BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0314.12
(180 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g02600 unknown protein 101 2e-22
At3g53530 unknown protein 97 6e-21
At2g37390 unknown protein 97 6e-21
At2g28660 unknown protein 85 2e-17
At3g24450 unknown protein 80 5e-16
At1g66240 copper homeostasis factor like protein 74 3e-14
At3g56240 copper homeostasis factor 71 4e-13
At5g27690 unknown protein (At5g27690) 68 2e-12
At1g56210 unknown protein 65 2e-11
At5g03380 farnesylated protein - like 65 3e-11
At3g06130 unknown protein 61 3e-10
At5g19090 unknown protein 61 4e-10
At4g08570 putative metal-binding isoprenylated protein 57 5e-09
At5g60800 unknown protein 55 2e-08
At1g23000 unknown protein 55 2e-08
At1g06330 hypothetical protein 54 5e-08
At5g17450 farnesylated protein ATFP6-like protein 53 8e-08
At5g63530 putative metal-binding protein 53 1e-07
At1g71050 isoprenylated like protein 53 1e-07
At1g22990 putative protein (At1g22990) 52 1e-07
>At5g02600 unknown protein
Length = 319
Score = 101 bits (251), Expect = 2e-22
Identities = 46/81 (56%), Positives = 65/81 (79%), Gaps = 2/81 (2%)
Query: 98 ENEPPKELQKTPTDNVFQVVVMRVAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVM 157
++ PP +K + + QVVV+RV++HC+GCAGKVK+HLSK++GVTS+NID +K+VTV
Sbjct: 235 QSSPPSPPEKNSSSD--QVVVLRVSLHCKGCAGKVKKHLSKLKGVTSYNIDFAAKKVTVT 292
Query: 158 GHISPVVVLESISKVKRAEFW 178
G ++P+ VL SISKVK A+FW
Sbjct: 293 GDVTPLTVLASISKVKNAQFW 313
>At3g53530 unknown protein
Length = 247
Score = 96.7 bits (239), Expect = 6e-21
Identities = 54/115 (46%), Positives = 72/115 (61%), Gaps = 11/115 (9%)
Query: 66 LVQSRSRSTSSDRESVSRVPKIKQRNLDQVEEENEPPKELQKTPTDNVFQVVVMRVAIHC 125
L S + T + ES + PK + P +E +K+ QVVV+RV++HC
Sbjct: 128 LAGSTGQDTVATEESEASAPK---------RGSSGPVEEKKKSSGSGSDQVVVLRVSLHC 178
Query: 126 --QGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESISKVKRAEFW 178
+GC GKVK+HLSKM+GVTSFNID SK+VTV G I+P+ VL +SKVK A+FW
Sbjct: 179 HCRGCQGKVKKHLSKMQGVTSFNIDFASKKVTVTGDITPLEVLGCLSKVKNAQFW 233
>At2g37390 unknown protein
Length = 259
Score = 96.7 bits (239), Expect = 6e-21
Identities = 44/81 (54%), Positives = 64/81 (78%)
Query: 98 ENEPPKELQKTPTDNVFQVVVMRVAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVM 157
E++ E + T D QVVV++V++HC+GC GKV++HL++M+GVTSFNID +K+VTV
Sbjct: 164 EDKTLTEKKTTCGDTDQQVVVLKVSLHCRGCEGKVRKHLARMQGVTSFNIDFAAKKVTVT 223
Query: 158 GHISPVVVLESISKVKRAEFW 178
G I+P+ +L+SISKVK A+FW
Sbjct: 224 GDITPLEILDSISKVKNAQFW 244
>At2g28660 unknown protein
Length = 237
Score = 84.7 bits (208), Expect = 2e-17
Identities = 37/58 (63%), Positives = 51/58 (87%)
Query: 121 VAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESISKVKRAEFW 178
V+IHC+GC GKV++H+SKMEGVTS+ ID+ +K+VTV+G I+PV ++ESISKVK A+ W
Sbjct: 161 VSIHCKGCEGKVRKHISKMEGVTSYTIDLATKKVTVVGKITPVGLVESISKVKFAQLW 218
>At3g24450 unknown protein
Length = 140
Score = 80.5 bits (197), Expect = 5e-16
Identities = 34/65 (52%), Positives = 53/65 (81%)
Query: 115 QVVVMRVAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESISKVKR 174
++V ++V++HC GCA KV++H+SK++GVT + +++ESK+V V G+I PV VLESI KVK
Sbjct: 74 KIVELKVSMHCYGCAKKVEKHISKLDGVTWYKVELESKKVVVKGNILPVDVLESICKVKN 133
Query: 175 AEFWN 179
A+ W+
Sbjct: 134 AQLWS 138
>At1g66240 copper homeostasis factor like protein
Length = 106
Score = 74.3 bits (181), Expect = 3e-14
Identities = 33/65 (50%), Positives = 49/65 (74%), Gaps = 1/65 (1%)
Query: 115 QVVVMRVAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESISKV-K 173
Q VV+RVA+ C+GC G VKR L KMEGV SF++D++ ++VTV G++ P VL++++K K
Sbjct: 33 QTVVLRVAMTCEGCVGAVKRVLGKMEGVESFDVDIKEQKVTVKGNVQPDAVLQTVTKTGK 92
Query: 174 RAEFW 178
+ FW
Sbjct: 93 KTAFW 97
>At3g56240 copper homeostasis factor
Length = 121
Score = 70.9 bits (172), Expect = 4e-13
Identities = 31/65 (47%), Positives = 46/65 (70%), Gaps = 1/65 (1%)
Query: 115 QVVVMRVAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESISKV-K 173
Q VV++V + CQGC G V R L KMEGV SF+ID++ ++VTV G++ P V +++SK K
Sbjct: 3 QTVVLKVGMSCQGCVGAVNRVLGKMEGVESFDIDIKEQKVTVKGNVEPEAVFQTVSKTGK 62
Query: 174 RAEFW 178
+ +W
Sbjct: 63 KTSYW 67
>At5g27690 unknown protein (At5g27690)
Length = 352
Score = 68.2 bits (165), Expect = 2e-12
Identities = 30/62 (48%), Positives = 48/62 (77%), Gaps = 1/62 (1%)
Query: 118 VMRVAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESISKVKR-AE 176
V+RV+IHC+GC K+K+ LSK++GV + NIDV+ ++VTV+G++ P ++++ I K R AE
Sbjct: 33 VLRVSIHCEGCKRKIKKILSKIDGVYTTNIDVKQQKVTVIGNVEPEILIKKIMKAGRHAE 92
Query: 177 FW 178
W
Sbjct: 93 LW 94
>At1g56210 unknown protein
Length = 364
Score = 65.5 bits (158), Expect = 2e-11
Identities = 35/103 (33%), Positives = 64/103 (61%), Gaps = 4/103 (3%)
Query: 75 SSDRESVSRVPKIKQRNLDQVEEENEPPKELQKTPTDNVFQVVVMRVAIHCQGCAGKVKR 134
+ + +S ++ + KQ+ Q++++ PP P ++ ++V+IHC+GC KVK+
Sbjct: 3 TDEMKSETKKTEHKQKQSTQIKQDLPPPTI---PPLPLPYKSCTLKVSIHCEGCKRKVKK 59
Query: 135 HLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESISKV-KRAE 176
L+ +EGV +IDV+ +VTV+G ISP ++L+ ++K K AE
Sbjct: 60 ILTSIEGVFKVDIDVKQHKVTVIGIISPEILLKKLNKAGKNAE 102
>At5g03380 farnesylated protein - like
Length = 392
Score = 64.7 bits (156), Expect = 3e-11
Identities = 30/84 (35%), Positives = 51/84 (60%), Gaps = 1/84 (1%)
Query: 92 LDQVEEENEPPKELQKTPTDNVFQVVVMRVAIHCQGCAGKVKRHLSKMEGVTSFNIDVES 151
+ + +EE + +K PTD VVM++ +HC+GC K+KR +GV ID +S
Sbjct: 1 MGEKKEETATKPQGEKKPTDGGITTVVMKLDMHCEGCGKKIKRIFKHFKGVEDVKIDYKS 60
Query: 152 KRVTVMGHISPVVVLESIS-KVKR 174
++TV+G++ PV V + ++ K+KR
Sbjct: 61 NKLTVIGNVDPVEVRDKVADKIKR 84
Score = 45.1 bits (105), Expect = 2e-05
Identities = 25/89 (28%), Positives = 48/89 (53%), Gaps = 1/89 (1%)
Query: 73 STSSDRESVSRVPKIKQRNLDQVEEENEPPKELQKTPTDNVFQ-VVVMRVAIHCQGCAGK 131
S +++ + + P ++ ++ EE+ + E + +P + VV++ +HC+GC K
Sbjct: 111 SPAAEEKPAEKKPAAVEKPGEKKEEKKKEEGEKKASPPPPPKESTVVLKTKLHCEGCEHK 170
Query: 132 VKRHLSKMEGVTSFNIDVESKRVTVMGHI 160
+KR ++K++GV S ID V V G I
Sbjct: 171 IKRIVNKIKGVNSVAIDSAKDLVIVKGII 199
>At3g06130 unknown protein
Length = 473
Score = 61.2 bits (147), Expect = 3e-10
Identities = 30/65 (46%), Positives = 42/65 (64%), Gaps = 1/65 (1%)
Query: 115 QVVVMRVAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESISKV-K 173
Q V++V IHC GC KVK+ L K+EGV + ID E +VTV G + P V+++ ++K K
Sbjct: 10 QTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDSEQGKVTVSGSVDPSVLIKKLAKSGK 69
Query: 174 RAEFW 178
AE W
Sbjct: 70 HAEIW 74
>At5g19090 unknown protein
Length = 587
Score = 60.8 bits (146), Expect = 4e-10
Identities = 30/65 (46%), Positives = 42/65 (64%), Gaps = 1/65 (1%)
Query: 115 QVVVMRVAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESISKV-K 173
Q V++V IHC GC KVK+ L K+EGV + ID E +VTV G++ P V+++ + K K
Sbjct: 10 QTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDAELGKVTVSGNVDPSVLIKKLLKSGK 69
Query: 174 RAEFW 178
AE W
Sbjct: 70 HAEIW 74
>At4g08570 putative metal-binding isoprenylated protein
Length = 150
Score = 57.0 bits (136), Expect = 5e-09
Identities = 31/66 (46%), Positives = 44/66 (65%), Gaps = 2/66 (3%)
Query: 115 QVVVMRVA-IHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESI-SKV 172
Q V +RVA I C+GC K+K LS ++GV S ++DV+ ++VTV G+I P VLE+ S
Sbjct: 26 QTVALRVARIDCEGCERKIKHVLSGVKGVKSVDVDVKLQKVTVTGYIDPKKVLEAAKSTK 85
Query: 173 KRAEFW 178
K+ E W
Sbjct: 86 KKVELW 91
>At5g60800 unknown protein
Length = 283
Score = 55.1 bits (131), Expect = 2e-08
Identities = 27/80 (33%), Positives = 45/80 (55%), Gaps = 1/80 (1%)
Query: 97 EENEPPKELQKTPTDNVFQVVVMRVAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTV 156
EE + P K P + V+++ HCQGC GK+++ ++K +GV +D E +TV
Sbjct: 115 EEKKKPDNNDKKPKETPVTTAVLKLNFHCQGCIGKIQKTVTKTKGVNGLTMDKEKNLLTV 174
Query: 157 MGHISPVVVLESIS-KVKRA 175
G + ++E +S K+KRA
Sbjct: 175 KGTMDVKKLVEILSEKLKRA 194
Score = 41.6 bits (96), Expect = 2e-04
Identities = 20/84 (23%), Positives = 47/84 (55%), Gaps = 4/84 (4%)
Query: 88 KQRNLDQVEEENEPPKELQKTPTDNVFQVVVMRVAIHCQGCAGKVKRHLSKMEGVTSFNI 147
+++N +++ K+ +TP+ V V++V +HC+GCA ++ + + +GV +
Sbjct: 3 EKKNEGDNKKKGGDNKKKNETPSITV----VLKVDMHCEGCASRIVKCVRSFQGVETVKS 58
Query: 148 DVESKRVTVMGHISPVVVLESISK 171
+ + ++TV G + PV + E + +
Sbjct: 59 ESATGKLTVTGALDPVKLREKLEE 82
>At1g23000 unknown protein
Length = 358
Score = 55.1 bits (131), Expect = 2e-08
Identities = 26/66 (39%), Positives = 39/66 (58%), Gaps = 1/66 (1%)
Query: 115 QVVVMRVAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESISKV-K 173
Q +RV IHC+GC KVK+ L ++EGV I+ E ++VTV G + ++ + K K
Sbjct: 13 QTFSLRVNIHCEGCNKKVKKLLQRIEGVCHVKIEAEHQKVTVSGSVDSATLINKLVKAGK 72
Query: 174 RAEFWN 179
AE W+
Sbjct: 73 HAELWS 78
>At1g06330 hypothetical protein
Length = 159
Score = 53.9 bits (128), Expect = 5e-08
Identities = 27/65 (41%), Positives = 38/65 (57%), Gaps = 1/65 (1%)
Query: 115 QVVVMRVAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESISKV-K 173
Q + MRV + C GC +VK L KM GV + ID+ ++VTV G+ VL+ + K +
Sbjct: 11 QTIEMRVHMDCVGCESRVKNALQKMRGVDAVEIDMVQQKVTVTGYADQKKVLKKVRKTGR 70
Query: 174 RAEFW 178
RAE W
Sbjct: 71 RAELW 75
>At5g17450 farnesylated protein ATFP6-like protein
Length = 149
Score = 53.1 bits (126), Expect = 8e-08
Identities = 25/65 (38%), Positives = 41/65 (62%), Gaps = 1/65 (1%)
Query: 115 QVVVMRVAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESI-SKVK 173
Q V ++V + C GC +V+ + +M+GV S ++ + R+TV GH+ P VL+ + S K
Sbjct: 26 QTVDIKVKMDCDGCERRVRNVVRRMKGVKSVEVNRKQSRITVNGHVDPNKVLKRVKSTGK 85
Query: 174 RAEFW 178
+AEFW
Sbjct: 86 KAEFW 90
>At5g63530 putative metal-binding protein
Length = 355
Score = 52.8 bits (125), Expect = 1e-07
Identities = 31/107 (28%), Positives = 56/107 (51%), Gaps = 2/107 (1%)
Query: 65 KLVQSRSRSTSSDRESVSRVPKIKQRNLDQVEEENEPPKELQKTPTDNVFQVVVMRVAIH 124
K++ R T + +S +P + EE+ +E + P V VV++V +H
Sbjct: 123 KVLARVQRKTHRQVQLLSPIPPPPPPPEKKAEEDKPIVEEKKVEPP--VVVTVVLKVHMH 180
Query: 125 CQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESISK 171
C+ CA ++K+ + +M+GV S D++S +VTV G P ++E + K
Sbjct: 181 CEACATEIKKRIMRMKGVESAESDLKSSQVTVKGVFEPQKLVEYVYK 227
Score = 48.1 bits (113), Expect = 2e-06
Identities = 33/108 (30%), Positives = 51/108 (46%), Gaps = 8/108 (7%)
Query: 65 KLVQSRSRSTSSDRESVSRVPKIKQRNLDQVEEENEPPKELQKTPTDNVFQVVVMRVAIH 124
K V + + SD++ P+ + N D E+ E P Q VV++V +H
Sbjct: 30 KKVDAEKKGEDSDKK-----PQEGESNKDSKEDSAPAAPEAPAPPPPP--QEVVLKVYMH 82
Query: 125 CQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGH-ISPVVVLESISK 171
C+GCA KV+R L EGV D ++ +V V G P+ VL + +
Sbjct: 83 CEGCARKVRRCLKGFEGVEDVMTDCKTGKVVVKGEKADPLKVLARVQR 130
>At1g71050 isoprenylated like protein
Length = 152
Score = 52.8 bits (125), Expect = 1e-07
Identities = 26/67 (38%), Positives = 41/67 (60%), Gaps = 1/67 (1%)
Query: 113 VFQVVVMRVAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESISKV 172
V Q V ++V + C GC +VK +S M+GV S ++ + +VTV G++ P VL+ I +
Sbjct: 26 VMQTVNIKVKMDCDGCERRVKNAVSSMKGVKSVEVNRKIHKVTVSGYVEPKKVLKRIERT 85
Query: 173 -KRAEFW 178
K+AE W
Sbjct: 86 GKKAEIW 92
>At1g22990 putative protein (At1g22990)
Length = 152
Score = 52.4 bits (124), Expect = 1e-07
Identities = 25/76 (32%), Positives = 43/76 (55%), Gaps = 2/76 (2%)
Query: 105 LQKTPTDNVFQVVVMRVAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVV 164
++K V Q V ++V I C GC K+K +S ++G S ++ + +VTV G++ P
Sbjct: 19 IRKRKKRKVMQTVNIKVKIDCDGCERKIKNAVSSIKGAKSVEVNRKMHKVTVSGYVDPKK 78
Query: 165 VLESISKV--KRAEFW 178
VL+++ K+AE W
Sbjct: 79 VLKTVQSTGKKKAELW 94
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.317 0.129 0.365
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,709,343
Number of Sequences: 26719
Number of extensions: 139031
Number of successful extensions: 627
Number of sequences better than 10.0: 74
Number of HSP's better than 10.0 without gapping: 55
Number of HSP's successfully gapped in prelim test: 19
Number of HSP's that attempted gapping in prelim test: 556
Number of HSP's gapped (non-prelim): 87
length of query: 180
length of database: 11,318,596
effective HSP length: 93
effective length of query: 87
effective length of database: 8,833,729
effective search space: 768534423
effective search space used: 768534423
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0314.12