BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0253.3
(594 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g80410 putative N-terminal acetyltransferase (At1g80410) 653 0.0
At1g49870 hypothetical protein 37 0.024
At1g53110 proton pump interactor, putative 36 0.053
At5g36780 putative protein 36 0.069
At3g48710 unknown protein 35 0.090
At5g46020 unknown protein 35 0.15
At1g48760 putative protein 35 0.15
At1g29000 hypothetical protein 34 0.26
At5g53770 unknown protein 33 0.34
At4g13980 unknown protein 33 0.45
At3g02810 putative protein kinase 33 0.45
At5g03380 farnesylated protein - like 33 0.59
At1g15940 T24D18.4 33 0.59
At4g35940 putative protein 32 0.76
At3g57150 putative pseudouridine synthase (NAP57) 32 0.76
At3g04470 unknown protein 32 0.76
At2g19470 putative casein kinase I 32 0.76
At1g65200 hypothetical protein 32 0.76
At1g31870 unknown protein 32 0.76
At1g22530 unknown protein 32 0.76
>At1g80410 putative N-terminal acetyltransferase (At1g80410)
Length = 714
Score = 653 bits (1685), Expect = 0.0
Identities = 324/413 (78%), Positives = 372/413 (89%), Gaps = 3/413 (0%)
Query: 1 RIPLDFLQGDRFREAADSYMRPLLTKGVPSLFSDLSSLYDHPGKANILEQLILELENSIR 60
RIPLDFLQ + F+EA Y++PLLTKGVPSLFSDLSSLYDHP K +ILEQL++E+++SI
Sbjct: 303 RIPLDFLQDENFKEAVAKYIKPLLTKGVPSLFSDLSSLYDHPRKPDILEQLVVEMKHSIG 362
Query: 61 TTGTYPGRVEKEPPSTLMWILFLLSQHYDRRSQYEIALSKINEAIEHTPTVIDLYSVKSR 120
TTG++PG KEPPSTL+W LF L+QHYDRR QY++AL KI+EAI HTPTVIDLYSVKSR
Sbjct: 363 TTGSFPGSDVKEPPSTLLWTLFFLAQHYDRRGQYDVALCKIDEAIAHTPTVIDLYSVKSR 422
Query: 121 ILKHAGDLAAAAAFADEARCMDLSDRYVNSECVKRMLQADQVVLAEKTAVLFTKEGDQHN 180
I+KHAGDL AAAA ADEAR MDL+DRY+NSECVKRMLQADQV LAEKTAVLFTKEGDQ N
Sbjct: 423 IMKHAGDLTAAAALADEARGMDLADRYINSECVKRMLQADQVPLAEKTAVLFTKEGDQLN 482
Query: 181 NLHDMQCMWYELASAESYFRQGDLGLALKKFLAVEKHYTDITEDQFDFHSYCLRKMTLRT 240
NLHDMQCMWY+LAS +SYFRQGDLG ALKKFLAVEKHY DI+EDQFDFHSYCLRKMTLR+
Sbjct: 483 NLHDMQCMWYDLASGDSYFRQGDLGRALKKFLAVEKHYADISEDQFDFHSYCLRKMTLRS 542
Query: 241 YVEMLKFQDRLHSHVYFHKAAVGAIRCYIKLHDSPPKSTAEEDDDMSKLLPSQKKKLRQK 300
YV+MLKFQDRLHS YFHKAA+ AIRCY+KLHDS PKSTA E D+MSKL P+QKKK++ K
Sbjct: 543 YVDMLKFQDRLHSFPYFHKAAIRAIRCYLKLHDS-PKSTAGE-DEMSKLAPAQKKKIK-K 599
Query: 301 QRKAEARAKKEAEEKNEESSASGISKSGKRQAKPVDPDPRGEKLLQVEDPLLEATKYLKL 360
Q+KAEARAKKEAE K+EES+ASG SKSGKR KPVDPDP G+KL+QVE+P+ EA+KYL+L
Sbjct: 600 QKKAEARAKKEAESKSEESTASGASKSGKRNVKPVDPDPHGQKLIQVEEPMAEASKYLRL 659
Query: 361 LQKNSPDSLETHLLSFELHMRRQKILLAFQAVKQLLRLDAEHPDSHRCLIKFF 413
LQK+SP+SLETHLLSFE++MR+QK LLAFQAVKQLL+L AE+PDSHR L+KFF
Sbjct: 660 LQKHSPNSLETHLLSFEVNMRKQKFLLAFQAVKQLLKLGAENPDSHRSLVKFF 712
>At1g49870 hypothetical protein
Length = 828
Score = 37.4 bits (85), Expect = 0.024
Identities = 53/196 (27%), Positives = 81/196 (41%), Gaps = 42/196 (21%)
Query: 303 KAEARAKKEAEE--KNEESSASGISKSGKRQAKPVDPDPRGEKLLQVEDPLLEATKYLKL 360
K +R +KE EE KN S K+ R P+DP P E LL K++
Sbjct: 443 KHSSRLEKEIEEAKKNAGVSFGKYQKTSSRNKTPLDPIPDLESLL--------VKKHVSG 494
Query: 361 LQKNSPDSLETHLLSFELHMRRQKILLAFQAVKQLLRLD--AEHPDSHRCLIKFFHKVGS 418
L+K ++++ +E VK+ R D +E P CL+K F K
Sbjct: 495 LEKEVQETIKNCGKMYE-------------NVKKPGRKDGLSEVPSLDSCLVKHFSK--- 538
Query: 419 LNTPVNDSEKLVWSVVEAER-QTISQL-----QGKSLFETNKSFLEKYEDSLMHRAAFGE 472
L V +++K +EA +T+S + GK ++N + E E+SL
Sbjct: 539 LEKEVQEAKKRSKEDLEARNLETVSSVLLTEELGKENVDSNNNKAEGQEESL-------- 590
Query: 473 MMYVLDPSRRSEAVKI 488
M ++ P R E KI
Sbjct: 591 DMILVKPVHRLETEKI 606
>At1g53110 proton pump interactor, putative
Length = 439
Score = 36.2 bits (82), Expect = 0.053
Identities = 23/71 (32%), Positives = 35/71 (48%), Gaps = 1/71 (1%)
Query: 281 EEDDDMSKLLPSQKKKLRQKQR-KAEARAKKEAEEKNEESSASGISKSGKRQAKPVDPDP 339
EE + ++L +K+KL +K KA R KKEAE+K +E K ++ VD D
Sbjct: 302 EEQLEKARLAMERKRKLHEKAAAKAVIRVKKEAEKKRKELDKRAKKKKAVCKSSSVDVDR 361
Query: 340 RGEKLLQVEDP 350
E + + P
Sbjct: 362 TTETVSEASKP 372
>At5g36780 putative protein
Length = 565
Score = 35.8 bits (81), Expect = 0.069
Identities = 20/59 (33%), Positives = 33/59 (55%), Gaps = 1/59 (1%)
Query: 281 EEDDDMSKLLPSQKKKLRQKQR-KAEARAKKEAEEKNEESSASGISKSGKRQAKPVDPD 338
EE + ++L+ +K+KL++K KA RA+KEAE+K +E K+ P + D
Sbjct: 428 EEQLEKARLVMERKRKLQEKAAAKAAIRAQKEAEKKLKECEKKAKKKAAANSTSPSESD 486
>At3g48710 unknown protein
Length = 462
Score = 35.4 bits (80), Expect = 0.090
Identities = 17/57 (29%), Positives = 30/57 (51%)
Query: 277 KSTAEEDDDMSKLLPSQKKKLRQKQRKAEARAKKEAEEKNEESSASGISKSGKRQAK 333
K AEE+ + S +K RQ + + +KK+ +K++ S G +++ K QAK
Sbjct: 313 KPAAEEEKSIKGSAKSSRKSFRQVDKSTTSSSKKQKVDKDDSSKEKGKTQTSKPQAK 369
Score = 29.3 bits (64), Expect = 6.5
Identities = 18/56 (32%), Positives = 26/56 (46%), Gaps = 7/56 (12%)
Query: 281 EEDDDMSKLLPSQKKKLRQKQRKAEARAKKEAEEKNEESSASGISKSGKRQAKPVD 336
E+D K + KK+L + R KKE EE S G +KS ++ + VD
Sbjct: 289 EKDKAKEKTKSTDKKRLSK-------RTKKEKPAAEEEKSIKGSAKSSRKSFRQVD 337
>At5g46020 unknown protein
Length = 164
Score = 34.7 bits (78), Expect = 0.15
Identities = 33/110 (30%), Positives = 58/110 (52%), Gaps = 7/110 (6%)
Query: 292 SQKKKLRQKQRKAEARAKKEAEEKNEESS--ASGISKSGKRQAKPVD-PDPRGEKLLQVE 348
++ + +QK+ + E ++E+EE++EE S + + K G VD P+ +K L+ +
Sbjct: 29 ARPRSFKQKEAEYEEDVEEESEEESEEESEDEADVKKKGAEAVIEVDNPNRVRQKTLKAK 88
Query: 349 DPLLEATKYLKLLQKNSPDSLETHLLSFELHMRRQKILLAFQAVKQLLRL 398
D L+A+K + L + + LE + E +MR Q+ QA K L RL
Sbjct: 89 D--LDASKTTE-LSRREREELEKQ-RAHERYMRLQEQGKTEQARKDLDRL 134
>At1g48760 putative protein
Length = 869
Score = 34.7 bits (78), Expect = 0.15
Identities = 17/54 (31%), Positives = 34/54 (62%), Gaps = 3/54 (5%)
Query: 272 HDSPPKSTAEEDDDMSKL---LPSQKKKLRQKQRKAEARAKKEAEEKNEESSAS 322
++ P S +E ++ S++ S+KKK ++K++K E +K ++ +NE +SAS
Sbjct: 806 YEGNPNSGQQEKEESSRIENHQNSEKKKKKKKKKKGEGSSKHKSRRQNEVASAS 859
>At1g29000 hypothetical protein
Length = 287
Score = 33.9 bits (76), Expect = 0.26
Identities = 20/78 (25%), Positives = 40/78 (50%), Gaps = 3/78 (3%)
Query: 270 KLHDSPPKSTAEEDDDMSKLLPSQKKKLRQKQRKAEARAKKEAEEKNEESSASGISKSGK 329
K+H +++ +++ K +KKK + ++K E KKE E+K EE + K G+
Sbjct: 170 KVHKHAEIISSKTEEEKKKEEEDKKKKEEEDKKKKEDEKKKEEEKKKEEENK---KKEGE 226
Query: 330 RQAKPVDPDPRGEKLLQV 347
++ + V + + + QV
Sbjct: 227 KKKEEVKVEVTTKTITQV 244
>At5g53770 unknown protein
Length = 530
Score = 33.5 bits (75), Expect = 0.34
Identities = 18/71 (25%), Positives = 37/71 (51%), Gaps = 2/71 (2%)
Query: 263 GAIRCYIKLHDSPPKSTAEEDDDMSKLLPSQKKKLRQKQRKAEARAKK--EAEEKNEESS 320
G + C +L + + + +D++ ++ + KK ++ RK + ++KK E +E +EE
Sbjct: 450 GGLFCNWELEEEEEEGSFPRGNDITPVVDTPGKKSKESSRKKKKKSKKNKEVDEDDEEEG 509
Query: 321 ASGISKSGKRQ 331
S K KR+
Sbjct: 510 TSTKKKKKKRR 520
>At4g13980 unknown protein
Length = 466
Score = 33.1 bits (74), Expect = 0.45
Identities = 37/168 (22%), Positives = 67/168 (39%), Gaps = 21/168 (12%)
Query: 269 IKLHDSPPKSTAEEDDDMSKLLPSQKKKLRQKQRKAEARAKKEAEEK-----NEESSASG 323
I H PP S+ +++ +L Q KL +++ EA+ K ++K E
Sbjct: 116 IHSHSHPPASSTDQE---RAVLQEQMDKLSREKAAIEAKLLKFKQQKVVAKHQFEEMTEH 172
Query: 324 ISKSGKRQAK-----------PVDPDPRGEKLLQVEDPLLEATKYLKLLQKNSPDSLETH 372
+ RQ K P G+K+ Q++ + L ++++ P S ++H
Sbjct: 173 VDDMENRQKKLLNFLETAIRNPTFVKNFGKKVEQLDISAYNKKRRLPEVEQSKPPSEDSH 232
Query: 373 LLSFELHMRRQKILLAFQAVKQLLRLDAEHPDSHRCLIKFFHKVGSLN 420
L + RR+ + Q LRL+ DS ++ H + S N
Sbjct: 233 LDNSSGSSRRESGNIFHQNFSNKLRLELSPADSDMNMVS--HSIQSSN 278
>At3g02810 putative protein kinase
Length = 558
Score = 33.1 bits (74), Expect = 0.45
Identities = 20/70 (28%), Positives = 29/70 (40%)
Query: 274 SPPKSTAEEDDDMSKLLPSQKKKLRQKQRKAEARAKKEAEEKNEESSASGISKSGKRQAK 333
SPP A EDD S + +K+ ++ KK+ EE++ + S S K
Sbjct: 378 SPPPELATEDDKSSTSSGEESSLESEKESVSKNEYKKKHEEEDSSMESDDESDSNSEHEK 437
Query: 334 PVDPDPRGEK 343
P P EK
Sbjct: 438 DQPPKPIDEK 447
>At5g03380 farnesylated protein - like
Length = 392
Score = 32.7 bits (73), Expect = 0.59
Identities = 30/140 (21%), Positives = 63/140 (44%), Gaps = 17/140 (12%)
Query: 209 KKFLAVEKHYTDITEDQFDFHSYCLRKMTLRTYVEMLKFQDRLHSHVYFHKAAVGAIRCY 268
KK + KH+ + + + D+ S K+T+ V+ ++ +D++ + V +
Sbjct: 39 KKIKRIFKHFKGVEDVKIDYKS---NKLTVIGNVDPVEVRDKVADKIKRPVELVSTVAPP 95
Query: 269 IKLHDSPPKSTAEEDDDMSKLLPSQKKKLRQKQRKAEARAKKEAEEKNEESSASGISKSG 328
K ++PP S E K P+ ++K +K+ A + ++ EEK +E G
Sbjct: 96 KK--ETPPSSGGAE----KKPSPAAEEKPAEKKPAAVEKPGEKKEEKKKEE--------G 141
Query: 329 KRQAKPVDPDPRGEKLLQVE 348
+++A P P +L+ +
Sbjct: 142 EKKASPPPPPKESTVVLKTK 161
>At1g15940 T24D18.4
Length = 1012
Score = 32.7 bits (73), Expect = 0.59
Identities = 23/109 (21%), Positives = 50/109 (45%), Gaps = 2/109 (1%)
Query: 269 IKLHDSPPKSTAEEDDDMSKLLPSQKKKLRQKQRKAEARAKKEAEEKNEESSASGISKSG 328
+K ++ PKS EE + + K ++ E A+++ +E+ E A + +
Sbjct: 849 LKEPNAEPKSDGEEQEAAKEPNAELKTDGENQEAAKELTAERKTDEE-EHKVADEVEQKS 907
Query: 329 KRQAKPVDPDPRGEKLLQVEDPLLEATKYLKLLQKNSPDSLETHLLSFE 377
+++ V+P+ GE+ VE+P E ++ + + +T L+ E
Sbjct: 908 QKETN-VEPEAEGEEQKSVEEPNAEPKTKVEEKESAKEQTADTKLIEKE 955
>At4g35940 putative protein
Length = 451
Score = 32.3 bits (72), Expect = 0.76
Identities = 17/56 (30%), Positives = 31/56 (55%), Gaps = 1/56 (1%)
Query: 294 KKKLRQKQRKAEARAKKEAEEKNEESSASGISKSGKRQAKPVDPDPRGEKLLQVED 349
KK+ R+K R+++ + KK+ +E+ E+ K +R+ K V + R K + ED
Sbjct: 35 KKEQRRKDRRSDKKDKKDKKERKEKKEKKE-KKRKEREGKEVGSEKRSHKRRRKED 89
Score = 29.3 bits (64), Expect = 6.5
Identities = 28/98 (28%), Positives = 47/98 (47%), Gaps = 6/98 (6%)
Query: 293 QKKKLRQKQRKAEARAKKEAEEKNEESSASGISKSGKRQAKPVDPDPRG--EKLLQVEDP 350
+K K +K+RK E + KKE + K E G K ++ + D KL + E
Sbjct: 47 KKDKKDKKERK-EKKEKKEKKRKEREGKEVGSEKRSHKRRRKEDGAKVDLFHKLKESEVN 105
Query: 351 LLE---ATKYLKLLQKNSPDSLETHLLSFELHMRRQKI 385
LE T +LLQ S +S ++ L S E+ +++++
Sbjct: 106 CLEKSSLTVERELLQSTSQNSCDSTLNSNEMLPKQKEV 143
>At3g57150 putative pseudouridine synthase (NAP57)
Length = 565
Score = 32.3 bits (72), Expect = 0.76
Identities = 24/88 (27%), Positives = 40/88 (45%), Gaps = 6/88 (6%)
Query: 272 HDSPPKSTAEEDDDMSKLLPSQKKKLRQKQRKAEARAKKEAEEKNEESSASGISKSGKRQ 331
HD S A SK + ++ +K + ++ + KK+ EE+ EE +G K K++
Sbjct: 444 HDDSSDSPAPVTTKKSKTKEVEGEEAEEKVKSSKKKKKKDKEEEKEEE--AGSEKKEKKK 501
Query: 332 AKPVDPDPRGEKLLQVEDPLLEATKYLK 359
K D + E + +V P E K K
Sbjct: 502 KK----DKKEEVIEEVASPKSEKKKKKK 525
>At3g04470 unknown protein
Length = 640
Score = 32.3 bits (72), Expect = 0.76
Identities = 19/43 (44%), Positives = 26/43 (60%), Gaps = 5/43 (11%)
Query: 288 KLLPSQKKKLRQKQRKAEARAKKEAEEKNEESSASGISKSGKR 330
K + S +KK R K +KA++R KK+A K A+G S SG R
Sbjct: 596 KWIDSAEKKRRMKAKKAKSRRKKQAATK-----AAGASDSGTR 633
>At2g19470 putative casein kinase I
Length = 433
Score = 32.3 bits (72), Expect = 0.76
Identities = 20/68 (29%), Positives = 32/68 (46%), Gaps = 9/68 (13%)
Query: 201 QGDLGLALKKFLAVEKHYTDITEDQF-----DFHSYCLRKMTLRTYV----EMLKFQDRL 251
QG G+ K+ VE Y + D D SYC R+ +L+T + +M+ + +
Sbjct: 60 QGGTGIPNMKWYGVEGDYNVLVMDLLGPSLEDLFSYCKRQFSLKTVLMLADQMINRLEFI 119
Query: 252 HSHVYFHK 259
HS Y H+
Sbjct: 120 HSKSYLHR 127
>At1g65200 hypothetical protein
Length = 1101
Score = 32.3 bits (72), Expect = 0.76
Identities = 43/173 (24%), Positives = 70/173 (39%), Gaps = 27/173 (15%)
Query: 210 KFLAVEKHYTDITEDQ-----FDFHSYCLRKMTLRTYVEMLKFQDRLHSHVYFHKAAVGA 264
+FL VE+ ++ FD+ SY LR M E++ L VY A
Sbjct: 645 RFLLVERSRINLLNSLTRLSVFDYRSYILRPMK-----ELI-----LDGIVYMENKA--- 691
Query: 265 IRCYIKLHDSPPKSTAEEDDDMSKLLPSQKKKLRQKQRKAEARAKKEAEEKNEESSASGI 324
KL +EE + LPS+KKK + ++ + + + + E SA+
Sbjct: 692 -----KLAVVEADLISEEKQEEGMTLPSKKKKNKNNKKNSTSMSSHLDKTVQHEHSANLE 746
Query: 325 SKSGKRQAKPVDPDPRGEK--LLQVEDPLLEATKYLKLLQKNSPDSLETHLLS 375
S KPV+ D + LL+++ + + K L + DSL H +S
Sbjct: 747 LDSTSPSLKPVEEDTLTSESGLLEMKSNINNQEETTKAL--HGEDSLSKHPVS 797
>At1g31870 unknown protein
Length = 561
Score = 32.3 bits (72), Expect = 0.76
Identities = 23/82 (28%), Positives = 40/82 (48%), Gaps = 1/82 (1%)
Query: 310 KEAEEKNEESSASGISKSGKRQAKPVDPDPRGEKLLQVEDPLLEATKYLKLLQKNSPDSL 369
K+ +K E S K K+Q KP P+PRG L+ EDP+ + + + +
Sbjct: 8 KDYLKKYESSDVVEKKKKKKKQKKPSKPEPRG-VLVVDEDPVWQKQVDPEEDENEDDSAE 66
Query: 370 ETHLLSFELHMRRQKILLAFQA 391
ET L+ ++ ++R + L +A
Sbjct: 67 ETPLVDEDIEVKRMRRLEEIKA 88
>At1g22530 unknown protein
Length = 683
Score = 32.3 bits (72), Expect = 0.76
Identities = 20/82 (24%), Positives = 35/82 (42%), Gaps = 15/82 (18%)
Query: 275 PPKSTAEEDDDMSKLLPSQKKKLRQKQRKAEARAKKEAEEKNEESSASGISKSGKRQAKP 334
PP EE + +++K E +K+ E K EE S +K ++ A P
Sbjct: 120 PPAPVKEE---------------KVEEKKTEETEEKKEEVKTEEKSLEAETKEEEKSAAP 164
Query: 335 VDPDPRGEKLLQVEDPLLEATK 356
+ + E++L P++ TK
Sbjct: 165 ATVETKKEEILAAPAPIVAETK 186
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.317 0.132 0.378
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,411,418
Number of Sequences: 26719
Number of extensions: 578494
Number of successful extensions: 3394
Number of sequences better than 10.0: 126
Number of HSP's better than 10.0 without gapping: 32
Number of HSP's successfully gapped in prelim test: 96
Number of HSP's that attempted gapping in prelim test: 3101
Number of HSP's gapped (non-prelim): 284
length of query: 594
length of database: 11,318,596
effective HSP length: 105
effective length of query: 489
effective length of database: 8,513,101
effective search space: 4162906389
effective search space used: 4162906389
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 63 (28.9 bits)
Lotus: description of TM0253.3