BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0117b.14
(458 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g20210 putative protein 43 3e-04
At1g67020 hypothetical protein 41 0.002
At5g25590 unknown protein (At5g25590) 38 0.013
At4g10580 putative reverse-transcriptase -like protein 37 0.017
At5g20875 unknown protein 36 0.051
At3g31970 hypothetical protein 34 0.19
At3g29480 hypothetical protein 34 0.19
At5g41950 unknown protein 33 0.25
At5g11370 putative protein 33 0.43
At1g79280 hypothetical protein 33 0.43
At4g04310 putative transposon protein 32 0.56
At2g26110 unknown protein 32 0.56
At2g04670 putative retroelement pol polyprotein 32 0.56
At3g18610 unknown protein 32 0.73
At2g14650 putative retroelement pol polyprotein 32 0.73
At5g14830 putative protein 32 0.95
At4g07350 30 2.1
At4g03650 putative reverse transcriptase 30 2.1
At1g22530 unknown protein 30 2.1
At5g60980 ras-GTPase-activating protein SH3-domain binding prote... 30 2.8
>At5g20210 putative protein
Length = 256
Score = 43.1 bits (100), Expect = 3e-04
Identities = 20/68 (29%), Positives = 34/68 (49%)
Query: 3 PEFDGREAYGWLIMVEQHCEAKGVSEEEKFSGAEKALTGEAFMWWFCWRRRNQKATWWEF 62
P FDG + WL ++E + + E K A +++ G+A +W C R+ N TW +
Sbjct: 68 PSFDGDHLFIWLEIIEVYFTLQLAPEVCKVDTAFRSMEGDALLWLQCLRQENPTLTWSQL 127
Query: 63 VEALLRKF 70
L+ +F
Sbjct: 128 KTELIEEF 135
>At1g67020 hypothetical protein
Length = 659
Score = 40.8 bits (94), Expect = 0.002
Identities = 30/125 (24%), Positives = 49/125 (39%), Gaps = 7/125 (5%)
Query: 3 PEFDGREAYGWLIMVEQHCEAKGVSEEEKFSGAEKALTGEAFMWWFCWRRRNQKATWWEF 62
P FDG Y W VE+ + +K +L G A W+ + W F
Sbjct: 113 PVFDGSGVYEWFSKVERFFRVGRYQDSDKLDLVALSLEGVALKWFLREMSTLEFRDWNSF 172
Query: 63 VEALLRKFEPELEPYMPEPVQD-------SEEEEIPGKQEVLEAERRTVVVEAKPEANSV 115
+ LL +F+P PE + + E E +E++E + +V ++ PE +
Sbjct: 173 EQRLLARFDPVKIHSSPEVLMPTTLIHVVAHETESRCTEELIEEDESSVRKKSNPEIVLI 232
Query: 116 LLAKS 120
KS
Sbjct: 233 TQKKS 237
>At5g25590 unknown protein (At5g25590)
Length = 775
Score = 37.7 bits (86), Expect = 0.013
Identities = 32/109 (29%), Positives = 42/109 (38%), Gaps = 15/109 (13%)
Query: 69 KFEPELEPYMPEPVQDSEEEEIPGKQEVLEAERRTVVVEAK---------------PEAN 113
K E+EP PE V++ EEE+ +E E E VVVE K P
Sbjct: 255 KVVEEMEPKTPEKVEEEEEEDEEEDEEEEEEEEEEVVVEVKKKKKGKAKIEHSSTAPPEF 314
Query: 114 SVLLAKSEIKPYSPENSEMEASRKDSEFAMAAEQTAEVSRCQPVTVVDY 162
+AK+ S N D F A+E EVS+ T + Y
Sbjct: 315 RRAVAKTSAAASSSVNLMKILDEIDDRFLKASECAQEVSKMLEATRLHY 363
>At4g10580 putative reverse-transcriptase -like protein
Length = 1240
Score = 37.4 bits (85), Expect = 0.017
Identities = 23/84 (27%), Positives = 39/84 (46%), Gaps = 4/84 (4%)
Query: 9 EAYGWLIMVEQHCEAKGVSEEEKFSGAEKALTGEAFMWW--FCWRRRNQKATWWEFVEAL 66
EA W V ++ + E + A L G+A +WW RRR +W +FV
Sbjct: 160 EADSWRSRVGRNFGSSRCPAEYRVDLAVHFLEGDAHLWWRSVTARRRQTDMSWADFVAEF 219
Query: 67 LRKFEPE--LEPYMPEPVQDSEEE 88
K+ P+ L+PY + ++D + +
Sbjct: 220 KAKYFPQEALDPYAGQGMEDDQAQ 243
>At5g20875 unknown protein
Length = 272
Score = 35.8 bits (81), Expect = 0.051
Identities = 19/68 (27%), Positives = 31/68 (44%), Gaps = 1/68 (1%)
Query: 3 PEFDGREAYGWLIMVEQHCEAKGVSEEEKFSGAEKALTGEAFMWWFCWRRRNQKATWWEF 62
P F+G WL E++ E ++ EK ++ G A + WF RN W +F
Sbjct: 117 PVFEGPNVNSWLTRAERYFEFGSFTDAEKIQLVYMSVEGRA-LCWFNLENRNPFVDWNDF 175
Query: 63 VEALLRKF 70
+L++F
Sbjct: 176 KARVLQRF 183
>At3g31970 hypothetical protein
Length = 1329
Score = 33.9 bits (76), Expect = 0.19
Identities = 29/99 (29%), Positives = 39/99 (39%), Gaps = 13/99 (13%)
Query: 9 EAYGWLIMVEQHCEAKGVSEEEKFSGAEKALTGEAFMWW--FCWRRRNQKATWWEFVEAL 66
EA W VE + + E + A L G+A +WW RRR +W +FV
Sbjct: 166 EADSWKSRVEHNFGSSRCPAEYRVDLAVHFLEGDAHLWWRSVTARRRQAHMSWADFVAEF 225
Query: 67 LRKFEPELEPYMPEPVQDSEE----EEIPGKQEVLEAER 101
K Y P+ D E E G++ V E ER
Sbjct: 226 NAK-------YFPQEALDRMEARFLELTQGERSVREYER 257
>At3g29480 hypothetical protein
Length = 718
Score = 33.9 bits (76), Expect = 0.19
Identities = 20/67 (29%), Positives = 30/67 (43%), Gaps = 2/67 (2%)
Query: 9 EAYGWLIMVEQHCEAKGVSEEEKFSGAEKALTGEAFMWW--FCWRRRNQKATWWEFVEAL 66
EA W VE++ + E + A L G+A +WW RRR +W +FV
Sbjct: 59 EADSWRSRVERNFGSSRCPVEYRVDLAVHFLEGDAHLWWKSVTTRRRQANMSWADFVAEF 118
Query: 67 LRKFEPE 73
K+ P+
Sbjct: 119 NAKYFPQ 125
>At5g41950 unknown protein
Length = 565
Score = 33.5 bits (75), Expect = 0.25
Identities = 25/58 (43%), Positives = 35/58 (60%), Gaps = 12/58 (20%)
Query: 72 PELEPYM-PEPVQ----DSEEEEIPGKQEVLEAERRTVVVEAKPEANSVLLAKSEIKP 124
PE+E + PE VQ D++ EE+ + EV E +TVV +AKPE A+SE+KP
Sbjct: 42 PEIEADLTPEEVQSEVTDAKPEEV--QSEVKPEEVKTVVTDAKPEE-----AQSEVKP 92
>At5g11370 putative protein
Length = 311
Score = 32.7 bits (73), Expect = 0.43
Identities = 33/139 (23%), Positives = 57/139 (40%), Gaps = 12/139 (8%)
Query: 156 PVTVVDYIDCTLTAKGEKIDAEGEIKIKRLQEG-SSMEKWQI-CSIHGRKKV--QPIVLP 211
P + + Y+ + E A G +K+L + SS+++ + ++ + + IV
Sbjct: 134 PQSSLSYLIVNMPKLEEADIARGSHDVKKLLKSISSVKRLSLYLEVNNNEDLYGDDIVFN 193
Query: 212 QISDLGFDWERRPPRKPPDSCLCTAVTANRLPDTFFCWTEVFIQTSVLCSSMVAIQLETK 271
++ L F R PR A DT FCW ++ L SS+ + T
Sbjct: 194 ELEHLKFRISRAVPRS-------MANVVGDSMDTLFCWKQMIDVPQCLLSSLQTFKWRTY 246
Query: 272 -PPDRSGGSSHFILGDSCR 289
P + G + +IL SCR
Sbjct: 247 FPTEEEKGLATYILAKSCR 265
>At1g79280 hypothetical protein
Length = 2111
Score = 32.7 bits (73), Expect = 0.43
Identities = 26/128 (20%), Positives = 47/128 (36%), Gaps = 11/128 (8%)
Query: 71 EPELEPYMPEPVQDSEEEEIPGKQEVLEAERRTVVVEAKPEANSVLLAKSEIKPYSPENS 130
+PE P + + ++E GKQ V +P V +++ P+
Sbjct: 1728 KPEESPKVDVDMPEAEGTGDEGKQPAAHEPESQVTTSVRPVQTLVRKRQADSLVSEPQQD 1787
Query: 131 EMEASRKDSEFAMAAEQTAEVSRCQPVT-----------VVDYIDCTLTAKGEKIDAEGE 179
+ SE A A + A+ S P T + + +D T T G+ + E E
Sbjct: 1788 SLTQGETSSEIAPPASKKAKGSESHPDTSEGENLAKEPAIDELMDATTTTDGDNEETEAE 1847
Query: 180 IKIKRLQE 187
++ +E
Sbjct: 1848 NAEEKTEE 1855
>At4g04310 putative transposon protein
Length = 1011
Score = 32.3 bits (72), Expect = 0.56
Identities = 36/177 (20%), Positives = 69/177 (38%), Gaps = 22/177 (12%)
Query: 4 EFDGREAY-GWLIMVEQHCEAKGVSEEEKFSGAEKALTGEAFMWWF------CWRRRNQK 56
+F G++ Y W +++ K E +F A LTG+A+ WW + +
Sbjct: 223 KFSGKQDYLEWEKNMDEWFSYKNFLSEMRFVCALSHLTGDAYKWWLQEVDDRLYYKEPPI 282
Query: 57 ATWWEFVEALLRKFEPELEPYMPE--------PVQDSEEEEIP--GKQEVLEAERRTVVV 106
W +F + L K+ P+ P+ Q+ E+ P K + E + + ++
Sbjct: 283 TLWRDFKKLLRNKYAPQALECTPKGNPKIQGLAAQEKEQGLAPYSKKNPIAEQQLKDEIL 342
Query: 107 EAKPEANSVLLAKSEIKP-YSPENSEMEASRKDSEFAMAAEQ----TAEVSRCQPVT 158
+ N KS +P E + + A + EQ ++ +S+ + VT
Sbjct: 343 KILNAYNKPKKVKSTSQPKMVTEEVVVHKQNRQPSLASSGEQKQCKSSNLSKSRGVT 399
>At2g26110 unknown protein
Length = 309
Score = 32.3 bits (72), Expect = 0.56
Identities = 30/115 (26%), Positives = 52/115 (45%), Gaps = 10/115 (8%)
Query: 86 EEEEIPGKQEVLEAERRTVVVEAKPEANSVLLAKSEIKPYS-------PENSEMEASRKD 138
+E+E+ +QE E E+ V +K N V KS+ +P + P+ + AS K
Sbjct: 181 QEQEVKEEQE--EEEQSLEEVYSKLNLNHVARTKSDTEPAAGIRPPKLPKKMKKSASTK- 237
Query: 139 SEFAMAAEQTAEVSRCQPVTVVDYIDCTLTAKGEKIDAEGEIKIKRLQEGSSMEK 193
S F+ E V +P TV T+ E++DA+ + I R + +++
Sbjct: 238 SPFSHFQEDEISVEARRPATVKVPRVTTVEEADEEVDAKADDFINRFKHQLKLQR 292
>At2g04670 putative retroelement pol polyprotein
Length = 1411
Score = 32.3 bits (72), Expect = 0.56
Identities = 19/67 (28%), Positives = 30/67 (44%), Gaps = 2/67 (2%)
Query: 9 EAYGWLIMVEQHCEAKGVSEEEKFSGAEKALTGEAFMWW--FCWRRRNQKATWWEFVEAL 66
EA W V+++ + E + A L G+A +WW RRR +W +FV
Sbjct: 146 EADSWRSRVQRNFGSSRCPAEYRVDLAVHFLEGDAHLWWRSVTARRRQADMSWADFVAEF 205
Query: 67 LRKFEPE 73
K+ P+
Sbjct: 206 NAKYFPQ 212
>At3g18610 unknown protein
Length = 636
Score = 32.0 bits (71), Expect = 0.73
Identities = 25/96 (26%), Positives = 46/96 (47%), Gaps = 7/96 (7%)
Query: 85 SEEEEIPGKQEVLEAERRTVVVE-AKPEANSVLLAKSEIKPYSPENSEMEASRKDSEFAM 143
S +++ +E + +++T V+E AK E++S S + +P E +KDS
Sbjct: 182 SSDDDSSSDEETVPMKKQTAVLEKAKAESSSSDDGSSSDEEPTPAKKEPIVVKKDSSDES 241
Query: 144 AAEQTAEVSRCQPVTVVDYIDCTLTAKGEKIDAEGE 179
++++ V + +P TVV AK E +E E
Sbjct: 242 SSDEETPVVKKKPTTVVK------DAKAESSSSEEE 271
>At2g14650 putative retroelement pol polyprotein
Length = 1328
Score = 32.0 bits (71), Expect = 0.73
Identities = 19/66 (28%), Positives = 29/66 (43%), Gaps = 2/66 (3%)
Query: 10 AYGWLIMVEQHCEAKGVSEEEKFSGAEKALTGEAFMWW--FCWRRRNQKATWWEFVEALL 67
A W VE++ + E + A L G+A +WW RRR +W +FV
Sbjct: 165 ADSWRSRVERNFGSSRCPAEYRIDLAVHFLEGDAHLWWRSVTARRRQADISWADFVAEFN 224
Query: 68 RKFEPE 73
K+ P+
Sbjct: 225 AKYFPQ 230
>At5g14830 putative protein
Length = 757
Score = 31.6 bits (70), Expect = 0.95
Identities = 15/45 (33%), Positives = 23/45 (50%)
Query: 28 EEEKFSGAEKALTGEAFMWWFCWRRRNQKATWWEFVEALLRKFEP 72
E +K A ALTG A WW + + +TW +F + +F+P
Sbjct: 620 ETQKLEQAITALTGTAINWWQIAQYPEKISTWKDFRDKFKVRFKP 664
>At4g07350
Length = 330
Score = 30.4 bits (67), Expect = 2.1
Identities = 16/47 (34%), Positives = 24/47 (51%), Gaps = 1/47 (2%)
Query: 2 FPEFDGREAY-GWLIMVEQHCEAKGVSEEEKFSGAEKALTGEAFMWW 47
F +F G+E Y W +++ K E +F A LTG+A+ WW
Sbjct: 89 FFKFSGKEDYLEWEKNMDEWFSYKNFLSEMRFVCALSHLTGDAYKWW 135
>At4g03650 putative reverse transcriptase
Length = 839
Score = 30.4 bits (67), Expect = 2.1
Identities = 18/66 (27%), Positives = 28/66 (42%), Gaps = 2/66 (3%)
Query: 9 EAYGWLIMVEQHCEAKGVSEEEKFSGAEKALTGEAFMWW--FCWRRRNQKATWWEFVEAL 66
EA W VE++ + E + L G+A +WW RRR +W +F+
Sbjct: 164 EADSWRSQVERNFGSSRCPAEYRVDLTVHFLEGDAHLWWRSVTARRRQADMSWADFMAEF 223
Query: 67 LRKFEP 72
K+ P
Sbjct: 224 NAKYFP 229
>At1g22530 unknown protein
Length = 683
Score = 30.4 bits (67), Expect = 2.1
Identities = 26/91 (28%), Positives = 36/91 (38%), Gaps = 4/91 (4%)
Query: 71 EPELEPYMPEPVQDSEEEEIPGKQEVLEAERRTVVVEAKPEANSVLLAKSEIKPYSPENS 130
E LE E + + + K+E + A +V E K E V A E KP +P
Sbjct: 148 EKSLEAETKEEEKSAAPATVETKKEEILAAPAPIVAETKKEETPVAPAPVETKPAAP--- 204
Query: 131 EMEASRKDSEFAMAAEQTAEVSRCQPVTVVD 161
+ A K E AA T E + V V+
Sbjct: 205 -VVAETKKEEILPAAPVTTETKVEEKVVPVE 234
>At5g60980 ras-GTPase-activating protein SH3-domain binding protein
- like
Length = 460
Score = 30.0 bits (66), Expect = 2.8
Identities = 20/61 (32%), Positives = 32/61 (51%), Gaps = 5/61 (8%)
Query: 64 EALLRKFEPELEPYMPEPVQDSEEEEIPGKQEVLEAERRT--VVVEAKPEANSVLLAKSE 121
E ++ EPE+EP EPV EEE++ EV + + VVV+ +P ++ +E
Sbjct: 160 ERVVVSHEPEVEP---EPVASIEEEDLDNVAEVYDPSDKDEGVVVDVEPIEPPTQISHNE 216
Query: 122 I 122
I
Sbjct: 217 I 217
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.320 0.135 0.416
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,812,424
Number of Sequences: 26719
Number of extensions: 465192
Number of successful extensions: 1336
Number of sequences better than 10.0: 39
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 31
Number of HSP's that attempted gapping in prelim test: 1317
Number of HSP's gapped (non-prelim): 45
length of query: 458
length of database: 11,318,596
effective HSP length: 103
effective length of query: 355
effective length of database: 8,566,539
effective search space: 3041121345
effective search space used: 3041121345
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0117b.14