BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0115.1
(204 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g15310 unknown protein 129 8e-31
At1g42690 unknown protein 98 3e-21
At5g28700 putative protein 85 2e-17
At1g24370 hypothetical protein 70 8e-13
At2g13770 hypothetical protein 69 2e-12
At2g14730 hypothetical protein 40 8e-04
At5g12010 putative protein 35 0.021
At4g29780 unknown protein 33 0.079
At1g47550 hypothetical protein 32 0.30
At1g36675 putative protein 32 0.30
At5g04460 putative protein 30 0.67
At5g35580 serine/threonine protein kinase-like 29 1.9
At4g21130 hypothetical protein 29 1.9
At3g63430 unknown protein 28 2.5
At4g02750 hypothetical protein 28 4.3
At4g07620 putative athila transposon protein 27 5.7
At1g23940 gamma-adaptin, putative 27 9.6
>At3g15310 unknown protein
Length = 415
Score = 129 bits (325), Expect = 8e-31
Identities = 73/172 (42%), Positives = 95/172 (54%)
Query: 32 SECPFDLAAYIQNSQIEEAYVLNRFRERRRKIREDTAPRSRKYLGRDHTAANQRLIGDYF 91
S PF+ A Q+ + N F E R R Y R + L DYF
Sbjct: 5 SRTPFNDAMEESFDQLLDQQFENVFVELSNSEEVAKKNRKRAYFDRKREEGHVLLWNDYF 64
Query: 92 ANEPTYDDAMFRRRYRMQKHLFLRIVDDLSSSDNYFTQRVDAANKQGISPLAKCTTAMRM 151
++ + FRRR+RM+K LFLRIVD LSS +F R DA + G SP+ KCT A+R+
Sbjct: 65 SDNAIFPLQTFRRRFRMKKPLFLRIVDRLSSELMFFQHRRDATGRFGHSPIQKCTAAIRL 124
Query: 152 LAYGVAADAVDEYIKIGGTTALECLRRFCKGIIQLYEQQYLRAPVRPEILSL 203
LAYG A+DAVDEY+++G TTA+ CL F KGII + +YLRAP + L
Sbjct: 125 LAYGYASDAVDEYLRMGETTAMSCLENFTKGIISFFGDEYLRAPTATNLRRL 176
>At1g42690 unknown protein
Length = 333
Score = 97.8 bits (242), Expect = 3e-21
Identities = 58/155 (37%), Positives = 84/155 (53%), Gaps = 17/155 (10%)
Query: 64 REDTAPRSRK-YLGRDHTAANQRLIGDYFANEPTYDDAMFRRRYRMQKHLFLRIVDDLSS 122
R+ PR +K Y+ R+ + +L+ DYF PTY +FRRR+RM K LF+RIV+ S+
Sbjct: 32 RQRVKPRKKKLYIERNREEGHIQLVNDYFTENPTYPPHIFRRRFRMNKSLFMRIVERFSN 91
Query: 123 SDNYFTQRVDAANKQGISPLAKCTTAMRMLAYGVAADAV------DEYIKI--------- 167
YF QR DA + G S L K T A+RMLAYG+AADA D+ I++
Sbjct: 92 EVPYFKQRRDATRRLGFSALQKSTAAIRMLAYGIAADAYLRRPTRDDLIRLLHIGEQRGF 151
Query: 168 -GGTTALECLRRFCKGIIQLYEQQYLRAPVRPEIL 201
G +++C+ K ++ QY R +P I+
Sbjct: 152 PGMIGSIDCMHWEWKNCPTAWKGQYTRGSGKPTIV 186
>At5g28700 putative protein
Length = 292
Score = 85.1 bits (209), Expect = 2e-17
Identities = 43/78 (55%), Positives = 54/78 (69%)
Query: 96 TYDDAMFRRRYRMQKHLFLRIVDDLSSSDNYFTQRVDAANKQGISPLAKCTTAMRMLAYG 155
TY +FR R+RM K LF+RIV+ S+ YF QR DA + S L K T A+RMLAYG
Sbjct: 33 TYPPHIFRHRFRMNKPLFMRIVERFSNEVPYFKQRRDATGRLDFSALQKSTAAIRMLAYG 92
Query: 156 VAADAVDEYIKIGGTTAL 173
+AADAVDEY++IG +T L
Sbjct: 93 IAADAVDEYLRIGESTLL 110
>At1g24370 hypothetical protein
Length = 413
Score = 70.1 bits (170), Expect = 8e-13
Identities = 32/58 (55%), Positives = 44/58 (75%)
Query: 146 TTAMRMLAYGVAADAVDEYIKIGGTTALECLRRFCKGIIQLYEQQYLRAPVRPEILSL 203
T A RMLAYGV AD+ DEYIKIG +TALE L+RFC+ I++++ +YLR+P ++ L
Sbjct: 2 TAAFRMLAYGVPADSTDEYIKIGESTALESLKRFCRAIVEVFACRYLRSPDANDVARL 59
>At2g13770 hypothetical protein
Length = 244
Score = 68.6 bits (166), Expect = 2e-12
Identities = 32/58 (55%), Positives = 43/58 (73%)
Query: 146 TTAMRMLAYGVAADAVDEYIKIGGTTALECLRRFCKGIIQLYEQQYLRAPVRPEILSL 203
T A RMLAYGV AD+ DEYIKIG +TALE L+RFC+ I++++ YLR+P ++ L
Sbjct: 2 TAAFRMLAYGVPADSTDEYIKIGESTALESLKRFCRAIVEVFACCYLRSPDANDVARL 59
>At2g14730 hypothetical protein
Length = 117
Score = 40.0 bits (92), Expect = 8e-04
Identities = 17/44 (38%), Positives = 28/44 (63%)
Query: 160 AVDEYIKIGGTTALECLRRFCKGIIQLYEQQYLRAPVRPEILSL 203
AVD+Y++IG T + C+ + II L+ ++YLR P R ++ L
Sbjct: 19 AVDKYLRIGENTLMSCMIHSVEAIIYLFGKEYLRRPTRQDLKRL 62
>At5g12010 putative protein
Length = 502
Score = 35.4 bits (80), Expect = 0.021
Identities = 25/99 (25%), Positives = 44/99 (44%), Gaps = 4/99 (4%)
Query: 97 YDDAMFRRRYRMQKHLFLRIVDDLSSSDNYFTQRVDAANKQGISPLAKCTTAMRMLAYGV 156
Y + F++ +RM K F I D+L+S+ + D A + I + + LA G
Sbjct: 170 YPEEDFKKAFRMSKSTFELICDELNSA----VAKEDTALRNAIPVRQRVAVCIWRLATGE 225
Query: 157 AADAVDEYIKIGGTTALECLRRFCKGIIQLYEQQYLRAP 195
V + +G +T + + CK I + +YL+ P
Sbjct: 226 PLRLVSKKFGLGISTCHKLVLEVCKAIKDVLMPKYLQWP 264
>At4g29780 unknown protein
Length = 540
Score = 33.5 bits (75), Expect = 0.079
Identities = 26/110 (23%), Positives = 46/110 (41%), Gaps = 4/110 (3%)
Query: 93 NEPTYDDAMFRRRYRMQKHLFLRIVDDLSSSDNYFTQRVDAANKQGISPLAKCTTAMRML 152
+ P + + FRR +RM K F I ++L ++ + + + I + + L
Sbjct: 204 SRPDFPEDEFRREFRMSKSTFNLICEELDTT----VTKKNTMLRDAIPAPKRVGVCVWRL 259
Query: 153 AYGVAADAVDEYIKIGGTTALECLRRFCKGIIQLYEQQYLRAPVRPEILS 202
A G V E +G +T + + C+ I + +YL P EI S
Sbjct: 260 ATGAPLRHVSERFGLGISTCHKLVIEVCRAIYDVLMPKYLLWPSDSEINS 309
>At1g47550 hypothetical protein
Length = 861
Score = 31.6 bits (70), Expect = 0.30
Identities = 28/131 (21%), Positives = 49/131 (37%), Gaps = 3/131 (2%)
Query: 24 QISQKMDPSECPFDLAAYIQNSQIEEAYVLNRFRERRRKIREDTAPRSRKYLGRDHTAAN 83
++ + ++ P + AA + +EA +L + A R + D AN
Sbjct: 311 ELDKVIERLRVPSEYAASLTGGSFDEADMLQNIEACEWLAK---ALRGLEVPNLDPIYAN 367
Query: 84 QRLIGDYFANEPTYDDAMFRRRYRMQKHLFLRIVDDLSSSDNYFTQRVDAANKQGISPLA 143
R + + A RR ++ F +VD + S +YF+QR
Sbjct: 368 MRAVKEKRAELEKLKATFVRRASEFLRNYFASLVDFMVSDKSYFSQRGQLKRPDHADLRY 427
Query: 144 KCTTAMRMLAY 154
KC T R+L +
Sbjct: 428 KCRTYARLLQH 438
>At1g36675 putative protein
Length = 268
Score = 31.6 bits (70), Expect = 0.30
Identities = 13/19 (68%), Positives = 16/19 (83%)
Query: 119 DLSSSDNYFTQRVDAANKQ 137
DLSSSDNYFT+R +A K+
Sbjct: 175 DLSSSDNYFTKRFEATKKE 193
>At5g04460 putative protein
Length = 831
Score = 30.4 bits (67), Expect = 0.67
Identities = 23/81 (28%), Positives = 38/81 (46%), Gaps = 11/81 (13%)
Query: 15 ISQISSNLLQISQKMDPSECPFDLAAYIQNSQ----------IEEAYVLNRFRER-RRKI 63
+S + S+ L+ SQ PS A+ N+Q +E+ +VLNR RER R ++
Sbjct: 119 VSMLDSSFLRESQSQSPSSRRQGAASERPNTQASGILQMWRELEDEHVLNRARERVRERL 178
Query: 64 REDTAPRSRKYLGRDHTAANQ 84
R+ + S L + +Q
Sbjct: 179 RQQRSVESNTNLSSSIASESQ 199
>At5g35580 serine/threonine protein kinase-like
Length = 464
Score = 28.9 bits (63), Expect = 1.9
Identities = 14/48 (29%), Positives = 24/48 (49%)
Query: 53 LNRFRERRRKIREDTAPRSRKYLGRDHTAANQRLIGDYFANEPTYDDA 100
L F + RR++++ T +K+ HTA N R+ N P ++A
Sbjct: 412 LQNFDKTRREVKDTTTDNHQKFRSPAHTARNHRITLRNGYNSPMRNEA 459
>At4g21130 hypothetical protein
Length = 537
Score = 28.9 bits (63), Expect = 1.9
Identities = 31/131 (23%), Positives = 57/131 (42%), Gaps = 18/131 (13%)
Query: 46 QIEEAYVLNRFRERRRKIREDTAPRSRKYLGRDHTAANQRLIGDYFANE---PTYDDAMF 102
++E+ + E+R+++ EDT R + R+H N+ D F + T
Sbjct: 58 EVEDEFAHETVGEKRKRLAEDTLNRIEEAKQREHEEDNEE--DDDFRDSLVAKTLMQEQL 115
Query: 103 RRRYRMQKHLFLRIVDDLSSSDNYFTQRVDAANKQGISPLAKCTTAMR---------MLA 153
+ R+++ LR V DL SSD + RV ++ ++ +A R +L
Sbjct: 116 EKSGRVRRANALR-VQDLQSSDKF---RVIVKHQHSVTGVALSDDDSRGFSVSKDGTILH 171
Query: 154 YGVAADAVDEY 164
+ V++ DEY
Sbjct: 172 WDVSSGKSDEY 182
>At3g63430 unknown protein
Length = 540
Score = 28.5 bits (62), Expect = 2.5
Identities = 18/57 (31%), Positives = 28/57 (48%)
Query: 106 YRMQKHLFLRIVDDLSSSDNYFTQRVDAANKQGISPLAKCTTAMRMLAYGVAADAVD 162
+R + L RI D S+ +VD N+QG + L +C + +A AA+A D
Sbjct: 277 WRRPEPLQTRIEDQSSTLAEEEMWKVDVYNRQGKTLLERCDKLLHSIAEMAAAEAGD 333
>At4g02750 hypothetical protein
Length = 781
Score = 27.7 bits (60), Expect = 4.3
Identities = 12/21 (57%), Positives = 18/21 (85%)
Query: 38 LAAYIQNSQIEEAYVLNRFRE 58
L+AY+QNS++EEA +L + RE
Sbjct: 195 LSAYVQNSKMEEACMLFKSRE 215
>At4g07620 putative athila transposon protein
Length = 683
Score = 27.3 bits (59), Expect = 5.7
Identities = 20/88 (22%), Positives = 39/88 (43%), Gaps = 2/88 (2%)
Query: 9 ISIDVQISQISSNLLQISQKMDPSECPFDLAAYIQNSQIEEAYVLNRFRERRRKIREDTA 68
++ D +I+++ + + ++ K+D S ++ QNS+I+ YV N+F
Sbjct: 305 MAFDKKIAELHNKIAELHNKIDWSYNDLNVKFEAQNSKIK--YVENQFASTSAPKHPQQL 362
Query: 69 PRSRKYLGRDHTAANQRLIGDYFANEPT 96
P +D+ AN I + PT
Sbjct: 363 PGKAVQNPKDYATANAITIHQEDESPPT 390
>At1g23940 gamma-adaptin, putative
Length = 1111
Score = 26.6 bits (57), Expect = 9.6
Identities = 16/59 (27%), Positives = 30/59 (50%), Gaps = 3/59 (5%)
Query: 2 YLLIWTHISIDVQISQISSNLLQISQKMDPSECPFDLAAYIQNSQIEEAYVLNRFRERR 60
+LL W + D+ Q LLQ ++ CP+ L A+ + ++ E +L+R E++
Sbjct: 300 HLLCWAYAGSDLVSYQ---RLLQKWERKTLPLCPWYLCAFCEPEKLGEEEILDRNGEKK 355
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.324 0.137 0.397
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,152,368
Number of Sequences: 26719
Number of extensions: 156251
Number of successful extensions: 533
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 520
Number of HSP's gapped (non-prelim): 18
length of query: 204
length of database: 11,318,596
effective HSP length: 95
effective length of query: 109
effective length of database: 8,780,291
effective search space: 957051719
effective search space used: 957051719
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0115.1