BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0142.9
(341 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g15310 unknown protein 224 5e-59
At1g42690 unknown protein 166 2e-41
At1g24370 hypothetical protein 149 3e-36
At2g13770 hypothetical protein 88 8e-18
At5g28700 putative protein 82 3e-16
At2g07520 pseudogene 75 5e-14
At2g14730 hypothetical protein 65 4e-11
At5g12010 putative protein 42 4e-04
At4g29780 unknown protein 32 0.38
At1g36675 putative protein 31 1.1
At1g47550 hypothetical protein 29 3.2
At5g54250 cyclic nucleotide and calmodulin-regulated ion channel 28 5.5
At5g48740 receptor protein kinase-like protein 28 9.4
>At3g15310 unknown protein
Length = 415
Score = 224 bits (571), Expect = 5e-59
Identities = 106/191 (55%), Positives = 136/191 (70%), Gaps = 1/191 (0%)
Query: 1 RKYLDRDHAAANQRLINDYFANEPTYDDAMFRRRYRMQKHLFLRIVDDLSSSDNYFTQRV 60
R Y DR + L NDYF++ + FRRR+RM+K LFLRIVD LSS +F R
Sbjct: 45 RAYFDRKREEGHVLLWNDYFSDNAIFPLQTFRRRFRMKKPLFLRIVDRLSSELMFFQHRR 104
Query: 61 DAANK*GISPLAKCTTAIRMLAYGVAAHAVDEYIKIGGTTSLECLRRFCKGIIRLYEQQY 120
DA + G SP+ KCT AIR+LAYG A+ AVDEY+++G TT++ CL F KGII + +Y
Sbjct: 105 DATGRFGHSPIQKCTAAIRLLAYGYASDAVDEYLRMGETTAMSCLENFTKGIISFFGDEY 164
Query: 121 LRAPTQEDLQRILHASDMRGFPGMIESIDYMHWEWKNCPKAWEGQFTRGDKGTTTVILEA 180
LRAPT +L+R+L+ +RGFPGMI S+D MHWEWKNCP AW+GQ+TRG G T++LEA
Sbjct: 165 LRAPTATNLRRLLNIGKIRGFPGMIGSLDCMHWEWKNCPTAWKGQYTRG-SGKPTIVLEA 223
Query: 181 VASHDLWIWHI 191
+AS DLWIWH+
Sbjct: 224 IASQDLWIWHV 234
>At1g42690 unknown protein
Length = 333
Score = 166 bits (419), Expect = 2e-41
Identities = 86/178 (48%), Positives = 107/178 (59%), Gaps = 31/178 (17%)
Query: 3 YLDRDHAAANQRLINDYFANEPTYDDAMFRRRYRMQKHLFLRIVDDLSSSDNYFTQRVDA 62
Y++R+ + +L+NDYF PTY +FRRR+RM K LF+RIV+ S+ YF QR DA
Sbjct: 43 YIERNREEGHIQLVNDYFTENPTYPPHIFRRRFRMNKSLFMRIVERFSNEVPYFKQRRDA 102
Query: 63 ANK*GISPLAKCTTAIRMLAYGVAAHAVDEYIKIGGTTSLECLRRFCKGIIRLYEQQYLR 122
+ G S L K T AIRMLAYG+AA A YLR
Sbjct: 103 TRRLGFSALQKSTAAIRMLAYGIAADA------------------------------YLR 132
Query: 123 APTQEDLQRILHASDMRGFPGMIESIDYMHWEWKNCPKAWEGQFTRGDKGTTTVILEA 180
PT++DL R+LH + RGFPGMI SID MHWEWKNCP AW+GQ+TRG G T++LEA
Sbjct: 133 RPTRDDLIRLLHIGEQRGFPGMIGSIDCMHWEWKNCPTAWKGQYTRG-SGKPTIVLEA 189
>At1g24370 hypothetical protein
Length = 413
Score = 149 bits (375), Expect = 3e-36
Identities = 66/116 (56%), Positives = 86/116 (73%), Gaps = 1/116 (0%)
Query: 75 TTAIRMLAYGVAAHAVDEYIKIGGTTSLECLRRFCKGIIRLYEQQYLRAPTQEDLQRILH 134
T A RMLAYGV A + DEYIKIG +T+LE L+RFC+ I+ ++ +YLR+P D+ R+LH
Sbjct: 2 TAAFRMLAYGVPADSTDEYIKIGESTALESLKRFCRAIVEVFACRYLRSPDANDVARLLH 61
Query: 135 ASDMRGFPGMIESIDYMHWEWKNCPKAWEGQFTRGDKGTTTVILEAVASHDLWIWH 190
+ RGFP M+ S+D MHW+WKNCP AW GQ+ G + T+ILEAVA +DLWIWH
Sbjct: 62 IGESRGFPRMLGSLDCMHWKWKNCPTAWGGQYA-GRSRSPTIILEAVADYDLWIWH 116
>At2g13770 hypothetical protein
Length = 244
Score = 87.8 bits (216), Expect = 8e-18
Identities = 47/116 (40%), Positives = 60/116 (51%), Gaps = 36/116 (31%)
Query: 75 TTAIRMLAYGVAAHAVDEYIKIGGTTSLECLRRFCKGIIRLYEQQYLRAPTQEDLQRILH 134
T A RMLAYGV A + DEYIKIG +T+LE L+RFC+ I+ ++ YLR+P D+ R+LH
Sbjct: 2 TAAFRMLAYGVPADSTDEYIKIGESTALESLKRFCRAIVEVFACCYLRSPDANDVARLLH 61
Query: 135 ASDMRGFPGMIESIDYMHWEWKNCPKAWEGQFTRGDKGTTTVILEAVASHDLWIWH 190
+ RGFP EAVA +DLWIWH
Sbjct: 62 IGESRGFP------------------------------------EAVADYDLWIWH 81
>At5g28700 putative protein
Length = 292
Score = 82.4 bits (202), Expect = 3e-16
Identities = 43/78 (55%), Positives = 53/78 (67%)
Query: 25 TYDDAMFRRRYRMQKHLFLRIVDDLSSSDNYFTQRVDAANK*GISPLAKCTTAIRMLAYG 84
TY +FR R+RM K LF+RIV+ S+ YF QR DA + S L K T AIRMLAYG
Sbjct: 33 TYPPHIFRHRFRMNKPLFMRIVERFSNEVPYFKQRRDATGRLDFSALQKSTAAIRMLAYG 92
Query: 85 VAAHAVDEYIKIGGTTSL 102
+AA AVDEY++IG +T L
Sbjct: 93 IAADAVDEYLRIGESTLL 110
Score = 28.1 bits (61), Expect = 7.2
Identities = 12/21 (57%), Positives = 16/21 (76%), Gaps = 2/21 (9%)
Query: 172 GTTTVILEAVASHDLWIWHII 192
G +T+++ VAS DLWIWH I
Sbjct: 105 GESTLLV--VASQDLWIWHAI 123
>At2g07520 pseudogene
Length = 222
Score = 75.1 bits (183), Expect = 5e-14
Identities = 39/82 (47%), Positives = 49/82 (59%), Gaps = 12/82 (14%)
Query: 156 KNCPKAWEGQFTRGDKGTTTVILEAVASHDLWIWHII*PMVSILLIQLSSKRLDFLKVNP 215
KNCP AWEGQFTRGDKGT+T+ EAVASHDLWIWH S L ++FL +P
Sbjct: 16 KNCPIAWEGQFTRGDKGTSTISFEAVASHDLWIWHTFFGCASTL------NDINFLDRSP 69
Query: 216 ISYLQKFRRDVERTSNVHLEFF 237
+ D+E+ + + FF
Sbjct: 70 VF------DDIEQGNTPRVNFF 85
>At2g14730 hypothetical protein
Length = 117
Score = 65.5 bits (158), Expect = 4e-11
Identities = 29/62 (46%), Positives = 44/62 (70%)
Query: 88 HAVDEYIKIGGTTSLECLRRFCKGIIRLYEQQYLRAPTQEDLQRILHASDMRGFPGMIES 147
HAVD+Y++IG T + C+ + II L+ ++YLR PT++DL+R+L ++RGF GM S
Sbjct: 18 HAVDKYLRIGENTLMSCMIHSVEAIIYLFGKEYLRRPTRQDLKRLLRIGELRGFLGMTGS 77
Query: 148 ID 149
ID
Sbjct: 78 ID 79
>At5g12010 putative protein
Length = 502
Score = 42.4 bits (98), Expect = 4e-04
Identities = 33/128 (25%), Positives = 56/128 (42%), Gaps = 5/128 (3%)
Query: 26 YDDAMFRRRYRMQKHLFLRIVDDLSSSDNYFTQRVDAANK*GISPLAKCTTAIRMLAYGV 85
Y + F++ +RM K F I D+L+S+ + D A + I + I LA G
Sbjct: 170 YPEEDFKKAFRMSKSTFELICDELNSA----VAKEDTALRNAIPVRQRVAVCIWRLATGE 225
Query: 86 AAHAVDEYIKIGGTTSLECLRRFCKGIIRLYEQQYLRAPTQEDLQRILHA-SDMRGFPGM 144
V + +G +T + + CK I + +YL+ P E L+ I + G P +
Sbjct: 226 PLRLVSKKFGLGISTCHKLVLEVCKAIKDVLMPKYLQWPDDESLRNIRERFESVSGIPNV 285
Query: 145 IESIDYMH 152
+ S+ H
Sbjct: 286 VGSMYTTH 293
>At4g29780 unknown protein
Length = 540
Score = 32.3 bits (72), Expect = 0.38
Identities = 23/108 (21%), Positives = 46/108 (42%), Gaps = 4/108 (3%)
Query: 22 NEPTYDDAMFRRRYRMQKHLFLRIVDDLSSSDNYFTQRVDAANK*GISPLAKCTTAIRML 81
+ P + + FRR +RM K F I ++L ++ + + + I + + L
Sbjct: 204 SRPDFPEDEFRREFRMSKSTFNLICEELDTT----VTKKNTMLRDAIPAPKRVGVCVWRL 259
Query: 82 AYGVAAHAVDEYIKIGGTTSLECLRRFCKGIIRLYEQQYLRAPTQEDL 129
A G V E +G +T + + C+ I + +YL P+ ++
Sbjct: 260 ATGAPLRHVSERFGLGISTCHKLVIEVCRAIYDVLMPKYLLWPSDSEI 307
>At1g36675 putative protein
Length = 268
Score = 30.8 bits (68), Expect = 1.1
Identities = 13/18 (72%), Positives = 15/18 (83%)
Query: 48 DLSSSDNYFTQRVDAANK 65
DLSSSDNYFT+R +A K
Sbjct: 175 DLSSSDNYFTKRFEATKK 192
>At1g47550 hypothetical protein
Length = 861
Score = 29.3 bits (64), Expect = 3.2
Identities = 20/77 (25%), Positives = 30/77 (37%)
Query: 7 DHAAANQRLINDYFANEPTYDDAMFRRRYRMQKHLFLRIVDDLSSSDNYFTQRVDAANK* 66
D AN R + + A RR ++ F +VD + S +YF+QR
Sbjct: 362 DPIYANMRAVKEKRAELEKLKATFVRRASEFLRNYFASLVDFMVSDKSYFSQRGQLKRPD 421
Query: 67 GISPLAKCTTAIRMLAY 83
KC T R+L +
Sbjct: 422 HADLRYKCRTYARLLQH 438
>At5g54250 cyclic nucleotide and calmodulin-regulated ion channel
Length = 694
Score = 28.5 bits (62), Expect = 5.5
Identities = 15/38 (39%), Positives = 22/38 (57%), Gaps = 1/38 (2%)
Query: 77 AIRMLAYGVAAHAVDE-YIKIGGTTSLECLRRFCKGII 113
A+ M+AY VAAHA + +G S +CL+ C+ I
Sbjct: 262 ALNMIAYFVAAHAAGACWYLLGVQRSAKCLKEQCENTI 299
>At5g48740 receptor protein kinase-like protein
Length = 879
Score = 27.7 bits (60), Expect = 9.4
Identities = 40/170 (23%), Positives = 82/170 (47%), Gaps = 37/170 (21%)
Query: 82 AYGVAAHAVDEYIKIGGTTSLECLRRFCKG------IIRLYE----QQYLRAPTQEDLQR 131
AY ++ + ++YI+ G TT++ + +G IRL+ +Q + P ++DL
Sbjct: 34 AYNISWVSDNDYIETGNTTTV----TYAEGNSTSSVPIRLFPDPQGRQCYKLPVRKDLSS 89
Query: 132 ILHASDMRGFPGMIESIDYMHWEWKNCPKAWEGQFTRGDKGTTTVILEAVASHDLWIWHI 191
+L + + Y +++ +N P A+ + G + T+TV L ++D WI +
Sbjct: 90 VL----------IRATFVYRNYDSQNSPPAF--HVSLGRRITSTVDLR---TNDPWIEEL 134
Query: 192 I*PM--VSILLIQLSSKR-----LDFLKVNPISYLQKFRRDVERTSNVHL 234
+ P+ S+LL L+ K + L+V P+ L ++ +E + ++ L
Sbjct: 135 VWPVNNDSLLLCLLAVKGRGIPVISSLEVRPLP-LGSYKYSLEGSPDIIL 183
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.340 0.148 0.484
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,853,945
Number of Sequences: 26719
Number of extensions: 260346
Number of successful extensions: 806
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 791
Number of HSP's gapped (non-prelim): 16
length of query: 341
length of database: 11,318,596
effective HSP length: 100
effective length of query: 241
effective length of database: 8,646,696
effective search space: 2083853736
effective search space used: 2083853736
T: 11
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.9 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0142.9