BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0290.12
(143 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g34190 unknown protein 152 5e-38
At1g44575 Photosystem II chlorophyll-binding protein PsbS 38 0.002
At2g21970 unknown protein 35 0.014
At2g30390 putative ferrochelatase precusor 35 0.018
At3g22840 early light-induced protein 33 0.052
At1g34000 unknown protein 32 0.12
At1g25540 hypothetical protein 31 0.20
At4g14690 light induced protein like 31 0.26
At5g02120 one helix protein (OHP) 30 0.57
At4g28025 unknown protein 29 0.75
At3g12340 hypothetical protein 29 0.75
At3g08940 Lhcb4-protein (CP29, Lhcb4.2) 29 0.98
At1g71500 unknown protein 28 1.3
At5g47110 Lil3 protein 28 2.2
At4g17600 Lil3 protein (Lil3:1) 28 2.2
At3g08040 putative integral membrane protein 28 2.2
At1g56670 lipase homolog (Lip-4) 27 3.7
At5g01530 chlorophyll a/b-binding protein CP29 27 4.9
At3g55560 unknown protein 27 4.9
At3g47470 light-harvesting chlorophyll a/b-binding protein (Cab4) 27 4.9
>At4g34190 unknown protein
Length = 146
Score = 152 bits (385), Expect = 5e-38
Identities = 89/147 (60%), Positives = 110/147 (74%), Gaps = 5/147 (3%)
Query: 1 MALATQTSASLSISFRDACVFPAAKYS---ATARFPLPNLVPSRASFATGTPLL-IRRSH 56
MAL+ Q SASL+ S ++ A + +T R +P L R++FA+G+PLL ++ S
Sbjct: 1 MALS-QVSASLAFSLPNSGALKLATITNPTSTCRVHVPQLAGIRSTFASGSPLLPLKLSM 59
Query: 57 GRKLACRAMPVSIRSEQSTKDGNGLDVWLGRLAMIGFAAGITVEVATGKGLLENFGVASP 116
R+ RA VSIRSEQST+ +GLD+WLGR AM+GFA ITVE++TGKGLLENFGVASP
Sbjct: 60 TRRGGNRAASVSIRSEQSTEGSSGLDIWLGRGAMVGFAVAITVEISTGKGLLENFGVASP 119
Query: 117 LPTVALAVTALVGVLTAVFIFQSASKN 143
LPTVALAVTALVGVL AVFIFQS+SKN
Sbjct: 120 LPTVALAVTALVGVLAAVFIFQSSSKN 146
>At1g44575 Photosystem II chlorophyll-binding protein PsbS
Length = 265
Score = 38.1 bits (87), Expect = 0.002
Identities = 16/37 (43%), Positives = 26/37 (70%)
Query: 82 DVWLGRLAMIGFAAGITVEVATGKGLLENFGVASPLP 118
++++GR+AMIGFAA + E TGKG+L + + +P
Sbjct: 90 ELFVGRVAMIGFAASLLGEALTGKGILAQLNLETGIP 126
Score = 34.3 bits (77), Expect = 0.023
Identities = 18/56 (32%), Positives = 30/56 (53%), Gaps = 2/56 (3%)
Query: 82 DVWLGRLAMIGFAAGITVEVATGKGLLENFGVASPLPTVALAVTALVGVLTAVFIF 137
++++GRLA +G A + E+ TGKG L + + +P + L+ V A F F
Sbjct: 194 ELFVGRLAQLGIAFSLIGEIITGKGALAQLNIETGIPIQDIEPLVLLNV--AFFFF 247
>At2g21970 unknown protein
Length = 202
Score = 35.0 bits (79), Expect = 0.014
Identities = 21/71 (29%), Positives = 34/71 (47%), Gaps = 2/71 (2%)
Query: 73 QSTKDGNGLDVWLGRLAMIGFAAGITVEVATGKGLLENFGVASPLPTVALAVTALVGVLT 132
+S+K + GRLAMI FA +T E+ TG L + V + + A+
Sbjct: 96 ESSKKSQDFETISGRLAMIVFAVTVTEEIVTGNSLFKKLDVEGLSEAIGAGLAAM--GCA 153
Query: 133 AVFIFQSASKN 143
A+F + + S+N
Sbjct: 154 AMFAWLTISRN 164
>At2g30390 putative ferrochelatase precusor
Length = 512
Score = 34.7 bits (78), Expect = 0.018
Identities = 15/36 (41%), Positives = 21/36 (57%)
Query: 82 DVWLGRLAMIGFAAGITVEVATGKGLLENFGVASPL 117
+ W GR AM+ A + +EV TGKG L +G+ L
Sbjct: 477 ETWNGRAAMLAVLALLVLEVTTGKGFLHQWGILPSL 512
>At3g22840 early light-induced protein
Length = 195
Score = 33.1 bits (74), Expect = 0.052
Identities = 22/60 (36%), Positives = 32/60 (52%), Gaps = 3/60 (5%)
Query: 86 GRLAMIGFAAGITVEVATGKGLLENF---GVASPLPTVALAVTALVGVLTAVFIFQSASK 142
GRLAM+GF A + VE++ G+ +L GV+ L T A+ A + L +S SK
Sbjct: 102 GRLAMVGFVAALAVELSKGENVLAQISDGGVSWFLGTTAILTLASLVPLFKGISVESKSK 161
>At1g34000 unknown protein
Length = 172
Score = 32.0 bits (71), Expect = 0.12
Identities = 18/35 (51%), Positives = 20/35 (56%), Gaps = 7/35 (20%)
Query: 86 GRLAMIGFAAGITVEVATG-------KGLLENFGV 113
GR AM GFA G+ E ATG K LL NFG+
Sbjct: 134 GRWAMFGFAVGMLTEYATGSDLVDQVKILLSNFGI 168
>At1g25540 hypothetical protein
Length = 809
Score = 31.2 bits (69), Expect = 0.20
Identities = 24/67 (35%), Positives = 34/67 (49%), Gaps = 8/67 (11%)
Query: 50 LLIRRSHGRKLACRAMPVSIRSEQSTKDGNGLDVWLGRLAMIGFAAGITVEVATGKGLLE 109
L+I SHG AC ++ T+D +D++L L+ I F G EVAT +GL E
Sbjct: 29 LVIFNSHGSYCACL-----VQRSGWTRD---VDIFLHWLSSIQFGGGGFNEVATAEGLAE 80
Query: 110 NFGVASP 116
+ SP
Sbjct: 81 ALMMFSP 87
>At4g14690 light induced protein like
Length = 193
Score = 30.8 bits (68), Expect = 0.26
Identities = 18/44 (40%), Positives = 26/44 (58%), Gaps = 3/44 (6%)
Query: 86 GRLAMIGFAAGITVEVATGKGL---LENFGVASPLPTVALAVTA 126
GRLAM+GF A I +E++ G+ + + + GV L T AL A
Sbjct: 100 GRLAMVGFVAAIAMELSKGENVFAQISDGGVGWFLGTTALLTLA 143
Score = 25.8 bits (55), Expect = 8.3
Identities = 15/42 (35%), Positives = 22/42 (51%), Gaps = 2/42 (4%)
Query: 69 IRSEQSTKD--GNGLDVWLGRLAMIGFAAGITVEVATGKGLL 108
IR+E +K + ++W GR AM+G A E TG L+
Sbjct: 152 IRAEAKSKGFMTSDAELWNGRFAMLGLVALAFTEYVTGGTLV 193
>At5g02120 one helix protein (OHP)
Length = 110
Score = 29.6 bits (65), Expect = 0.57
Identities = 13/32 (40%), Positives = 17/32 (52%)
Query: 82 DVWLGRLAMIGFAAGITVEVATGKGLLENFGV 113
++W R MIG VE+ KG+LE GV
Sbjct: 69 EIWNSRACMIGLIGTFIVELILNKGILELIGV 100
>At4g28025 unknown protein
Length = 157
Score = 29.3 bits (64), Expect = 0.75
Identities = 15/59 (25%), Positives = 30/59 (50%), Gaps = 1/59 (1%)
Query: 50 LLIRRSHGRKLACRAMPVSIRSEQSTKDGNGLDVWLGRLAMIGFAAGITVEVATGKGLL 108
+++ ++LA + M I+ + + ++ G A+IG G+ +E TGKG+L
Sbjct: 82 IMVDPLEAKRLASKQME-EIKGREKQQRRREIEAINGAWAIIGLMIGLVIEAQTGKGIL 139
>At3g12340 hypothetical protein
Length = 647
Score = 29.3 bits (64), Expect = 0.75
Identities = 17/51 (33%), Positives = 27/51 (52%), Gaps = 5/51 (9%)
Query: 86 GRLAMIGFAAGITVEVATGKGLLENFGVASPLPTVALAVTALVGVLTAVFI 136
GR+A +G A + VE+ATGK +L P+V V ++A+F+
Sbjct: 128 GRIAGLGLTALLLVELATGKSVLNYH-----TPSVVFLQIYFVAAVSAMFV 173
>At3g08940 Lhcb4-protein (CP29, Lhcb4.2)
Length = 287
Score = 28.9 bits (63), Expect = 0.98
Identities = 16/38 (42%), Positives = 21/38 (55%), Gaps = 2/38 (5%)
Query: 87 RLAMIGFAAGITVEVATGKGLLENFG--VASPLPTVAL 122
RLAM+GF ATGKG L N+ ++ PL T +
Sbjct: 244 RLAMVGFLGFAVQAAATGKGPLNNWATHLSDPLHTTII 281
>At1g71500 unknown protein
Length = 287
Score = 28.5 bits (62), Expect = 1.3
Identities = 13/27 (48%), Positives = 18/27 (66%)
Query: 86 GRLAMIGFAAGITVEVATGKGLLENFG 112
G+ A+IGF + E+ TGKGLL+ G
Sbjct: 248 GKAAVIGFLLLLDFELLTGKGLLKGTG 274
>At5g47110 Lil3 protein
Length = 258
Score = 27.7 bits (60), Expect = 2.2
Identities = 13/27 (48%), Positives = 16/27 (59%)
Query: 86 GRLAMIGFAAGITVEVATGKGLLENFG 112
GR AMIGF V+ TG GL++ G
Sbjct: 175 GRAAMIGFFMAYFVDSLTGVGLVDQMG 201
>At4g17600 Lil3 protein (Lil3:1)
Length = 262
Score = 27.7 bits (60), Expect = 2.2
Identities = 13/27 (48%), Positives = 16/27 (59%)
Query: 86 GRLAMIGFAAGITVEVATGKGLLENFG 112
GR AMIGF V+ TG GL++ G
Sbjct: 178 GRAAMIGFFMAYFVDSLTGVGLVDQMG 204
>At3g08040 putative integral membrane protein
Length = 526
Score = 27.7 bits (60), Expect = 2.2
Identities = 16/45 (35%), Positives = 21/45 (46%)
Query: 76 KDGNGLDVWLGRLAMIGFAAGITVEVATGKGLLENFGVASPLPTV 120
KD N + R+ +GF G+ + V G GL GV S P V
Sbjct: 380 KDYNKVTAVASRVLQMGFVLGLGLSVFVGLGLYFGAGVFSKDPAV 424
>At1g56670 lipase homolog (Lip-4)
Length = 373
Score = 26.9 bits (58), Expect = 3.7
Identities = 19/47 (40%), Positives = 20/47 (42%), Gaps = 2/47 (4%)
Query: 50 LLIRRSHGRKLACRAMPVSIRSEQSTKDGNGLDVWLGRLAMIGFAAG 96
LLI R R +C A PV S D GL LG IGF G
Sbjct: 23 LLILRQPSRAASCTARPVIFNFGDSNSDTGGLVAGLG--YPIGFPNG 67
>At5g01530 chlorophyll a/b-binding protein CP29
Length = 290
Score = 26.6 bits (57), Expect = 4.9
Identities = 15/38 (39%), Positives = 20/38 (52%), Gaps = 2/38 (5%)
Query: 87 RLAMIGFAAGITVEVATGKGLLENFG--VASPLPTVAL 122
RLAM+ F ATGKG L N+ ++ PL T +
Sbjct: 247 RLAMVAFLGFAVQAAATGKGPLNNWATHLSDPLHTTII 284
>At3g55560 unknown protein
Length = 310
Score = 26.6 bits (57), Expect = 4.9
Identities = 14/36 (38%), Positives = 21/36 (57%), Gaps = 4/36 (11%)
Query: 94 AAGITVEVATGKGLLENFGVASPL----PTVALAVT 125
AAG+T+ +A +G + GVA PL P + +A T
Sbjct: 192 AAGLTIYLAGAQGQVVGGGVAGPLIASGPVIVIAAT 227
>At3g47470 light-harvesting chlorophyll a/b-binding protein (Cab4)
Length = 251
Score = 26.6 bits (57), Expect = 4.9
Identities = 13/36 (36%), Positives = 19/36 (52%)
Query: 75 TKDGNGLDVWLGRLAMIGFAAGITVEVATGKGLLEN 110
T++ ++ GRLAM+ F + TGKG EN
Sbjct: 198 TQEAKEKELANGRLAMLAFLGFVVQHNVTGKGPFEN 233
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.321 0.133 0.378
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,649,575
Number of Sequences: 26719
Number of extensions: 98056
Number of successful extensions: 226
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 19
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 201
Number of HSP's gapped (non-prelim): 30
length of query: 143
length of database: 11,318,596
effective HSP length: 89
effective length of query: 54
effective length of database: 8,940,605
effective search space: 482792670
effective search space used: 482792670
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0290.12