BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0185.2
(122 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g62520 putative protein 110 1e-25
At2g14950 Ac-like transposase 92 6e-20
At4g12260 putative transposase 91 1e-19
At1g42110 hypothetical protein 90 3e-19
At3g45270 putative protein 89 4e-19
At1g80020 hypothetical protein 87 2e-18
At5g39060 transposase -like protein 86 4e-18
At1g59770 hypothetical protein 86 6e-18
At3g29120 hypothetical protein 85 1e-17
At1g15300 unknown protein 84 1e-17
At1g49550 hypothetical protein 84 2e-17
At3g42170 putative transposase 83 4e-17
At4g05510 81 1e-16
At1g69950 unknown protein 81 1e-16
At3g14800 unknown protein 77 2e-15
At2g18580 Ac-like transposase 77 2e-15
At5g39070 putative protein 76 5e-15
At1g18560 hypothetical protein 72 9e-14
At4g13120 putative protein (possibly fragment) 70 2e-13
At4g07540 putative transposon protein 70 2e-13
>At3g62520 putative protein
Length = 705
Score = 110 bits (276), Expect = 1e-25
Identities = 56/119 (47%), Positives = 81/119 (68%), Gaps = 11/119 (9%)
Query: 4 NELTKYLEDGLE--ERGSL----DILNWWKLNASRYPILASIARELLAIPISTVASESTF 57
NEL KYL+D L SL D+L+WWK N+S+YPI++ +ARE+LAIP+S+VASES F
Sbjct: 574 NELVKYLKDELHVTTENSLGLPFDLLDWWKTNSSKYPIMSLMAREVLAIPVSSVASESAF 633
Query: 58 SAGGRVVDPYRSSLTPKTLEALICTQDWIKGKYSKSLLSNEEVME-----LEKYEQGII 111
S GGR++D YRS LTP +EAL+ TQDW++ + + + +E ++ E+GI+
Sbjct: 634 STGGRILDQYRSCLTPDMVEALVLTQDWLRASLRSEAMKSLDKLEEENKFMDSLEEGIL 692
>At2g14950 Ac-like transposase
Length = 730
Score = 92.0 bits (227), Expect = 6e-20
Identities = 43/95 (45%), Positives = 65/95 (68%), Gaps = 5/95 (5%)
Query: 2 SKNELTKYLEDGLEERGSL-----DILNWWKLNASRYPILASIARELLAIPISTVASEST 56
S NEL YL + +E L D+L+WWKLN+ ++ +L+ IA+++ A+ +S+VASES
Sbjct: 603 SSNELDLYLNEAVETPHLLMGIESDVLDWWKLNSGKFLVLSLIAKDIFAMQVSSVASESA 662
Query: 57 FSAGGRVVDPYRSSLTPKTLEALICTQDWIKGKYS 91
FS GRV+DP+RS LT +E L+CT+ W+K + S
Sbjct: 663 FSTSGRVLDPFRSCLTHYMIEVLMCTEQWLKSEIS 697
>At4g12260 putative transposase
Length = 220
Score = 91.3 bits (225), Expect = 1e-19
Identities = 48/119 (40%), Positives = 77/119 (64%), Gaps = 12/119 (10%)
Query: 3 KNELTKYLEDGLEERGSL-----DILNWWKLNASRYPILASIARELLAIPISTVASESTF 57
K+EL YL+ +E +L D+L+WW+LN+ +YP+L+ IAR++LA+ +S VASES F
Sbjct: 95 KDELDIYLKAEVENPKTLPGMEWDVLSWWRLNSQKYPVLSEIARDVLAMQVSYVASESAF 154
Query: 58 SAGGRVVDPYRSSLTPKTLEALICTQDWIKGKYSKS---LLSNEEVME----LEKYEQG 109
S GR+++P RS LT +E L+C + W+K + S L+N +++ L+K E+G
Sbjct: 155 STSGRLLEPSRSCLTHYMVETLVCLEQWLKSEIKLSENTFLTNTQLLADIELLDKLEKG 213
>At1g42110 hypothetical protein
Length = 659
Score = 89.7 bits (221), Expect = 3e-19
Identities = 44/91 (48%), Positives = 67/91 (73%), Gaps = 4/91 (4%)
Query: 2 SKNELTKYLEDG----LEERGSLDILNWWKLNASRYPILASIARELLAIPISTVASESTF 57
+K+ L YL+D ++ ++IL++WK N RY LAS+A +LL+IPI+TVASES F
Sbjct: 536 TKSTLEIYLDDEPRLEMKTFSDMEILSFWKENQHRYGDLASMASDLLSIPITTVASESAF 595
Query: 58 SAGGRVVDPYRSSLTPKTLEALICTQDWIKG 88
S GGRV++P+R+ L P+ ++ALICT++W+ G
Sbjct: 596 SVGGRVLNPFRNRLLPQNVQALICTRNWLLG 626
>At3g45270 putative protein
Length = 661
Score = 89.4 bits (220), Expect = 4e-19
Identities = 44/118 (37%), Positives = 81/118 (68%), Gaps = 12/118 (10%)
Query: 3 KNELTKYLEDGLEERGSL-----DILNWWKLNASRYPILASIARELLAIPISTVASESTF 57
++EL YL++ +E ++ ++L+WWKLN R+P+L++I +++LA+ +S+VASES F
Sbjct: 537 RDELDVYLKEKVENPKTIIGLEWEVLSWWKLNCGRFPVLSAITKDVLAMQVSSVASESAF 596
Query: 58 SAGGRVVDPYRSSLTPKTLEALICTQDWIKGKY---SKSLLSNEE----VMELEKYEQ 108
S GRV++P+RS LT ++ L+CT+ W+K +KS+++ +E ++EL+ E+
Sbjct: 597 SNSGRVIEPHRSCLTHYMVKVLLCTEQWMKNVNHLGNKSVVTIKELLADIVELDNIEK 654
>At1g80020 hypothetical protein
Length = 1076
Score = 87.0 bits (214), Expect = 2e-18
Identities = 43/101 (42%), Positives = 61/101 (59%), Gaps = 1/101 (0%)
Query: 3 KNELTKYLEDGLEERGS-LDILNWWKLNASRYPILASIARELLAIPISTVASESTFSAGG 61
K+EL +YLE+ L R ++L WW LN ++YP L+ +A ++L++P TV+ +S F
Sbjct: 975 KSELDQYLEESLIPRSQDFEVLGWWSLNRTKYPTLSKMAADVLSVPFCTVSPDSVFDTEV 1034
Query: 62 RVVDPYRSSLTPKTLEALICTQDWIKGKYSKSLLSNEEVME 102
+ +D YRSSL TLEAL C +DW K S S N ME
Sbjct: 1035 KKMDNYRSSLRHVTLEALFCAKDWFKHSSSNSTSENNLKME 1075
>At5g39060 transposase -like protein
Length = 543
Score = 85.9 bits (211), Expect = 4e-18
Identities = 43/97 (44%), Positives = 67/97 (68%), Gaps = 3/97 (3%)
Query: 3 KNELTKYLEDGLEERGS---LDILNWWKLNASRYPILASIARELLAIPISTVASESTFSA 59
K+ L YLE+ + + S +D++ +WK N SR+ L+S+A ++L+IPI+TVASES FS
Sbjct: 419 KSPLDMYLEEPVLDMVSFRDMDVIAYWKNNVSRFKELSSMACDILSIPITTVASESAFSI 478
Query: 60 GGRVVDPYRSSLTPKTLEALICTQDWIKGKYSKSLLS 96
G RV++ YRS L P ++AL+CT++W +G K + S
Sbjct: 479 GSRVLNKYRSCLLPTNVQALLCTRNWFRGFQDKLMRS 515
>At1g59770 hypothetical protein
Length = 298
Score = 85.5 bits (210), Expect = 6e-18
Identities = 38/90 (42%), Positives = 66/90 (73%), Gaps = 3/90 (3%)
Query: 2 SKNELTKYLED---GLEERGSLDILNWWKLNASRYPILASIARELLAIPISTVASESTFS 58
+K+ L YL+D L +++L++WK + RY LAS+A +L+IPI+TVA+ES+FS
Sbjct: 181 TKSNLQNYLDDPRLDLRSFTDMEVLSYWKGDGQRYGDLASLASAILSIPITTVAAESSFS 240
Query: 59 AGGRVVDPYRSSLTPKTLEALICTQDWIKG 88
GGR+++P+R+ L + ++AL+CT++W++G
Sbjct: 241 IGGRILNPFRNRLLSRNVQALLCTRNWLRG 270
>At3g29120 hypothetical protein
Length = 154
Score = 84.7 bits (208), Expect = 1e-17
Identities = 41/89 (46%), Positives = 64/89 (71%), Gaps = 3/89 (3%)
Query: 3 KNELTKYLEDGLEERGS---LDILNWWKLNASRYPILASIARELLAIPISTVASESTFSA 59
K+ L YLE+ + + S +D++ +WK N SR+ L+S+A ++L+IPI+TVASES FS
Sbjct: 46 KSPLDMYLEEPVLDMVSFRDMDVIAYWKNNVSRFKELSSMACDILSIPITTVASESAFSI 105
Query: 60 GGRVVDPYRSSLTPKTLEALICTQDWIKG 88
G RV++ YRS L P ++AL+CT++W +G
Sbjct: 106 GSRVLNKYRSCLLPTNVQALLCTRNWFRG 134
>At1g15300 unknown protein
Length = 799
Score = 84.3 bits (207), Expect = 1e-17
Identities = 38/84 (45%), Positives = 60/84 (71%), Gaps = 1/84 (1%)
Query: 5 ELTKYLEDGLEERGS-LDILNWWKLNASRYPILASIARELLAIPISTVASESTFSAGGRV 63
EL +YLE+ L R ++L+WW++N + YP L+ +A +LL++P STV+ +S F +
Sbjct: 712 ELDQYLEENLIPRSEDFNVLSWWRVNNTNYPTLSKMAVDLLSVPFSTVSPDSVFDTEVKQ 771
Query: 64 VDPYRSSLTPKTLEALICTQDWIK 87
+D YR+SL +TLEAL+CT+DW+K
Sbjct: 772 MDNYRTSLPGETLEALLCTKDWLK 795
>At1g49550 hypothetical protein
Length = 258
Score = 83.6 bits (205), Expect = 2e-17
Identities = 47/109 (43%), Positives = 74/109 (67%), Gaps = 6/109 (5%)
Query: 3 KNELTKYLEDGLEERGSL---DILNWWKLNASRYPILASIARELLAIPISTVASESTFSA 59
K+EL YL + + + ++L +WK N+ R+ L+S+A ++L+IPI+TVASES+FS
Sbjct: 145 KSELDIYLGEPTLDMAAFRHFNVLAYWKDNSCRFKELSSMACDVLSIPITTVASESSFSI 204
Query: 60 GGRVVDPYRSSLTPKTLEALICTQDWIKGKYSKSLLSNEEVMELEKYEQ 108
G V+ YRSSL P+ ++ALICT++W++G + K EE +E EK E+
Sbjct: 205 GSGVLSKYRSSLLPENIQALICTRNWLRG-FPKE--GEEEEVEEEKEEE 250
>At3g42170 putative transposase
Length = 696
Score = 82.8 bits (203), Expect = 4e-17
Identities = 37/85 (43%), Positives = 59/85 (68%), Gaps = 1/85 (1%)
Query: 3 KNELTKYLEDGLEER-GSLDILNWWKLNASRYPILASIARELLAIPISTVASESTFSAGG 61
K+EL +YL++ L R D+L+WWK N +YP L+ +AR++L+IP+S A + F
Sbjct: 590 KSELDQYLDETLLPRVQEFDVLDWWKQNKLKYPTLSKMARDILSIPVSAAAFDYVFDMEP 649
Query: 62 RVVDPYRSSLTPKTLEALICTQDWI 86
R +D Y++SL P+T+EALIC ++W+
Sbjct: 650 REMDEYKTSLRPETVEALICAREWL 674
>At4g05510
Length = 604
Score = 80.9 bits (198), Expect = 1e-16
Identities = 43/102 (42%), Positives = 67/102 (65%), Gaps = 8/102 (7%)
Query: 3 KNELTKYLEDG---LEERGSLDILNWWKLNASRYPILASIARELLAIPISTVASESTFSA 59
K+ L YLED ++ +L++L +WK N R+ LA +A ++L+IPI++VASES+FS
Sbjct: 496 KSALDMYLEDPKLEMKNHPNLNVLQYWKENRLRFGALAYMAMDVLSIPITSVASESSFSI 555
Query: 60 GGRVVDPYRSSLTPKTLEALICTQDWIKGKYSKSLLSNEEVM 101
G V++ YRS L P ++AL+CT+ W+ G +S+EE M
Sbjct: 556 GSHVLNKYRSRLLPTNVQALLCTRSWLYG-----FVSDEEGM 592
>At1g69950 unknown protein
Length = 676
Score = 80.9 bits (198), Expect = 1e-16
Identities = 43/95 (45%), Positives = 61/95 (63%), Gaps = 3/95 (3%)
Query: 2 SKNELTKYLEDGLEERGS-LDILNWWKLNASRYPILASIARELLAIPISTVASESTF--S 58
SK++L KYLE+ L R S DILNWWK++ +YPIL+ +AR +LA+P+ V+SE +
Sbjct: 582 SKSDLEKYLEEPLFPRNSDFDILNWWKVHTPKYPILSMMARNVLAVPMLNVSSEEDAFET 641
Query: 59 AGGRVVDPYRSSLTPKTLEALICTQDWIKGKYSKS 93
R V SL P T++AL+C QDWI+ + S
Sbjct: 642 CQRRRVSETWRSLRPSTVQALMCAQDWIQSELESS 676
>At3g14800 unknown protein
Length = 662
Score = 77.0 bits (188), Expect = 2e-15
Identities = 36/83 (43%), Positives = 55/83 (65%), Gaps = 1/83 (1%)
Query: 5 ELTKYLEDGLEER-GSLDILNWWKLNASRYPILASIARELLAIPISTVASESTFSAGGRV 63
+L KYL + + R G +ILN+WK++ RYPIL+ +AR++L P+S A +STF++G V
Sbjct: 568 DLDKYLSEPIFPRSGEFNILNYWKVHTPRYPILSLLARDILGTPMSICAPDSTFNSGTPV 627
Query: 64 VDPYRSSLTPKTLEALICTQDWI 86
+ +SSL P +AL C DW+
Sbjct: 628 ISDSQSSLNPDIRQALFCAHDWL 650
>At2g18580 Ac-like transposase
Length = 308
Score = 77.0 bits (188), Expect = 2e-15
Identities = 39/93 (41%), Positives = 61/93 (64%), Gaps = 2/93 (2%)
Query: 19 SLDILNWWKLNASRYPILASIARELLAIPISTVASESTFSAGGRVVDPYRSSLTPKTLEA 78
SLD+L +WK N SR+ L+ + ++L IPI+T++SES+FS G +V++ Y+S L P ++A
Sbjct: 215 SLDVLKYWKDNGSRFKELSRMVCDVLCIPITTMSSESSFSVGSKVLNKYKSRLLPSNVQA 274
Query: 79 LICTQDWIKG--KYSKSLLSNEEVMELEKYEQG 109
LIC ++W+ G + S+S S E E E G
Sbjct: 275 LICARNWLHGFKEISESEFSEAREEEEEGEEGG 307
>At5g39070 putative protein
Length = 154
Score = 75.9 bits (185), Expect = 5e-15
Identities = 34/76 (44%), Positives = 57/76 (74%), Gaps = 1/76 (1%)
Query: 14 LEERGSLD-ILNWWKLNASRYPILASIARELLAIPISTVASESTFSAGGRVVDPYRSSLT 72
L RG+ + +L++WK + R+ LAS+ +L+IPI+TVA ES+FS GGR ++P+R+ L
Sbjct: 51 LSLRGAYNLVLSYWKGDGQRFGDLASLVSAILSIPITTVAVESSFSIGGRALNPFRNRLL 110
Query: 73 PKTLEALICTQDWIKG 88
P+ ++AL+CT++W+ G
Sbjct: 111 PRNVQALLCTKNWLHG 126
>At1g18560 hypothetical protein
Length = 676
Score = 71.6 bits (174), Expect = 9e-14
Identities = 36/104 (34%), Positives = 62/104 (59%), Gaps = 5/104 (4%)
Query: 4 NELTKYLEDGLEERGSLDILNWWKLNASRYPILASIARELLAIPISTVASESTFSAGGRV 63
+ELT+YL + + + D+L+WWK+N+ RYP L+++AR+ LA+ ++ A E F G
Sbjct: 554 DELTQYLSESIVPMQT-DVLDWWKVNSGRYPRLSNMARDFLAVQATSAAPEEIFCGKGEE 612
Query: 64 VDPYRSSLTPKTLEALICTQDWI----KGKYSKSLLSNEEVMEL 103
+D + + + +++IC + WI K KY S + E +MEL
Sbjct: 613 IDKQKYCMPHDSTQSVICIRSWIEAGMKLKYKCSEIDYERLMEL 656
>At4g13120 putative protein (possibly fragment)
Length = 770
Score = 70.5 bits (171), Expect = 2e-13
Identities = 37/66 (56%), Positives = 47/66 (71%), Gaps = 1/66 (1%)
Query: 37 ASIARELLAIPISTVASESTFSAGGRVVDPYRSSLTPKTLEALICTQDWIK-GKYSKSLL 95
A +AR++LAI +S+ A ES F GRV+DPYRSSLTP T EALICTQ W++ S+ L
Sbjct: 695 AELARDVLAIQMSSFAHESAFCTSGRVLDPYRSSLTPYTTEALICTQQWLRTSLQSEPPL 754
Query: 96 SNEEVM 101
+N E M
Sbjct: 755 ANLEQM 760
>At4g07540 putative transposon protein
Length = 483
Score = 70.5 bits (171), Expect = 2e-13
Identities = 32/60 (53%), Positives = 48/60 (79%)
Query: 29 NASRYPILASIARELLAIPISTVASESTFSAGGRVVDPYRSSLTPKTLEALICTQDWIKG 88
N R+ LAS+A ++L+IPI+TVASES+FS G RV+ YR+ L P+ ++ALIC+++W+KG
Sbjct: 416 NGPRFGKLASMACDILSIPITTVASESSFSIGTRVLSKYRNRLLPRNVQALICSRNWLKG 475
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.323 0.139 0.419
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,547,801
Number of Sequences: 26719
Number of extensions: 86610
Number of successful extensions: 240
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 34
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 203
Number of HSP's gapped (non-prelim): 45
length of query: 122
length of database: 11,318,596
effective HSP length: 87
effective length of query: 35
effective length of database: 8,994,043
effective search space: 314791505
effective search space used: 314791505
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 53 (25.0 bits)
Lotus: description of TM0185.2