BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0281b.2
(124 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g14430 putative non-LTR retroelement reverse transcriptase 56 4e-09
At3g32110 non-LTR reverse transcriptase, putative 54 3e-08
At2g19100 putative non-LTR retroelement reverse transcriptase 49 8e-07
At2g17910 putative non-LTR retroelement reverse transcriptase 49 8e-07
At2g05550 putative non-LTR retroelement reverse transcriptase 48 1e-06
At1g43760 reverse transcriptase, putative 48 1e-06
At1g24640 hypothetical protein 48 1e-06
At1g10160 putative reverse transcriptase 48 1e-06
At2g31080 putative non-LTR retroelement reverse transcriptase 46 4e-06
At2g45230 putative non-LTR retroelement reverse transcriptase 46 5e-06
At2g18820 putative non-LTR retroelement reverse transcriptase 45 9e-06
At2g15510 putative non-LTR retroelement reverse transcriptase 45 9e-06
At2g05980 putative non-LTR retroelement reverse transcriptase 45 9e-06
At2g01550 putative non-LTR retroelement reverse transcriptase 45 9e-06
At1g31100 hypothetical protein 43 3e-05
At1g25430 hypothetical protein 43 5e-05
At2g16680 putative non-LTR retroelement reverse transcriptase 41 1e-04
At1g37050 putative non-LTR retroelement reverse transcriptase 41 1e-04
At3g31420 hypothetical protein 41 2e-04
At2g01840 putative non-LTR retroelement reverse transcriptase 40 3e-04
>At2g14430 putative non-LTR retroelement reverse transcriptase
Length = 1277
Score = 56.2 bits (134), Expect = 4e-09
Identities = 34/115 (29%), Positives = 52/115 (44%), Gaps = 5/115 (4%)
Query: 9 IAGDFNVVARSEERMGISDSIYGRSDMADFKAFLSNLSLIEPPAVGKKYTWF--RPNGQA 66
+ GDFN + E S + M DF+ + SL + G ++TW R NG
Sbjct: 385 LCGDFNEILDGSEHSNYDTSPFTPLGMRDFQEVVRYSSLTDLGYHGPRFTWCNKRENGLI 444
Query: 67 ASRLDRFLLSSDWCSCWPHGVQTVMARSLSDHC--PVMFRSTDMNWGPRPFRVLN 119
+LD+ L++ WC +PH A SDH +M + + G RPF+ +N
Sbjct: 445 CKKLDKVLVNDSWCITFPHSYSVFEAGGCSDHTRGRIMLEAAAIG-GRRPFKFVN 498
>At3g32110 non-LTR reverse transcriptase, putative
Length = 1911
Score = 53.5 bits (127), Expect = 3e-08
Identities = 40/121 (33%), Positives = 55/121 (45%), Gaps = 8/121 (6%)
Query: 9 IAGDFNVVARSEERMGISDSIYGRSDMADFKAFLSNLSLIEPPAVGKKYTWFRPNGQ--- 65
I GDFN + R +ER G + + SD F ++++ SLI+ G K+TW R +
Sbjct: 699 IGGDFNTIVRLDERSGGNGRL--SSDSLAFGEWINDHSLIDLGFKGNKFTWKRGREERFF 756
Query: 66 AASRLDRFLLSSDWCSCWPHGVQTVMARSLSDHCPVMFRST---DMNWGPRPFRVLNCWF 122
A RLDR L + W + SDH P+ + T N G RPFR W
Sbjct: 757 VAKRLDRVLCCAHARLKWQEASVLHLPFLASDHAPLYVQLTPEVSGNRGRRPFRFEAAWL 816
Query: 123 S 123
S
Sbjct: 817 S 817
>At2g19100 putative non-LTR retroelement reverse transcriptase
Length = 1447
Score = 48.5 bits (114), Expect = 8e-07
Identities = 33/117 (28%), Positives = 49/117 (41%), Gaps = 6/117 (5%)
Query: 9 IAGDFNVVARSEERMGISDSIYGRSDMADFKAFLSNLSLIEPPAVGKKYTWF--RPNGQA 66
I GDFN EE + D+ M DF++ ++ SL + G YTW R +
Sbjct: 547 IFGDFNETLELEEHSKVEDNPVVSMGMRDFRSMVNYCSLTDMAHHGPLYTWSNKREHDLI 606
Query: 67 ASRLDRFLLSSDWCSCWPHGVQTVMARSLSDHCPVMFRSTD----MNWGPRPFRVLN 119
A +LDR +++ W +P A DH D + G RPF+ +N
Sbjct: 607 AKKLDRVMVNDVWTQSFPQSYSVFEAGGCLDHLRGRINLNDGPGSIVRGKRPFKFVN 663
>At2g17910 putative non-LTR retroelement reverse transcriptase
Length = 1344
Score = 48.5 bits (114), Expect = 8e-07
Identities = 31/105 (29%), Positives = 52/105 (49%), Gaps = 4/105 (3%)
Query: 7 FCIAGDFNVVARSEERMGISDSIYGRSDMADFKAFLSNLSLIEPPAVGKKYTWF--RPNG 64
+C+ GDFN + ++E++G S F+ L N S+ E + G +TW R +
Sbjct: 109 WCLIGDFNDIRSNDEKLG--GPRRSPSSFQCFEHMLLNCSMHELGSTGNSFTWGGNRNDQ 166
Query: 65 QAASRLDRFLLSSDWCSCWPHGVQTVMARSLSDHCPVMFRSTDMN 109
+LDR + W S +P+ Q + + SDH PV+ + T+ N
Sbjct: 167 WVQCKLDRCFGNPAWFSIFPNAHQWFLEKFGSDHRPVLVKFTNDN 211
>At2g05550 putative non-LTR retroelement reverse transcriptase
Length = 977
Score = 48.1 bits (113), Expect = 1e-06
Identities = 32/117 (27%), Positives = 48/117 (40%), Gaps = 6/117 (5%)
Query: 9 IAGDFNVVARSEERMGISDSIYGRSDMADFKAFLSNLSLIEPPAVGKKYTWFRPNGQ--A 66
I GDFNV +E + S M DF+ + + L + + G YTW Q
Sbjct: 346 IFGDFNVTLDMKEHSNCDSNPVVTSGMRDFQQMIRSCDLSDMASHGPLYTWCNKKEQDLI 405
Query: 67 ASRLDRFLLSSDWCSCWPHGVQTVMARSLSDH--CPVMFRSTDMN--WGPRPFRVLN 119
+LDR +++ W + A SDH C +M GP+PF+ +N
Sbjct: 406 LKKLDRVMVNDIWAQSFSQAYNVFEAGGCSDHLRCRIMLNDAAERSVKGPKPFKFVN 462
>At1g43760 reverse transcriptase, putative
Length = 622
Score = 48.1 bits (113), Expect = 1e-06
Identities = 23/94 (24%), Positives = 45/94 (47%), Gaps = 2/94 (2%)
Query: 9 IAGDFNVVARSEERMGISDSIYGRSDMADFKAFLSNLSLIEPPAVGKKYTW--FRPNGQA 66
+ GDF+ +A + + + + + +F+ L + L++ P+ G YTW + +
Sbjct: 219 LVGDFDQIAATSDHYSVLQTSIPMRGLEEFQNCLRDSDLVDIPSRGVHYTWSNHQDDNPI 278
Query: 67 ASRLDRFLLSSDWCSCWPHGVQTVMARSLSDHCP 100
+LDR + + DW S +P + +SDH P
Sbjct: 279 IRKLDRAIANGDWFSSFPSAIAVFELSGVSDHSP 312
>At1g24640 hypothetical protein
Length = 1270
Score = 47.8 bits (112), Expect = 1e-06
Identities = 35/103 (33%), Positives = 50/103 (47%), Gaps = 10/103 (9%)
Query: 7 FCIAGDFNVVARSEERMGISDSIYGRSDMADFKAF---LSNLSLIEPPAVGKKYTWFRPN 63
+C+ GDFN + + E+ G RSD+ D KAF + L+E PA G +TW
Sbjct: 94 WCMFGDFNDILHNGEKNGGPR----RSDL-DCKAFNEMIKGCDLVEMPAHGNGFTWAGRR 148
Query: 64 GQ--AASRLDRFLLSSDWCSCWPHGVQTVMARSLSDHCPVMFR 104
G RLDR + +W +P QT + SDH PV+ +
Sbjct: 149 GDHWIQCRLDRAFGNKEWFCFFPVSNQTFLDFRGSDHRPVLIK 191
>At1g10160 putative reverse transcriptase
Length = 1118
Score = 47.8 bits (112), Expect = 1e-06
Identities = 26/99 (26%), Positives = 47/99 (47%), Gaps = 2/99 (2%)
Query: 4 ISCFCIAGDFNVVARSEERMGISDSIYGRSDMADFKAFLSNLSLIEPPAVGKKYTW--FR 61
++ + + GDFN +A + E I+ S+ M D + L + L + P+ G +TW +
Sbjct: 134 VTPWLLLGDFNQIAAASEHYSINQSLLNLRGMEDLQCCLRDSQLSDLPSRGVFFTWSNHQ 193
Query: 62 PNGQAASRLDRFLLSSDWCSCWPHGVQTVMARSLSDHCP 100
+ +LDR L + +W + +P + SDH P
Sbjct: 194 QDNPILRKLDRALANGEWFAVFPSALAVFDPPGDSDHAP 232
>At2g31080 putative non-LTR retroelement reverse transcriptase
Length = 1231
Score = 46.2 bits (108), Expect = 4e-06
Identities = 31/96 (32%), Positives = 47/96 (48%), Gaps = 5/96 (5%)
Query: 9 IAGDFNVVARSEERMGISDSIYGRSDMADFKAFLSNLSLIEPPAVGKKYTWFRPNGQA-- 66
I GDFN + +ERMG + + D F +++ LSLI+ G K+TW R ++
Sbjct: 35 IGGDFNTILWVDERMGGNGRL--SPDSLAFGDWINELSLIDLGFKGNKFTWRRGRQESTV 92
Query: 67 -ASRLDRFLLSSDWCSCWPHGVQTVMARSLSDHCPV 101
A RLDR + + W V + + SDH P+
Sbjct: 93 VAKRLDRVFVCAHARLKWQEAVVSHLPFMASDHAPL 128
>At2g45230 putative non-LTR retroelement reverse transcriptase
Length = 1374
Score = 45.8 bits (107), Expect = 5e-06
Identities = 27/104 (25%), Positives = 47/104 (44%), Gaps = 4/104 (3%)
Query: 9 IAGDFNVVARSEERMGISDSIYGRSDMADFKAFLSNLSLIEPPAVGKKYTWF--RPNGQA 66
+ GDFN + E++G S +F+ L++ L E G +++W+ R +
Sbjct: 133 LTGDFNELVDPSEKIG--GPARKESSCLEFRQMLNSCGLWEVNHSGYQFSWYGNRNDELV 190
Query: 67 ASRLDRFLLSSDWCSCWPHGVQTVMARSLSDHCPVMFRSTDMNW 110
RLDR + + W +P T + + SDH P++ NW
Sbjct: 191 QCRLDRTVANQAWMELFPQAKATYLQKICSDHSPLINNLVGDNW 234
>At2g18820 putative non-LTR retroelement reverse transcriptase
Length = 1412
Score = 45.1 bits (105), Expect = 9e-06
Identities = 33/112 (29%), Positives = 48/112 (42%), Gaps = 3/112 (2%)
Query: 11 GDFNVVARSEERMGISDSIYGRSDMADFKAFLSNLSLIEPPAVGKKYTWF--RPNGQAAS 68
GDFN + EE + S M DF+ + SL + G +TW R G
Sbjct: 443 GDFNETLKMEEHSSYAVSPMVTPGMRDFQIVVRYCSLEDMRTHGPLFTWGNKRNEGLICK 502
Query: 69 RLDRFLLSSDWCSCWPHGVQTVMARSLSDHCPVMFRSTDMNWGPR-PFRVLN 119
+LDR LL+ ++ S +PH + + SDH F P+ PF+ N
Sbjct: 503 KLDRVLLNPEYNSAYPHSYCIMDSGGCSDHLRGRFHLRSAIQKPKGPFKFTN 554
>At2g15510 putative non-LTR retroelement reverse transcriptase
Length = 1138
Score = 45.1 bits (105), Expect = 9e-06
Identities = 30/100 (30%), Positives = 47/100 (47%), Gaps = 4/100 (4%)
Query: 7 FCIAGDFNVVARSEERMGISDSIYGRSDMADFKAFLSNLSLIEPPAVGKKYTW--FRPNG 64
+C+ GDFNV+ + E+ G G S FK L ++E P+ G ++W R +
Sbjct: 97 WCMLGDFNVILHNGEKRG--GPRRGDSSFISFKDMLECCDMLELPSKGNPFSWGGKRNSL 154
Query: 65 QAASRLDRFLLSSDWCSCWPHGVQTVMARSLSDHCPVMFR 104
RLDR + +W +P Q + + SD PV+ R
Sbjct: 155 WIQCRLDRCFGNKNWFRHFPVSNQEFLDKRGSDRRPVLVR 194
>At2g05980 putative non-LTR retroelement reverse transcriptase
Length = 1352
Score = 45.1 bits (105), Expect = 9e-06
Identities = 32/114 (28%), Positives = 48/114 (42%), Gaps = 3/114 (2%)
Query: 9 IAGDFNVVARSEERMGISDSIYGRSDMADFKAFLSNLSLIEPPAVGKKYTWF--RPNGQA 66
I GDFN + E+ +S + M DF+ + SL + G +TW R G
Sbjct: 376 ILGDFNEILDGEDHSNFVNSPFIPQGMRDFQDVVRYCSLADMSYHGPVFTWCNKREEGLV 435
Query: 67 ASRLDRFLLSSDWCSCWPHGVQTVMARSLSDHCPVMFRSTDMNWGPR-PFRVLN 119
+LDR L++ W +P A +SDH + N P+ PF+ N
Sbjct: 436 NKKLDRVLVNEVWTRKFPAVYSIFEAGGISDHLRCRIKFASGNTQPKGPFKFNN 489
>At2g01550 putative non-LTR retroelement reverse transcriptase
Length = 1449
Score = 45.1 bits (105), Expect = 9e-06
Identities = 33/120 (27%), Positives = 54/120 (44%), Gaps = 12/120 (10%)
Query: 9 IAGDFNVVARSEERMGISDSIYGRSDMADFKAFLSNLSLIEPPAVGKKYTWF--RPNGQA 66
I GDFN + +E + D S M DF++ ++ S + + G +TW R N
Sbjct: 554 IFGDFNEILDMDEHSRMEDHPAVTSGMRDFQSLVNYCSFSDLASHGPLFTWCNKRDNDPI 613
Query: 67 ASRLDRFLLSSDWCSCWPHGVQTVMARSLSDH--CPVMFRSTDMNWGP-----RPFRVLN 119
+LDR +++ W +P A SDH C + + +MN G +PF+ +N
Sbjct: 614 WKKLDRVMVNEAWKMVYPQSYNVFEAGGCSDHLRCRI---NLNMNSGAQVRGNKPFKFVN 670
>At1g31100 hypothetical protein
Length = 1090
Score = 43.1 bits (100), Expect = 3e-05
Identities = 35/114 (30%), Positives = 50/114 (43%), Gaps = 8/114 (7%)
Query: 11 GDFN-VVARSEERMGISDSIYGRSDMADFKAFLSNLSLIEPPAVGKKYTWFRPNGQ--AA 67
GDFN V+ +E S ++ R M F+ L L + G +TW+ + A
Sbjct: 54 GDFNQVLCPAEHSQATSLNVNRR--MKVFRDCLFEAELCDLVFKGNTFTWWNKSATRPVA 111
Query: 68 SRLDRFLLSSDWCSCWPHGVQTVMARSLSDH--CPVMFRSTDMNWGPRPFRVLN 119
+LDR L++ WCS +P SDH C V+ M+ RPFR N
Sbjct: 112 KKLDRILVNESWCSRFPSAYAVFGEPDFSDHASCGVIINPL-MHREKRPFRFYN 164
>At1g25430 hypothetical protein
Length = 1213
Score = 42.7 bits (99), Expect = 5e-05
Identities = 31/115 (26%), Positives = 51/115 (43%), Gaps = 6/115 (5%)
Query: 9 IAGDFNVVARSEERMGISDSIYGRSDMADFKAFLSNLSLIEPPAVGKKYTWFRPNGQA-- 66
+ GDFN V +E S+ +M DF+ L L + G +TW+ +
Sbjct: 141 VLGDFNQVLNPQEHSN-PVSLNVDINMRDFRDCLLAAELSDLRYKGNTFTWWNKSHTTPV 199
Query: 67 ASRLDRFLLSSDWCSCWPHGVQTVMARSLSDH--CPVMFRSTDMNWGPRPFRVLN 119
A ++DR L++ W + +P + + SDH C V+ T + RPF+ N
Sbjct: 200 AKKIDRILVNDSWNALFPSSLGIFGSLDFSDHVSCGVVLEETSIK-AKRPFKFFN 253
>At2g16680 putative non-LTR retroelement reverse transcriptase
Length = 1319
Score = 41.2 bits (95), Expect = 1e-04
Identities = 26/100 (26%), Positives = 48/100 (48%), Gaps = 4/100 (4%)
Query: 5 SCFCIAGDFNVVARSEERMGISDSIYGRSDMADFKAFLSNLSLIEPPAVGKKYTW--FRP 62
S +C+ GDFN + ++ ++G + S FK L N + + + G +TW R
Sbjct: 92 SAWCMIGDFNDILSNDGKLGGPSRLI--SSFQPFKNMLLNCDMHQMGSSGNSFTWGGTRN 149
Query: 63 NGQAASRLDRFLLSSDWCSCWPHGVQTVMARSLSDHCPVM 102
+ +LDR +S+W + + + Q + + S H PV+
Sbjct: 150 DQWIQCKLDRCFGNSEWFTMFSNSHQWFLEKLGSHHRPVL 189
>At1g37050 putative non-LTR retroelement reverse transcriptase
Length = 695
Score = 41.2 bits (95), Expect = 1e-04
Identities = 29/117 (24%), Positives = 50/117 (41%), Gaps = 6/117 (5%)
Query: 9 IAGDFNVVARSEERMGISDSIYGRSDMADFKAFLSNLSLIEPPAVGKKYTWF--RPNGQA 66
I GDFN + ++ + + S M DF+ + +L + + G +TW R +G
Sbjct: 449 IFGDFNEILNMDKHSQLENQPVVTSRMRDFQDVIQYCTLSDMDSHGPLFTWSNKRDHGLI 508
Query: 67 ASRLDRFLLSSDWCSCWPHGVQTVMARSLSDH--CPVMFRSTDMNWG--PRPFRVLN 119
+LDR L++ W +P A +H C + + + G RPF +N
Sbjct: 509 MKKLDRVLMNDVWLQNYPMSYSVFEAGGFFNHLRCKINLTTVNEERGRERRPFNFVN 565
>At3g31420 hypothetical protein
Length = 1491
Score = 40.8 bits (94), Expect = 2e-04
Identities = 23/96 (23%), Positives = 43/96 (43%), Gaps = 4/96 (4%)
Query: 9 IAGDFNVVARSEERMGISDSIYGRSDMADFKAFLSNLSLIEPPAVGKKYTWFRPNGQ--A 66
I GDFN + + E+ G + DF+ + + + +VG +++W G
Sbjct: 314 IMGDFNEILSNREKRG--GRLRPERTFQDFRNMVRGCNFTDLKSVGDRFSWAGKRGDHHV 371
Query: 67 ASRLDRFLLSSDWCSCWPHGVQTVMARSLSDHCPVM 102
LDR + +++W + +P M SDH P++
Sbjct: 372 TCSLDRTMANNEWHTLFPESETVFMEYGESDHRPLV 407
>At2g01840 putative non-LTR retroelement reverse transcriptase
Length = 1715
Score = 40.0 bits (92), Expect = 3e-04
Identities = 26/96 (27%), Positives = 45/96 (46%), Gaps = 4/96 (4%)
Query: 9 IAGDFNVVARSEERMGISDSIYGRSDMADFKAFLSNLSLIEPPAVGKKYTWF--RPNGQA 66
+ GDFN + E+ G G + +F ++ ++ + + G Y+W R N
Sbjct: 495 MCGDFNEILNLNEKKGGRRRSIG--SLQNFTNMINCCNMKDLKSKGNPYSWVGKRQNETI 552
Query: 67 ASRLDRFLLSSDWCSCWPHGVQTVMARSLSDHCPVM 102
S LDR ++SDW + +P + + SDH PV+
Sbjct: 553 ESCLDRVFINSDWQASFPAFETEFLPIAGSDHAPVI 588
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.325 0.137 0.471
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,790,822
Number of Sequences: 26719
Number of extensions: 99715
Number of successful extensions: 334
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 27
Number of HSP's successfully gapped in prelim test: 15
Number of HSP's that attempted gapping in prelim test: 289
Number of HSP's gapped (non-prelim): 42
length of query: 124
length of database: 11,318,596
effective HSP length: 87
effective length of query: 37
effective length of database: 8,994,043
effective search space: 332779591
effective search space used: 332779591
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 53 (25.0 bits)
Lotus: description of TM0281b.2