BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0276.3
(333 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g14260 proline iminopeptidase 566 e-162
At3g61540 prolyl aminopeptidase-like protein 49 5e-06
At3g51000 epoxide hydrolase-like protein 42 5e-04
At4g12830 hydrolase like protein 41 0.001
At4g24160 unknown protein 38 0.009
At4g02340 unknown protein 38 0.009
At5g22460 putative protein 36 0.026
At3g03240 hypothetical protein 36 0.026
At5g53050 unknown protein 35 0.075
At4g37470 unknown protein 33 0.28
At2g26750 putative epoxide hydrolase 33 0.28
At3g12130 unknown protein 32 0.48
At3g54240 putative protein (fragment) 32 0.63
At2g26740 epoxide hydrolase (ATsEH) 31 0.82
At5g41900 unknown protein 30 1.4
At5g21950 unknown protein 30 1.4
At5g35630 glutamate-ammonia ligase (EC 6.3.1.2) precursor, chlor... 30 1.8
At2g40120 protein kinase like protein 30 1.8
At5g20520 unknown protein 30 2.4
At3g09690 unknown protein 29 3.1
>At2g14260 proline iminopeptidase
Length = 380
Score = 567 bits (1460), Expect = e-162
Identities = 264/316 (83%), Positives = 290/316 (91%), Gaps = 1/316 (0%)
Query: 12 RNLYPHIEPYSTGLLKVSDLHTIYWEQSGNPSGHPVVFLHGGPGGGTSPSNRRFFDPEFY 71
R LY IEPYS+G LKVSD+HT+YWEQSG P GHPVVFLHGGPGGGT+PSNRRFFDPEFY
Sbjct: 64 RTLYAPIEPYSSGNLKVSDVHTLYWEQSGKPDGHPVVFLHGGPGGGTAPSNRRFFDPEFY 123
Query: 72 RIILFDQRGAGKSTPHACLEHNTTWDLIDDIEKLREHLEIPEWQVFGGSWGSTLALAYSQ 131
RI+LFDQRGAGKSTPHACLE NTTWDL++DIEKLREHL+IPEW VFGGSWGSTLALAYSQ
Sbjct: 124 RIVLFDQRGAGKSTPHACLEENTTWDLVNDIEKLREHLKIPEWLVFGGSWGSTLALAYSQ 183
Query: 132 SHPDKVTGIILRGIFLLRKKEIDWFYEGGAAAIFPDAWEPFRDLIPENERG-CFVDAYKK 190
SHPDKVTG++LRGIFLLRKKEIDWFYEGGAAAI+PDAWE FRDLIPENERG VDAY K
Sbjct: 184 SHPDKVTGLVLRGIFLLRKKEIDWFYEGGAAAIYPDAWEEFRDLIPENERGSSLVDAYHK 243
Query: 191 RLNSDDIQTQFAAARAWTKWEMMTAHLIPNEDNVKKGDDDHFSLAFARIENHYFVNKGFF 250
RLNSDD++ Q+AAARAWTKWEMMTA+L PN +NV+K +DD FSLAFARIENHYFVNKGFF
Sbjct: 244 RLNSDDLEIQYAAARAWTKWEMMTAYLRPNLENVQKAEDDKFSLAFARIENHYFVNKGFF 303
Query: 251 SSDSYLLDGVDKIRHINTTIVQGRYDVCCPMMSAWDLHKAWPEADFRVVADAGHSANELG 310
SDS+LLD VDKIRHI TTIVQGRYDVCCPMMSAWDLHKAWPEA+ ++V DAGHSANE G
Sbjct: 304 PSDSHLLDNVDKIRHIKTTIVQGRYDVCCPMMSAWDLHKAWPEAELKIVYDAGHSANEPG 363
Query: 311 IAAELVAANEKLKNII 326
I+AELV ANEK+K ++
Sbjct: 364 ISAELVVANEKMKALM 379
>At3g61540 prolyl aminopeptidase-like protein
Length = 515
Score = 48.5 bits (114), Expect = 5e-06
Identities = 35/119 (29%), Positives = 54/119 (44%), Gaps = 21/119 (17%)
Query: 47 VVFLHGGPG-GGTSPSNRRFFDP---EFYRIILFDQRGAGKSTPHAC------------- 89
+++L GGPG G PS + E +R++L DQRG G STP C
Sbjct: 120 LLYLQGGPGFEGPRPSEASGWIQRACEEFRVVLLDQRGTGLSTPLTCSSMLQFKSAKELA 179
Query: 90 --LEHNTTWDLIDDIEKLREHL--EIPEWQVFGGSWGSTLALAYSQSHPDKVTGIILRG 144
L H +++ D E +R L + W + G S+G AL Y P+ + +++ G
Sbjct: 180 DYLVHFRADNIVKDAEFIRVRLVPKADPWTILGQSFGGFCALTYLSFAPEGLKQVLITG 238
>At3g51000 epoxide hydrolase-like protein
Length = 323
Score = 42.0 bits (97), Expect = 5e-04
Identities = 30/105 (28%), Positives = 44/105 (41%), Gaps = 1/105 (0%)
Query: 38 QSGNPSGHPVVFLHGGPGGGTSPSNR-RFFDPEFYRIILFDQRGAGKSTPHACLEHNTTW 96
+ G+ G V+ LHG P S ++ F Y ++ D RG G S E T
Sbjct: 21 EKGDEEGPLVLLLHGFPETWYSWRHQIDFLSSHGYHVVAPDLRGYGDSDSLPSHESYTVS 80
Query: 97 DLIDDIEKLREHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGII 141
L+ D+ L +H + V G WG+ + PD+V G I
Sbjct: 81 HLVADVIGLLDHYGTTQAFVAGHDWGAIIGWCLCLFRPDRVKGFI 125
>At4g12830 hydrolase like protein
Length = 393
Score = 40.8 bits (94), Expect = 0.001
Identities = 31/115 (26%), Positives = 50/115 (42%), Gaps = 2/115 (1%)
Query: 30 DLHTIYWEQSGNPSGHPVVFLHGGPGGGTSPSNRRFFDPEFYRIILFDQRGAGKS-TPHA 88
DL + +SG+ PV+ +HG P S + YR I FD G G S P A
Sbjct: 119 DLFRWFSVESGSVDSPPVILIHGFPSQAYSYRKTIPVLSKNYRAIAFDWLGFGFSDKPQA 178
Query: 89 CLEHNTTWD-LIDDIEKLREHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGIIL 142
N T D + +E + + + + + S + Y+++ PDK+ +IL
Sbjct: 179 GYGFNYTMDEFVSSLESFIDEVTTSKVSLVVQGYFSAAVVKYARNRPDKIKNLIL 233
>At4g24160 unknown protein
Length = 418
Score = 37.7 bits (86), Expect = 0.009
Identities = 25/78 (32%), Positives = 38/78 (48%), Gaps = 5/78 (6%)
Query: 71 YRIILFDQRGAGKST--PHAC--LEHNTTWDLIDDIEKLREHLEIPEWQVFGGSWGSTLA 126
+R+I DQ G G S+ C E W ID E+ R+ + + + G S+G +A
Sbjct: 147 FRVIAIDQLGWGGSSRPDFTCRSTEETEAW-FIDSFEEWRKAQNLSNFILLGHSFGGYVA 205
Query: 127 LAYSQSHPDKVTGIILRG 144
Y+ HP+ V +IL G
Sbjct: 206 AKYALKHPEHVQHLILVG 223
>At4g02340 unknown protein
Length = 324
Score = 37.7 bits (86), Expect = 0.009
Identities = 28/100 (28%), Positives = 44/100 (44%), Gaps = 1/100 (1%)
Query: 43 SGHPVVFLHGGPGGGTSPSNRRF-FDPEFYRIILFDQRGAGKSTPHACLEHNTTWDLIDD 101
SG ++F+HG P S ++ F YR I D RG G S E T ++ D
Sbjct: 23 SGPVILFVHGFPDLWYSWRHQLVSFAALGYRAIAPDLRGYGDSDAPPSRESYTILHIVGD 82
Query: 102 IEKLREHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGII 141
+ L + L + + G WG+ +A PD+V ++
Sbjct: 83 LVGLLDSLGVDRVFLVGHDWGAIVAWWLCMIRPDRVNALV 122
>At5g22460 putative protein
Length = 340
Score = 36.2 bits (82), Expect = 0.026
Identities = 30/134 (22%), Positives = 64/134 (47%), Gaps = 9/134 (6%)
Query: 15 YPHIEPYSTGLLKVSDLHTIYWEQSG---NPSGHPVVFLHGGPGGGTS--PSNRRFFDPE 69
+P+ P ++ +K+SD + + +SG + + + ++ +HG + P + +
Sbjct: 31 HPNGPPVTSPRIKLSDGRYLAYRESGVDRDNANYKIIVVHGFNSSKDTEFPIPKDVIEEL 90
Query: 70 FYRIILFDQRGAGKSTPHACLEHNTTWDLIDDIEKLREHLEI-PEWQVFGGSWGSTLALA 128
+ +D+ G G+S PH T DI++L + L+I P++ V G S G+ +
Sbjct: 91 GIYFVFYDRAGYGESDPH---PSRTVKSEAYDIQELADKLKIGPKFYVLGISLGAYSVYS 147
Query: 129 YSQSHPDKVTGIIL 142
+ P ++ G +L
Sbjct: 148 CLKYIPHRLAGAVL 161
>At3g03240 hypothetical protein
Length = 326
Score = 36.2 bits (82), Expect = 0.026
Identities = 32/121 (26%), Positives = 55/121 (45%), Gaps = 12/121 (9%)
Query: 26 LKVSDLHTIYWEQSGNPSG---HPVVFLHGGPGGGTSPSNRRFFDPEFYRIILFDQRGAG 82
+K++D + +++ G P + ++ LHG S D +LFD+ G G
Sbjct: 38 IKLNDGRYLAYKELGFPKDKAKNKIIILHG-----FGSSKLEMIDEFEIYFLLFDRAGYG 92
Query: 83 KSTPHACLEHNTTWDLIDDIEKLREHLEI-PEWQVFGGSWGSTLALAYSQSHPDKVTGII 141
+S PH T DIE+L + L+I P++ V G S G+ + P +++G
Sbjct: 93 ESDPHPSRTLKTD---TYDIEELADKLQIGPKFHVLGMSLGAYPVYGCLKYIPHRLSGAT 149
Query: 142 L 142
L
Sbjct: 150 L 150
>At5g53050 unknown protein
Length = 396
Score = 34.7 bits (78), Expect = 0.075
Identities = 20/79 (25%), Positives = 35/79 (43%)
Query: 67 DPEFYRIILFDQRGAGKSTPHACLEHNTTWDLIDDIEKLREHLEIPEWQVFGGSWGSTLA 126
D + FD RG G+S+ TT + +D L +HL + + G S G+ +A
Sbjct: 75 DDSGIEVCAFDNRGMGRSSVPTHKSEYTTTIMANDSISLLDHLGWKKAHIIGHSMGAMIA 134
Query: 127 LAYSQSHPDKVTGIILRGI 145
+ P++V + L +
Sbjct: 135 CKLAAMAPERVLSLALLNV 153
>At4g37470 unknown protein
Length = 270
Score = 32.7 bits (73), Expect = 0.28
Identities = 35/128 (27%), Positives = 57/128 (44%), Gaps = 19/128 (14%)
Query: 43 SGHPVVFLHGGPGGGTSPSNRRFFDPEF---YRIILFDQRGAGKSTP-------HACLEH 92
SG + L G G GT S + P YR++L+D GAG + P ++ LE
Sbjct: 15 SGEATIVL--GHGFGTDQSVWKHLVPHLVDDYRVVLYDNMGAGTTNPDYFDFDRYSNLE- 71
Query: 93 NTTWDLIDDIEKLREHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGIILRGIFLLRKKE 152
++DLI + E L+I G S + + + S + PD + I++ +
Sbjct: 72 GYSFDLI----AILEDLKIESCIFVGHSVSAMIGVLASLNRPDLFSKIVMISASPRYVND 127
Query: 153 IDWFYEGG 160
+D Y+GG
Sbjct: 128 VD--YQGG 133
>At2g26750 putative epoxide hydrolase
Length = 320
Score = 32.7 bits (73), Expect = 0.28
Identities = 21/73 (28%), Positives = 35/73 (47%), Gaps = 2/73 (2%)
Query: 71 YRIILFDQRGAGKSTPHACLEHNTTWDLIDDIEKLREHLEIPEWQVF--GGSWGSTLALA 128
YR + D RG G S A + T ++++ D+ + L + +VF G WG+ +A
Sbjct: 51 YRAVAPDLRGYGDSDAPAEISSFTCFNIVGDLVAVISTLIKEDKKVFVVGHDWGALIAWY 110
Query: 129 YSQSHPDKVTGII 141
PDKV ++
Sbjct: 111 LCLFRPDKVKALV 123
>At3g12130 unknown protein
Length = 248
Score = 32.0 bits (71), Expect = 0.48
Identities = 20/51 (39%), Positives = 24/51 (46%)
Query: 18 IEPYSTGLLKVSDLHTIYWEQSGNPSGHPVVFLHGGPGGGTSPSNRRFFDP 68
+E YSTGL S T ++ SG P G FLH PGG + S P
Sbjct: 28 MESYSTGLGSKSKPCTKFFSTSGCPFGENCHFLHYVPGGYNAVSQMTNMGP 78
>At3g54240 putative protein (fragment)
Length = 342
Score = 31.6 bits (70), Expect = 0.63
Identities = 20/79 (25%), Positives = 37/79 (46%), Gaps = 4/79 (5%)
Query: 65 FFDPEFYRIILFDQRGAGKSTPHACLEHNTTWDLIDDIEKLREHLEI-PEWQVFGGSWGS 123
F + ++ +D+ G G+S PH+ T L D+E+L + L++ ++ V G S G
Sbjct: 85 FLEKNGIYVVSYDRPGYGESDPHSSRNEKT---LAHDVEQLADQLQLGSKFYVVGYSMGG 141
Query: 124 TLALAYSQSHPDKVTGIIL 142
+ P ++ G L
Sbjct: 142 QAVWGVLKYIPHRLAGATL 160
>At2g26740 epoxide hydrolase (ATsEH)
Length = 321
Score = 31.2 bits (69), Expect = 0.82
Identities = 20/74 (27%), Positives = 34/74 (45%), Gaps = 3/74 (4%)
Query: 71 YRIILFDQRGAGKSTPHACLEHNTTWDLIDDIEKLREHLEIPEWQ---VFGGSWGSTLAL 127
YR + D RG G S A + T ++++ D+ + L E + V G WG+ +A
Sbjct: 51 YRAVAPDLRGYGDSDAPAEISSYTCFNIVGDLIAVISALTASEDEKVFVVGHDWGALIAW 110
Query: 128 AYSQSHPDKVTGII 141
PD+V ++
Sbjct: 111 YLCLFRPDRVKALV 124
>At5g41900 unknown protein
Length = 471
Score = 30.4 bits (67), Expect = 1.4
Identities = 32/114 (28%), Positives = 42/114 (36%), Gaps = 31/114 (27%)
Query: 47 VVFLHGGPGGGTSPSNRRFFDPEF-------YRIILFDQRGAGKSTPHACLEHNTTWDLI 99
VVF+HG + F P F YR I D G G+S
Sbjct: 193 VVFIHGFVSSSAFWTETLF--PNFSDSAKSNYRFIAVDLLGYGRSPKPN----------- 239
Query: 100 DDIEKLREHLEIPE-----------WQVFGGSWGSTLALAYSQSHPDKVTGIIL 142
D + LREHLE+ E + + S G LALA + HP + + L
Sbjct: 240 DSLYTLREHLEMIEKSVISKFKLKTFHIVAHSLGCILALALAVKHPGAIKSLTL 293
>At5g21950 unknown protein
Length = 220
Score = 30.4 bits (67), Expect = 1.4
Identities = 14/39 (35%), Positives = 23/39 (58%)
Query: 104 KLREHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGIIL 142
KL E LE+ + V G S+G +A ++ P+KV ++L
Sbjct: 113 KLMEKLEVERFSVVGTSYGGFVAYNMAKMFPEKVEKVVL 151
>At5g35630 glutamate-ammonia ligase (EC 6.3.1.2) precursor,
chloroplast (clone lambdaAtgsl1) (pir||S18600)
Length = 430
Score = 30.0 bits (66), Expect = 1.8
Identities = 27/117 (23%), Positives = 47/117 (40%), Gaps = 19/117 (16%)
Query: 102 IEK-LREHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGIILRGIFLLRKKEIDWFYEGG 160
IEK + + E+P+W G S G P + + +IL + R D F G
Sbjct: 98 IEKPVEDPSELPKWNYDGSSTGQA---------PGEDSEVILYPQAIFR----DPFRGGN 144
Query: 161 AAAIFPDAWEPFRDLIPENERGCFVDAYKKRLNSDD-----IQTQFAAARAWTKWEM 212
+ D W P + IP N+R + + + S + I+ ++ + KW +
Sbjct: 145 NILVICDTWTPAGEPIPTNKRAKAAEIFSNKKVSGEVPWFGIEQEYTLLQQNVKWPL 201
>At2g40120 protein kinase like protein
Length = 570
Score = 30.0 bits (66), Expect = 1.8
Identities = 17/53 (32%), Positives = 25/53 (47%), Gaps = 6/53 (11%)
Query: 184 FVDAYKKRLNSDDIQTQFAAARAWTKWEMMTAHLIPNEDNVKKGDDDHFSLAF 236
FV+ Y +S D Q+QF AR + +W H N + +D+ S AF
Sbjct: 93 FVNPYGDSSSSSDGQSQFGTARTYPEWSEFYLH------NETEDEDEFMSPAF 139
>At5g20520 unknown protein
Length = 308
Score = 29.6 bits (65), Expect = 2.4
Identities = 20/80 (25%), Positives = 35/80 (43%), Gaps = 11/80 (13%)
Query: 73 IILFDQRGAGKSTPHACLEHNTTWDLIDDIEKLREHL------EIPEWQVFGGSWGSTLA 126
+ + RG G S + + +I D + +HL + VFG S G +
Sbjct: 111 VFMLSYRGYGASEGYPSQQ-----GIIKDAQAALDHLSGRTDIDTSRIVVFGRSLGGAVG 165
Query: 127 LAYSQSHPDKVTGIILRGIF 146
++++PDKV+ +IL F
Sbjct: 166 AVLTKNNPDKVSALILENTF 185
>At3g09690 unknown protein
Length = 527
Score = 29.3 bits (64), Expect = 3.1
Identities = 19/71 (26%), Positives = 34/71 (47%), Gaps = 2/71 (2%)
Query: 72 RIILFDQRGAGKSTPHACLEHNTTWDLIDDIEKLREHLEIPEWQVFGGSWGSTLALAYSQ 131
R++ +D G G+S PH N + D I+ + ++ + G S GS A A +
Sbjct: 218 RLVSYDLPGFGESDPHRA--RNLSSSASDMIDLAAALGIVDKFWLLGYSSGSVHAWAAMR 275
Query: 132 SHPDKVTGIIL 142
PD++ G+ +
Sbjct: 276 YFPDQIAGVAM 286
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.320 0.138 0.439
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,560,383
Number of Sequences: 26719
Number of extensions: 408527
Number of successful extensions: 765
Number of sequences better than 10.0: 29
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 18
Number of HSP's that attempted gapping in prelim test: 752
Number of HSP's gapped (non-prelim): 29
length of query: 333
length of database: 11,318,596
effective HSP length: 100
effective length of query: 233
effective length of database: 8,646,696
effective search space: 2014680168
effective search space used: 2014680168
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0276.3