BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0106.9
         (458 letters)

Database: ara_mips 
           26,719 sequences; 11,318,596 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

At2g03820 putative nonsense-mediated mRNA decay protein               577  e-165
At2g07650 putative non-LTR retrolelement reverse transcriptase         30  2.8
At5g18650 unknown protein                                              29  4.7
At5g17020 Exportin1 (XPO1) protein                                     29  4.7
At3g03110 exportin1 (XPO1) like protein                                29  4.7
At4g27180 heavy chain polypeptide of kinesin-like protein (katB)       28  8.1
At1g69420 hypothetical protein                                         28  8.1

>At2g03820 putative nonsense-mediated mRNA decay protein
          Length = 516

 Score =  577 bits (1487), Expect = e-165
 Identities = 284/450 (63%), Positives = 348/450 (77%), Gaps = 11/450 (2%)

Query: 12  EAGMFSVPQTICSVLCCMCGIPMQPNAANMCVKCLSSQEDITEGLQKHINIFHCPQCESY 71
           E+GMF+V QTI SVLCC CG+PM PNAANMCV CL S+ DITEGLQK I IF+CP+C  Y
Sbjct: 6   ESGMFNVQQTIGSVLCCKCGVPMAPNAANMCVNCLRSEVDITEGLQKSIQIFYCPECTCY 65

Query: 72  FQHPKTWIKHLKPESDELLKFCLKMMKKTNKVRMVDARFIWTEPHSKRIKINVKVQKEVL 131
            Q PKTWIK  + ES ELL FC+K +K  NKV++ +A F+WTEPHSKRIK+ + VQ EVL
Sbjct: 66  LQPPKTWIK-CQWESKELLTFCIKRLKNLNKVKLKNAEFVWTEPHSKRIKVKLTVQAEVL 124

Query: 132 NGVILEQSYLVEYVQLEHLCDSCTRVAANPDQWVAVVQLRQHVSHRRTFFYLEQLILKHG 191
           NG +LEQSY VEY   ++LC+SC+R  ANPDQWVA +QLRQHVSHRRTFFYLEQLIL+H 
Sbjct: 125 NGAVLEQSYPVEYTVRDNLCESCSRFQANPDQWVASIQLRQHVSHRRTFFYLEQLILRHD 184

Query: 192 AAARAIRIKQMHEGIDFYFSNEDHGEKFVQFIGKVAPIRSKGYNKQLVSHDTKSNNYNYR 251
           AA+RAIRI+Q+ +GIDF+F N+ H   FV+F+ KV PI  +  ++QLVSHD KS+ YNY+
Sbjct: 185 AASRAIRIQQVDQGIDFFFGNKSHANSFVEFLRKVVPIEYR-QDQQLVSHDVKSSLYNYK 243

Query: 252 YTFSVEISPICREDLICLPHHVALSLGNLGPLVICTKVTNSIVLLDPFTLRHCFLDASQY 311
           YT+SV+I P+CREDL+CLP  VA  LGNLGPLV+CTKV+++I LLDP TLR  FLDA QY
Sbjct: 244 YTYSVKICPVCREDLVCLPSKVASGLGNLGPLVVCTKVSDNITLLDPRTLRCAFLDARQY 303

Query: 312 WRASFKSLLTSRQLVEYVVLDVEEVVPSEVVTVGGTKYVLAVAQVARVSDFGKNDTIFSI 371
           WR+ F+S LTSRQLV+Y V DVE   P    TVGG KY L+  Q+AR SD GK   +F +
Sbjct: 304 WRSGFRSALTSRQLVKYFVFDVEP--PVGEATVGGQKYALSYVQIARESDIGK---MFYV 358

Query: 372 KTHLGHLLNPGDYALGYDLYGANCNDIELDKYK---GGDLPHAILVKKSYVEKRLKKRGK 428
           +THLGH+L PGD ALGYD+YGAN ND E++KY+      LP AIL+KK Y E+R +K+ K
Sbjct: 359 QTHLGHILKPGDQALGYDIYGANVNDNEMEKYRLSVKNGLPEAILIKKCYEEQRERKQKK 418

Query: 429 PRSWKLKSLNMEIDD-KGRTDPDKMSSEYE 457
            R+WKLKSL ME+DD +GR DP+K   EYE
Sbjct: 419 SRNWKLKSLPMEMDDSRGRVDPEKTDKEYE 448


>At2g07650 putative non-LTR retrolelement reverse transcriptase
          Length = 732

 Score = 30.0 bits (66), Expect = 2.8
 Identities = 18/87 (20%), Positives = 38/87 (42%), Gaps = 12/87 (13%)

Query: 37  NAANMCVKCLSSQEDITEGLQKHINIFHCPQCESYFQHPKTWIKHLKPESDELLKFCLKM 96
           +   +C  C  ++E I   L+       CP  E        W++ +   ++E+    LK+
Sbjct: 601 STTTICQVCKGAKESIIHVLRD------CPAMEGI------WLRLISDLANEVSAAHLKL 648

Query: 97  MKKTNKVRMVDARFIWTEPHSKRIKIN 123
              T ++  V+ +  W +P+   +K+N
Sbjct: 649 SGNTKEIVQVERQVSWRKPNEGWVKLN 675


>At5g18650 unknown protein
          Length = 267

 Score = 29.3 bits (64), Expect = 4.7
 Identities = 22/76 (28%), Positives = 30/76 (38%), Gaps = 25/76 (32%)

Query: 18  VPQTICSV---------LCCMCGIPMQPNAANMCVKCLSSQEDITEGLQKHI-------- 60
           V Q ICSV         +C  CG+ M     ++C+      +D TE  Q H         
Sbjct: 63  VKQVICSVCDTEQPAAQVCSNCGVNMGEYFCSICI----FYDDDTEKQQFHCDDCGICRV 118

Query: 61  ----NIFHCPQCESYF 72
               N FHC +C S +
Sbjct: 119 GGRENFFHCKKCGSCY 134


>At5g17020 Exportin1 (XPO1) protein
          Length = 1075

 Score = 29.3 bits (64), Expect = 4.7
 Identities = 28/95 (29%), Positives = 43/95 (44%), Gaps = 19/95 (20%)

Query: 159 ANPDQWVAVVQLRQHVSHRRTFFYLEQL---ILKHGAAARAIRIKQMHEGIDFYFSNEDH 215
           ANPD W+ VV + Q+ +   T F+  Q+   ++K+   A  +   +  +G+  Y S    
Sbjct: 46  ANPDMWLQVVHILQNTNSLDTKFFALQVLEGVIKYRWNALPV---EQRDGMKNYIS---- 98

Query: 216 GEKFVQFIGKVAPIRSKG--YNK------QLVSHD 242
            E  VQ     A  RS+    NK      Q+V HD
Sbjct: 99  -EVIVQLSSNEASFRSERLYVNKLNVILVQIVKHD 132


>At3g03110 exportin1 (XPO1) like protein
          Length = 1022

 Score = 29.3 bits (64), Expect = 4.7
 Identities = 12/29 (41%), Positives = 18/29 (61%)

Query: 159 ANPDQWVAVVQLRQHVSHRRTFFYLEQLI 187
           ANPD W+ VV + Q+ S   T F+  Q++
Sbjct: 46  ANPDTWLQVVHILQNTSSTHTKFFALQVL 74


>At4g27180 heavy chain polypeptide of kinesin-like protein (katB)
          Length = 745

 Score = 28.5 bits (62), Expect = 8.1
 Identities = 23/94 (24%), Positives = 43/94 (45%), Gaps = 7/94 (7%)

Query: 67  QCESYFQHPKTWIKHLKPESDELLKFCLKMMKKTNKVRMVDARFIWTEPHSKRIKINVKV 126
           +CE+   + K     ++   +  L +  +  K  N + M        E H   +++N+KV
Sbjct: 58  RCENTMDYVKRLRLCIRWFQELELDYAFEQEKLKNAMEM-------NEKHCADLEVNLKV 110

Query: 127 QKEVLNGVILEQSYLVEYVQLEHLCDSCTRVAAN 160
           ++E LN VI E       VQ++   +   ++AAN
Sbjct: 111 KEEELNMVIDELRKNFASVQVQLAKEQTEKLAAN 144


>At1g69420 hypothetical protein
          Length = 596

 Score = 28.5 bits (62), Expect = 8.1
 Identities = 17/42 (40%), Positives = 22/42 (51%), Gaps = 3/42 (7%)

Query: 415 KKSYVEKRLKKRGKP---RSWKLKSLNMEIDDKGRTDPDKMS 453
           KKS VE+R+KK+ +P     W L  LN E   K   +  K S
Sbjct: 367 KKSVVEERVKKKPQPVKISPWTLARLNAEEVSKAAAEARKKS 408


  Database: ara_mips
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 2,978,382
  Number of sequences in database:  6832
  
  Database: /data/blast2/ara_mips_chr2
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 1,737,135
  Number of sequences in database:  4184
  
  Database: /data/blast2/ara_mips_chr3
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 2,236,886
  Number of sequences in database:  5377
  
  Database: /data/blast2/ara_mips_chr4
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 1,748,816
  Number of sequences in database:  4030
  
  Database: /data/blast2/ara_mips_chr5
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 2,569,679
  Number of sequences in database:  6098
  
  Database: /data/blast2/ara_mips_chl
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 25,951
  Number of sequences in database:  85
  
  Database: /data/blast2/ara_mips_mit
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 21,747
  Number of sequences in database:  113
  
Lambda     K      H
   0.322    0.137    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,663,524
Number of Sequences: 26719
Number of extensions: 459508
Number of successful extensions: 1132
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 1124
Number of HSP's gapped (non-prelim): 7
length of query: 458
length of database: 11,318,596
effective HSP length: 103
effective length of query: 355
effective length of database: 8,566,539
effective search space: 3041121345
effective search space used: 3041121345
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 62 (28.5 bits)