BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0134.16
(212 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g04970 unknown protein 195 1e-50
At3g20270 putative bactericidal permeability-increasing precurso... 170 4e-43
At1g50310 hexose transporter, putative 30 0.94
At4g35440 CLC-e chloride channel protein 28 2.7
At4g01920 putative CHP-rich zinc finger protein 28 3.6
At4g00560 putative dTDP-6-deoxy-L-mannose-dehydrogenase 28 4.6
At2g28540 unknown protein 28 4.6
At5g37070 putative protein 27 6.1
At5g05930 unknown protein 27 6.1
At4g01760 putative CHP-rich zinc finger protein 27 6.1
At1g02980 27 6.1
At3g08890 unknown protein 27 7.9
>At1g04970 unknown protein
Length = 488
Score = 195 bits (496), Expect = 1e-50
Identities = 97/200 (48%), Positives = 146/200 (72%), Gaps = 4/200 (2%)
Query: 1 VKGMQVGLTVNLKNQEGTLKLSLLDYGCYVGDLSIKLDGGTAWLYQLLVDAFGGNIASSV 60
V+GM++GL++ LK+ EG LKLSL + GC+V D++I+L+GG +W YQ +V+AF I SSV
Sbjct: 137 VQGMEIGLSLGLKSDEGGLKLSLSECGCHVEDITIELEGGASWFYQGMVNAFKDQIGSSV 196
Query: 61 EEAVSEKINEGIAKLDKFLQSLPKQIPLDKTSALNVSFVGNPVLSNSSIAIAINGLFT-- 118
E +++K+ EG++ LD FLQSLPK+IP+D + LNV+F +P+L NSSI I+GLFT
Sbjct: 197 ESTIAKKLTEGVSDLDSFLQSLPKEIPVDDNADLNVTFTSDPILRNSSITFEIDGLFTKG 256
Query: 119 ERSEVVESEGYKKGFKISSACGGLPNMIKVSLHEYVIQSASLVYFNAGKMQLIIDELPDQ 178
E ++V++S +KK + C G M+ +S+ E V SA+ +Y+NA +Q ++D++P+Q
Sbjct: 257 ETNQVLKS-FFKKSVSL-VICPGNSKMLGISVDEAVFNSAAALYYNADFVQWVVDKIPEQ 314
Query: 179 VILNTAECRFIVPQLYKQYP 198
+LNTA RFI+PQLYK+YP
Sbjct: 315 SLLNTARWRFIIPQLYKKYP 334
>At3g20270 putative bactericidal permeability-increasing precursor
protein
Length = 515
Score = 170 bits (431), Expect = 4e-43
Identities = 91/199 (45%), Positives = 133/199 (66%), Gaps = 5/199 (2%)
Query: 1 VKGMQVGLTVNLKNQEGTLKLSLLDYGCYVGDLSIKLDGGTAWLYQLLVDAFGGNIASSV 60
VKGM V +T L N G+LK++ + C V ++ I ++GG +WLYQ +VDAF I S+V
Sbjct: 173 VKGMNVRITATLVNDNGSLKIASRENDCTVKNIDIHINGGASWLYQGVVDAFQKMIISTV 232
Query: 61 EEAVSEKINEGIAKLDKFLQSLPKQIPLDKTSALNVSFVGNPVLSNSSIAIAINGLFTER 120
E+ VS KI E + KLD FLQSLPKQ +D ++A+N++F GNPVL NSS+ + INGLF +
Sbjct: 233 EKTVSTKIVEKMKKLDSFLQSLPKQRKIDDSAAVNLTFTGNPVLGNSSVEVDINGLFMPK 292
Query: 121 SEVVESEGYKKGFKISSACGGL-PNMIKVSLHEYVIQSASLVYFNAGKMQLIIDELPDQV 179
+ ++ G + SS GG+ M+ +S+ E V SA+LVYFNA M L+++E +
Sbjct: 293 GDDIKVAGSRS----SSFFGGVNKRMVTISVEEGVFNSATLVYFNAKVMHLVMEETKNGS 348
Query: 180 ILNTAECRFIVPQLYKQYP 198
IL+T++ + I+P+LYK YP
Sbjct: 349 ILSTSDWKLILPELYKHYP 367
>At1g50310 hexose transporter, putative
Length = 517
Score = 30.0 bits (66), Expect = 0.94
Identities = 28/110 (25%), Positives = 44/110 (39%), Gaps = 10/110 (9%)
Query: 39 GGTAWLYQLLVDAFGGNIASSVEEAVSEKINEGIAKLDKFLQSLPKQIPLDKTSALNVSF 98
GG A+L L +AF N+A + + + G A + L + P ALN+ F
Sbjct: 118 GGVAFLIGSLFNAFATNVAMLIVGRLLLGVGVGFANQSTPVY-LSEMAPAKIRGALNIGF 176
Query: 99 VGNPVLSNSSIAIAINGLFTERSEVVESEGYKKGFKISSACGGLPNMIKV 148
+AI I L S+ K G+++S +P +I V
Sbjct: 177 ---------QMAITIGILIANLINYGTSQMAKNGWRVSLGLAAVPAVIMV 217
>At4g35440 CLC-e chloride channel protein
Length = 710
Score = 28.5 bits (62), Expect = 2.7
Identities = 23/90 (25%), Positives = 42/90 (46%), Gaps = 12/90 (13%)
Query: 40 GTAWLYQLLVDAFGG---NIASSVEEAVSEKINEGIAKLDK-------FLQSLPKQIPLD 89
G+ WL +LV GG +I + + E+ + + + LD+ FL+++ + L
Sbjct: 123 GSNWLRVILVPTIGGLVVSILNQLRESAGKSTGDSHSSLDRVKAVLRPFLKTVAACVTLG 182
Query: 90 KTSALNVSFVGNPVLSNSSIAIAINGLFTE 119
++L G V +SIA +N LF +
Sbjct: 183 TGNSLGPE--GPSVEIGASIAKGVNSLFNK 210
>At4g01920 putative CHP-rich zinc finger protein
Length = 658
Score = 28.1 bits (61), Expect = 3.6
Identities = 15/47 (31%), Positives = 26/47 (54%), Gaps = 3/47 (6%)
Query: 115 GLFTERSEVVESEGYKKGFKISSACGGLPNMIKVSLHEYVIQSASLV 161
GL +RS V ++ GF I C GLP +I ++ H++ + S++
Sbjct: 264 GLKGDRSPYV---CFQCGFMIHQDCLGLPRLININRHDHRVSRTSVL 307
>At4g00560 putative dTDP-6-deoxy-L-mannose-dehydrogenase
Length = 267
Score = 27.7 bits (60), Expect = 4.6
Identities = 37/164 (22%), Positives = 66/164 (39%), Gaps = 29/164 (17%)
Query: 48 LVDAFGGNIASSVE-------EAVSEKINEGIAKLDKFLQSLPKQIPLDKTSALNV---- 96
L+DAF + A V+ ++S+ + ++ S+P+ D SA+++
Sbjct: 50 LLDAFPHSPAFPVDLKSGLGLNSISQDFRQPDVVVNCAALSVPRACEQDPDSAMSINVPT 109
Query: 97 -------SFVGNPVL----SNSSIAIAINGLFTERSEVVESEGYKKGFKISSACGGLPNM 145
SF N L S + + + E E V Y K K+++ +
Sbjct: 110 SLVNWLSSFETNKTLLIHLSTDQVYQGVKSFYKEEDETVAVNVYGKS-KVAAEL-----L 163
Query: 146 IKVSLHEYVIQSASLVYFNAGKMQLIIDELPDQVILNTAECRFI 189
IK + I +S++ F + + LP QV+LNT R+I
Sbjct: 164 IKDKCQSFAILRSSII-FGPQTVSPLPKTLPIQVLLNTISQRYI 206
>At2g28540 unknown protein
Length = 652
Score = 27.7 bits (60), Expect = 4.6
Identities = 25/92 (27%), Positives = 40/92 (43%), Gaps = 3/92 (3%)
Query: 57 ASSVEEAVSEKINEGIAKLDKFLQSLPKQIPLDKTSALNVSFVGNPVLSNSSIAIAIN-G 115
A S++ A S K+ + ++ Q PK D +A N GN NSS + N G
Sbjct: 363 ARSLKLASSSKVEDNQSRFSFARQEEPKDQAFDSYNASNQMSRGNDFYQNSSERQSPNMG 422
Query: 116 LFTERSEVVESEGYKKGFKISSACGGLPNMIK 147
+F + + S Y++G + LP+ K
Sbjct: 423 MFGTYNGL--SSCYRRGLDYVTESSTLPSSYK 452
>At5g37070 putative protein
Length = 170
Score = 27.3 bits (59), Expect = 6.1
Identities = 37/152 (24%), Positives = 57/152 (37%), Gaps = 42/152 (27%)
Query: 30 VGD----LSIKLDGGTAWLYQL-----------LVDAFGGNIASSVEEAVSEKINEGIAK 74
VGD LS ++GGT WL L+ FG + ++A + + NE K
Sbjct: 24 VGDDFNSLSSSIEGGTKWLVNKLKGKMQKPLPELLKEFGLPVGIFPQDATNYEFNEETGK 83
Query: 75 LDKFLQSLPKQIPLDKTSALNVSFVGNPVLSNSSIAIAINGLFTERSEVVESEGYKKGFK 134
L F+ V + + VL S+ + E+ ++ E EG K
Sbjct: 84 LTVFI-----------PETCEVGYRDSSVLRFSTTVTG----YLEKGKLAEVEGMKTKVM 128
Query: 135 ISSACGGLPNMIKVSLHEYVIQSASLVYFNAG 166
I +KV+ + +S VYF AG
Sbjct: 129 I---------WVKVTC---ISADSSKVYFTAG 148
>At5g05930 unknown protein
Length = 274
Score = 27.3 bits (59), Expect = 6.1
Identities = 15/62 (24%), Positives = 33/62 (53%)
Query: 52 FGGNIASSVEEAVSEKINEGIAKLDKFLQSLPKQIPLDKTSALNVSFVGNPVLSNSSIAI 111
FG N S+EE E++ E + ++D + + + + ++++ + +LS + IAI
Sbjct: 124 FGANPNYSIEEFYKEQLPEDLVRVDLLFRKAHESGIIIQCRSVSIHEISCLLLSGNYIAI 183
Query: 112 AI 113
A+
Sbjct: 184 AL 185
>At4g01760 putative CHP-rich zinc finger protein
Length = 667
Score = 27.3 bits (59), Expect = 6.1
Identities = 11/29 (37%), Positives = 17/29 (57%)
Query: 133 FKISSACGGLPNMIKVSLHEYVIQSASLV 161
F I C GLP +I ++ H++ I SL+
Sbjct: 285 FLIHKDCFGLPRLININRHDHRISRTSLI 313
>At1g02980
Length = 648
Score = 27.3 bits (59), Expect = 6.1
Identities = 13/38 (34%), Positives = 20/38 (52%)
Query: 59 SVEEAVSEKINEGIAKLDKFLQSLPKQIPLDKTSALNV 96
SV EA + G+AKL K L+ +P + P D + +
Sbjct: 6 SVLEAGWSVMEAGVAKLQKILEEVPDEPPFDPVQRMQL 43
>At3g08890 unknown protein
Length = 170
Score = 26.9 bits (58), Expect = 7.9
Identities = 27/125 (21%), Positives = 47/125 (37%), Gaps = 24/125 (19%)
Query: 30 VGDLSIKLDGGTAWLYQL-----------LVDAFGGNIASSVEEAVSEKINEGIAKLDKF 78
+ LS ++GGT WL L+ FG + +A + + NE KL F
Sbjct: 28 INSLSSSIEGGTKWLVNKIKGKMQKPLPELLKEFGLPVGIFPRDATNYEFNEQTRKLTVF 87
Query: 79 LQSLPKQIPLDKTSALNVSFVGNPVLSNSSIA------------IAINGLFTERSEVVES 126
+ S+ ++ TS L + L +A + + + + S+V +
Sbjct: 88 IPSI-CEVGYKDTSVLRFTTTVTGFLEKGKLADVEGMKTKVMIWVKVTSISADSSKVHFT 146
Query: 127 EGYKK 131
G KK
Sbjct: 147 AGMKK 151
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.319 0.138 0.388
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,334,498
Number of Sequences: 26719
Number of extensions: 167569
Number of successful extensions: 536
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 526
Number of HSP's gapped (non-prelim): 15
length of query: 212
length of database: 11,318,596
effective HSP length: 95
effective length of query: 117
effective length of database: 8,780,291
effective search space: 1027294047
effective search space used: 1027294047
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0134.16