BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0125.16
(229 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g23130 arabinogalactan-protein AGP17 36 0.019
At4g34440 serine/threonine protein kinase - like 35 0.033
At3g02540 RAD23 like protein 35 0.043
At2g28440 En/Spm-like transposon protein 35 0.043
At3g60280 uclacyanin 3 33 0.13
At5g58620 unknown protein 32 0.21
At4g02960 putative polyprotein of LTR transposon 32 0.21
At1g44020 hypothetical protein 32 0.21
At5g56890 unknown protein 32 0.28
At5g66540 unknown protein 32 0.36
At2g14410 hypothetical protein 31 0.48
At1g12080 unknown protein 31 0.48
At4g00390 unknown protein 31 0.62
At2g31270 unknown protein 31 0.62
At2g27090 unknown protein 31 0.62
At1g71710 RIBOSOMAL PROTEIN, putative (At1g71710) 31 0.62
At1g62760 hypothetical protein 31 0.62
At1g20290 hypothetical protein 31 0.62
At5g05990 unknown protein 30 0.81
At4g03920 putative protein 30 0.81
>At2g23130 arabinogalactan-protein AGP17
Length = 185
Score = 35.8 bits (81), Expect = 0.019
Identities = 19/62 (30%), Positives = 30/62 (47%), Gaps = 2/62 (3%)
Query: 88 PGTGRGPPSPPSSSSDEEDPSEIEADVDAPVAPLPATAVDLTDVASSSRLVEPKREPVVP 147
P P+P S+ + + P +EA V+AP +P PA+ ++ A S P + P
Sbjct: 44 PAISPAAPTPESTEAPAKTP--VEAPVEAPPSPTPASTPQISPPAPSPEADTPSAPEIAP 101
Query: 148 SA 149
SA
Sbjct: 102 SA 103
Score = 29.6 bits (65), Expect = 1.4
Identities = 15/55 (27%), Positives = 25/55 (45%)
Query: 94 PPSPPSSSSDEEDPSEIEADVDAPVAPLPATAVDLTDVASSSRLVEPKREPVVPS 148
PPSP +S+ + P + D P AP A + D+ A + + K+ P+
Sbjct: 71 PPSPTPASTPQISPPAPSPEADTPSAPEIAPSADVPAPALTKHKKKTKKHKTAPA 125
>At4g34440 serine/threonine protein kinase - like
Length = 670
Score = 35.0 bits (79), Expect = 0.033
Identities = 27/76 (35%), Positives = 29/76 (37%), Gaps = 4/76 (5%)
Query: 75 ELSFNLPPSVRCGPGTGRGPPSPPSS---SSDEEDPSEIEADVDAPVAPLPATAVDLTDV 131
E S PPS P PP+PPSS SS P +I A P AP P
Sbjct: 13 ETSNGTPPSNGTSPSNESSPPTPPSSPPPSSISAPPPDISASFSPPPAP-PTQETSPPTS 71
Query: 132 ASSSRLVEPKREPVVP 147
SSS V P P
Sbjct: 72 PSSSPPVVANPSPQTP 87
>At3g02540 RAD23 like protein
Length = 419
Score = 34.7 bits (78), Expect = 0.043
Identities = 33/118 (27%), Positives = 52/118 (43%), Gaps = 14/118 (11%)
Query: 51 YRGRVMEDRTD-ERGRLTRGVLRRMELSFNLPPSVRCGP---GTGRGPPSPPSSSSDEED 106
++G+V++D T E ++ + ++ + P S GT + PPS+S
Sbjct: 48 HQGKVLKDETTIEENKVAENSFIVIMMNKSKPASAAASSASAGTSQAKSIPPSTSQPSIS 107
Query: 107 PSEIEADVDAPVAPL------PATAVDLTDVASSSRLVEPKREPVVPSASRRFSFTPS 158
P + A V APVAP PA VA++ + P EPV + S S TP+
Sbjct: 108 P-QTPASVSAPVAPAPTRPPPPAPTPTPAPVAATETVTTPIPEPVPATIS---SSTPA 161
>At2g28440 En/Spm-like transposon protein
Length = 268
Score = 34.7 bits (78), Expect = 0.043
Identities = 22/58 (37%), Positives = 31/58 (52%), Gaps = 5/58 (8%)
Query: 95 PSPPSSSSDEEDP--SEIEADVDAPVAPLPATAVDLTDVASSSRLVEPKREPVVPSAS 150
P PPSSS +E+ P +VD+P+AP + VD SSS + P+ PS+S
Sbjct: 68 PLPPSSSPEEDSPLAPSSSPEVDSPLAPSSSPEVDSPQPPSSSPEAD---SPLPPSSS 122
Score = 28.9 bits (63), Expect = 2.4
Identities = 18/52 (34%), Positives = 26/52 (49%), Gaps = 2/52 (3%)
Query: 95 PSPPSSSSDEEDP--SEIEADVDAPVAPLPATAVDLTDVASSSRLVEPKREP 144
P PSSS +E+ P + D+P+AP + VD SSS V+ + P
Sbjct: 56 PLSPSSSPEEDSPLPPSSSPEEDSPLAPSSSPEVDSPLAPSSSPEVDSPQPP 107
Score = 28.1 bits (61), Expect = 4.0
Identities = 16/55 (29%), Positives = 25/55 (45%)
Query: 95 PSPPSSSSDEEDPSEIEADVDAPVAPLPATAVDLTDVASSSRLVEPKREPVVPSA 149
P PPSSS + + P + +A PA++ +A S P +P PS+
Sbjct: 104 PQPPSSSPEADSPLPPSSSPEANSPQSPASSPKPESLADSPSPPPPPPQPESPSS 158
>At3g60280 uclacyanin 3
Length = 222
Score = 33.1 bits (74), Expect = 0.13
Identities = 23/70 (32%), Positives = 29/70 (40%), Gaps = 4/70 (5%)
Query: 81 PPSVRCGPGTGRGPPSPPSSSSDEEDPSEIEADVDAPVAPLPATAVDLTDVASSSRLVEP 140
PPS P T PPS PS+ S P P + LP +A T+ S + P
Sbjct: 131 PPST---PSTPSSPPSTPSTPSSPPSPPS-PPSPSLPPSSLPPSASPPTNGTPDSETLTP 186
Query: 141 KREPVVPSAS 150
P+ PS S
Sbjct: 187 PPAPLPPSLS 196
>At5g58620 unknown protein
Length = 607
Score = 32.3 bits (72), Expect = 0.21
Identities = 26/80 (32%), Positives = 36/80 (44%), Gaps = 12/80 (15%)
Query: 47 HRATYRGRVMEDRTDERGRLTRGVLRRMELSFNLPPSVR-CGPGTGRGPPSPPSSSSDEE 105
H A YR R+ +D T+ RR+ + P +R P TG G PSP SS S
Sbjct: 281 HPAQYRTRLCKDETN--------CSRRVCFFAHKPEELRPLYPSTGSGVPSPRSSFSSCN 332
Query: 106 DPSEIEADVDAPVAPLPATA 125
+ + P++PLP A
Sbjct: 333 SSTAFDM---GPISPLPIGA 349
>At4g02960 putative polyprotein of LTR transposon
Length = 1456
Score = 32.3 bits (72), Expect = 0.21
Identities = 15/54 (27%), Positives = 29/54 (52%)
Query: 95 PSPPSSSSDEEDPSEIEADVDAPVAPLPATAVDLTDVASSSRLVEPKREPVVPS 148
P+ PS +S ++ ++ + +P P P+T++ + SSS P PV+P+
Sbjct: 842 PNSPSPNSPNQNSPLPQSPISSPHIPTPSTSISEPNSPSSSSTSTPPLPPVLPA 895
>At1g44020 hypothetical protein
Length = 654
Score = 32.3 bits (72), Expect = 0.21
Identities = 17/33 (51%), Positives = 25/33 (75%), Gaps = 2/33 (6%)
Query: 199 DLDTEMITVDS--DSDTDVDNYSPSDEEEEEEL 229
++D+E++ V S DSD+D D+ S+EEEEEEL
Sbjct: 143 EVDSELMFVHSSMDSDSDSDSDLDSEEEEEEEL 175
>At5g56890 unknown protein
Length = 1113
Score = 32.0 bits (71), Expect = 0.28
Identities = 23/77 (29%), Positives = 36/77 (45%), Gaps = 11/77 (14%)
Query: 88 PGTGRGPPS----PPSSSSDEEDPSEIEADVDAPVAPLPATAVDLTDVASSSRLVEPKRE 143
P PP+ PP ++S+ P IE + APVA P ++D+ V P
Sbjct: 165 PSVALPPPTPSNVPPRNASNNHKPPPIEKSI-APVASPPTISIDIAPP------VHPVIP 217
Query: 144 PVVPSASRRFSFTPSRG 160
+ PS+S + TP++G
Sbjct: 218 KLTPSSSPVPTSTPTKG 234
>At5g66540 unknown protein
Length = 524
Score = 31.6 bits (70), Expect = 0.36
Identities = 32/140 (22%), Positives = 65/140 (45%), Gaps = 16/140 (11%)
Query: 96 SPPSSSSDEEDPSE---IEADVDAPVAPLPATAVDLTDVASSSRLVEPKREPVVPSAS-- 150
+P S S D S+ ++ P P + D D + ++ + +P++ S
Sbjct: 25 APSSISEDARSASQYLFMKLKPHNPKCPFDQLSSDGFDAEQIWQQIDMQSQPLLTSLRQE 84
Query: 151 -RRFSFTPSRGYRV-ELLVRVVEEDVLSEEVDVEMGSTRVVRRIVRKEVMDLDTEMITVD 208
+RF+ P ++ +L ++V ED + +E+D++ + +V D D E+ + D
Sbjct: 85 VKRFAKNPEEIRKLGKLALKVSHEDDI-DEMDMDGFDS--------DDVDDEDKEIESND 135
Query: 209 SDSDTDVDNYSPSDEEEEEE 228
S+ + + + +EEEEEE
Sbjct: 136 SEGEDEEEEEEDEEEEEEEE 155
>At2g14410 hypothetical protein
Length = 679
Score = 31.2 bits (69), Expect = 0.48
Identities = 33/172 (19%), Positives = 66/172 (38%), Gaps = 24/172 (13%)
Query: 77 SFNLPPSVRCGPGTGRGPPSPPSSSSDEEDPSEI-EADVDAPVAPLPATAVDLTDVASSS 135
S PP V P +G G S+ D +E+ +A A + P + + +
Sbjct: 330 SMGPPPPVTVSPSSGSGGEKAERSAIPRRDETEVRDAGTKAVLVPQTKSLTAMETERNVF 389
Query: 136 RLVEPKREPVVPSASR-------RFSFTPSRGYRV-----------ELLVRVVEEDVL-- 175
R + +++ ++P+ R RF R E L+ E+DV+
Sbjct: 390 RCLRTRKDTILPTFDRWRPAIRERFLLPAHHSSRANSELNDMIEYYERLLLDREKDVMAW 449
Query: 176 ---SEEVDVEMGSTRVVRRIVRKEVMDLDTEMITVDSDSDTDVDNYSPSDEE 224
S ++ ++ S++ R + ++ +L TE++ + + Y EE
Sbjct: 450 KDKSSSLESDLHSSKDARLKLEDQLDNLSTELMKSNGELQEQYQRYDKIQEE 501
>At1g12080 unknown protein
Length = 104
Score = 31.2 bits (69), Expect = 0.48
Identities = 17/49 (34%), Positives = 27/49 (54%)
Query: 180 DVEMGSTRVVRRIVRKEVMDLDTEMITVDSDSDTDVDNYSPSDEEEEEE 228
+VE+ + V +V E D +TE T + D T+V +P EEEE++
Sbjct: 16 NVEVPTKTVEETVVETEKKDEETEKKTEEKDEKTEVITETPVVEEEEKK 64
>At4g00390 unknown protein
Length = 364
Score = 30.8 bits (68), Expect = 0.62
Identities = 17/60 (28%), Positives = 30/60 (49%), Gaps = 3/60 (5%)
Query: 94 PPSPPSSSSDEEDPSEIE---ADVDAPVAPLPATAVDLTDVASSSRLVEPKREPVVPSAS 150
PP+ PSS D+ + SE + ++ D P+ LPAT +++ P + V +A+
Sbjct: 7 PPTAPSSDEDDVETSEDDSSSSEEDEPIKSLPATTAAAPAKSTAVSAATPAKSTSVSAAA 66
>At2g31270 unknown protein
Length = 571
Score = 30.8 bits (68), Expect = 0.62
Identities = 29/138 (21%), Positives = 59/138 (42%), Gaps = 11/138 (7%)
Query: 95 PSPPSSSSDEEDPSEIEADVDAPVAPLPATAVDLTDVASSSRLVEPKREPVVPSASRRFS 154
P+ P+S+ ++ SEI + PV P+ L S+ +++ PV+ + F+
Sbjct: 306 PTKPTSTPAKQTLSEINI-LPTPVKPVST----LAKFPSTPAIIDST--PVITATPPEFA 358
Query: 155 FTPSRGYRVELLVRVVEED---VLSEEVDVEMGSTRVVRRIVRKEVMDLDTEMITVDSDS 211
TP+R L R ++ +++ + T++VRR + + T+D
Sbjct: 359 STPARLMSTSLAARPLKRSNGHTNPDDISADP-PTKLVRRSLSLNFDSYPEDERTMDFTD 417
Query: 212 DTDVDNYSPSDEEEEEEL 229
D +D D ++E+
Sbjct: 418 DIPIDQVPEEDVSSDDEI 435
>At2g27090 unknown protein
Length = 743
Score = 30.8 bits (68), Expect = 0.62
Identities = 35/156 (22%), Positives = 59/156 (37%), Gaps = 15/156 (9%)
Query: 76 LSFNLPPSVRCGPGTGRGPPSPPSSSSDEEDPSEIEADVDAPVAPLPATAVDLTDVASS- 134
LS++ PP+ T PPSPPS+S + + + + V P ++ T +SS
Sbjct: 88 LSYSPPPASHSHHDTYSPPPSPPSTSPFQVNHMKFKGFSSKKVEEKPPVSIIATVTSSSI 147
Query: 135 --SRLVEPKREPVVPSASRRFSFTPSRGYRVELLVRVVEEDVLSEEVDVEMGSTRVVRRI 192
SR +E +S S P + + LS +D + S+ V
Sbjct: 148 PPSRSIEKMESTPFEESS---SMPPEAPW---------DYFGLSHPIDNQFSSSHVGNGH 195
Query: 193 VRKEVMDLDTEMITVDSDSDTDVDNYSPSDEEEEEE 228
V + V + D D D S + +++E
Sbjct: 196 VSRSVKEEDGTPEEEDDGEDFSFQEREESRDSDDDE 231
>At1g71710 RIBOSOMAL PROTEIN, putative (At1g71710)
Length = 664
Score = 30.8 bits (68), Expect = 0.62
Identities = 16/34 (47%), Positives = 22/34 (64%), Gaps = 1/34 (2%)
Query: 195 KEVMDLDTEMITVDSDSDTDVDNYSPSDEEEEEE 228
+E D+ E + +SDSD V+ + P DEEEEEE
Sbjct: 222 EETHDIIEEEVAFESDSDAGVEIH-PIDEEEEEE 254
>At1g62760 hypothetical protein
Length = 312
Score = 30.8 bits (68), Expect = 0.62
Identities = 17/56 (30%), Positives = 25/56 (44%)
Query: 95 PSPPSSSSDEEDPSEIEADVDAPVAPLPATAVDLTDVASSSRLVEPKREPVVPSAS 150
PSPPSSS PS + P++ P++ +S + P P PS+S
Sbjct: 38 PSPPSSSPSSAPPSSLSPSSPPPLSLSPSSPPPPPPSSSPLSSLSPSLSPSPPSSS 93
Score = 27.7 bits (60), Expect = 5.3
Identities = 19/65 (29%), Positives = 28/65 (42%), Gaps = 10/65 (15%)
Query: 81 PPSVRCGPGTGRGP---PSPPSSSSDEEDPSEIEADVDAPVA-------PLPATAVDLTD 130
PP P + P PSPPSSS PS + P++ P P ++ L+
Sbjct: 70 PPPPSSSPLSSLSPSLSPSPPSSSPSSAPPSSLSPSSPPPLSLSPSSPPPPPPSSSPLSS 129
Query: 131 VASSS 135
++ SS
Sbjct: 130 LSPSS 134
>At1g20290 hypothetical protein
Length = 497
Score = 30.8 bits (68), Expect = 0.62
Identities = 26/103 (25%), Positives = 45/103 (43%)
Query: 126 VDLTDVASSSRLVEPKREPVVPSASRRFSFTPSRGYRVELLVRVVEEDVLSEEVDVEMGS 185
V + D+ ++S V + V PS S + FTPS + E + EE+ EE + E
Sbjct: 319 VHVYDMEANSWRVLCSTKWVNPSDSDFYKFTPSMVHVGEGTIVQAEEEEEEEEEEEEEEE 378
Query: 186 TRVVRRIVRKEVMDLDTEMITVDSDSDTDVDNYSPSDEEEEEE 228
+E + + E + + + + + +EEEEEE
Sbjct: 379 EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE 421
>At5g05990 unknown protein
Length = 259
Score = 30.4 bits (67), Expect = 0.81
Identities = 27/113 (23%), Positives = 51/113 (44%), Gaps = 25/113 (22%)
Query: 141 KREPVVPSASRRFSFTPS---RGYRVELLVRVVEEDV-LSEEVD-------------VEM 183
+R + PS R FT S R + L+R++EE++ +E+ D EM
Sbjct: 34 RRRHLTPSVDRNVPFTTSTKKRASPTDPLLRIIEEEIGYAEKADDYHRVEETPSGFPFEM 93
Query: 184 -----GSTRVVRRIVRKEVMDLD---TEMITVDSDSDTDVDNYSPSDEEEEEE 228
G + R E + ++ T ++T D + D D + + ++E+E+E+
Sbjct: 94 EDKPGGKIVTLTREYEGETVKVEVHMTNLVTGDKEDDEDDEEEAENEEDEDED 146
>At4g03920 putative protein
Length = 463
Score = 30.4 bits (67), Expect = 0.81
Identities = 27/90 (30%), Positives = 37/90 (41%), Gaps = 12/90 (13%)
Query: 71 LRRMELSFNLPPSVRCGPGTGRGPPSPPSSSSDEEDPSEIEADVDAPVAPLPATAVDLTD 130
+ +++ S LP + P T PSPP S + + DV + V P+PA
Sbjct: 36 ISQIQPSATLPDAQAAVPQTALAIPSPPVPRSGSDLETLPIIDVTSAVTPVPAG------ 89
Query: 131 VASSSRLVEPKREPVVPSASRRFSFTPSRG 160
A S+ P P VP TPSRG
Sbjct: 90 -APSAATSVPAVAPSVPC-----EVTPSRG 113
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.315 0.133 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,637,631
Number of Sequences: 26719
Number of extensions: 267982
Number of successful extensions: 1641
Number of sequences better than 10.0: 97
Number of HSP's better than 10.0 without gapping: 38
Number of HSP's successfully gapped in prelim test: 61
Number of HSP's that attempted gapping in prelim test: 1458
Number of HSP's gapped (non-prelim): 199
length of query: 229
length of database: 11,318,596
effective HSP length: 96
effective length of query: 133
effective length of database: 8,753,572
effective search space: 1164225076
effective search space used: 1164225076
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0125.16