), Gaps = 9/108 (8%)
Query: 7 LILCVVAICAVDFAHSA-----SSNNAPAPSADCSNLILKMTDCLSFVT--NDSTVTKPQ 59
+++ VV I A H + S ++ P+ + DC ++ MT CLS+VT KP
Sbjct: 5 VVIAVVLIVASLTGHVSAQMDMSPSSGPSGAPDCMANLMNMTGCLSYVTVGEGGGAAKPD 64
Query: 60 GQCCSGLKSVLKTAPSCLCEAFKS--SSQFGVVLNVTKALALPSACKV 105
CC L +++++P CLC ++Q G+ ++ KAL LP C V
Sbjct: 65 KTCCPALAGLVESSPQCLCYLLSGDMAAQLGIKIDKAKALKLPGVCGV 112
>At2g44290 non-specific lipid transfer protein (nLTP) like protein
Length = 205
Score = 58.5 bits (140), Expect = 2e-09
Identities = 33/113 (29%), Positives = 55/113 (48%), Gaps = 6/113 (5%)
Query: 4 KVSLILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCC 63
K+ ++ +A+ V A+ + +C+ ++ M CL +V + P CC
Sbjct: 5 KIKVMATAIALIMVAMVVDAAGADKGKDKEECTAQLVGMATCLPYVQGKAK--SPTPDCC 62
Query: 64 SGLKSVLKTAPSCLCEAF--KSSSQFGVVLNVTKALALPSACKVSASNSVTNC 114
SGLK V+ + CLC ++ G+ +NV+ ALALPS C +A +T C
Sbjct: 63 SGLKQVINSDMKCLCMIIQERNDPDLGLQVNVSLALALPSVCHATA--DITKC 113
>At1g55260 unknown protein
Length = 184
Score = 58.5 bits (140), Expect = 2e-09
Identities = 27/83 (32%), Positives = 48/83 (57%), Gaps = 5/83 (6%)
Query: 34 DCSNLILKMTDCLSFVTNDSTVTKPQGQCCSGLKSVLKTAPSCLCEAF--KSSSQFGVVL 91
+C+N +++++ C+ +V D+ P CC+G V++ + C+C K Q G+ +
Sbjct: 32 ECTNQLIELSTCIPYVGGDAKA--PTKDCCAGFGQVIRKSEKCVCILVRDKDDPQLGIKI 89
Query: 92 NVTKALALPSACKVSASNSVTNC 114
N T A LPSAC ++A N +T+C
Sbjct: 90 NATLAAHLPSACHITAPN-ITDC 111
>At3g22611 predicted GPI-anchored protein
Length = 203
Score = 57.0 bits (136), Expect = 6e-09
Identities = 29/107 (27%), Positives = 56/107 (52%), Gaps = 8/107 (7%)
Query: 1 MASKVSLILCVVAICAVDFAHSASSNNAPAPSADCS-NLILKMTDCLSFVTNDSTV-TKP 58
M+ +SL++ ++A+ A+ P + C+ +++ ++ C+ F+TN S+ T P
Sbjct: 1 MSKIISLVVAMIAVLALPI------RGQQQPLSQCTPSMMTTVSPCMGFITNSSSNGTSP 54
Query: 59 QGQCCSGLKSVLKTAPSCLCEAFKSSSQFGVVLNVTKALALPSACKV 105
CC+ L+S+ CLC + F + +N T A++LP AC +
Sbjct: 55 SSDCCNSLRSLTTGGMGCLCLIVTGTVPFNIPINRTTAVSLPRACNM 101
>At2g27130 predicted GPI-anchored protein
Length = 159
Score = 56.2 bits (134), Expect = 1e-08
Identities = 33/115 (28%), Positives = 59/115 (50%), Gaps = 4/115 (3%)
Query: 7 LILCVVAICAVDFAHSASSNNAPAPSA--DCSNLILKMTDCLSFVTNDSTVTKPQGQCCS 64
L+L +++C+ + A PS+ +C +L ++DC S+V S KP+ CC
Sbjct: 10 LLLLFLSLCSGQSPPAPEPIAADGPSSPVNCLVSMLNVSDCFSYVQVGSNEIKPEAACCP 69
Query: 65 GLKSVLKTAPSCLCEAF--KSSSQFGVVLNVTKALALPSACKVSASNSVTNCGCE 117
L +++++P C+C + +S +FGV L+ +A L + C V A + G E
Sbjct: 70 ELAGMVQSSPECVCNLYGGGASPRFGVKLDKQRAEQLSTICGVKAPSPSLCSGSE 124
>At1g27950 GPI-anchored protein (LTPL)
Length = 193
Score = 50.4 bits (119), Expect = 5e-07
Identities = 32/113 (28%), Positives = 56/113 (49%), Gaps = 9/113 (7%)
Query: 7 LILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCSGL 66
L+L + I A A +A + A + +C+ K+T CL F T +T+ P +CC +
Sbjct: 8 LVLVTMTIVA-SIAAAAPAAPGGALADECNQDFQKVTLCLDFATGKATI--PSKKCCDAV 64
Query: 67 KSVLKTAPSCLCEAFKSSSQFGVVL-----NVTKALALPSACKVSASNSVTNC 114
+ + + P CLC + + G L K + LP++C++ + S+TNC
Sbjct: 65 EDIKERDPKCLCFVIQQAKTGGQALKDLGVQEDKLIQLPTSCQLH-NASITNC 116
>At1g05450 hypothetical protein
Length = 206
Score = 48.5 bits (114), Expect = 2e-06
Identities = 28/90 (31%), Positives = 44/90 (48%), Gaps = 8/90 (8%)
Query: 22 SASSNNAPAP-----SADCS-NLILKMTDCLSFVTNDSTVTKPQGQCCSGLKSVLKTAPS 75
S S+N+P + CS +++ +T C SF+T + P CC LKS+ T
Sbjct: 15 SLLSSNSPTSILAQINTPCSPSMLSSVTGCTSFLTGGGSF--PTSDCCGALKSLTGTGMD 72
Query: 76 CLCEAFKSSSQFGVVLNVTKALALPSACKV 105
CLC + + +N T A++LP AC +
Sbjct: 73 CLCLIVTAGVPISIPINRTLAISLPRACGI 102
>At2g48140 unknown protein
Length = 200
Score = 46.6 bits (109), Expect = 8e-06
Identities = 28/73 (38%), Positives = 39/73 (53%), Gaps = 4/73 (5%)
Query: 36 SNLILKMTDCLSFVTNDS--TVTKPQGQCCSGLKSVLKTAPSCLCEAFKSSSQFGV-VLN 92
S++I T CL+F+T S +VT P CC LK++ T C C ++ +N
Sbjct: 29 SSMISTFTPCLNFITGSSGGSVT-PTAGCCDSLKTLTNTGMGCACLILTANVPLPTGFIN 87
Query: 93 VTKALALPSACKV 105
T ALALP ACK+
Sbjct: 88 RTLALALPRACKM 100
>At3g58550 predicted GPI-anchored protein
Length = 177
Score = 44.7 bits (104), Expect = 3e-05
Identities = 27/80 (33%), Positives = 39/80 (48%), Gaps = 6/80 (7%)
Query: 35 CSNLILKMTDCLSFVTNDSTVTKPQGQCCSGLKSVLKTAPS--CLCEAFKSSSQ--FGVV 90
C + + + CL FVTN + P CCS LK + + CLC K G
Sbjct: 38 CQDAMSDLYSCLPFVTNKAKA--PDSTCCSTLKVKIDKGQTRKCLCTLVKDRDDPGLGFK 95
Query: 91 LNVTKALALPSACKVSASNS 110
++ +A++LPSAC V A+ S
Sbjct: 96 VDANRAMSLPSACHVPANIS 115
>At3g51590 lipid transfer protein-like protein
Length = 119
Score = 42.0 bits (97), Expect = 2e-04
Identities = 32/122 (26%), Positives = 54/122 (44%), Gaps = 18/122 (14%)
Query: 1 MASKVSLILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQG 60
MA +I C++ + + AS + + C + + CL+++TN +
Sbjct: 1 MAFTPKIITCLIVLTI----YMASPTES---TIQCGTVTSTLAQCLTYLTNSGPLPS--- 50
Query: 61 QCCSGLKSVLKTAPSC-----LCEAFKSSSQFGVVLNVTKALALPSACKVSASNSV---T 112
QCC G+KS+ + A + +CE K + + LN ALP+ C VS + T
Sbjct: 51 QCCVGVKSLYQLAQTTPDRKQVCECLKLAGKEIKGLNTDLVAALPTTCGVSIPYPISFST 110
Query: 113 NC 114
NC
Sbjct: 111 NC 112
>At2g38530 putative nonspecific lipid-transfer protein
Length = 118
Score = 41.6 bits (96), Expect = 2e-04
Identities = 35/119 (29%), Positives = 59/119 (49%), Gaps = 17/119 (14%)
Query: 5 VSLILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCS 64
V + C+V C + A ++N + C + + C++++T + +T QG CC+
Sbjct: 4 VMKLACMVLACMI-VAGPITANALMS----CGTVNGNLAGCIAYLTRGAPLT--QG-CCN 55
Query: 65 G---LKSVLKTAPSC--LCEAFKSSSQ-FGVVLNVTKALALPSACKVSASNSV---TNC 114
G LK++ T P C +S+++ G LN +A LPSACKV+ + TNC
Sbjct: 56 GVTNLKNMASTTPDRQQACRCLQSAAKAVGPGLNTARAAGLPSACKVNIPYKISASTNC 114
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.328 0.138 0.439
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,931,999
Number of Sequences: 26719
Number of extensions: 148372
Number of successful extensions: 510
Number of sequences better than 10.0: 54
Number of HSP's better than 10.0 without gapping: 30
Number of HSP's successfully gapped in prelim test: 24
Number of HSP's that attempted gapping in prelim test: 457
Number of HSP's gapped (non-prelim): 56
length of query: 185
length of database: 11,318,596
effective HSP length: 93
effective length of query: 92
effective length of database: 8,833,729
effective search space: 812703068
effective search space used: 812703068
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0314.16