BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0157b.4
(77 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g23090 unknown protein 139 3e-34
At5g03740 histone deacetylase -like protein 33 0.020
At1g03080 unknown protein 28 1.1
At3g02260 unknown protein 27 1.4
At4g28740 unknown protein 27 1.8
At1g18560 hypothetical protein 27 1.8
At5g61190 putative protein 27 2.4
At3g10680 hypothetical protein 27 2.4
At1g02040 hypothetical protein 26 3.1
At2g37580 putative RING zinc finger protein 26 4.1
At5g23800 putative protein 25 5.3
At1g07570 protein kinase APK1A 25 5.3
At4g00060 unknown protein 25 9.1
At1g65180 unknown protein (At1g65180) 25 9.1
>At2g23090 unknown protein
Length = 78
Score = 139 bits (350), Expect = 3e-34
Identities = 67/78 (85%), Positives = 71/78 (90%), Gaps = 1/78 (1%)
Query: 1 MGGGNAQKAKMARERNLEKQKGA-KGSQLETNKKAMSIQCKVCMQTFICTTSEVKCREHA 59
MGGGNAQK+ MAR +NLEK K A KGSQLE NKKAMSIQCKVCMQTFICTTSEVKCREHA
Sbjct: 1 MGGGNAQKSAMARAKNLEKAKAAGKGSQLEANKKAMSIQCKVCMQTFICTTSEVKCREHA 60
Query: 60 EAKHPKSDVVTCFPHLQK 77
EAKHPK+DVV CFPHL+K
Sbjct: 61 EAKHPKADVVACFPHLKK 78
>At5g03740 histone deacetylase -like protein
Length = 294
Score = 33.5 bits (75), Expect = 0.020
Identities = 19/55 (34%), Positives = 28/55 (50%), Gaps = 3/55 (5%)
Query: 9 AKMARERNLEKQKGAKGSQLETNKKAMSIQCKVCMQTFICTTSEVKCREHAEAKH 63
+K A + + G Q +T K A + CK C +TF TSE+ + H +AKH
Sbjct: 239 SKQAGKNSGGGSTGETSKQQQTPKSAGAFGCKSCTRTF---TSEMGLQSHTKAKH 290
>At1g03080 unknown protein
Length = 1744
Score = 27.7 bits (60), Expect = 1.1
Identities = 13/55 (23%), Positives = 23/55 (41%)
Query: 5 NAQKAKMARERNLEKQKGAKGSQLETNKKAMSIQCKVCMQTFICTTSEVKCREHA 59
N + ++R +E+ KG + + NK + CK F+ S +HA
Sbjct: 1353 NLESRSTLKDREIEQLKGRVNNLEDANKGQNDLMCKYAQAIFLLKESIQSLEKHA 1407
>At3g02260 unknown protein
Length = 5079
Score = 27.3 bits (59), Expect = 1.4
Identities = 17/57 (29%), Positives = 30/57 (51%), Gaps = 6/57 (10%)
Query: 4 GNAQKAKMARERNLEKQKGAKGSQLETNKKAMSIQCKVCMQTFICTTSEVKCREHAE 60
G+++K +A+ ++E+Q A G +L +SI VC SE+KC++ E
Sbjct: 2330 GSSKKRALAQSASMEEQVIADGLKL------LSIYYSVCRPRQEVVLSELKCKQLLE 2380
>At4g28740 unknown protein
Length = 347
Score = 26.9 bits (58), Expect = 1.8
Identities = 14/33 (42%), Positives = 21/33 (63%), Gaps = 4/33 (12%)
Query: 5 NAQKAKMARERNLEKQKGAKGSQLETNKKAMSI 37
NAQ A+++RE NL K K ++E N K +S+
Sbjct: 173 NAQMARLSREENLGKLK----MRVEENNKVISV 201
>At1g18560 hypothetical protein
Length = 676
Score = 26.9 bits (58), Expect = 1.8
Identities = 17/75 (22%), Positives = 31/75 (40%), Gaps = 5/75 (6%)
Query: 1 MGGGNAQKAKMARERNLEKQKGAKGSQLETNKKAMSIQCKVCMQTFICTTSEVKCREHAE 60
+G G++ K+ + + ET + + +CK C Q++ T+ H
Sbjct: 20 IGLGSSDKSNSVPPKRKKTMTSVYLKYFETAPDSKTRKCKFCGQSYSIATATGNLGRHLT 79
Query: 61 AKHP-----KSDVVT 70
+HP +DVVT
Sbjct: 80 NRHPGYDKAAADVVT 94
>At5g61190 putative protein
Length = 976
Score = 26.6 bits (57), Expect = 2.4
Identities = 14/56 (25%), Positives = 29/56 (51%)
Query: 4 GNAQKAKMARERNLEKQKGAKGSQLETNKKAMSIQCKVCMQTFICTTSEVKCREHA 59
G A+ +K E++ +K S+ + N + + + C V Q+ I S ++ ++HA
Sbjct: 338 GPAEPSKEVLEKHNMNKKVMIESRAQANAEFVCLMCNVVCQSQIVFNSHLRGKKHA 393
>At3g10680 hypothetical protein
Length = 490
Score = 26.6 bits (57), Expect = 2.4
Identities = 17/67 (25%), Positives = 31/67 (45%), Gaps = 4/67 (5%)
Query: 1 MGGGNAQKAKMARERNLEKQKGAKGSQLETNKKAMSIQCKVCMQTFICTTSEVKCREHAE 60
+GG K + E+ +E++K SQL+T +K + + + V+ +EHA+
Sbjct: 248 LGGSLKPKVQAKEEKVIERKKDDDISQLKTGQKVKEKE----ISRTPTIDARVESKEHAK 303
Query: 61 AKHPKSD 67
K D
Sbjct: 304 VVEKKED 310
>At1g02040 hypothetical protein
Length = 324
Score = 26.2 bits (56), Expect = 3.1
Identities = 22/60 (36%), Positives = 25/60 (41%), Gaps = 7/60 (11%)
Query: 19 KQKG-AKGSQLETNKKAMSIQCKVCMQTFICTTSEVKCREHAEAKHPKSDVVTCFPHLQK 77
KQ G KG ++ K A QCK C + F TS H A H K V CF K
Sbjct: 131 KQHGHGKGKTVKKQKTAQVFQCKACKKVF---TSHQALGGH-RASHKK--VKGCFASQDK 184
>At2g37580 putative RING zinc finger protein
Length = 630
Score = 25.8 bits (55), Expect = 4.1
Identities = 14/50 (28%), Positives = 25/50 (50%), Gaps = 4/50 (8%)
Query: 14 ERNLEKQKGAKGSQLETNKKAMSIQCKVCMQTFICTTSEVKCREHAEAKH 63
E + + G K + +T+ K + +C VC+ F T + R+ +E KH
Sbjct: 118 ETTVTSESGGKFHK-DTHSKEIGNECSVCLMVF---TDSDELRQLSECKH 163
>At5g23800 putative protein
Length = 552
Score = 25.4 bits (54), Expect = 5.3
Identities = 12/35 (34%), Positives = 17/35 (48%)
Query: 43 MQTFICTTSEVKCREHAEAKHPKSDVVTCFPHLQK 77
M TF C +VK +H EA+ + F L+K
Sbjct: 417 MLTFSCLLEQVKALQHDEARSSFISLSNSFAELEK 451
>At1g07570 protein kinase APK1A
Length = 410
Score = 25.4 bits (54), Expect = 5.3
Identities = 14/43 (32%), Positives = 22/43 (50%), Gaps = 6/43 (13%)
Query: 34 AMSIQCKVCMQTFICTTSEVKCREHAEAKHPKSDVVTCFPHLQ 76
+M CKV + C T+E+K R + S+VV+ H+Q
Sbjct: 318 SMEEACKVATLSLRCLTTEIKLRPN------MSEVVSHLEHIQ 354
>At4g00060 unknown protein
Length = 1344
Score = 24.6 bits (52), Expect = 9.1
Identities = 10/18 (55%), Positives = 10/18 (55%)
Query: 56 REHAEAKHPKSDVVTCFP 73
REH K P S VV C P
Sbjct: 836 REHPRIKRPPSPVVLCVP 853
>At1g65180 unknown protein (At1g65180)
Length = 653
Score = 24.6 bits (52), Expect = 9.1
Identities = 12/44 (27%), Positives = 20/44 (45%)
Query: 13 RERNLEKQKGAKGSQLETNKKAMSIQCKVCMQTFICTTSEVKCR 56
+E N K K L TN + S++C+ C +T +C+
Sbjct: 56 KEINHPSHKCDKNLSLITNFASWSVRCRTCKETIQPYIQNYECQ 99
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.315 0.125 0.368
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,675,853
Number of Sequences: 26719
Number of extensions: 49635
Number of successful extensions: 177
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 169
Number of HSP's gapped (non-prelim): 14
length of query: 77
length of database: 11,318,596
effective HSP length: 53
effective length of query: 24
effective length of database: 9,902,489
effective search space: 237659736
effective search space used: 237659736
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)
Lotus: description of TM0157b.4