BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0136.17
(83 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g26430 nodulin, putative 82 5e-17
At5g14450 early nodule-specific protein - like 82 6e-17
At1g67830 ENOD8-like protein 82 6e-17
At4g01130 putative acetyltransferase 72 4e-14
At3g27950 early nodule-specific protein, putative 68 9e-13
At1g28570 lipase like protein 65 6e-12
At2g27360 putative lipase 62 5e-11
At1g54790 62 5e-11
At1g28590 putative lipase (At1g28590) 60 2e-10
At1g28650 lipase, putative 60 3e-10
At1g28640 lipase, putative 59 3e-10
At1g31550 unknown protein 59 6e-10
At3g62280 putative protein 57 2e-09
At1g28660 unknown protein 57 2e-09
At3g05180 putative nodulin 55 5e-09
At1g56670 lipase homolog (Lip-4) 55 5e-09
At1g28600 lipase like protein 55 8e-09
At1g28580 lipase like protein 54 1e-08
At1g09390 putative lipase 54 1e-08
At1g28670 lipase 54 1e-08
>At3g26430 nodulin, putative
Length = 380
Score = 82.0 bits (201), Expect = 5e-17
Identities = 37/67 (55%), Positives = 48/67 (71%)
Query: 17 VILFTTTLNPVFAKKRCNFPAIFNLGASNSDTGGLAAAFLPPKSPYGDTYFHRPAGRYSD 76
V+L + ++P CNFPAIFN G SNSDTGGL+A+F P G T+FH P+GR+SD
Sbjct: 11 VLLASCLIHPRACSPSCNFPAIFNFGDSNSDTGGLSASFGQAPYPNGQTFFHSPSGRFSD 70
Query: 77 GRIILDF 83
GR+I+DF
Sbjct: 71 GRLIIDF 77
>At5g14450 early nodule-specific protein - like
Length = 389
Score = 81.6 bits (200), Expect = 6e-17
Identities = 39/72 (54%), Positives = 49/72 (67%), Gaps = 1/72 (1%)
Query: 12 FVSLFVILFTTTLNPVFAKKRCNFPAIFNLGASNSDTGGLAAAFLPPKSPYGDTYFHRPA 71
F L + LF T + V + C FPAI+N G SNSDTGG++AAF P + PYG +FHRP
Sbjct: 17 FSWLLLCLFAVTTS-VSVQPTCTFPAIYNFGDSNSDTGGISAAFEPIRDPYGQGFFHRPT 75
Query: 72 GRYSDGRIILDF 83
GR SDGR+ +DF
Sbjct: 76 GRDSDGRLTIDF 87
>At1g67830 ENOD8-like protein
Length = 372
Score = 81.6 bits (200), Expect = 6e-17
Identities = 37/70 (52%), Positives = 50/70 (70%)
Query: 14 SLFVILFTTTLNPVFAKKRCNFPAIFNLGASNSDTGGLAAAFLPPKSPYGDTYFHRPAGR 73
SLF + ++L+P +C+FPAIFN G SNSDTGGL+AAF P+G ++F PAGR
Sbjct: 7 SLFALSLLSSLSPSTHAHQCHFPAIFNFGDSNSDTGGLSAAFGQAGPPHGSSFFGSPAGR 66
Query: 74 YSDGRIILDF 83
Y DGR+++DF
Sbjct: 67 YCDGRLVIDF 76
>At4g01130 putative acetyltransferase
Length = 382
Score = 72.4 bits (176), Expect = 4e-14
Identities = 33/52 (63%), Positives = 39/52 (74%)
Query: 32 RCNFPAIFNLGASNSDTGGLAAAFLPPKSPYGDTYFHRPAGRYSDGRIILDF 83
+C+F AIFN G SNSDTGG AAF P+G TYF +PAGR SDGR+I+DF
Sbjct: 29 KCDFEAIFNFGDSNSDTGGFWAAFPAQSGPWGMTYFKKPAGRASDGRLIIDF 80
>At3g27950 early nodule-specific protein, putative
Length = 361
Score = 67.8 bits (164), Expect = 9e-13
Identities = 33/71 (46%), Positives = 44/71 (61%)
Query: 13 VSLFVILFTTTLNPVFAKKRCNFPAIFNLGASNSDTGGLAAAFLPPKSPYGDTYFHRPAG 72
+++ V+L T CNFPA+FN G SNSDTG ++AA P G +F R AG
Sbjct: 8 LAIIVLLLGFTEKLSALSSSCNFPAVFNFGDSNSDTGAISAAIGEVPPPNGVAFFGRSAG 67
Query: 73 RYSDGRIILDF 83
R+SDGR+I+DF
Sbjct: 68 RHSDGRLIIDF 78
>At1g28570 lipase like protein
Length = 389
Score = 65.1 bits (157), Expect = 6e-12
Identities = 37/81 (45%), Positives = 51/81 (62%), Gaps = 7/81 (8%)
Query: 10 VSFVSLFVILFTTTLNPVFAKKRC-NFPAIFNLGASNSDTGGLAAAFLP---PKS---PY 62
+ VS F+IL T L V ++ +C NF +I + G S +DTG L A P PK PY
Sbjct: 6 MKLVSFFLILSTFCLTTVNSEPQCHNFKSIISFGDSIADTGNLLALSDPTNLPKVAFLPY 65
Query: 63 GDTYFHRPAGRYSDGRIILDF 83
G+T+FH P GR+S+GR+I+DF
Sbjct: 66 GETFFHHPTGRFSNGRLIIDF 86
>At2g27360 putative lipase
Length = 394
Score = 62.0 bits (149), Expect = 5e-11
Identities = 35/78 (44%), Positives = 50/78 (63%), Gaps = 9/78 (11%)
Query: 13 VSLFVILFTTTLNPVFAKKRC-NFPAIFNLGASNSDTGGLAAAFLP---PKS---PYGDT 65
+S F+ F T+ V ++ RC NF +I + G S +DTG L P P+S PYG+T
Sbjct: 10 LSFFISTFLITV--VTSQTRCRNFKSIISFGDSITDTGNLLGLSSPNDLPESAFPPYGET 67
Query: 66 YFHRPAGRYSDGRIILDF 83
+FH P+GR+SDGR+I+DF
Sbjct: 68 FFHHPSGRFSDGRLIIDF 85
>At1g54790
Length = 383
Score = 62.0 bits (149), Expect = 5e-11
Identities = 32/75 (42%), Positives = 46/75 (60%), Gaps = 1/75 (1%)
Query: 10 VSFVSLFVILFTTTLNPVFAKKRCNFPAIFNLGASNSDTGGLAAAFLPPKSP-YGDTYFH 68
V+ +S+ + + + P +PAI N G SNSDTG L +A + +P YG TYF+
Sbjct: 5 VNNLSIVLFILISLFLPSSFSIILKYPAIINFGDSNSDTGNLISAGIENVNPPYGQTYFN 64
Query: 69 RPAGRYSDGRIILDF 83
P+GRY DGR+I+DF
Sbjct: 65 LPSGRYCDGRLIVDF 79
>At1g28590 putative lipase (At1g28590)
Length = 403
Score = 60.1 bits (144), Expect = 2e-10
Identities = 34/73 (46%), Positives = 45/73 (61%), Gaps = 7/73 (9%)
Query: 18 ILFTTTLNPVFAKKRC-NFPAIFNLGASNSDTGGLAAAFLP---PKS---PYGDTYFHRP 70
IL T + V ++ +C NF +I + G S +DTG L P P S PYG+T+FH P
Sbjct: 16 ILSTLLVTSVNSQTQCRNFKSIISFGDSIADTGNLLGLSDPNDLPASAFPPYGETFFHHP 75
Query: 71 AGRYSDGRIILDF 83
GRYSDGR+I+DF
Sbjct: 76 TGRYSDGRLIIDF 88
>At1g28650 lipase, putative
Length = 385
Score = 59.7 bits (143), Expect = 3e-10
Identities = 33/82 (40%), Positives = 49/82 (59%), Gaps = 15/82 (18%)
Query: 13 VSLFVILFTTTLNPVFAKKRCN-FPAIFNLGASNSDTGGLA----------AAFLPPKSP 61
VS +IL+ TT+ ++ RC + +I + G S +DTG AAFLP
Sbjct: 12 VSFLLILYYTTIVVASSESRCRRYKSIISFGDSIADTGNYVHLSNVNNLPQAAFLP---- 67
Query: 62 YGDTYFHRPAGRYSDGRIILDF 83
YG+++FH P+GRYSDGR+++DF
Sbjct: 68 YGESFFHPPSGRYSDGRLVIDF 89
>At1g28640 lipase, putative
Length = 390
Score = 59.3 bits (142), Expect = 3e-10
Identities = 31/77 (40%), Positives = 48/77 (62%), Gaps = 7/77 (9%)
Query: 14 SLFVILFTTTLNPVFAKKRCN-FPAIFNLGASNSDTGGLA----AAFLPPKS--PYGDTY 66
S ++L++TT+ ++ RC F +I + G S +DTG LP + PYG+++
Sbjct: 11 SFLLVLYSTTIIVASSESRCRRFKSIISFGDSIADTGNYLHLSDVNHLPQSAFLPYGESF 70
Query: 67 FHRPAGRYSDGRIILDF 83
FH P+GRYSDGR+I+DF
Sbjct: 71 FHPPSGRYSDGRLIIDF 87
>At1g31550 unknown protein
Length = 394
Score = 58.5 bits (140), Expect = 6e-10
Identities = 36/90 (40%), Positives = 54/90 (60%), Gaps = 9/90 (10%)
Query: 1 MSLMEFHRHVSFVSLFVILFTTTLNPVFAKKRC-NFPAIFNLGASNSDTGGLAAAF---- 55
M+ ++ H + SLF L T ++ V ++ +C NF +I + G S +DTG L
Sbjct: 1 MASLDSHVLMKLGSLF--LSTLFVSIVSSESQCRNFESIISFGDSIADTGNLLGLSDHNN 58
Query: 56 LPPKS--PYGDTYFHRPAGRYSDGRIILDF 83
LP + PYG+T+FH P GR+SDGR+I+DF
Sbjct: 59 LPMSAFPPYGETFFHHPTGRFSDGRLIIDF 88
>At3g62280 putative protein
Length = 343
Score = 56.6 bits (135), Expect = 2e-09
Identities = 28/49 (57%), Positives = 33/49 (67%), Gaps = 1/49 (2%)
Query: 36 PAIFNLGASNSDTGG-LAAAFLPPKSPYGDTYFHRPAGRYSDGRIILDF 83
P + N G SNSDTGG LA LP P+G T+FHR GR DGR+I+DF
Sbjct: 35 PILINFGDSNSDTGGVLAGVGLPIGLPHGITFFHRGTGRLGDGRLIVDF 83
>At1g28660 unknown protein
Length = 382
Score = 56.6 bits (135), Expect = 2e-09
Identities = 30/77 (38%), Positives = 48/77 (61%), Gaps = 7/77 (9%)
Query: 14 SLFVILFTTTLNPVFAKKRCN-FPAIFNLGASNSDTGGLA----AAFLPPKS--PYGDTY 66
S ++L++TT+ ++ RC F +I + G S +DTG + LP + PYG+++
Sbjct: 11 SFLLVLYSTTIIVASSESRCRRFTSIISFGDSIADTGNILHLSDVNHLPQTAFFPYGESF 70
Query: 67 FHRPAGRYSDGRIILDF 83
FH P+GR SDGR+I+DF
Sbjct: 71 FHPPSGRASDGRLIIDF 87
>At3g05180 putative nodulin
Length = 379
Score = 55.5 bits (132), Expect = 5e-09
Identities = 36/85 (42%), Positives = 50/85 (58%), Gaps = 6/85 (7%)
Query: 4 MEFHRHVSFVSLFVILFTTTLNPVFAKKRCN--FPAIFNLGASNSDTGGLAA--AFLPPK 59
ME H L + + TL+P+ R + FPA+FN G SNSDTG L++ FLP
Sbjct: 1 METLFHTLLRLLLFVAISHTLSPLAGSFRISNDFPAVFNFGDSNSDTGELSSGLGFLPQP 60
Query: 60 SPYGDTYFHRP-AGRYSDGRIILDF 83
S Y T+F P +GR+ +GR+I+DF
Sbjct: 61 S-YEITFFRSPTSGRFCNGRLIVDF 84
>At1g56670 lipase homolog (Lip-4)
Length = 373
Score = 55.5 bits (132), Expect = 5e-09
Identities = 31/73 (42%), Positives = 38/73 (51%), Gaps = 1/73 (1%)
Query: 12 FVSLFVILFTTTLNPVFAKKRCNFPAIFNLGASNSDTGGLAAAF-LPPKSPYGDTYFHRP 70
F++L + P A P IFN G SNSDTGGL A P P G +F R
Sbjct: 15 FITLVSLALLILRQPSRAASCTARPVIFNFGDSNSDTGGLVAGLGYPIGFPNGRLFFRRS 74
Query: 71 AGRYSDGRIILDF 83
GR SDGR+++DF
Sbjct: 75 TGRLSDGRLLIDF 87
>At1g28600 lipase like protein
Length = 393
Score = 54.7 bits (130), Expect = 8e-09
Identities = 34/81 (41%), Positives = 46/81 (55%), Gaps = 8/81 (9%)
Query: 11 SFVSLFVILFTTTLNPVFAKKR-C-NFPAIFNLGASNSDTGGLAAAF----LPPKS--PY 62
S SL + LF+T + + + C NF +I + G S +DTG L LP + PY
Sbjct: 3 SLDSLVIFLFSTLFVTIVSSETPCPNFKSIISFGDSIADTGNLVGLSDRNQLPVTAFPPY 62
Query: 63 GDTYFHRPAGRYSDGRIILDF 83
G+T+FH P GR DGRII+DF
Sbjct: 63 GETFFHHPTGRSCDGRIIMDF 83
>At1g28580 lipase like protein
Length = 390
Score = 54.3 bits (129), Expect = 1e-08
Identities = 28/76 (36%), Positives = 43/76 (55%), Gaps = 7/76 (9%)
Query: 15 LFVILFTTTLNPVFAKKRCN-FPAIFNLGASNSDTGGLAAAFLPPK------SPYGDTYF 67
+F+ L T + V ++ +C F +I + G S +DTG L P PYG+ +F
Sbjct: 14 VFIFLSTFVVTNVSSETKCREFKSIISFGDSIADTGNLLGLSDPKDLPHMAFPPYGENFF 73
Query: 68 HRPAGRYSDGRIILDF 83
H P GR+S+GR+I+DF
Sbjct: 74 HHPTGRFSNGRLIIDF 89
>At1g09390 putative lipase
Length = 370
Score = 54.3 bits (129), Expect = 1e-08
Identities = 32/84 (38%), Positives = 41/84 (48%), Gaps = 1/84 (1%)
Query: 1 MSLMEFHRHVSFVSLFVILFTTTLNPVFAKKRCNFPAIFNLGASNSDTGGLAAAF-LPPK 59
M+ + H H + L + N A P IFN G SNSDTGGL A
Sbjct: 1 MATLSLHSHSFLLVLLPFILILRQNLAVAGGCQVPPVIFNFGDSNSDTGGLVAGLGYSIG 60
Query: 60 SPYGDTYFHRPAGRYSDGRIILDF 83
P G ++F R GR SDGR+++DF
Sbjct: 61 LPNGRSFFQRSTGRLSDGRLVIDF 84
>At1g28670 lipase
Length = 384
Score = 53.9 bits (128), Expect = 1e-08
Identities = 29/77 (37%), Positives = 47/77 (60%), Gaps = 7/77 (9%)
Query: 14 SLFVILFTTTLNPVFAKKRCN-FPAIFNLGASNSDTGGLA----AAFLPPKS--PYGDTY 66
S ++L++TT+ ++ RC F +I + G S +DTG LP + PYG+++
Sbjct: 11 SFLLVLYSTTIIVASSESRCRRFKSIISFGDSIADTGNYLHLSDVNHLPQSAFLPYGESF 70
Query: 67 FHRPAGRYSDGRIILDF 83
FH P+GR S+GR+I+DF
Sbjct: 71 FHPPSGRASNGRLIIDF 87
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.328 0.143 0.446
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,979,569
Number of Sequences: 26719
Number of extensions: 72791
Number of successful extensions: 263
Number of sequences better than 10.0: 82
Number of HSP's better than 10.0 without gapping: 47
Number of HSP's successfully gapped in prelim test: 35
Number of HSP's that attempted gapping in prelim test: 192
Number of HSP's gapped (non-prelim): 83
length of query: 83
length of database: 11,318,596
effective HSP length: 59
effective length of query: 24
effective length of database: 9,742,175
effective search space: 233812200
effective search space used: 233812200
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 52 (24.6 bits)
Lotus: description of TM0136.17