BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0269b.6
(141 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g18670 extensin-like protein 30 0.32
At3g15990 putative sulfate transporter 30 0.55
At2g45620 unknown protein 29 0.72
At3g56260 unknown protein 29 0.94
At1g66550 WRKY transcription factor 67 (WRKY67) 28 2.1
At1g49270 hypothetical protein 27 3.6
At2g06310 putative Athila retroelement ORF1 protein 27 4.7
At1g23090 putative sulphate transporter protein 27 4.7
At5g40490 ribonucleoprotein -like 26 6.1
At5g07750 putative protein 26 6.1
At5g06700 unknown protein 26 6.1
At4g02700 sulfate transporter protein 26 6.1
At3g61310 DNA-binding protein like 26 6.1
At3g51895 sulfate transporter (ATST1) 26 6.1
At1g71830 protein kinase, putative 26 6.1
At1g34210 putative protein 26 6.1
At1g05870 unknown protein 26 6.1
At3g26480 WD-repeat protein, putative 26 8.0
At2g41910 putative protein kinase 26 8.0
At1g78000 sulfate transporter (Sultr1;2) 26 8.0
>At4g18670 extensin-like protein
Length = 839
Score = 30.4 bits (67), Expect = 0.32
Identities = 19/52 (36%), Positives = 22/52 (41%), Gaps = 2/52 (3%)
Query: 2 GGPPFTP--PPPWGWSTGFITTPLTTGRLPSQHFDPALPKLFWFTPTFPTCP 51
G PP +P P P G TTP G PS P+ P P+ PT P
Sbjct: 492 GSPPSSPTTPTPGGSPPSSPTTPSPGGSPPSPSISPSPPITVPSPPSTPTSP 543
>At3g15990 putative sulfate transporter
Length = 653
Score = 29.6 bits (65), Expect = 0.55
Identities = 16/52 (30%), Positives = 21/52 (39%), Gaps = 10/52 (19%)
Query: 12 WGWST-----GFITTPLTTGRLPSQHFDPALPKLFWFTPTFPTCPTVAEQFL 58
W W T GF++ LTT +H PKLFW + P + L
Sbjct: 250 WSWETIVMGIGFLSILLTT-----RHISMRKPKLFWISAASPLASVIISTLL 296
>At2g45620 unknown protein
Length = 764
Score = 29.3 bits (64), Expect = 0.72
Identities = 25/84 (29%), Positives = 30/84 (34%), Gaps = 20/84 (23%)
Query: 3 GPPFTPP-PPWGWSTGFITTPLTTGRLPSQHFDPALPKLF----------WFTPTFPTCP 51
GP P PP W + G PS H P+ P F P FP P
Sbjct: 52 GPTVNNPFPPSNWQSN--------GHRPSNHNPPSWPLAFSPPHNLSPNFLGFPQFPPSP 103
Query: 52 TVAEQFLDIKRTSPEGNFNVADFP 75
QF +R SPE + + FP
Sbjct: 104 FTTNQFDGNQRVSPEDAYRLG-FP 126
>At3g56260 unknown protein
Length = 197
Score = 28.9 bits (63), Expect = 0.94
Identities = 25/89 (28%), Positives = 44/89 (49%), Gaps = 19/89 (21%)
Query: 5 PFTPPPPWGWSTGFITTPLTTGRLPSQHFDPALPKLFWFTPTFPTCPTVAEQFLDIKRTS 64
P TPPP + +S+G ++TP T ++ S+ L K F+ F D++R S
Sbjct: 48 PLTPPPSY-YSSGILSTPRTQSKVRSK-----LSKFLSFS-----------MFSDLRR-S 89
Query: 65 PEGNFNVADFPSFAISFATAPAALANFPP 93
G+ A SF+ S ++ ++ ++ PP
Sbjct: 90 NHGSKKTAS-SSFSWSSTSSSSSFSSSPP 117
>At1g66550 WRKY transcription factor 67 (WRKY67)
Length = 254
Score = 27.7 bits (60), Expect = 2.1
Identities = 20/73 (27%), Positives = 30/73 (40%), Gaps = 8/73 (10%)
Query: 33 FDPALPKLFWFTPTFPTCPTVAEQFLDIKRTSPEGNFNV-ADFPSFAISFATAPAALANF 91
FD +P+ P P T+ Q + ++ S E N D + + P + F
Sbjct: 184 FDQVVPE-----PIMPQLTTIDHQVITVEENSAEHIMNQECDINDYLVD--DDPFWASQF 236
Query: 92 PPFPNVITHFFIN 104
PPFP+ T F N
Sbjct: 237 PPFPSSDTMFLEN 249
>At1g49270 hypothetical protein
Length = 699
Score = 26.9 bits (58), Expect = 3.6
Identities = 25/100 (25%), Positives = 35/100 (35%), Gaps = 26/100 (26%)
Query: 8 PPPPWGWSTGFITTPLTTGRLPSQHFDPALPKLFWFTPTFPTCPTVAEQFLDIKRTSPEG 67
PPPP T ++++P P P W P P G
Sbjct: 251 PPPPGSMGTNWVSSP------------PPPPPGNWQPMPSPPAPV-------------SG 285
Query: 68 NFNVADFPSFAISFATAPAALANFPPFPNVITHFFINRTF 107
NV + +F++ P A + PP P+V F N TF
Sbjct: 286 GANVIQSGEMSSNFSSGPYAPSLPPPHPSVALG-FNNSTF 324
>At2g06310 putative Athila retroelement ORF1 protein
Length = 733
Score = 26.6 bits (57), Expect = 4.7
Identities = 16/47 (34%), Positives = 21/47 (44%), Gaps = 5/47 (10%)
Query: 45 PTFPTCPTVAEQFLDIK-----RTSPEGNFNVADFPSFAISFATAPA 86
PT PT PT A+ L +K + +GN+ P FA PA
Sbjct: 241 PTTPTSPTAAQTLLIVKIKCILHNNNKGNYQPQPPPGFAPQQNQGPA 287
>At1g23090 putative sulphate transporter protein
Length = 631
Score = 26.6 bits (57), Expect = 4.7
Identities = 12/47 (25%), Positives = 17/47 (35%)
Query: 12 WGWSTGFITTPLTTGRLPSQHFDPALPKLFWFTPTFPTCPTVAEQFL 58
W W T + L ++H PKLFW + P + L
Sbjct: 227 WSWQTIVMGVCFLLFLLSTRHLSMKKPKLFWVSAGAPLLSVIVSTLL 273
>At5g40490 ribonucleoprotein -like
Length = 423
Score = 26.2 bits (56), Expect = 6.1
Identities = 16/60 (26%), Positives = 29/60 (47%), Gaps = 5/60 (8%)
Query: 58 LDIKRTSPEGNFNVADFPSFAISFATAPAALANFPPFPNVITHFFINRTFYKRHKTKRDN 117
++IKRT P G+ + DF + I P+++ + + FF+ K H+ RD+
Sbjct: 111 VEIKRTIPRGSMSSNDFKTKKIFVGGIPSSVDD-----DEFKEFFMQFGELKEHQIMRDH 165
>At5g07750 putative protein
Length = 1289
Score = 26.2 bits (56), Expect = 6.1
Identities = 15/35 (42%), Positives = 19/35 (53%), Gaps = 2/35 (5%)
Query: 4 PPFTPPPPWGWSTGFITTPLTTGRLPSQHFDPALP 38
PP PPPP +S G ++ L T +LPS P P
Sbjct: 764 PP--PPPPAYYSVGQKSSDLQTSQLPSPPPPPPPP 796
>At5g06700 unknown protein
Length = 608
Score = 26.2 bits (56), Expect = 6.1
Identities = 14/39 (35%), Positives = 21/39 (52%)
Query: 61 KRTSPEGNFNVADFPSFAISFATAPAALANFPPFPNVIT 99
K+TS V F SF + FA +P+ ++ P F N+ T
Sbjct: 34 KKTSTFAYAFVITFVSFTLFFAFSPSPNSSSPWFSNIFT 72
>At4g02700 sulfate transporter protein
Length = 646
Score = 26.2 bits (56), Expect = 6.1
Identities = 11/43 (25%), Positives = 18/43 (41%)
Query: 6 FTPPPPWGWSTGFITTPLTTGRLPSQHFDPALPKLFWFTPTFP 48
F+ W W +G + L +++ PKLFW + P
Sbjct: 228 FSQSHMWRWESGVLGCCFLIFLLTTKYISKKRPKLFWISAMSP 270
>At3g61310 DNA-binding protein like
Length = 354
Score = 26.2 bits (56), Expect = 6.1
Identities = 12/29 (41%), Positives = 15/29 (51%)
Query: 2 GGPPFTPPPPWGWSTGFITTPLTTGRLPS 30
G P P P G STGF++ PL P+
Sbjct: 52 GSNPNPNPNPGGVSTGFVSPPLPVDSSPA 80
>At3g51895 sulfate transporter (ATST1)
Length = 658
Score = 26.2 bits (56), Expect = 6.1
Identities = 12/53 (22%), Positives = 19/53 (35%)
Query: 6 FTPPPPWGWSTGFITTPLTTGRLPSQHFDPALPKLFWFTPTFPTCPTVAEQFL 58
F+ W W +G + L +++F PK FW P + L
Sbjct: 237 FSQTHEWRWESGVLGCGFLFFLLSTRYFSIKKPKFFWVAAMAPLTSVILGSLL 289
>At1g71830 protein kinase, putative
Length = 625
Score = 26.2 bits (56), Expect = 6.1
Identities = 8/11 (72%), Positives = 10/11 (90%)
Query: 2 GGPPFTPPPPW 12
G PPF+PPPP+
Sbjct: 212 GSPPFSPPPPF 222
>At1g34210 putative protein
Length = 628
Score = 26.2 bits (56), Expect = 6.1
Identities = 8/11 (72%), Positives = 10/11 (90%)
Query: 2 GGPPFTPPPPW 12
G PPF+PPPP+
Sbjct: 215 GSPPFSPPPPF 225
>At1g05870 unknown protein
Length = 189
Score = 26.2 bits (56), Expect = 6.1
Identities = 14/47 (29%), Positives = 24/47 (50%)
Query: 37 LPKLFWFTPTFPTCPTVAEQFLDIKRTSPEGNFNVADFPSFAISFAT 83
+P+L P C +++++FLD K+ +V D PS + AT
Sbjct: 107 IPELCNTLPALELCYSMSQKFLDDKQNKSPETSSVEDCPSPPLVTAT 153
>At3g26480 WD-repeat protein, putative
Length = 764
Score = 25.8 bits (55), Expect = 8.0
Identities = 9/16 (56%), Positives = 12/16 (74%)
Query: 90 NFPPFPNVITHFFINR 105
NFPPFP++ FF +R
Sbjct: 731 NFPPFPDLCASFFSSR 746
>At2g41910 putative protein kinase
Length = 373
Score = 25.8 bits (55), Expect = 8.0
Identities = 15/47 (31%), Positives = 23/47 (48%), Gaps = 2/47 (4%)
Query: 14 WSTGFITTPLTTGRLPSQHFDPALPKL-FWFTPTFPT-CPTVAEQFL 58
WS G + + TG+ P H + L L W+ P P+ P A+ F+
Sbjct: 207 WSLGCVVLEMYTGKKPWWHNNYDLKDLKNWYAPMIPSDLPCDAKHFI 253
>At1g78000 sulfate transporter (Sultr1;2)
Length = 653
Score = 25.8 bits (55), Expect = 8.0
Identities = 16/57 (28%), Positives = 20/57 (35%), Gaps = 1/57 (1%)
Query: 12 WGWSTGFITTPLTTGRLPSQHFDPALPKLFWFTPTFPTCPTVAEQ-FLDIKRTSPEG 67
W W T I T L S+ KLFW P + F+ I R +G
Sbjct: 252 WNWQTILIGASFLTFLLTSKIIGKKSKKLFWVPAIAPLISVIVSTFFVYITRADKQG 308
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.329 0.144 0.500
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,895,220
Number of Sequences: 26719
Number of extensions: 174374
Number of successful extensions: 575
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 531
Number of HSP's gapped (non-prelim): 55
length of query: 141
length of database: 11,318,596
effective HSP length: 89
effective length of query: 52
effective length of database: 8,940,605
effective search space: 464911460
effective search space used: 464911460
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0269b.6