BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0114.8
(743 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g28320 unknown protein 610 e-175
At3g27925 DegP protease precursor, 5' partial 50 5e-06
At4g18365 protease HhoA like precursor 44 3e-04
At5g39830 DegP protease-like protein 41 0.003
At5g40560 putative protein 33 0.58
At5g27660 serine protease-like protein 33 0.58
At2g47940 DegP2 protease (DEGP2) 30 3.8
At5g16850 telomerase reverse transcriptase 30 4.9
At5g40200 putative serine protease (MSN9.10) 30 6.4
At5g52860 ABC transporter-like protein 29 8.4
At1g65640 serine protease DO, putative 29 8.4
>At1g28320 unknown protein
Length = 709
Score = 610 bits (1573), Expect = e-175
Identities = 365/756 (48%), Positives = 463/756 (60%), Gaps = 68/756 (8%)
Query: 5 ETVNFARNFAVMVRVRGPCCVIMGQLTLQFFSGPERDEDEKTRFSSISVRIPICNLIQLV 64
+ V+F+RNFAV+V+V GP P+ + K F
Sbjct: 5 KVVSFSRNFAVLVKVEGP--------------DPKGLKMRKHAFHQYH------------ 38
Query: 65 SSGETTLSASGMLVPHTL-CDPQVARRLYGDNSEGRVMVVTVASVVEPFLSPQQRDNIPQ 123
SG TLSASG+L+P + +VA ++ + + +V+TVASVVEPFL+ R +
Sbjct: 39 -SGNATLSASGILLPRDIFLSGEVAAKVLFEAGQDMALVLTVASVVEPFLTLGHRTSSSI 97
Query: 124 FQGRPDLISGVRIDIMTEETNEKSDHGLTPGWHGAQLLSLFDIPASALGVQSLIEASLGF 183
Q LI G I+IM E + P W AQLLSL D+P S+ +QSLIEAS G
Sbjct: 98 SQDPVKLIPGAMIEIMVE--GQLKSEKEAPFWVPAQLLSLVDVPVSSAALQSLIEASSGS 155
Query: 184 SEHEWEIGWSLASSSNDSKPSRDFLQ-TQGRLAVGGSGSASLLCKSLTRMAILSVSLSLK 242
+ W+IGWSL S++N S+PS + ++ + + +A+ + KS TRMAIL V LSL
Sbjct: 156 KDSGWDIGWSLVSAANGSQPSINIEHYSKPLMQLDEPHNANFMAKSATRMAILGVPLSLL 215
Query: 243 DSLDSSVCSMIKRGDFLLAIGSPFGVLSPTHFF---------NSYPPNSSDGSLLMADIR 293
+ S +GD L+A+GSPFG+LSP +FF NSYP S SL++AD+R
Sbjct: 216 GQPSMNFASSSSKGDTLVALGSPFGILSPVNFFNSVSTGSIANSYPSGSLKKSLMIADVR 275
Query: 294 TLPGMEGSPVFSEHACLTGVLIRPLRQKTSGVETQLVIPWEAIVTASSGLLRK----WGK 349
LPGMEG+PVF+++ L G+LIRPLRQK SGVE QLV+PW AI TA S LL + GK
Sbjct: 276 CLPGMEGAPVFAKNGHLIGILIRPLRQKNSGVEIQLVVPWGAITTACSHLLLEEPSVEGK 335
Query: 350 GPFVGTYKLDVPASNTHEQLNLGSSSLLPIEKAMASVCLVTIGDGVWASGILLNSQGLVL 409
G+ L V + + + + IEKAM SVCL+T+ DGVWASGI+LN GL+L
Sbjct: 336 ASQWGSEVLSVKS-------DASIPAQVAIEKAMESVCLITVNDGVWASGIILNEHGLIL 388
Query: 410 TNAHLLEPWRFGKTHVSGGGNGTNSKTLPFMLEGTTSLGNKIESDQISQTLHSKVP-VHY 468
TNAHLLEPWR+GK GG G K E +S G+K +Q SQTL K P HY
Sbjct: 389 TNAHLLEPWRYGK----GGVYGEGFKPYVLGAEEFSSTGSKF-WEQKSQTLPRKAPRNHY 443
Query: 469 PFAADEHGGYKLNPSYDNHRNIRIRLDHIKPWVWCDAKVVYICKGPWDVALLQIESIPDN 528
+ YK N HR+IR+RL H+ W WC A VVYICK D+ALLQ+E +P
Sbjct: 444 SSVGENIREYKHNFLQTGHRDIRVRLCHLDSWTWCPANVVYICKEQLDIALLQLEYVPGK 503
Query: 529 LVPMVMNFSRPSTGSKAYVIGHGLFGPKCGFFPSVCSGVIAKVVEAKTTQSYQSIQAEHM 588
L P+ NFS P G+ A+V+GHGLFGP+CG PS+CSGV+AKVV AK + QSI E
Sbjct: 504 LQPITANFSSPPLGTTAHVVGHGLFGPRCGLSPSICSGVVAKVVHAKRRLNTQSISQE-- 561
Query: 589 HTNGNFPAMLETTAAVHPGASGGAVINSDGHMIGLVTSNARHSGGAVIPHLNFSIPSAAL 648
FPAMLETTAAVHPG SGGAV+NS GHMIGLVTSNARH G VIPHLNFSIP A L
Sbjct: 562 --VAEFPAMLETTAAVHPGGSGGAVLNSSGHMIGLVTSNARHGAGTVIPHLNFSIPCAVL 619
Query: 649 APIFKFAEDMKDLSLLQILDEPNEYLSSIWALMRPSYPKL-HSVPGSPQSLVDKKSKEEK 707
APIFKFAEDM++ ++LQ LD+P+E LSSIWALM PK S+P P+ L D +K+ K
Sbjct: 620 APIFKFAEDMQNTTILQTLDQPSEELSSIWALMPSLSPKTEQSLPNLPKLLKDGNNKQTK 679
Query: 708 GSRFAKFIAERKDIFNDSSHIGKSGVVPKEVIPSKL 743
GS+FAKFIAE +D+F K + ++VIPSKL
Sbjct: 680 GSQFAKFIAETQDMF------VKPTKLSRDVIPSKL 709
>At3g27925 DegP protease precursor, 5' partial
Length = 386
Score = 50.1 bits (118), Expect = 5e-06
Identities = 44/142 (30%), Positives = 71/142 (49%), Gaps = 19/142 (13%)
Query: 504 DAKVVYICKGPWDVALLQIESIPDNLVPMVMNFSRPS-TGSKAYVIGHGLFGPKCGFFPS 562
DAKVV + DVA+L+I++ + L P+ + S G K + IG+ FG
Sbjct: 139 DAKVVGFDQDK-DVAVLRIDAPKNKLRPIPVGVSADLLVGQKVFAIGNP-FGLDHTLTTG 196
Query: 563 VCSGVIAKVVEAKTTQSYQSIQAEHMHTNGNFPAMLETTAAVHPGASGGAVINSDGHMIG 622
V SG+ ++ A T + Q + ++T AA++PG SGG +++S G +IG
Sbjct: 197 VISGLRREISSAATGRPIQDV--------------IQTDAAINPGNSGGPLLDSSGTLIG 242
Query: 623 LVTSNARHSGGAVIPHLNFSIP 644
+ T+ SG + + FSIP
Sbjct: 243 INTAIYSPSGAS--SGVGFSIP 262
>At4g18365 protease HhoA like precursor
Length = 323
Score = 44.3 bits (103), Expect = 3e-04
Identities = 34/130 (26%), Positives = 63/130 (48%), Gaps = 17/130 (13%)
Query: 516 DVALLQIESIPDNLVPMVMNFSRP-STGSKAYVIGHGLFGPKCGFFPSVCSGVIAKVVEA 574
D+A+L+IE+ L P+V+ S G + IG+ G+ ++ GV++ +
Sbjct: 188 DLAVLKIETEGRELNPVVLGTSNDLRVGQSCFAIGNPY-----GYENTLTIGVVSGLGRE 242
Query: 575 KTTQSYQSIQAEHMHTNGNFPAMLETTAAVHPGASGGAVINSDGHMIGLVTSNARHSGGA 634
+ + +SI ++T A ++ G SGG +++S GH IG+ T+ G
Sbjct: 243 IPSPNGKSISEA-----------IQTDADINSGNSGGPLLDSYGHTIGVNTATFTRKGSG 291
Query: 635 VIPHLNFSIP 644
+ +NF+IP
Sbjct: 292 MSSGVNFAIP 301
>At5g39830 DegP protease-like protein
Length = 448
Score = 40.8 bits (94), Expect = 0.003
Identities = 61/289 (21%), Positives = 117/289 (40%), Gaps = 83/289 (28%)
Query: 364 NTHEQLNLGSSSLLPIEKAMASVCLVTIGDGVWASGILLNSQGLVLTNAHLLEPWRFGKT 423
NT+ +N+ +L P K M V + G+G SG++ + QG ++TN H++
Sbjct: 126 NTYSVVNIFDVTLRPQLK-MTGVVEIPEGNG---SGVVWDGQGYIVTNYHVI-------- 173
Query: 424 HVSGGGNGTNSKTLPFMLEGTTSLGNKIESDQISQTLHSKVPVHYPFAADEHGGYKLNPS 483
GN + P + G ++ + SD + + K+
Sbjct: 174 -----GNALSRNPSPGDVVGRVNI---LASDGVQKNFEGKL------------------- 206
Query: 484 YDNHRNIRIRLDHIKPWVWCDAKVVYICKGPWDVALLQIESIPDNLVPMVMNFSRP-STG 542
+ D K D+A+L++++ L P+ + S G
Sbjct: 207 --------VGADRAK-----------------DLAVLKVDAPETLLKPIKVGQSNSLKVG 241
Query: 543 SKAYVIGHGLFGPKCGFFPSVCSGVIAKVVEAKTTQSYQSIQAEHMHTNGNFPAMLETTA 602
+ IG+ GF ++ GVI+ + +Q+ +I ++T A
Sbjct: 242 QQCLAIGNPF-----GFDHTLTVGVISGLNRDIFSQTGVTIGGG-----------IQTDA 285
Query: 603 AVHPGASGGAVINSDGHMIGLVTSNARHSGGAVIPHLNFSIPSAALAPI 651
A++PG SGG +++S G++IG+ T+ +G + + F+IPS+ + I
Sbjct: 286 AINPGNSGGPLLDSKGNLIGINTAIFTQTGTSA--GVGFAIPSSTVLKI 332
>At5g40560 putative protein
Length = 410
Score = 33.1 bits (74), Expect = 0.58
Identities = 36/126 (28%), Positives = 59/126 (46%), Gaps = 22/126 (17%)
Query: 516 DVALLQIESIP--DNLVPMVMNFSRPSTGSKAYVIGHGLFGPKCGFFPSVCSGVIAKVVE 573
D+A+L IES +++ P+ + P VIG+ P G SV GV++++
Sbjct: 38 DLAILVIESKEFWEDMNPLELG-DMPFLQESVNVIGY----PTGGENISVTKGVVSRIES 92
Query: 574 AKTTQSYQSIQAEHMHTNGNFPAMLETTAAVHPGASGGAVINSDGHMIGLVTSNARHSG- 632
++ H N PA ++T AA++PG SGG V + ++G+ HS
Sbjct: 93 M-----------DYAHGAINLPA-IQTDAAMNPGNSGGPVCIGN-KVVGVAFQTLGHSNN 139
Query: 633 -GAVIP 637
G +IP
Sbjct: 140 IGCLIP 145
>At5g27660 serine protease-like protein
Length = 459
Score = 33.1 bits (74), Expect = 0.58
Identities = 18/62 (29%), Positives = 32/62 (51%), Gaps = 5/62 (8%)
Query: 592 GNFPAMLETTAAVHPGASGGAVINSDGHMIGLVTSNARHSGGAVIPHLNFSIPSAALAPI 651
G L+T +++ G SGG ++N DG +IG+ + G L FS+P +++ I
Sbjct: 294 GKHREYLQTDCSINAGNSGGPLVNLDGEVIGVNIMKVLAADG-----LGFSVPIDSVSKI 348
Query: 652 FK 653
+
Sbjct: 349 IE 350
>At2g47940 DegP2 protease (DEGP2)
Length = 607
Score = 30.4 bits (67), Expect = 3.8
Identities = 32/128 (25%), Positives = 49/128 (38%), Gaps = 23/128 (17%)
Query: 516 DVALLQIESIP--DNLVPMVMNFSRPSTGSKAYVIGHGLFGPKCGFFPSVCSGVIAKVVE 573
D+ALL +ES P+ + P V+G+ L G SV GV++++
Sbjct: 190 DIALLSVESEDFWKGAEPLRLGHL-PRLQDSVTVVGYPLGGDTI----SVTKGVVSRIEV 244
Query: 574 AKTTQSYQSIQAEHMHTNGNFPAMLETTAAVHPGASGGAVINSDGHMIGLV----TSNAR 629
+ ++ AA++PG SGG N G IG+ S
Sbjct: 245 TSYAHGSSDLLG------------IQIDAAINPGNSGGPAFNDQGECIGVAFQVYRSEET 292
Query: 630 HSGGAVIP 637
+ G VIP
Sbjct: 293 ENIGYVIP 300
>At5g16850 telomerase reverse transcriptase
Length = 1123
Score = 30.0 bits (66), Expect = 4.9
Identities = 17/61 (27%), Positives = 28/61 (45%), Gaps = 3/61 (4%)
Query: 235 LSVSLSLKDSLDSSV--CSMIKRG-DFLLAIGSPFGVLSPTHFFNSYPPNSSDGSLLMAD 291
+++ + + SL V +KR +F + + V+ P H + PN SD LLM
Sbjct: 284 MNLHIGINGSLTDFVKQAKQVKRNKNFKFGLSETYSVIPPNHILKTLRPNCSDSKLLMNH 343
Query: 292 I 292
I
Sbjct: 344 I 344
>At5g40200 putative serine protease (MSN9.10)
Length = 592
Score = 29.6 bits (65), Expect = 6.4
Identities = 15/44 (34%), Positives = 22/44 (49%), Gaps = 4/44 (9%)
Query: 598 LETTAAVHPGASGGAVINSDGHMIGLVTSNARHSG----GAVIP 637
L+ AA++ G SGG N G +G+ + +H G VIP
Sbjct: 267 LQIDAAINSGNSGGPAFNDKGKCVGIAFQSLKHEDAENIGYVIP 310
>At5g52860 ABC transporter-like protein
Length = 589
Score = 29.3 bits (64), Expect = 8.4
Identities = 16/49 (32%), Positives = 25/49 (50%)
Query: 352 FVGTYKLDVPASNTHEQLNLGSSSLLPIEKAMASVCLVTIGDGVWASGI 400
F YK + A +E L S L+ +E+A +C+VT GD + G+
Sbjct: 505 FFSMYKYALDALLINEYSCLASKCLVWLEEAQTKICMVTGGDVLKKKGL 553
>At1g65640 serine protease DO, putative
Length = 518
Score = 29.3 bits (64), Expect = 8.4
Identities = 25/101 (24%), Positives = 46/101 (44%), Gaps = 19/101 (18%)
Query: 516 DVALLQI--ESIPDNLVPMVMNFSRPSTGSKAYVIGHGLFGPKCGFFPSVCSGVIAKVVE 573
D+A+L+I E ++++P+ + PS V G+ P G S+ G +++V
Sbjct: 147 DLAILEIDNEEFWEDMIPLELG-EIPSLDESVAVFGY----PTGGDSVSITKGYVSRVEY 201
Query: 574 AKTTQSYQSIQAEHMHTNGNFPAMLETTAAVHPGASGGAVI 614
+ ++ A ++T AA++PG SGG I
Sbjct: 202 TRYAHGGTTLLA------------IQTDAAINPGNSGGPAI 230
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.317 0.135 0.403
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,188,589
Number of Sequences: 26719
Number of extensions: 756254
Number of successful extensions: 1782
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 1757
Number of HSP's gapped (non-prelim): 22
length of query: 743
length of database: 11,318,596
effective HSP length: 107
effective length of query: 636
effective length of database: 8,459,663
effective search space: 5380345668
effective search space used: 5380345668
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 64 (29.3 bits)
Lotus: description of TM0114.8