BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0132.5
(458 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g65720 NifS-like aminotranfserase 716 0.0
At1g08490 NIFS like protein CpNifsp precursor (NIFS) 112 3e-25
At1g27980 unknown protein 59 7e-09
At1g16540 molybdenum cofactor sulfurase 42 0.001
At3g62130 unknown protein 36 0.051
At1g08630 unknown protein 36 0.051
At3g60610 putative protein 32 0.56
At2g17580 poly(A) polymerase like protein 30 2.1
At1g79030 hypothetical protein 30 2.8
At3g22428 hypothetical protein 29 6.2
>At5g65720 NifS-like aminotranfserase
Length = 453
Score = 716 bits (1849), Expect = 0.0
Identities = 358/459 (77%), Positives = 404/459 (87%), Gaps = 7/459 (1%)
Query: 1 MTSKLLASTLRHHLTKPTQLLRR-HFLSTAAAAAAAAEPQQHHDESAGLTMKGVKIAGRP 59
M SK++++T+R LTKP R +LSTAAAA + DES + MKGV+I+GRP
Sbjct: 1 MASKVISATIRRTLTKPHGTFSRCRYLSTAAAATEV----NYEDES--IMMKGVRISGRP 54
Query: 60 LYLDVQATSPVDPRVLDAMLPFYISRYGNPHSRTHFYGWESDEAVENARAQVASLIGASP 119
LYLD+QAT+P+DPRV DAM I YGNPHSRTH YGWE++ AVENAR QVA LI ASP
Sbjct: 55 LYLDMQATTPIDPRVFDAMNASQIHEYGNPHSRTHLYGWEAENAVENARNQVAKLIEASP 114
Query: 120 KEIVFTSGATESNNISIKGVLHFYKEKKRHVITTQTEHKCVLDSCRHLQQEGFDVTYLPV 179
KEIVF SGATE+NN+++KGV+HFYK+ K+HVITTQTEHKCVLDSCRHLQQEGF+VTYLPV
Sbjct: 115 KEIVFVSGATEANNMAVKGVMHFYKDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYLPV 174
Query: 180 ESDGLVDLEKLRAAIRPDTGLVSVMAVNNEIGVFQPMEEIGRICKEFNVPFHTDAAQALG 239
++DGLVDLE LR AIRPDTGLVS+MAVNNEIGV QPMEEIG ICKE NVPFHTDAAQA+G
Sbjct: 175 KTDGLVDLEMLREAIRPDTGLVSIMAVNNEIGVVQPMEEIGMICKEHNVPFHTDAAQAIG 234
Query: 240 KVPVDVEKWNVSLMSLSGHKIYGPKGVGALYLRRRPRIRVEPQMNGGGQERGIRSGTIPT 299
K+PVDV+KWNV+LMS+S HKIYGPKGVGALY+RRRPRIR+EP MNGGGQERG+RSGT T
Sbjct: 235 KIPVDVKKWNVALMSMSAHKIYGPKGVGALYVRRRPRIRLEPLMNGGGQERGLRSGTGAT 294
Query: 300 PLVVGMGAACEVAMKEMEYDEKRISALQERLLNGIREKLDGVVVNGSMERRYAGNLNLSF 359
+VG GAACE+AMKEMEYDEK I LQERLLNG+REKLDGVVVNGSM+ RY GNLNLSF
Sbjct: 295 QQIVGFGAACELAMKEMEYDEKWIKGLQERLLNGVREKLDGVVVNGSMDSRYVGNLNLSF 354
Query: 360 AYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVEEDMAHTSIRFGIGRFTTEAE 419
AYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGV+EDMAHTSIRFGIGRFTT+ E
Sbjct: 355 AYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTKEE 414
Query: 420 IDRAIQLTVHQVEKLREMSPLYEMVKEGINIKDIQWAQH 458
ID+A++LTV QVEKLREMSPLYEMVKEGI+IK+IQW+QH
Sbjct: 415 IDKAVELTVKQVEKLREMSPLYEMVKEGIDIKNIQWSQH 453
>At1g08490 NIFS like protein CpNifsp precursor (NIFS)
Length = 463
Score = 112 bits (281), Expect = 3e-25
Identities = 87/274 (31%), Positives = 130/274 (46%), Gaps = 12/274 (4%)
Query: 26 LSTAAAAAAAAEPQQHHDESAGL---TMKGVKI------AGRPLYLDVQATSPVDPRVLD 76
LS +AAAA++ ES L K +I + +YLD ATS VLD
Sbjct: 31 LSVCSAAAASSATISTDSESVSLGHRVRKDFRILHQEVNGSKLVYLDSAATSQKPAAVLD 90
Query: 77 AMLPFYISRYGNPHSRTHFYGWESDEAVENARAQVASLIGASP-KEIVFTSGATESNN-I 134
A+ +Y N H H+ ++ + E AR +VA I AS +EIVFT ATE+ N +
Sbjct: 91 ALQNYYEFYNSNVHRGIHYLSAKATDEFELARKKVARFINASDSREIVFTRNATEAINLV 150
Query: 135 SIKGVLHFYKEKKRHVITTQTEHKCVLDSCRHLQQEGFDVTYLPVESDGLVDLEKLRAAI 194
+ L K ++T H C++ Q+ G + ++ + D + D+ KLR I
Sbjct: 151 AYSWGLSNLKPGDEVILTVAEHHSCIVPWQIVSQKTGAVLKFVTLNEDEVPDINKLRELI 210
Query: 195 RPDTGLVSVMAVNNEIGVFQPMEEIGRICKEFNVPFHTDAAQALGKVPVDVEKWNVSLMS 254
P T LV+V V+N + P+EEI + DA Q++ + VDV+K N +
Sbjct: 211 SPKTKLVAVHHVSNVLASSLPIEEIVVWAHDVGAKVLVDACQSVPHMVVDVQKLNADFLV 270
Query: 255 LSGHKIYGPKGVGALYLRRRPRIRVEPQMNGGGQ 288
S HK+ GP G+G LY + + P GGG+
Sbjct: 271 ASSHKMCGPTGIGFLY-GKSDLLHSMPPFLGGGE 303
>At1g27980 unknown protein
Length = 544
Score = 58.5 bits (140), Expect = 7e-09
Identities = 69/257 (26%), Positives = 116/257 (44%), Gaps = 34/257 (13%)
Query: 109 AQVASLIG----ASPKEIV--FTSGATESNNISIKGVLHFYKEKKR----HVITTQTEHK 158
A A+L+G AS +I TSG TES +++K + K KK +I ++ H
Sbjct: 181 AMTAALLGSKETASGGQICGNMTSGGTESIVLAVKSSRDYMKYKKGITRPEMIIPESGHS 240
Query: 159 CVLDSCRHLQQEGFDVTYLPVESDGLVDLEKLRAAIRPDTGLVSVMAVNNEIGVFQPMEE 218
+ ++ + + + V PV+ D D++ R I +T ++ A G+ P+EE
Sbjct: 241 AYDKAAQYFKIKLWRV---PVDKDFRADVKATRRHINRNTIMIVGSAPGFPHGIIDPIEE 297
Query: 219 IGRICKEFNVPFHTDA---------AQALGK--VPVDVEKWNVSLMSLSGHKIYG--PKG 265
+G++ + + FH D A+ LG P D V+ +S+ HK YG PKG
Sbjct: 298 LGQLALSYGICFHVDLCLGGFVLPFARKLGYQIPPFDFSVQGVTSISVDVHK-YGLAPKG 356
Query: 266 VGALYLR----RRPRIRVEPQMNGGGQERGIRSGTIPTPLVVGMGAACEVAMKEMEY--D 319
+ R R+ + + +GG +G+ P LV G AA +++ E Y +
Sbjct: 357 TSTVLYRNHEIRKHQFVAVTEWSGGLYVSPTIAGSRPGSLVAGAWAAM-MSLGEEGYLQN 415
Query: 320 EKRISALQERLLNGIRE 336
+I +RL G+RE
Sbjct: 416 TSKIMEASKRLEEGVRE 432
>At1g16540 molybdenum cofactor sulfurase
Length = 819
Score = 41.6 bits (96), Expect = 0.001
Identities = 52/226 (23%), Positives = 90/226 (39%), Gaps = 43/226 (19%)
Query: 86 YGNPHSRTHFYGWESDEAVENARAQVASLIGASPKEI--VFTSGATESNNISIKGVLHFY 143
+GNPHS++ SD + +AR QV ASP++ +FTSGAT + + + G +
Sbjct: 64 FGNPHSQSDISSATSD-LIADARHQVLEYFNASPEDYSCLFTSGATAA--LKLVGETFPW 120
Query: 144 KEKKRHVITTQTEHKCVLDSCRHLQQEGFDVTYLPVE-----------SDGLVDLEKLRA 192
+ + T + H VL + +G + +E S + ++
Sbjct: 121 TQDSNFLYTMEN-HNSVLGIREYALAQGASACAVDIEEAANQPGQLTNSGPSIKVKHRAV 179
Query: 193 AIRPDTGLVSVMAVNNEIGVFQPMEEIGRICKEFNV-----------------PFHT--- 232
+R + L + N +F E FN+ PF
Sbjct: 180 QMRNTSKLQKEESRGNAYNLFAFPSECNFSGLRFNLDLVKLMKENTETVLQGSPFSKSKR 239
Query: 233 -----DAAQALGKVPVDVEKWNVSLMSLSGHKIYG-PKGVGALYLR 272
DAA+ +P D+ ++ + LS +K++G P G+GAL +R
Sbjct: 240 WMVLIDAAKGCATLPPDLSEYPADFVVLSFYKLFGYPTGLGALLVR 285
>At3g62130 unknown protein
Length = 454
Score = 35.8 bits (81), Expect = 0.051
Identities = 46/186 (24%), Positives = 76/186 (40%), Gaps = 19/186 (10%)
Query: 107 ARAQVASLIGASP-KEIVFTSGATESNNISIKGVLHFYKEKKRHVITTQTEHKCVLDSCR 165
+R ++ LI A E+ AT + I ++ V + E K T C S +
Sbjct: 89 SRTVISDLINADDVDEVSLVDNATTAAAIVLQKVGRCFSEGKYKKEDTVVMFHCAFQSVK 148
Query: 166 -----HLQQEGFDVTYL----PVESDGLV------DLEKLRAAIRPDTGLVSVMAVNNEI 210
++ + G + PV S+ + LEK RA R L + + +
Sbjct: 149 KSIQAYVSRVGGSTVEVRLPFPVNSNEEIISKFREGLEKGRANGRT-VRLAIIDHITSMP 207
Query: 211 GVFQPMEEIGRICKEFNVP-FHTDAAQALGKVPVDVEKWNVSLMSLSGHK-IYGPKGVGA 268
V P+ E+ +IC+E V DAA A+G V VDV++ + HK + P +
Sbjct: 208 CVLMPVRELVKICREEGVEQVFVDAAHAIGSVKVDVKEIGADYYVSNLHKWFFCPPSIAF 267
Query: 269 LYLRRR 274
Y ++R
Sbjct: 268 FYCKKR 273
>At1g08630 unknown protein
Length = 358
Score = 35.8 bits (81), Expect = 0.051
Identities = 47/188 (25%), Positives = 75/188 (39%), Gaps = 32/188 (17%)
Query: 180 ESDGLVDLEKLRAAIR--------PDTGLVSVMAVNNEIG----VFQPMEEIGRICKEFN 227
E DG +DLE + AAIR P T L+ + + G + E++G I K
Sbjct: 111 EEDGTMDLEAIEAAIRDPKGSTFYPSTRLICLENTHANSGGRCLSVEYTEKVGEIAKRHG 170
Query: 228 VPFHTDAAQ------ALGKVPVDVEKWNVSLMSLSGHKIYGPKG---VGALYLRRRPRIR 278
V H D A+ ALG + K S+ + P G VG+ + +
Sbjct: 171 VKLHIDGARLFNASIALGVPVHKLVKAADSVQVCLSKGLGAPVGSVIVGSQSFIEKAK-T 229
Query: 279 VEPQMNGGGQERGIRSGTIPTPLVVGMGAACEVAMKEMEYDEKRISALQERL--LNGIRE 336
V + GG ++ G+ L A + + ++++D K+ L E L + GIR
Sbjct: 230 VRKTLGGGMRQIGV--------LCAAALVALQENLPKLQHDHKKAKLLAEGLNQMKGIRV 281
Query: 337 KLDGVVVN 344
+ V N
Sbjct: 282 NVAAVETN 289
>At3g60610 putative protein
Length = 442
Score = 32.3 bits (72), Expect = 0.56
Identities = 23/76 (30%), Positives = 38/76 (49%), Gaps = 13/76 (17%)
Query: 322 RISALQERLLNGIREKLDGVVVNGSMERRYAGNL-------NLSFAYVEGESLLMGLKEV 374
R+S++Q +L K++ V ER+YAG +L+F ++G L + +
Sbjct: 371 RVSSVQSKL------KINAKVAKKLKERQYAGGATTSGLTSSLAFTSMQGIELCNPQQAL 424
Query: 375 AVSSGSACTSASLEPS 390
+ SG+ TS S EPS
Sbjct: 425 GLGSGAQSTSQSQEPS 440
>At2g17580 poly(A) polymerase like protein
Length = 757
Score = 30.4 bits (67), Expect = 2.1
Identities = 20/64 (31%), Positives = 29/64 (45%), Gaps = 7/64 (10%)
Query: 64 VQATSPVDPRVLDAMLPFYISRYGNPHS-------RTHFYGWESDEAVENARAQVASLIG 116
V P DP++ A+L F+I+ NP Y +AVE AR S+IG
Sbjct: 352 VSCDQPADPKLWIAVLAFHIALVRNPQEAIVVRAFAALLYHGNWSKAVEFAREHETSVIG 411
Query: 117 ASPK 120
+P+
Sbjct: 412 YAPE 415
>At1g79030 hypothetical protein
Length = 505
Score = 30.0 bits (66), Expect = 2.8
Identities = 17/62 (27%), Positives = 32/62 (51%), Gaps = 4/62 (6%)
Query: 360 AYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVEEDMAHTSIRFGIGRFTTEAE 419
A+V ES + + E A+ G AC + PS+ + +G+E+ T+ R RF + +
Sbjct: 397 AFVCAESKIFDVSEWAICQGMACRPNTHRPSFHVNMVGLEK----TTQRSNSSRFPWDLD 452
Query: 420 ID 421
++
Sbjct: 453 VE 454
>At3g22428 hypothetical protein
Length = 342
Score = 28.9 bits (63), Expect = 6.2
Identities = 21/95 (22%), Positives = 35/95 (36%), Gaps = 4/95 (4%)
Query: 59 PLYLDVQATSPVDPRVLDAMLPFYISRYGNPHSRTHFYGWES----DEAVENARAQVASL 114
P Y+D S + V PFY + + + + G E DE E R + +
Sbjct: 147 PNYMDFPPQSRLAGPVFGRFPPFYPEEFRDQRMKRKYPGEEEIDRRDERAEMMRQRQQFM 206
Query: 115 IGASPKEIVFTSGATESNNISIKGVLHFYKEKKRH 149
A+P + F +G + ++ H RH
Sbjct: 207 QYANPNDHSFMAGTSRDVGEDVRAAKHMRVGSSRH 241
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.318 0.134 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,918,925
Number of Sequences: 26719
Number of extensions: 424458
Number of successful extensions: 1036
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 1029
Number of HSP's gapped (non-prelim): 10
length of query: 458
length of database: 11,318,596
effective HSP length: 103
effective length of query: 355
effective length of database: 8,566,539
effective search space: 3041121345
effective search space used: 3041121345
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0132.5