BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0269a.4
(70 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g17730 unknown protein 88 9e-19
At1g73030 unknown protein 87 2e-18
At1g56660 hypothetical protein 33 0.026
At3g55340 putative protein 28 0.84
At3g17510 CBL-interacting protein kinase 1 (CIPK1) 28 1.1
At3g55950 receptor kinase - like protein 27 1.4
At2g43990 putative protein 27 1.9
At4g14480 kinase like protein 27 2.4
At3g28510 hypothetical protein 27 2.4
At3g47790 ABC-type transport protein-like protein 26 3.2
At3g28040 unknown protein 26 3.2
At3g04990 hypothetical protein 26 3.2
At2g41330 unknown protein 26 3.2
At5g37620 putative protein 26 4.2
At3g25490 wall-associated serine/threonine kinase, putative 26 4.2
At2g18300 putative bHLH transcription factor (bHLH064) 26 4.2
At5g09300 branched-chain alpha keto-acid dehydrogenase E1 alpha ... 25 5.4
At5g04670 putative protein 25 7.1
At4g36700 globulin-like protein 25 7.1
At2g22560 hypothetical protein 25 7.1
>At1g17730 unknown protein
Length = 203
Score = 87.8 bits (216), Expect = 9e-19
Identities = 44/69 (63%), Positives = 50/69 (71%)
Query: 1 MNRIMELEFTSKPLQEKPRKCEKQEKLEKLKVKSTTEKGNMGNIHIYDENTIRKHTEQMN 60
MN+I EL+FTSK LQ + RKCEK+E+ EKLKVK EKGNM IY EN IRK +EQMN
Sbjct: 8 MNQIFELKFTSKSLQRQARKCEKEERSEKLKVKKAIEKGNMDGARIYAENAIRKRSEQMN 67
Query: 61 YLHPSLYLD 69
YL S LD
Sbjct: 68 YLRLSSRLD 76
>At1g73030 unknown protein
Length = 203
Score = 87.0 bits (214), Expect = 2e-18
Identities = 43/69 (62%), Positives = 50/69 (72%)
Query: 1 MNRIMELEFTSKPLQEKPRKCEKQEKLEKLKVKSTTEKGNMGNIHIYDENTIRKHTEQMN 60
MN+I +L+FTSK LQ + RKCEK+EK EKLKVK EKGNM IY EN IRK +EQMN
Sbjct: 8 MNQIFDLKFTSKSLQRQSRKCEKEEKAEKLKVKKAIEKGNMDGARIYAENAIRKRSEQMN 67
Query: 61 YLHPSLYLD 69
YL + LD
Sbjct: 68 YLRLASRLD 76
>At1g56660 hypothetical protein
Length = 522
Score = 33.1 bits (74), Expect = 0.026
Identities = 19/50 (38%), Positives = 27/50 (54%), Gaps = 1/50 (2%)
Query: 8 EFTSKPLQEKPRKCEKQEKLEKLKVKSTTEKGNMGNIHIYDENTIRKHTE 57
E KP +EK +K E + +K KVK EKG G++ DE ++H E
Sbjct: 189 EKKKKPKKEKKQKEESKSNEDK-KVKGKKEKGEKGDLEKEDEEKKKEHDE 237
>At3g55340 putative protein
Length = 597
Score = 28.1 bits (61), Expect = 0.84
Identities = 11/30 (36%), Positives = 20/30 (66%)
Query: 15 QEKPRKCEKQEKLEKLKVKSTTEKGNMGNI 44
++KP+K + ++K +K KV T +K GN+
Sbjct: 108 EQKPQKKKNKKKKKKRKVNKTPKKAEEGNV 137
>At3g17510 CBL-interacting protein kinase 1 (CIPK1)
Length = 444
Score = 27.7 bits (60), Expect = 1.1
Identities = 15/55 (27%), Positives = 29/55 (52%), Gaps = 1/55 (1%)
Query: 15 QEKPRKCEKQEKLEKLKVKSTTEKGNMGNIHIYDENTIRKHTEQMNYLHPSLYLD 69
QE+ +K EK +L K ++ T +GN G + + ++T+ H+ + + S D
Sbjct: 5 QEEEKKAEKGMRLGKYELGRTLGEGNFGKVK-FAKDTVSGHSFAVKIIDKSRIAD 58
>At3g55950 receptor kinase - like protein
Length = 814
Score = 27.3 bits (59), Expect = 1.4
Identities = 14/40 (35%), Positives = 25/40 (62%), Gaps = 3/40 (7%)
Query: 21 CEKQEKLEKLKVKSTTEKGNMGNIHIYDENTIRKHTEQMN 60
CE++E EKL V + G + + H++D+N + KH+ +N
Sbjct: 564 CEERE--EKLLVYDYMKNGALYD-HLHDKNNVEKHSSLIN 600
>At2g43990 putative protein
Length = 632
Score = 26.9 bits (58), Expect = 1.9
Identities = 15/32 (46%), Positives = 21/32 (64%), Gaps = 1/32 (3%)
Query: 11 SKPLQEKPRKCEKQEKLEKLKVKST-TEKGNM 41
S P+ KP +K LE++K+KST TEK N+
Sbjct: 107 SAPVVSKPLIRKKPSSLEEVKLKSTLTEKPNL 138
>At4g14480 kinase like protein
Length = 487
Score = 26.6 bits (57), Expect = 2.4
Identities = 14/32 (43%), Positives = 20/32 (61%)
Query: 7 LEFTSKPLQEKPRKCEKQEKLEKLKVKSTTEK 38
+E S +EK R+ E E +EKLK++ TEK
Sbjct: 443 IEALSGDKEEKSREEELLEMVEKLKIELETEK 474
>At3g28510 hypothetical protein
Length = 530
Score = 26.6 bits (57), Expect = 2.4
Identities = 13/27 (48%), Positives = 18/27 (66%), Gaps = 2/27 (7%)
Query: 15 QEKPRKCEKQEKLEKLKVKSTTEKGNM 41
+EK +K E+ EK EK+K K E GN+
Sbjct: 490 EEKKKKTEEDEKKEKVKAKE--ENGNV 514
>At3g47790 ABC-type transport protein-like protein
Length = 727
Score = 26.2 bits (56), Expect = 3.2
Identities = 11/28 (39%), Positives = 18/28 (64%)
Query: 16 EKPRKCEKQEKLEKLKVKSTTEKGNMGN 43
EKP C ++EK+E+ +KST + + N
Sbjct: 439 EKPDVCREREKVEQCLLKSTRDSAVLCN 466
>At3g28040 unknown protein
Length = 997
Score = 26.2 bits (56), Expect = 3.2
Identities = 18/65 (27%), Positives = 32/65 (48%), Gaps = 5/65 (7%)
Query: 2 NRIMELEFTSKPLQEKPRKCEKQEKLEKLKVKSTTEKGNMGNIHIYDENTIRKHTEQMNY 61
+R++EL L K + +KL++LKV S + GNI+ N H ++++
Sbjct: 58 SRVIELSLDGLALTGKINR--GIQKLQRLKVLSLSNNNFTGNINALSNN---NHLQKLDL 112
Query: 62 LHPSL 66
H +L
Sbjct: 113 SHNNL 117
>At3g04990 hypothetical protein
Length = 227
Score = 26.2 bits (56), Expect = 3.2
Identities = 13/34 (38%), Positives = 19/34 (55%)
Query: 6 ELEFTSKPLQEKPRKCEKQEKLEKLKVKSTTEKG 39
EL T +QE ++ EK+ + +LK K EKG
Sbjct: 143 ELSMTVDQIQESGKQLEKKSREVELKDKEIEEKG 176
>At2g41330 unknown protein
Length = 402
Score = 26.2 bits (56), Expect = 3.2
Identities = 13/30 (43%), Positives = 19/30 (63%)
Query: 10 TSKPLQEKPRKCEKQEKLEKLKVKSTTEKG 39
TS+ QEKPR EK++ K+ V T+ +G
Sbjct: 236 TSEKDQEKPRLLEKEDNENKIVVYFTSLRG 265
>At5g37620 putative protein
Length = 652
Score = 25.8 bits (55), Expect = 4.2
Identities = 11/42 (26%), Positives = 24/42 (56%), Gaps = 1/42 (2%)
Query: 20 KCEKQEKL-EKLKVKSTTEKGNMGNIHIYDENTIRKHTEQMN 60
+C +E + E ++++ T E+ +G + D+NTI+ + N
Sbjct: 344 RCATREDVWEMVELEGTPEEDEIGPFEVIDDNTIKHFSHDHN 385
>At3g25490 wall-associated serine/threonine kinase, putative
Length = 433
Score = 25.8 bits (55), Expect = 4.2
Identities = 17/61 (27%), Positives = 29/61 (46%), Gaps = 6/61 (9%)
Query: 7 LEFTSKPLQEKPRKCEKQEKLEKLKVKST----TEKGNMGNIHIYDENTI--RKHTEQMN 60
+E T +E+PR E +LE L+ K+T ++ N+H+ N + + HT
Sbjct: 358 VECTRLKGEERPRMIEVAAELETLRAKTTKHNWLDQYPEENVHLLGSNIVSAQGHTSSRG 417
Query: 61 Y 61
Y
Sbjct: 418 Y 418
>At2g18300 putative bHLH transcription factor (bHLH064)
Length = 320
Score = 25.8 bits (55), Expect = 4.2
Identities = 21/71 (29%), Positives = 36/71 (50%), Gaps = 13/71 (18%)
Query: 3 RIMELEFTSKPLQEKPRKCEKQEKLEKLKVKSTTE-----KGNMGNIHIYDENTIR---- 53
R + L+ KP + K R+ +K EK K+KV++ TE K NMGN + +
Sbjct: 100 RAVALKNKRKP-EVKTREEQKTEK--KIKVEAETESSMKGKSNMGNTEASSDTSKETSKG 156
Query: 54 -KHTEQMNYLH 63
++++Y+H
Sbjct: 157 ASENQKLDYIH 167
>At5g09300 branched-chain alpha keto-acid dehydrogenase E1 alpha
subunit-like protein
Length = 472
Score = 25.4 bits (54), Expect = 5.4
Identities = 13/28 (46%), Positives = 18/28 (63%), Gaps = 2/28 (7%)
Query: 23 KQEKLEKLKVKSTTEKGNMGNI--HIYD 48
K+E LE L+V TEK N+ N+ +YD
Sbjct: 415 KKEMLEALRVAEKTEKPNLQNMFSDVYD 442
>At5g04670 putative protein
Length = 766
Score = 25.0 bits (53), Expect = 7.1
Identities = 14/53 (26%), Positives = 27/53 (50%), Gaps = 3/53 (5%)
Query: 2 NRIMELEFTSKPLQEKPRKCEKQEKLEKLKVKSTTEKGNMGNIHIYDENTIRK 54
N++ + S+P K EK++K++ V T++ + N + DE T+ K
Sbjct: 64 NKVSSGKSNSQPCSPKQVSSEKEDKVDDFPV---TKRRKVRNEGVGDEKTVDK 113
>At4g36700 globulin-like protein
Length = 499
Score = 25.0 bits (53), Expect = 7.1
Identities = 13/33 (39%), Positives = 20/33 (60%)
Query: 3 RIMELEFTSKPLQEKPRKCEKQEKLEKLKVKST 35
R+++L S L + +K EK+EK EK+K T
Sbjct: 209 RLLKLFAGSADLVDNKKKKEKKEKKEKVKKAKT 241
>At2g22560 hypothetical protein
Length = 891
Score = 25.0 bits (53), Expect = 7.1
Identities = 14/42 (33%), Positives = 24/42 (56%), Gaps = 1/42 (2%)
Query: 14 LQEKPRKCEKQEKLEKLKVKSTTEK-GNMGNIHIYDENTIRK 54
LQEK K ++ + E +K+K + EK +M + + DE+ K
Sbjct: 206 LQEKQEKSYEEAREEHVKIKESKEKLRSMASQFLGDESVFAK 247
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.311 0.130 0.362
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,625,421
Number of Sequences: 26719
Number of extensions: 56096
Number of successful extensions: 218
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 18
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 196
Number of HSP's gapped (non-prelim): 29
length of query: 70
length of database: 11,318,596
effective HSP length: 46
effective length of query: 24
effective length of database: 10,089,522
effective search space: 242148528
effective search space used: 242148528
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 52 (24.6 bits)
Lotus: description of TM0269a.4