BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0197.5
(317 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g00610 hypothetical protein 31 0.77
At1g58060 hypothetical protein 31 0.77
At1g04180 putative dimethylaniline monooxygenase 31 0.77
At5g55300 DNA topoisomerase I 31 1.0
At3g55580 regulator of chromosome condensation-like protein 31 1.0
At3g57150 putative pseudouridine synthase (NAP57) 30 1.7
At2g41310 putative two-component response regulator 3 protein 30 1.7
At1g18410 kinesin-related protein, putative 30 1.7
At1g73860 kinesin-related protein 30 2.3
At1g24190 hypothetical protein 29 3.8
At5g38880 putative protein 28 5.0
At2g43690 putative receptor protein kinase 28 5.0
At2g39800 delta-1-pyrroline 5-carboxylase synthetase (P5C1) 28 5.0
At4g28720 unknown protein 28 6.5
At3g54760 Putative protein 28 6.5
At2g14910 unknown protein 28 6.5
At2g07440 putative two-component response regulator protein 28 6.5
At2g05290 putative Ta11-like non-LTR retroelement protein 28 6.5
At4g08750 putative protein 28 8.6
At3g49600 putative protein 28 8.6
>At4g00610 hypothetical protein
Length = 328
Score = 31.2 bits (69), Expect = 0.77
Identities = 21/70 (30%), Positives = 29/70 (41%), Gaps = 1/70 (1%)
Query: 73 SNGVSTARKP-ETVVGESSSERDQYRPESDWNRSSVSRKANIAREVSSPVAVEQVAGSKP 131
S S +KP E V SS E ++ P SD S S A ++ A + K
Sbjct: 66 SGVTSPVKKPLEAVASTSSEEEEEDEPSSDSESGSESESDTEAEPMTLAAAAPSSSNEKR 125
Query: 132 QAENANHEEK 141
Q+E EE+
Sbjct: 126 QSEGKPEEER 135
>At1g58060 hypothetical protein
Length = 1389
Score = 31.2 bits (69), Expect = 0.77
Identities = 33/124 (26%), Positives = 54/124 (42%), Gaps = 16/124 (12%)
Query: 23 RVKRLGAI--KVLLEFEIGEDMRFMLAEL----TIEAMIDGWLFIVLLEMEGLVVFSNGV 76
+ K+L + K+ E + + + L+ L T EA +D WL + L E V FS G
Sbjct: 71 KTKKLNNVYEKLSCEGFVDDQIELALSSLRDGATFEAALD-WLCLNLPSHELPVKFSTGA 129
Query: 77 STARKPETVVGESSSERDQYRPESDWNRSSVSRKANIAREVSSPVAVEQVAGSKPQAENA 136
S VG S+ RD DWN S+ S ++ E P +V G + + +
Sbjct: 130 SRFPSTGGSVGVISTSRD------DWNDSTDS---SVRVEEEEPAVFVRVKGKQDEEDTL 180
Query: 137 NHEE 140
+ ++
Sbjct: 181 SSDK 184
>At1g04180 putative dimethylaniline monooxygenase
Length = 421
Score = 31.2 bits (69), Expect = 0.77
Identities = 27/124 (21%), Positives = 54/124 (42%), Gaps = 8/124 (6%)
Query: 166 VTAEARHVVKNADVGLMRKEVTVLMVGWVHNWLVGSLRMGTRWVV--QINNVWLRSEQVC 223
V + HV+ +G ++V+M+ W+ WLV L + W+V ++N L+ +
Sbjct: 218 VVRSSVHVLPREIMGKSTFGISVMMMKWLPLWLVDKLLLILSWLVLGSLSNYGLKRPDIG 277
Query: 224 GDTQKAAKEDSWGASDASLQQVMVGEHE----VKDFSPDQARV--SQVVDARNTNIQDGF 277
K+ + +L+++ G+ E +K FS + Q +D + G+
Sbjct: 278 PMELKSMTGKTPVLDIGALEKIKSGDVEIVPAIKQFSRHHVELVDGQKLDIDAVVLATGY 337
Query: 278 RSGI 281
RS +
Sbjct: 338 RSNV 341
>At5g55300 DNA topoisomerase I
Length = 916
Score = 30.8 bits (68), Expect = 1.0
Identities = 25/80 (31%), Positives = 37/80 (46%), Gaps = 5/80 (6%)
Query: 82 PETVVGESSSERDQYRPESDWNRSSVSRK-ANIAREVSSPVAVEQVAGSKPQAENANHEE 140
P TV S ++DQ + + S R ++I P + QV S PQ E N+ +
Sbjct: 99 PSTVKDRSQLQKDQSECKIEHEDSEDDRPLSSILSGNKGPTSSRQV--SSPQPEKKNNGD 156
Query: 141 KPLDLGRMSHLVCGESKGAT 160
+PLD R S ++ ES T
Sbjct: 157 RPLD--RASRIIKDESDDET 174
>At3g55580 regulator of chromosome condensation-like protein
Length = 488
Score = 30.8 bits (68), Expect = 1.0
Identities = 37/143 (25%), Positives = 60/143 (41%), Gaps = 15/143 (10%)
Query: 48 ELTIEAMIDGWLFIVLLEMEGLVVFSNG----VSTARKPETVVGES-----SSERDQYRP 98
E+ ++ GW V + E V++ G + T R V G+S S +Q
Sbjct: 116 EVCVQKAEAGWAHCVAVT-ENQQVYTWGWRECIPTGRVFGQVDGDSCERNISFSTEQVSS 174
Query: 99 ESDWNRSSVSRKANI-AREVSSPVAVEQVAGSKPQAENANHEEKPLDLGRMSHLVCGESK 157
S +SS + + R P +++ SK AEN++ + +DL S L C S
Sbjct: 175 SSQGKKSSGGTSSQVEGRGGGEPTKKRRISPSKQAAENSSQSDN-IDL---SALPCLVSL 230
Query: 158 GATEEAMSVTAEARHVVKNADVG 180
+SV A RH + +D+G
Sbjct: 231 APGVRIVSVAAGGRHTLALSDIG 253
>At3g57150 putative pseudouridine synthase (NAP57)
Length = 565
Score = 30.0 bits (66), Expect = 1.7
Identities = 22/91 (24%), Positives = 39/91 (42%), Gaps = 2/91 (2%)
Query: 58 WLFIVLLEMEGLVVFSNGVSTAR--KPETVVGESSSERDQYRPESDWNRSSVSRKANIAR 115
W V+L G + + + K + GE+ R + +S + + V+ K + +
Sbjct: 403 WSRNVVLPTGGDAIIAGAAAAPEEIKADAENGEAGEARKRKHDDSSDSPAPVTTKKSKTK 462
Query: 116 EVSSPVAVEQVAGSKPQAENANHEEKPLDLG 146
EV A E+V SK + + EEK + G
Sbjct: 463 EVEGEEAEEKVKSSKKKKKKDKEEEKEEEAG 493
>At2g41310 putative two-component response regulator 3 protein
Length = 225
Score = 30.0 bits (66), Expect = 1.7
Identities = 11/36 (30%), Positives = 20/36 (55%)
Query: 110 KANIAREVSSPVAVEQVAGSKPQAENANHEEKPLDL 145
K + +E PVA+E++ SKP+ E E +++
Sbjct: 146 KTKLKKESEKPVAIEEIVVSKPEIEEEEEESSVIEI 181
>At1g18410 kinesin-related protein, putative
Length = 1081
Score = 30.0 bits (66), Expect = 1.7
Identities = 19/59 (32%), Positives = 32/59 (54%), Gaps = 4/59 (6%)
Query: 63 LLEMEGLVVFSNGVSTARKPETVVGESSSERDQ----YRPESDWNRSSVSRKANIAREV 117
+L+ + +F+ G + + K T+ G + ++ YR +D R S SRK+NIA EV
Sbjct: 704 VLDGYNVCIFAYGQTGSGKTYTMTGPDGASEEEWGVNYRALNDLFRISQSRKSNIAYEV 762
>At1g73860 kinesin-related protein
Length = 1050
Score = 29.6 bits (65), Expect = 2.3
Identities = 21/59 (35%), Positives = 33/59 (55%), Gaps = 4/59 (6%)
Query: 63 LLEMEGLVVFSNGVSTARKPETVVG-ESSSERD---QYRPESDWNRSSVSRKANIAREV 117
+L+ + +F+ G + + K T+ G + SSE D YR +D + S SRK NI+ EV
Sbjct: 601 VLDGYNVCIFAYGQTGSGKTYTMTGPDGSSEEDWGVNYRALNDLFKISQSRKGNISYEV 659
>At1g24190 hypothetical protein
Length = 1263
Score = 28.9 bits (63), Expect = 3.8
Identities = 24/84 (28%), Positives = 38/84 (44%), Gaps = 10/84 (11%)
Query: 87 GESSSERDQYRPESDWNRSSVSRKANIAREVSSPVAVEQ--VAGSKPQA--ENANHEEKP 142
GES Y +D RS SRKAN ++ E+ AG A E A HE+
Sbjct: 738 GESEGSPHNYASVADSRRSKSSRKANEHSQLGQTSNSERDGAAGRTSDALCETAQHEK-- 795
Query: 143 LDLGRMSHLVCGESKGATEEAMSV 166
+ ++V + K +++A+S+
Sbjct: 796 ----MLKNVVTSDEKPESKQAVSI 815
>At5g38880 putative protein
Length = 775
Score = 28.5 bits (62), Expect = 5.0
Identities = 19/58 (32%), Positives = 27/58 (45%), Gaps = 3/58 (5%)
Query: 80 RKPETVVGESSS---ERDQYRPESDWNRSSVSRKANIAREVSSPVAVEQVAGSKPQAE 134
RK +TV GESSS +R+ E + V R NI R + + S+ +AE
Sbjct: 83 RKDKTVTGESSSYAEDREAALQERELAAKEVERLRNIVRRQRKDLKARMLEVSREEAE 140
>At2g43690 putative receptor protein kinase
Length = 664
Score = 28.5 bits (62), Expect = 5.0
Identities = 14/62 (22%), Positives = 31/62 (49%), Gaps = 3/62 (4%)
Query: 206 TRWVVQINNVWLRSEQVCGDTQKAAKEDSWGASDASLQQVMVGEHEVKDFSPDQARVSQV 265
T W + N W + V T++ +++ G + L+ ++ HE ++ PD A V ++
Sbjct: 549 TNWAI---NCWENGDIVEAATERIRQDNDKGQLELVLKLGVLCSHEAEEVRPDMATVVKI 605
Query: 266 VD 267
++
Sbjct: 606 LN 607
>At2g39800 delta-1-pyrroline 5-carboxylase synthetase (P5C1)
Length = 717
Score = 28.5 bits (62), Expect = 5.0
Identities = 19/64 (29%), Positives = 28/64 (43%), Gaps = 7/64 (10%)
Query: 232 EDSWGASD-------ASLQQVMVGEHEVKDFSPDQARVSQVVDARNTNIQDGFRSGIAVD 284
ED +GA D A ++ +HEV + Q + V +T DGFR G+ +
Sbjct: 608 EDVYGAIDHIHRHGSAHTDCIVTEDHEVAELFLRQVDSAAVFHNASTRFSDGFRFGLGAE 667
Query: 285 DDVS 288
VS
Sbjct: 668 VGVS 671
>At4g28720 unknown protein
Length = 426
Score = 28.1 bits (61), Expect = 6.5
Identities = 24/124 (19%), Positives = 55/124 (44%), Gaps = 8/124 (6%)
Query: 166 VTAEARHVVKNADVGLMRKEVTVLMVGWVHNWLVGSLRMGTRWVV--QINNVWLRSEQVC 223
V + HV+ +G E+ + M+ W WLV + + W+V I L+ ++
Sbjct: 221 VVRSSLHVMPREVMGKSTFELAMKMLRWFPLWLVDKILLVLSWMVLGNIEKYGLKRPEMG 280
Query: 224 GDTQKAAKEDSWGASDASLQQVMVGEHEV----KDFSPDQARV--SQVVDARNTNIQDGF 277
K+ K + +++++ +G+ V K F+ ++ + + +D + + G+
Sbjct: 281 PMELKSVKGKTPVLDIGAIEKIRLGKINVVPGIKRFNGNKVELVNGEQLDVDSVVLATGY 340
Query: 278 RSGI 281
RS +
Sbjct: 341 RSNV 344
>At3g54760 Putative protein
Length = 792
Score = 28.1 bits (61), Expect = 6.5
Identities = 23/93 (24%), Positives = 38/93 (40%), Gaps = 11/93 (11%)
Query: 78 TARKPETVVGESSSERDQYRPESDWNRSSVSRKANIAREVSSPVAVEQVAGSKPQAENA- 136
T +K E + E + Q E++ + S A + E S+ ++ E+ + EN
Sbjct: 171 TTKKIEKDINEMEVDSKQEDEENETEDAKHSESAQVPEE-STKLSKEETDEENQKEENGV 229
Query: 137 ---------NHEEKPLDLGRMSHLVCGESKGAT 160
H EK + +G S C ES GA+
Sbjct: 230 AMEIDGVAGEHVEKVVKVGSGSDNACNESAGAS 262
>At2g14910 unknown protein
Length = 386
Score = 28.1 bits (61), Expect = 6.5
Identities = 28/119 (23%), Positives = 54/119 (44%), Gaps = 19/119 (15%)
Query: 174 VKNADVGLMRKEVTVLMVGWVHNWLVGSLRM--GTRWVVQINNVW------LRSEQVCGD 225
++ DV L++K+V V + + + G L + R+ V I ++W L S + G
Sbjct: 88 IEPLDVSLIQKDVPVTTLDAMKRTISGMLGLLPSDRFQVHIESLWEPLSKLLVSSMMTGY 147
Query: 226 TQKAAK---------EDSWGASDASLQQVMVGEHEVKDFSPDQARVSQVVDARNTNIQD 275
T + A+ + S G D+ + E++++ PD+ VS D+R N+ +
Sbjct: 148 TLRNAEYRLFLEKNLDMSGGGLDSHASENT--EYDMEGTFPDEDHVSSKRDSRTQNLSE 204
>At2g07440 putative two-component response regulator protein
Length = 136
Score = 28.1 bits (61), Expect = 6.5
Identities = 16/64 (25%), Positives = 32/64 (50%), Gaps = 10/64 (15%)
Query: 110 KANIAREVSSPVAVEQVAGSKPQAENAN--------HEEKPLDLGRMSHLVCGESKGATE 161
K + +E PVA+E++ SKP+ E + H+E ++ ++ ++ + A E
Sbjct: 60 KTKLKKESEKPVAIEEIVVSKPEIEEESLVIDILPLHQE--VESEQLEPMLSSNKRKAME 117
Query: 162 EAMS 165
E +S
Sbjct: 118 EVIS 121
>At2g05290 putative Ta11-like non-LTR retroelement protein
Length = 383
Score = 28.1 bits (61), Expect = 6.5
Identities = 15/59 (25%), Positives = 29/59 (48%), Gaps = 8/59 (13%)
Query: 64 LEMEGLVVFSNGVSTARKPETVVGESSSERDQYRP--------ESDWNRSSVSRKANIA 114
LE + F+N ST ++P + + + + + R +SD NR+S+ ++ IA
Sbjct: 306 LEASSSISFTNDTSTCKRPRSWIRNTKDRKGKNRKIMSESLENKSDLNRNSLEKRKGIA 364
>At4g08750 putative protein
Length = 461
Score = 27.7 bits (60), Expect = 8.6
Identities = 23/77 (29%), Positives = 37/77 (47%), Gaps = 4/77 (5%)
Query: 73 SNGVSTARKPETVVGESSSERDQY--RPESDWNRSSVSRKANI--AREVSSPVAVEQVAG 128
S+ VS P V + +++ QY S S VS N+ A + S A +
Sbjct: 244 SSPVSYHSPPARVSADRTTQLGQYLISSPSSVELSGVSETKNLDAADKNGSLTAKAESIA 303
Query: 129 SKPQAENANHEEKPLDL 145
SK ++++ +H+EK LDL
Sbjct: 304 SKDESKDNSHDEKKLDL 320
>At3g49600 putative protein
Length = 1672
Score = 27.7 bits (60), Expect = 8.6
Identities = 15/54 (27%), Positives = 27/54 (49%)
Query: 249 EHEVKDFSPDQARVSQVVDARNTNIQDGFRSGIAVDDDVSQVCVTPQEGSKRRR 302
EH D + +V + VD ++ +++ + DDDV V ++ SK+RR
Sbjct: 169 EHSFLDRDSGRKKVDEDVDEKDAKVKESKKQRGGDDDDVDVVKRHKKKESKKRR 222
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.316 0.131 0.375
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,549,466
Number of Sequences: 26719
Number of extensions: 254714
Number of successful extensions: 749
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 15
Number of HSP's that attempted gapping in prelim test: 740
Number of HSP's gapped (non-prelim): 23
length of query: 317
length of database: 11,318,596
effective HSP length: 99
effective length of query: 218
effective length of database: 8,673,415
effective search space: 1890804470
effective search space used: 1890804470
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0197.5