BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0119.3
(209 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g13600 hypothetical protein 35 0.022
At5g57200 putative protein 33 0.14
At5g05750 DnaJ-like protein 32 0.18
At3g57980 unknown protein 31 0.41
At3g28770 hypothetical protein 31 0.41
At2g27280 unknown protein 31 0.41
At2g21800 hypothetical protein 31 0.41
At3g50630 unknown protein 31 0.53
At2g36420 unknown protein 31 0.53
At4g26630 unknown protein 30 0.70
At3g53640 protein kinase -like protein 30 0.70
At1g10890 unknown protein 30 0.70
At1g03050 putative protein destination factor 30 0.70
At5g61330 unknown protein 30 0.91
At3g22860 eukaryotic translation initiation factor 3 (subunit 8) 30 0.91
At2g14470 pseudogene 30 0.91
At5g55660 putative protein 30 1.2
At5g48600 chromosome condensation protein 30 1.2
At5g25590 unknown protein (At5g25590) 30 1.2
At4g36520 trichohyalin like protein 30 1.2
>At3g13600 hypothetical protein
Length = 605
Score = 35.4 bits (80), Expect = 0.022
Identities = 31/124 (25%), Positives = 54/124 (43%), Gaps = 14/124 (11%)
Query: 63 DLNKFPKIEDSFEIESETIEHSLDVIKTVEDSLNATKIVEESDIESEEEGRPTIIRKKQI 122
D+ P ED F I + H + ++E+ L A K + D T++R + I
Sbjct: 369 DVKMSPTDEDEFSIYKQRSTHMRN--HSLEEDLEAEKTISFQDKVDPSGEEQTLMRNESI 426
Query: 123 WHEKDTELDSEECAPSTSRSFDSIENLHELNK-----------DAEEEDHFSHEREAMKN 171
K ++L++ E S S D I+++ + + EEE+ F E+E+M +
Sbjct: 427 -SRKQSDLETPEKMESFSTFGDEIQSVGSKSTKVSEDYDSGDDEEEEEEMFELEQESMPS 485
Query: 172 EVMS 175
E S
Sbjct: 486 EQSS 489
>At5g57200 putative protein
Length = 591
Score = 32.7 bits (73), Expect = 0.14
Identities = 25/82 (30%), Positives = 38/82 (45%), Gaps = 13/82 (15%)
Query: 102 EESDIESEEEGRPTIIRKKQIWHEKDTELDSEECAPSTSRSFDSIENLHELNKDA---EE 158
EE+ E+ E +P I +++ E E++ EE PS D + LHE+N A E+
Sbjct: 342 EENQNENTENDQPLIEEEEE---EPKEEIEVEEAKPSPLIDTDDLLGLHEINPKAAEIEQ 398
Query: 159 EDHFS-------HEREAMKNEV 173
+ FS HE A N +
Sbjct: 399 NNAFSLAIYPPGHETSAPSNSL 420
>At5g05750 DnaJ-like protein
Length = 294
Score = 32.3 bits (72), Expect = 0.18
Identities = 19/45 (42%), Positives = 28/45 (62%), Gaps = 1/45 (2%)
Query: 39 MGMKSHTSKEDDLKSHRG-KLKNHFDLNKFPKIEDSFEIESETIE 82
+G+KS+ S ED KS+R LK H D NK P E++F+ S+ +
Sbjct: 119 LGLKSNCSVEDLRKSYRKLSLKVHPDKNKAPGSEEAFKSVSKAFQ 163
>At3g57980 unknown protein
Length = 650
Score = 31.2 bits (69), Expect = 0.41
Identities = 25/106 (23%), Positives = 40/106 (37%), Gaps = 4/106 (3%)
Query: 78 SETIEHSLDVIKTVEDSLNATKIVEESDIESEEEGRPTIIRKKQIWHEKDTELDSEECAP 137
S + V +T++ V+E + EEEGRP+ +K I +K + AP
Sbjct: 434 SLAVRSPASVTETLKKKTRVVPTVDEKQVSEEEEGRPSDKDEKPIVSKK----MARGAAP 489
Query: 138 STSRSFDSIENLHELNKDAEEEDHFSHEREAMKNEVMSMLQIYFAG 183
ST++ S LN + +K V I +G
Sbjct: 490 STAKKVGSRNVKTSLNAGISNRGRSPNGSSVLKKSVQQKKGINTSG 535
>At3g28770 hypothetical protein
Length = 2081
Score = 31.2 bits (69), Expect = 0.41
Identities = 39/142 (27%), Positives = 61/142 (42%), Gaps = 19/142 (13%)
Query: 42 KSHTSKEDDLKSHRGKLKNHFDLNKFPK-IEDSF----------EIESETIEHSLDVIKT 90
K +K++ KS KLK NK K EDS E +S+T E + K
Sbjct: 973 KQEDNKKETTKSENSKLKEENKDNKEKKESEDSASKNREKKEYEEKKSKTKEEAKKEKKK 1032
Query: 91 VEDSLNATKIVEESDIESEEEGRPTIIRKKQIWHEKDTELDSEECAPSTSRSFDSIENLH 150
+D K EE + E+E + KK+ E++T+ E + + D E H
Sbjct: 1033 SQDKKREEKDSEERKSKKEKEESRDLKAKKK---EEETKEKKESENHKSKKKEDKKE--H 1087
Query: 151 ELNKDAEEEDHFSHEREAMKNE 172
E NK ++E+ ++E K+E
Sbjct: 1088 EDNKSMKKEE---DKKEKKKHE 1106
Score = 29.3 bits (64), Expect = 1.6
Identities = 30/132 (22%), Positives = 53/132 (39%), Gaps = 3/132 (2%)
Query: 43 SHTSKEDDLKSHRGKLKNHFDLNKFPKIEDSFEIESETIEHSLDVIKTVEDSLNATKIVE 102
S ++ + + + K K K K +D E ++ E K L A K E
Sbjct: 1007 SKNREKKEYEEKKSKTKEEAKKEK-KKSQDKKREEKDSEERKSKKEKEESRDLKAKKKEE 1065
Query: 103 ESDIESEEEGRPTIIRKKQIWHEKDTELDSEECAPSTSRSFDSIENLHELNKDAEE--ED 160
E+ + E E + ++ + HE + + EE + +S E +K E ED
Sbjct: 1066 ETKEKKESENHKSKKKEDKKEHEDNKSMKKEEDKKEKKKHEESKSRKKEEDKKDMEKLED 1125
Query: 161 HFSHEREAMKNE 172
S++++ KNE
Sbjct: 1126 QNSNKKKEDKNE 1137
>At2g27280 unknown protein
Length = 764
Score = 31.2 bits (69), Expect = 0.41
Identities = 24/76 (31%), Positives = 36/76 (46%), Gaps = 7/76 (9%)
Query: 91 VEDSLNATKIVEESDIESEEEGRPTIIR--KKQIWHEKDTELDSEECAPSTSRSFDSIE- 147
+E+ A K+ E+ E EE R + R KK+ EK+ DS E S S+S + +E
Sbjct: 200 LEEQRKAEKLEEQRKAEKLEELRKEVTRVEKKRKSPEKEVSPDSGEFGSSRSKSLEPLEA 259
Query: 148 ----NLHELNKDAEEE 159
+ E+ D EE
Sbjct: 260 EQAVSEKEMGSDGTEE 275
>At2g21800 hypothetical protein
Length = 475
Score = 31.2 bits (69), Expect = 0.41
Identities = 28/92 (30%), Positives = 37/92 (39%), Gaps = 14/92 (15%)
Query: 92 EDSLNATKIVEESDIESEEEGRPTIIRKKQIWHEKDTELDSEECAPSTSRSFD------- 144
+ SL+ I +SD E E+ G + RKKQ K T L S + P S D
Sbjct: 143 QSSLSKDAIEVDSDHEKEDTGVEKMGRKKQTITSKSTSL-SADSLPKKKMSKDEKTRAAE 201
Query: 145 ------SIENLHELNKDAEEEDHFSHEREAMK 170
E L + AE+ +H ERE K
Sbjct: 202 EKKLQKEQEKLQKAASKAEDAEHKKLEREKQK 233
>At3g50630 unknown protein
Length = 209
Score = 30.8 bits (68), Expect = 0.53
Identities = 29/118 (24%), Positives = 53/118 (44%), Gaps = 9/118 (7%)
Query: 68 PKIEDSFEIESETIEHSLDVIKTVEDSLNATKIVEESDIES----EEEGRPTIIRKKQIW 123
P +E+ +IE E + S+ T E+ + + +E D+E + E + I
Sbjct: 74 PPVEEQCQIEEE--DSSVSCCSTSEEK--SKRRIEFVDLEENNGDDRETETSWIYDDLNK 129
Query: 124 HEKDTELDSEECAPSTSRSFDSI-ENLHELNKDAEEEDHFSHEREAMKNEVMSMLQIY 180
E+ +DS A S + ++LHE K+AE ED F + ++N+++ Y
Sbjct: 130 SEESMNMDSSSVAVEDVESRRRLRKSLHETVKEAELEDFFQVAEKDLRNKLLECSMKY 187
>At2g36420 unknown protein
Length = 439
Score = 30.8 bits (68), Expect = 0.53
Identities = 19/66 (28%), Positives = 30/66 (44%), Gaps = 9/66 (13%)
Query: 107 ESEEEGRPTIIRKKQIWHEKDTEL-DSEECAPSTSRSFDSIENLHELNKDAEEEDHFSHE 165
E E+ + K + E+D E D E+C+P + L L ++ E+EDH HE
Sbjct: 221 EDEDSDETESLEKVRGQEEEDKEEEDKEQCSP--------VSVLDPLEEEEEDEDHHQHE 272
Query: 166 REAMKN 171
+ N
Sbjct: 273 PDPPNN 278
>At4g26630 unknown protein
Length = 763
Score = 30.4 bits (67), Expect = 0.70
Identities = 24/86 (27%), Positives = 39/86 (44%), Gaps = 4/86 (4%)
Query: 89 KTVEDSLNATKIVEES----DIESEEEGRPTIIRKKQIWHEKDTELDSEECAPSTSRSFD 144
K+ + S ATK+V++S D ESEEE ++++ EK+ + + E +S D
Sbjct: 500 KSQKKSEEATKVVKKSLAHSDDESEEEKEEEEKQEEEKAEEKEEKKEEENENGIPDKSED 559
Query: 145 SIENLHELNKDAEEEDHFSHEREAMK 170
E + E E+H E K
Sbjct: 560 EAPQPSESEEKDESEEHSEEETTKKK 585
>At3g53640 protein kinase -like protein
Length = 642
Score = 30.4 bits (67), Expect = 0.70
Identities = 17/83 (20%), Positives = 37/83 (44%)
Query: 34 SSSHYMGMKSHTSKEDDLKSHRGKLKNHFDLNKFPKIEDSFEIESETIEHSLDVIKTVED 93
SSS G ++ + D RG+ ++ D + D + E LD +K+ +
Sbjct: 100 SSSRETGRENERERRKDQDRDRGRREDQSDQEIYKSGGDGYGEVRHDAEDDLDSLKSHKP 159
Query: 94 SLNATKIVEESDIESEEEGRPTI 116
+ VE S+++++++G +
Sbjct: 160 NGETQGNVERSEVDNDDDGEDVV 182
>At1g10890 unknown protein
Length = 592
Score = 30.4 bits (67), Expect = 0.70
Identities = 38/145 (26%), Positives = 66/145 (45%), Gaps = 11/145 (7%)
Query: 34 SSSHYMGMKSHTSKEDDLKSHRG-KLKNHFDLNKFPKIEDSFE-IESETIEHSLDVI-KT 90
SS+ +S + + K+ G KLK + K + E + IE ET++ + I K
Sbjct: 38 SSTPSPAKRSPAATLESAKNRNGEKLKREEEERKRRQREAELKLIEEETVKRVEEAIRKK 97
Query: 91 VEDSLNATKIVEESDIESEEEGRPTIIRKKQIWHEKDTELDSE-ECAPSTSRSFDSIENL 149
VE+SL + KI E + EEGR K++ E +L+ E E + ++ + E
Sbjct: 98 VEESLQSEKIKMEI-LTLLEEGR------KRLNEEVAAQLEEEKEASLIEAKEKEEREQQ 150
Query: 150 HELNKDAEEEDHFSHEREAMKNEVM 174
+ ++ E++ EA + E M
Sbjct: 151 EKEERERIAEENLKRVEEAQRKEAM 175
>At1g03050 putative protein destination factor
Length = 599
Score = 30.4 bits (67), Expect = 0.70
Identities = 27/94 (28%), Positives = 44/94 (46%), Gaps = 8/94 (8%)
Query: 35 SSHYMGMKSHTSKEDDLKSHRGKLKNHFDLNKFPKIEDSFEIESETIEHSLDVIKTVEDS 94
SS Y ++ T K+ DL + K+ + K K S E + + E + + ++
Sbjct: 301 SSEYPEIEKITQKKLDLMDEFIRDKSALEHTKQSKSVKS-EADEDDDEARTEEVNEEQED 359
Query: 95 LNATKIV------EESDIESEEEGR-PTIIRKKQ 121
+NA K + EE D++ EEE + II KKQ
Sbjct: 360 MNAIKALPEPPPKEEDDVKPEEEAKEEVIIEKKQ 393
>At5g61330 unknown protein
Length = 436
Score = 30.0 bits (66), Expect = 0.91
Identities = 30/127 (23%), Positives = 58/127 (45%), Gaps = 13/127 (10%)
Query: 46 SKEDDLKSHRGKLKNHFDLNKFPKIEDSFEIESETIEHSLDVIKTVEDSLNATKIVEESD 105
S+ +D+ S + LK D N+ ++ D E + E D ++ ED T+ +E D
Sbjct: 15 SESEDI-SDQENLKAESD-NEDDQLPDGIE-DDEVDSMEDDEGESEEDDEGDTEEDDEGD 71
Query: 106 IESEEEGRPTIIRKKQIWHEKDTELDSEECAPSTSRSFDSIENLHELNKDAEEEDHFSHE 165
E ++EG +++D + +SE+ + +S + ++ NKDA+ E+
Sbjct: 72 SEEDDEGE----------NKEDEDGESEDFEDGNDKESESGDEGNDDNKDAQMEELEKEV 121
Query: 166 REAMKNE 172
+E E
Sbjct: 122 KELRSQE 128
>At3g22860 eukaryotic translation initiation factor 3 (subunit 8)
Length = 800
Score = 30.0 bits (66), Expect = 0.91
Identities = 27/93 (29%), Positives = 37/93 (39%), Gaps = 8/93 (8%)
Query: 2 ENCKHTNMQYIYRVAPNGIYTFTSFLFPALLFSSSHYMGMKSHTSKEDDLKSHRGKLKNH 61
E + N Q V +Y T + L + K TS L + R KLK +
Sbjct: 79 ETFEKLNKQISKSVKTPTLYIKTLVMLEDFLNEDNMKTKEKMSTSNSKALNAMRQKLKKN 138
Query: 62 F-----DLNKF---PKIEDSFEIESETIEHSLD 86
D+ +F P+IED E E E +E S D
Sbjct: 139 NLQYQEDIKRFRESPEIEDDDEYEEEVVEDSAD 171
>At2g14470 pseudogene
Length = 1265
Score = 30.0 bits (66), Expect = 0.91
Identities = 16/47 (34%), Positives = 21/47 (44%)
Query: 23 FTSFLFPALLFSSSHYMGMKSHTSKEDDLKSHRGKLKNHFDLNKFPK 69
F L L +H H ED LK R + KN D+++FPK
Sbjct: 720 FAMLLLSDSLSRPAHVWSQTWHILAEDILKKKRDEFKNPEDIDEFPK 766
>At5g55660 putative protein
Length = 759
Score = 29.6 bits (65), Expect = 1.2
Identities = 23/78 (29%), Positives = 39/78 (49%), Gaps = 7/78 (8%)
Query: 89 KTVEDSLNATKIVEESDIESEEEGRPTIIRKKQIWHEKDTELD-------SEECAPSTSR 141
KT E + K V SD ESEEE +K+ E++ E + SE+ AP S
Sbjct: 491 KTEEATRTNKKSVAHSDDESEEEKEDDEEEEKEQEVEEEEEENENGIPDKSEDEAPQLSE 550
Query: 142 SFDSIENLHELNKDAEEE 159
S +++E+ E ++ +++
Sbjct: 551 SEENVESEEESEEETKKK 568
>At5g48600 chromosome condensation protein
Length = 1241
Score = 29.6 bits (65), Expect = 1.2
Identities = 31/134 (23%), Positives = 55/134 (40%), Gaps = 25/134 (18%)
Query: 58 LKNHFDLNKFPKIEDSFEIESETIEHS----LDVIKTVEDSLNATKIVEESDIESEEEGR 113
L++ NK+ + D + ET+ S + ++K E E ++E ++
Sbjct: 196 LEDIIGTNKYVEKIDELNKQLETLNESRSGVVQMVKLAEK--------ERDNLEGLKDEA 247
Query: 114 PTIIRKKQI---WHEKDTELDSEECAPSTSRSFDSIENLHELNKDA--------EEEDHF 162
T + K+ W EK T++ E+ + DS++NL KD EE F
Sbjct: 248 ETYMLKELSHLKWQEKATKMAYEDTVAKITEQRDSLQNLENSLKDERVKMDESNEELKKF 307
Query: 163 S--HEREAMKNEVM 174
HE+ + EV+
Sbjct: 308 ESVHEKHKKRQEVL 321
>At5g25590 unknown protein (At5g25590)
Length = 775
Score = 29.6 bits (65), Expect = 1.2
Identities = 24/66 (36%), Positives = 28/66 (42%), Gaps = 11/66 (16%)
Query: 102 EESDIESEEEGRPTIIRKKQIWHEKDTELDSEECAPSTSRSFDSIENLHELNKDAEEEDH 161
EE D E EEE R I RKK + EE P T E + E ++ EEED
Sbjct: 232 EEDDEEEEEEERSGIYRKK-----SGSGKVVEEMEPKTP------EKVEEEEEEDEEEDE 280
Query: 162 FSHERE 167
E E
Sbjct: 281 EEEEEE 286
>At4g36520 trichohyalin like protein
Length = 1432
Score = 29.6 bits (65), Expect = 1.2
Identities = 26/83 (31%), Positives = 38/83 (45%), Gaps = 3/83 (3%)
Query: 92 EDSLNATKIVEESDIESEEEGRPTIIRKKQIWHEKDT--ELDSEECAPSTSRSFDSIENL 149
E N K E S ES+E+ R +K K+ E S E T +SI+N
Sbjct: 885 EHQENERKQHEYSGEESDEKERDACEMEKTCETTKEAHGEQSSNESLSDTLEENESIDND 944
Query: 150 HELNKDAEEEDHFSHEREAMKNE 172
+NK +EE+ + +RE+M E
Sbjct: 945 VSVNKQKKEEEG-TRQRESMSAE 966
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.315 0.132 0.372
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,907,620
Number of Sequences: 26719
Number of extensions: 224191
Number of successful extensions: 909
Number of sequences better than 10.0: 76
Number of HSP's better than 10.0 without gapping: 13
Number of HSP's successfully gapped in prelim test: 64
Number of HSP's that attempted gapping in prelim test: 870
Number of HSP's gapped (non-prelim): 101
length of query: 209
length of database: 11,318,596
effective HSP length: 95
effective length of query: 114
effective length of database: 8,780,291
effective search space: 1000953174
effective search space used: 1000953174
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0119.3