BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0262.4
(180 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g14400 putative retroelement pol polyprotein 75 2e-14
At4g03770 hypothetical protein 70 5e-13
At3g31520 hypothetical protein 64 4e-11
At1g37180 hypothetical protein 62 2e-10
At1g41780 hypothetical protein 60 6e-10
At1g20390 hypothetical protein 60 6e-10
At2g15100 putative retroelement pol polyprotein 55 2e-08
At2g12870 putative retroelement pol polyprotein 54 5e-08
At2g15410 putative retroelement pol polyprotein 51 3e-07
At1g37060 Athila retroelment ORF 1, putative 44 4e-05
At3g32290 hypothetical protein 44 6e-05
At1g41690 hypothetical protein 42 2e-04
At2g07480 F9A16.15 40 7e-04
At3g30655 hypothetical protein, 3' partial 40 9e-04
At4g20490 putative protein 38 0.003
At1g34080 putative protein 37 0.004
At4g07640 putative athila transposon protein 36 0.013
At3g32890 Athila ORF 1, putative 36 0.013
At4g07780 putative athila transposon protein 35 0.022
At2g13260 putative Athila retroelement ORF1 protein 35 0.022
>At2g14400 putative retroelement pol polyprotein
Length = 1466
Score = 74.7 bits (182), Expect = 2e-14
Identities = 46/146 (31%), Positives = 78/146 (52%), Gaps = 5/146 (3%)
Query: 7 PFSAVVREVAIPDNMKSLALEAYSGGTDPKDHLLYFSM---KMVISAASDAVK-CRMFPS 62
PF+ + + I D+ K L LE+Y+G DPK +L F + ++ ++ A + V+ C++F
Sbjct: 144 PFTPQITSLRIRDSRK-LNLESYNGLEDPKGYLAAFLIAAGRVDLNEADEDVRYCKLFSE 202
Query: 63 MFKGTAMAWFTTLPLGSIVKFRDFSSKFLIQFSASKIKQVTIDDLYNVRQLERETLKQYV 122
G A+ WFT L GSI F + S FL Q+S K ++ DL+N+ Q ETL+ ++
Sbjct: 203 NLCGQALMWFTQLEPGSISNFNELSVVFLKQYSILMDKSISDTDLWNLSQGPNETLRAFI 262
Query: 123 KRYSAASVKIEELEP*ACARAFKDGL 148
++ K+ + + A + GL
Sbjct: 263 TKFKYVLSKLSRISQQSALSALRKGL 288
>At4g03770 hypothetical protein
Length = 464
Score = 70.5 bits (171), Expect = 5e-13
Identities = 48/166 (28%), Positives = 83/166 (49%), Gaps = 7/166 (4%)
Query: 7 PFSAVVREVAIPDNMKSLALEAYSGGTDPKDHLLYFSMKMVISA----ASDAVKCRMFPS 62
PF+ ++ I D K L +E ++G +DPK HL F + + + DA C +F
Sbjct: 218 PFTHMISNAIISDPGK-LRIEYFNGSSDPKGHLKLFIISVARAKFRPEERDAGLCHLFVE 276
Query: 63 MFKGTAMAWFTTLPLGSIVKFRDFSSKFLIQFSASKIKQVTIDDLYNVRQLERETLKQYV 122
KG A+ WFT L S+ F++ S+ FL Q+S + DL+++ Q E L+ ++
Sbjct: 277 HLKGPALNWFTRLKGNSVDSFQELSTLFLKQYSVLIDPSTSDADLWSLSQQPNEPLRDFL 336
Query: 123 KRYSAASVKIEELEP*ACARAFKDGL-LPGKLNNK-LSRKLARSMT 166
++ + K+E + A K L L +L+ K L+R++ +T
Sbjct: 337 AKFRSTLAKVEGINDLAALSTLKKALCLRARLSAKFLAREIGGGLT 382
>At3g31520 hypothetical protein
Length = 528
Score = 63.9 bits (154), Expect = 4e-11
Identities = 44/158 (27%), Positives = 74/158 (45%), Gaps = 5/158 (3%)
Query: 7 PFSAVVREVAIPDNMKSLALEAYSGGTDPKDHLLYFSMKMVISA----ASDAVKCRMFPS 62
PF+ + I D K L +E ++G +DPK HL F + + + DA C +F
Sbjct: 226 PFTHRISNAIISDPGK-LRIEYFNGSSDPKRHLKSFIISVARTKFRPEERDAGLCHLFVE 284
Query: 63 MFKGTAMAWFTTLPLGSIVKFRDFSSKFLIQFSASKIKQVTIDDLYNVRQLERETLKQYV 122
KG A+ WF+ L S+ F++ S+ FL Q+S + DL+++ Q E L+ ++
Sbjct: 285 HLKGPALCWFSRLEGNSMDSFQELSTLFLKQYSVLIDLGTSDADLWSLSQQPNEPLRDFL 344
Query: 123 KRYSAASVKIEELEP*ACARAFKDGLLPGKLNNKLSRK 160
+ + K+E + A A K L K + + K
Sbjct: 345 AKLRSTLAKVEGINDVAALSALKKALWVLKAGHSFTMK 382
>At1g37180 hypothetical protein
Length = 661
Score = 62.0 bits (149), Expect = 2e-10
Identities = 40/145 (27%), Positives = 67/145 (45%), Gaps = 5/145 (3%)
Query: 7 PFSAVVREVAIPDNMKSLALEAYSGGTDPKDHLLYFSMKM----VISAASDAVKCRMFPS 62
PF+ + ++ I + + L Y+G DP +HL F + + + DA C++F
Sbjct: 298 PFTPRISKLRIRE-FRDFKLPVYNGKGDPNEHLTSFQVIVRRVPLEPYEEDAGLCKLFSE 356
Query: 63 MFKGTAMAWFTTLPLGSIVKFRDFSSKFLIQFSASKIKQVTIDDLYNVRQLERETLKQYV 122
G A+ WFT L GSI F+ S+ F+ Q+ +T L+N+ Q + L+ Y+
Sbjct: 357 NLSGPALTWFTQLEEGSIDNFKQLSTAFIKQYGYFIKSDITEAHLWNLSQSADDPLRTYI 416
Query: 123 KRYSAASVKIEELEP*ACARAFKDG 147
+ V+I L A K+G
Sbjct: 417 IVFKEIMVQIPSLSDSTAQFALKNG 441
>At1g41780 hypothetical protein
Length = 246
Score = 60.1 bits (144), Expect = 6e-10
Identities = 37/123 (30%), Positives = 61/123 (49%), Gaps = 5/123 (4%)
Query: 7 PFSAVVREVAIPDNMKSLALEAYSGGTDPKDHLLYFSMKMVISA----ASDAVKCRMFPS 62
PF+ + I D K L +E +SG +DPK HL F + + + SDA C +F
Sbjct: 125 PFTRKISNAIISDPEK-LRIEYFSGSSDPKGHLKSFKISVARAKFKPEESDAGLCHLFVE 183
Query: 63 MFKGTAMAWFTTLPLGSIVKFRDFSSKFLIQFSASKIKQVTIDDLYNVRQLERETLKQYV 122
KG + WF+ L S+ F++ S+ FL Q+S + DL+++ Q E L ++
Sbjct: 184 HLKGPVLDWFSRLEGNSVDSFQELSTLFLKQYSVLIDPGTSDVDLWSLSQQPNEPLSDFL 243
Query: 123 KRY 125
++
Sbjct: 244 TKF 246
>At1g20390 hypothetical protein
Length = 1791
Score = 60.1 bits (144), Expect = 6e-10
Identities = 38/130 (29%), Positives = 66/130 (50%), Gaps = 5/130 (3%)
Query: 7 PFSAVVREVAIPDNMKSLALEAYSGGTDPKDHLLYFSMKMVISAAS----DAVKCRMFPS 62
PF+ + V+I K + LE+Y+G DPK+ L F++ + + + DA +C++F
Sbjct: 156 PFTRRITNVSIRGAQK-IKLESYNGRNDPKEFLTSFNVAINRAELTIDNFDAGRCQIFIE 214
Query: 63 MFKGTAMAWFTTLPLGSIVKFRDFSSKFLIQFSASKIKQVTIDDLYNVRQLERETLKQYV 122
G A WF+ L SI F +S FL ++ Q + DL+++ Q +E+L+ +V
Sbjct: 215 HLTGPAHNWFSRLKPNSIDSFHQLTSSFLKHYAPLIENQTSNADLWSISQGAKESLRSFV 274
Query: 123 KRYSAASVKI 132
R+ I
Sbjct: 275 DRFKLVVTNI 284
>At2g15100 putative retroelement pol polyprotein
Length = 1329
Score = 55.5 bits (132), Expect = 2e-08
Identities = 41/141 (29%), Positives = 62/141 (43%), Gaps = 5/141 (3%)
Query: 7 PFSAVVREVAIPDNMKSLALEAYSGGTDPKDHLLYFSMKM----VISAASDAVKCRMFPS 62
PF+ + ++ I + + L Y+G D K+HL F + + DA C++F
Sbjct: 157 PFTPRISKLRIRE-FRDFKLPVYNGKGDLKEHLTSFQVIAGRVPLEPHEEDAGLCKLFSE 215
Query: 63 MFKGTAMAWFTTLPLGSIVKFRDFSSKFLIQFSASKIKQVTIDDLYNVRQLERETLKQYV 122
G A+ WFT L GSI F+ S+ F+ Q+ +T L+N Q E L+ Y+
Sbjct: 216 NLFGLALTWFTQLEEGSIDNFKQLSTAFIKQYEYFINSDITEAHLWNFSQSADEPLRTYI 275
Query: 123 KRYSAASVKIEELEP*ACARA 143
R V E A RA
Sbjct: 276 YRVQGNHVNRPETIQDALHRA 296
>At2g12870 putative retroelement pol polyprotein
Length = 411
Score = 53.9 bits (128), Expect = 5e-08
Identities = 48/184 (26%), Positives = 82/184 (44%), Gaps = 16/184 (8%)
Query: 7 PFSAVVREVAIPDNMKSLALEAYSGGTDPKDHLLYFSMKMVISAASDAVK--------CR 58
PF+ + I D K L ++ ++G + PK HL F +I AA D K C
Sbjct: 61 PFTRKISSAIISDPGK-LRIDYFNGSSYPKGHLKSF----IIYAARDKFKPEERDAGLCH 115
Query: 59 MFPSMFKGTAMAWFTTLPLGSIVKFRDFSSKFLIQFSASKIKQVTID-DLYNVRQLERET 117
+F KG + F L + F + S+ FL QFS I T+D DL+++ Q E
Sbjct: 116 LFVEHLKGPVLVLFLRLEGNFVDSFEELSTLFLKQFSV-LIDPGTLDSDLWSLSQQPNEP 174
Query: 118 LKQYVKRYSAASVKIEELEP*ACARAFKDGL-LPGKLNNKLSRKLARSMTEVRAQANTYI 176
L + ++ + +E + A A K+ L + +L+ +++ + +A+ Y+
Sbjct: 175 LMDLLTKFRSTLASVEGIIDVAALSALKEALWYKSEFRKELNLSKPQTICDALHRASDYV 234
Query: 177 LDEE 180
EE
Sbjct: 235 SHEE 238
>At2g15410 putative retroelement pol polyprotein
Length = 1787
Score = 51.2 bits (121), Expect = 3e-07
Identities = 32/123 (26%), Positives = 59/123 (47%), Gaps = 5/123 (4%)
Query: 7 PFSAVVREVAIPDNMKSLALEAYSGGTDPKDHLLYFSMKMVISAASD----AVKCRMFPS 62
PF++ + +V I ++ + L Y G DP+ L S+ + + SD A C++F
Sbjct: 237 PFTSCISDVRIR-HISKIKLANYEGLVDPRPFLTSVSIAIGRAHFSDEDRDAGSCQLFVK 295
Query: 63 MFKGTAMAWFTTLPLGSIVKFRDFSSKFLIQFSASKIKQVTIDDLYNVRQLERETLKQYV 122
G A+ WF+ L SI ++ FL + K + DL+ + Q +E+L+ ++
Sbjct: 296 HLSGAALTWFSRLEANSIDSVHALTTSFLKNYGVFMEKGASNVDLWTMAQTAKESLRSFI 355
Query: 123 KRY 125
R+
Sbjct: 356 GRF 358
>At1g37060 Athila retroelment ORF 1, putative
Length = 1734
Score = 44.3 bits (103), Expect = 4e-05
Identities = 28/96 (29%), Positives = 42/96 (43%), Gaps = 4/96 (4%)
Query: 34 DPKDHLLYF----SMKMVISAASDAVKCRMFPSMFKGTAMAWFTTLPLGSIVKFRDFSSK 89
DP DHL F S+ + + D K R+FP A W +LP GSI + D
Sbjct: 61 DPLDHLDEFDRLCSLTKINRVSEDGFKLRLFPFSLGDKAHQWEKSLPQGSITSWNDCKKA 120
Query: 90 FLIQFSASKIKQVTIDDLYNVRQLERETLKQYVKRY 125
FL +F ++ +D+ Q ET + +R+
Sbjct: 121 FLAKFFSNSRTARLRNDISGFTQTNNETFYEAWERF 156
>At3g32290 hypothetical protein
Length = 612
Score = 43.5 bits (101), Expect = 6e-05
Identities = 40/175 (22%), Positives = 76/175 (42%), Gaps = 20/175 (11%)
Query: 7 PFSAVVREVAIPDNMKSLALEAYSGGTDPKDHLLYFSMKMVISAASDAVKCRMFPSMFKG 66
PF+ ++ I D K L +E ++G +DPK HL F +IS A + +
Sbjct: 164 PFTHMISNAIISDPGK-LRIEYFNGSSDPKGHLKSF----IISVARAKFRQEERDAG--- 215
Query: 67 TAMAWFTTLPLGSIVKFRDFSSKFLIQFSASKIKQVTIDDLYNVRQLERETLKQYVKRYS 126
S+ F++ S+ FL Q+S + DL+++ Q E L+ ++ ++
Sbjct: 216 -----------NSVDSFQELSTLFLKQYSVLIDLGTSDADLWSLSQQPNEPLRDFLAKFR 264
Query: 127 AASVKIEELEP*ACARAFKDGL-LPGKLNNKLSRKLARSMTEVRAQANTYILDEE 180
+ K+E + A A K L + +L+ ++ + +A+ Y+ EE
Sbjct: 265 STLAKVEGINDVAALSALKKALWYKSEFRKELNLSKPLTIRDALHRASDYVSHEE 319
>At1g41690 hypothetical protein
Length = 371
Score = 41.6 bits (96), Expect = 2e-04
Identities = 28/102 (27%), Positives = 43/102 (41%), Gaps = 4/102 (3%)
Query: 34 DPKDHLLYF----SMKMVISAASDAVKCRMFPSMFKGTAMAWFTTLPLGSIVKFRDFSSK 89
DP DHL F S+ + + D K R+FP A W TLP GSI + D
Sbjct: 61 DPLDHLDEFERLCSLTKINGVSEDGFKLRLFPFSLGDKAHLWEKTLPQGSITTWDDCKKA 120
Query: 90 FLIQFSASKIKQVTIDDLYNVRQLERETLKQYVKRYSAASVK 131
FL +F + +++ Q + E+ + +R+ K
Sbjct: 121 FLAKFFSYSRTARLRNEISGFTQKQSESFCEAWERFKGYQTK 162
>At2g07480 F9A16.15
Length = 1012
Score = 40.0 bits (92), Expect = 7e-04
Identities = 26/96 (27%), Positives = 43/96 (44%), Gaps = 4/96 (4%)
Query: 34 DPKDHLLYF----SMKMVISAASDAVKCRMFPSMFKGTAMAWFTTLPLGSIVKFRDFSSK 89
DP DHL F S+ + + ++ K R+FP A W TLP+ SI + D
Sbjct: 56 DPLDHLDNFDRLCSLTKINGVSEESFKLRLFPFSLGDKAHLWEKTLPVKSIDTWDDCKKA 115
Query: 90 FLIQFSASKIKQVTIDDLYNVRQLERETLKQYVKRY 125
FL +F ++ K ++ Q E+ + +R+
Sbjct: 116 FLAKFFSNSRKARLRSEISGFNQKNSESFSEAWERF 151
>At3g30655 hypothetical protein, 3' partial
Length = 660
Score = 39.7 bits (91), Expect = 9e-04
Identities = 26/102 (25%), Positives = 42/102 (40%), Gaps = 4/102 (3%)
Query: 34 DPKDHLLYFS----MKMVISAASDAVKCRMFPSMFKGTAMAWFTTLPLGSIVKFRDFSSK 89
DP DHL F + + + D K R+FP A W TLP SI + D
Sbjct: 61 DPLDHLDEFERLYGLTKINGVSEDGFKLRLFPFSLGDKAHLWEKTLPQNSITAWDDCKKA 120
Query: 90 FLIQFSASKIKQVTIDDLYNVRQLERETLKQYVKRYSAASVK 131
FL +F ++ +++ Q + E+ + +R+ K
Sbjct: 121 FLAKFFSNSRTARLRNEISGFTQKQNESFGEAWERFKGYQTK 162
>At4g20490 putative protein
Length = 853
Score = 38.1 bits (87), Expect = 0.003
Identities = 21/69 (30%), Positives = 33/69 (47%)
Query: 57 CRMFPSMFKGTAMAWFTTLPLGSIVKFRDFSSKFLIQFSASKIKQVTIDDLYNVRQLERE 116
C++F G A+ F+ L SI F S+ FL Q+ + DL+++ Q E
Sbjct: 222 CQLFVEGLTGNALTCFSRLEANSIDNFTQLSTAFLKQYRVFIQPGASSSDLWSMTQENGE 281
Query: 117 TLKQYVKRY 125
TL Y+ R+
Sbjct: 282 TLNDYLGRF 290
>At1g34080 putative protein
Length = 811
Score = 37.4 bits (85), Expect = 0.004
Identities = 35/142 (24%), Positives = 56/142 (38%), Gaps = 29/142 (20%)
Query: 7 PFSAVVREVAIPDNMKSLALEAYSGGTDPKDHLLYFSMKMVISAASDAVKCRMFPSMFKG 66
PF+ + ++ I + + L Y+G DPK++L F VI+
Sbjct: 193 PFTLRISKLRIRE-FQDFKLPGYNGKGDPKEYLTSFQ---VIAGRE-------------- 234
Query: 67 TAMAWFTTLPLGSIVKFRDFSSKFLIQFSASKIKQVTIDDLYNVRQLERETLKQYVKRYS 126
GSI F+ S+ F+ Q+ VT L+N+ Q E L+ Y +
Sbjct: 235 -----------GSIDNFKQLSTAFIKQYEYFIKSDVTEAQLWNLSQAADEPLRTYNTAFK 283
Query: 127 AASVKIEELEP*ACARAFKDGL 148
V++ L A A K+GL
Sbjct: 284 EIMVQVPGLSDSAAQSALKNGL 305
>At4g07640 putative athila transposon protein
Length = 866
Score = 35.8 bits (81), Expect = 0.013
Identities = 21/65 (32%), Positives = 30/65 (45%), Gaps = 4/65 (6%)
Query: 34 DPKDHLLYF----SMKMVISAASDAVKCRMFPSMFKGTAMAWFTTLPLGSIVKFRDFSSK 89
DP DHL F ++ + ++D K R+FP A W LP SI+ + D
Sbjct: 61 DPLDHLDEFDRLCNLTKINGVSADGFKLRLFPFSLGDKAHIWEKNLPHDSIITWDDCKKA 120
Query: 90 FLIQF 94
FL +F
Sbjct: 121 FLSKF 125
>At3g32890 Athila ORF 1, putative
Length = 755
Score = 35.8 bits (81), Expect = 0.013
Identities = 25/96 (26%), Positives = 41/96 (42%), Gaps = 4/96 (4%)
Query: 34 DPKDHLLYF----SMKMVISAASDAVKCRMFPSMFKGTAMAWFTTLPLGSIVKFRDFSSK 89
DP DHL F ++ + + D K R+FP A W LP SI + D
Sbjct: 14 DPLDHLDEFDRLYNLTKINGVSEDGFKLRLFPFSLGDKAHIWEKNLPHDSITTWDDCKKA 73
Query: 90 FLIQFSASKIKQVTIDDLYNVRQLERETLKQYVKRY 125
FL +F ++ +++ Q RE+ + +R+
Sbjct: 74 FLSKFFSNARTARLRNEISGFSQKTRESFCEAWERF 109
>At4g07780 putative athila transposon protein
Length = 446
Score = 35.0 bits (79), Expect = 0.022
Identities = 25/98 (25%), Positives = 40/98 (40%), Gaps = 4/98 (4%)
Query: 34 DPKDHLLYFS----MKMVISAASDAVKCRMFPSMFKGTAMAWFTTLPLGSIVKFRDFSSK 89
DP D+L F + + D K R+FP A W TLP SI + D
Sbjct: 69 DPLDNLDSFDRLCGLTKINGVTKDMFKLRLFPFSLGDKAHHWKKTLPPDSITSWDDCKKD 128
Query: 90 FLIQFSASKIKQVTIDDLYNVRQLERETLKQYVKRYSA 127
FL +F ++ +++ Q ET + +R+ +
Sbjct: 129 FLAKFFSNARTARLRNEISGFTQKNNETFFEASERFKS 166
>At2g13260 putative Athila retroelement ORF1 protein
Length = 622
Score = 35.0 bits (79), Expect = 0.022
Identities = 25/96 (26%), Positives = 40/96 (41%), Gaps = 4/96 (4%)
Query: 34 DPKDHLLYFSMKM----VISAASDAVKCRMFPSMFKGTAMAWFTTLPLGSIVKFRDFSSK 89
DP DHL F M + + DA K R+FP A W LP SI +
Sbjct: 79 DPLDHLEQFDMLCSTVKINGISEDAFKLRLFPFSLGDRARIWEKNLPQRSITSWDQCKRA 138
Query: 90 FLIQFSASKIKQVTIDDLYNVRQLERETLKQYVKRY 125
FL +F ++ +++ + Q E+ + +R+
Sbjct: 139 FLSKFFSTTRTARLRNEISSFTQRSNESFCEAWERF 174
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.323 0.135 0.381
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,421,129
Number of Sequences: 26719
Number of extensions: 119387
Number of successful extensions: 420
Number of sequences better than 10.0: 46
Number of HSP's better than 10.0 without gapping: 29
Number of HSP's successfully gapped in prelim test: 17
Number of HSP's that attempted gapping in prelim test: 377
Number of HSP's gapped (non-prelim): 51
length of query: 180
length of database: 11,318,596
effective HSP length: 93
effective length of query: 87
effective length of database: 8,833,729
effective search space: 768534423
effective search space used: 768534423
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0262.4