BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0103.4
(378 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g63460 putative protein 36 0.030
At4g37450 arabinogalactan protein AGP18 36 0.040
At2g39580 unknown protein 35 0.088
At5g27870 pectin methyl-esterase - like protein 34 0.15
At2g30340 unknown protein 33 0.34
At2g35230 unknown protein 32 0.44
At1g54970 hypothetical protein 32 0.44
At1g41900 hypothetical protein 32 0.44
At5g56890 unknown protein 32 0.57
At1g49270 hypothetical protein 31 0.98
At4g02960 putative polyprotein of LTR transposon 30 1.7
At3g27210 unknown protein 30 2.2
At3g24400 protein kinase, putative 29 3.7
At1g76660 unknown protein 29 3.7
At5g19910 SOH1 - like protein 29 4.8
At5g06640 putative protein 29 4.8
At1g79480 hypothetical protein 29 4.8
At1g68690 protein kinase, putative 29 4.8
At1g55130 putative endomembrane protein 70 29 4.8
At4g34440 serine/threonine protein kinase - like 28 6.3
>At3g63460 putative protein
Length = 1097
Score = 36.2 bits (82), Expect = 0.030
Identities = 35/184 (19%), Positives = 73/184 (39%), Gaps = 28/184 (15%)
Query: 163 VMIRCAGKTRSEMSITEAFDEDPDFMADRGRVDNSSVGYGWLESFELLVTVIALIRLTFG 222
V+ G + S+ + F+ + +A +G LL T + +++
Sbjct: 716 VLALATGNKKFSASLCKLFESYAEILASQG----------------LLTTAMKYLKVLDS 759
Query: 223 TGVRPPISQHNKKVSRLGSNPMNKLDSPPKTDSIITPNTRLKIHVPPRPHPALASPSLAS 282
G+ P +S ++S L + P+T++ + NT+ + +P P A P++ +
Sbjct: 760 GGLSPELSILRDRIS---------LSAEPETNTTASGNTQPQSTMPYNQEPTQAQPNVLA 810
Query: 283 STLADPRSGPSVTQSILPTV--PAMENCKIAM-HRNPPKSQVNYTTILQGNLLVNQSNHL 339
+ + P +P V P M+ + M H+ P Q ++T N +
Sbjct: 811 NPYDNQYQQPYTDSYYVPQVSHPPMQQPTMFMPHQAQPAPQPSFTPAPTSNAQPSMRTTF 870
Query: 340 LPAT 343
+P+T
Sbjct: 871 VPST 874
Score = 28.1 bits (61), Expect = 8.3
Identities = 23/91 (25%), Positives = 39/91 (42%), Gaps = 12/91 (13%)
Query: 241 SNPMNKLDSPPKTDSIITPNTRLKIHVPPR------PHPALASPSLASSTLADPRSGPSV 294
+NP + P TDS P H P + PH A +P + + + PS+
Sbjct: 810 ANPYDNQYQQPYTDSYYVPQVS---HPPMQQPTMFMPHQAQPAPQPSFTPAPTSNAQPSM 866
Query: 295 TQSILP-TVPAMENCKIAMHRNPPKSQVNYT 324
+ +P T PA++N ++ P S ++T
Sbjct: 867 RTTFVPSTPPALKNAD--QYQQPTMSSHSFT 895
>At4g37450 arabinogalactan protein AGP18
Length = 209
Score = 35.8 bits (81), Expect = 0.040
Identities = 29/102 (28%), Positives = 45/102 (43%), Gaps = 16/102 (15%)
Query: 206 SFELLVTVIALIRLTFGTGVRPPISQHNKKVSRLGSNPMNKLDSPPKTDSIITPNTRLKI 265
+F L VT+I ++ G G + PIS K + P SP K+ ++ +P T
Sbjct: 4 NFLLTVTLICIV--VAGVGGQSPISSPTKSPT----TPSAPTTSPTKSPAVTSPTTA--- 54
Query: 266 HVPPRPHPALASPSLASSTLADPRSGPSVTQSILPTVPAMEN 307
PA + ASS + P+S V++S P P E+
Sbjct: 55 -------PAKTPTASASSPVESPKSPAPVSESSPPPTPVPES 89
>At2g39580 unknown protein
Length = 1567
Score = 34.7 bits (78), Expect = 0.088
Identities = 30/130 (23%), Positives = 59/130 (45%), Gaps = 12/130 (9%)
Query: 230 SQHNKKVSRLGSNPMNKLDSPPKTDSIITPNTR-LKIHVPPRPHPALASPSLA------- 281
S+ K ++ + +N +P + SII+ +TR L+ + PP+ ++ +
Sbjct: 226 SELKLKAAQPKKDAVNPKITPARRVSIISDDTRHLEPNEPPKKRLKVSGIDTSQPVIDYR 285
Query: 282 -SSTLADPRSGPSVTQSILPTVPAMENCKIAMHRNPPKSQVNYTTILQGNLLVNQSNHLL 340
+++ A P + P + +S+LP V A +CK H ++ I Q + N S+ +L
Sbjct: 286 VAASAAAPMNAPDIRKSLLPGVNANSSCK---HLGSKSDEIVPPVIPQHTVEGNTSSSVL 342
Query: 341 PATNNYSQHY 350
+ HY
Sbjct: 343 QKSTGKVNHY 352
>At5g27870 pectin methyl-esterase - like protein
Length = 732
Score = 33.9 bits (76), Expect = 0.15
Identities = 25/68 (36%), Positives = 34/68 (49%), Gaps = 8/68 (11%)
Query: 236 VSRLGSNPMNKLDSPPKT-DSIITPNTRLKIHVPPRPHPALASPSLASSTLADPRSGPSV 294
VS S P L SP T S+++P+T PP H L SPS S++ P + PS
Sbjct: 637 VSPSTSPPAGHLGSPSDTPSSLVSPSTS-----PPAGH--LGSPSDTPSSVVTPSASPST 689
Query: 295 TQSILPTV 302
+ S P+V
Sbjct: 690 SPSASPSV 697
Score = 28.9 bits (63), Expect = 4.8
Identities = 26/76 (34%), Positives = 36/76 (47%), Gaps = 10/76 (13%)
Query: 228 PISQHNKKVSRLGSNPMNKLDSPPKT-DSIITPNTRLKIHVPPRPHPALASPSLASSTLA 286
P + VS S P L SP T S++TP+ P P+ ASPS++ S A
Sbjct: 651 PSDTPSSLVSPSTSPPAGHLGSPSDTPSSVVTPSAS------PSTSPS-ASPSVSPS--A 701
Query: 287 DPRSGPSVTQSILPTV 302
P + PS + S P+V
Sbjct: 702 FPSASPSASPSASPSV 717
>At2g30340 unknown protein
Length = 268
Score = 32.7 bits (73), Expect = 0.34
Identities = 29/116 (25%), Positives = 46/116 (39%), Gaps = 8/116 (6%)
Query: 223 TGVRPPISQHNKKVSRLGSNPMNKLDSPPKTDSIITPNTRLKIHV----PPRPHPALASP 278
T VR I +H + + ++ N +S T S+ L + PP P P P
Sbjct: 146 TTVRTEILRHKYQEATTITSLQNNFNSTTTTSSVSCDQHALASAILLPPPPPPPPTPRPP 205
Query: 279 SLASSTLADPR----SGPSVTQSILPTVPAMENCKIAMHRNPPKSQVNYTTILQGN 330
L SS A P S PS + + + + + +M+ PP S Y+ L +
Sbjct: 206 RLLSSQPAPPPTPPVSLPSPSMVVSSSSSSNSSATNSMYNPPPSSTAGYSNSLSSD 261
>At2g35230 unknown protein
Length = 402
Score = 32.3 bits (72), Expect = 0.44
Identities = 18/53 (33%), Positives = 28/53 (51%), Gaps = 1/53 (1%)
Query: 240 GSNPMNKLDSPPKTDSIITPNTRLKIHVPPRPHPALASPSLASSTLADPRSGP 292
GS L PP+ +S+ NTRL+ + P P L P++ ++A P+S P
Sbjct: 64 GSPSRESLPRPPQNNSLRPQNTRLQ-RIRPSPLTQLNRPAVPLPSMAPPQSHP 115
>At1g54970 hypothetical protein
Length = 335
Score = 32.3 bits (72), Expect = 0.44
Identities = 21/66 (31%), Positives = 29/66 (43%), Gaps = 4/66 (6%)
Query: 241 SNPMNKLDSPPK--TDSIITPNTRLKIHVPPRPHPALASPSLASSTLADPRSGPSVTQSI 298
++P+NK PP T + P ++ PP P L+ P TL P P V
Sbjct: 34 TSPVNKPTLPPPVYTPPVHKPTLPPPVYTPPVHKPTLSPPVYTKPTLPPPAYTPPVYNK- 92
Query: 299 LPTVPA 304
PT+PA
Sbjct: 93 -PTLPA 97
>At1g41900 hypothetical protein
Length = 442
Score = 32.3 bits (72), Expect = 0.44
Identities = 18/66 (27%), Positives = 32/66 (48%), Gaps = 6/66 (9%)
Query: 233 NKKVSRLGSNPMNKLDSPP------KTDSIITPNTRLKIHVPPRPHPALASPSLASSTLA 286
N +V+++ + + D P +T + + R+ R PA A+P A+S +
Sbjct: 286 NNQVAQVSGPSVVRSDGAPSSPKRARTQPEVVSDQRVASPREERMEPATATPEAAASAIG 345
Query: 287 DPRSGP 292
DPR+GP
Sbjct: 346 DPRAGP 351
>At5g56890 unknown protein
Length = 1113
Score = 32.0 bits (71), Expect = 0.57
Identities = 26/80 (32%), Positives = 34/80 (42%), Gaps = 6/80 (7%)
Query: 243 PMNKLDSPPKTDSIITPNTRLKIHVPP---RPHPALASPSLASSTLADPRSG--PSVTQS 297
P+ + PP T S + P H PP + +ASP S +A P P +T S
Sbjct: 163 PIPSVALPPPTPSNVPPRNASNNHKPPPIEKSIAPVASPPTISIDIAPPVHPVIPKLTPS 222
Query: 298 ILPTVPAMENCKIAMHRNPP 317
P VP K + RNPP
Sbjct: 223 SSP-VPTSTPTKGSPRRNPP 241
>At1g49270 hypothetical protein
Length = 699
Score = 31.2 bits (69), Expect = 0.98
Identities = 21/74 (28%), Positives = 30/74 (40%)
Query: 227 PPISQHNKKVSRLGSNPMNKLDSPPKTDSIITPNTRLKIHVPPRPHPALASPSLASSTLA 286
PP S + S S+ PP + I P + + PP P P +S +SS+
Sbjct: 22 PPSSNDQQTTSPPPSDNQETTSPPPPSSPDIAPPPQQQQESPPPPLPENSSDGSSSSSPP 81
Query: 287 DPRSGPSVTQSILP 300
P S +QS P
Sbjct: 82 PPSDSSSQSQSPPP 95
>At4g02960 putative polyprotein of LTR transposon
Length = 1456
Score = 30.4 bits (67), Expect = 1.7
Identities = 26/99 (26%), Positives = 39/99 (39%), Gaps = 13/99 (13%)
Query: 224 GVRPPISQHNKKVSRLGSNPMNKLDSPPKTDSIITPNTRLKIHVPPRPHPALASPSLASS 283
G +P H + S S +N + P + S +PN + P P + +PS + S
Sbjct: 817 GPQPTAQPHQTQNSNSNSPILNNPN--PNSPSPNSPNQNSPLPQSPISSPHIPTPSTSIS 874
Query: 284 TLADPRSGPSVTQSILPTVPAMENCKIAMHRNPPKSQVN 322
P S + T + P +PA PP QVN
Sbjct: 875 EPNSPSSSSTSTPPLPPVLPA-----------PPIIQVN 902
>At3g27210 unknown protein
Length = 234
Score = 30.0 bits (66), Expect = 2.2
Identities = 16/57 (28%), Positives = 33/57 (57%), Gaps = 2/57 (3%)
Query: 239 LGSNPMNKLD-SPPKTDSIITPNTRLKIHVPPRPHPALASPSLASSTLADPRSGPSV 294
+GS+ + L+ SP + DS++TP +++ ++ + ++ SPS+ S PR S+
Sbjct: 1 MGSSSSSSLNNSPIRADSMVTPESQMTVNDNKNDNVSILSPSVKKS-FESPRKSTSI 56
>At3g24400 protein kinase, putative
Length = 694
Score = 29.3 bits (64), Expect = 3.7
Identities = 24/69 (34%), Positives = 31/69 (44%), Gaps = 8/69 (11%)
Query: 249 SPPKTDSIITPNTRLKIHVPPRPHPALASPSLASSTLADPRSGPSVTQSILPTVPAMENC 308
SPP T S + P+ PP P P++ SP L T + P S P S P PA +
Sbjct: 116 SPPLTPSPLPPSPTTPS--PPPPSPSIPSPPL---TPSPPPSSPLRPSSPPPPSPATPS- 169
Query: 309 KIAMHRNPP 317
R+PP
Sbjct: 170 --TPPRSPP 176
>At1g76660 unknown protein
Length = 431
Score = 29.3 bits (64), Expect = 3.7
Identities = 15/42 (35%), Positives = 21/42 (49%)
Query: 276 ASPSLASSTLADPRSGPSVTQSILPTVPAMENCKIAMHRNPP 317
A+ + S LA P S S T S LP+ NC +++ N P
Sbjct: 60 AAGGINLSLLAPPSSPASFTNSALPSTTQSPNCYLSLAANSP 101
>At5g19910 SOH1 - like protein
Length = 196
Score = 28.9 bits (63), Expect = 4.8
Identities = 19/49 (38%), Positives = 25/49 (50%), Gaps = 5/49 (10%)
Query: 260 NTRLKIHVPPRP--HPALASPSLASSTLADPRSGPSVTQSILPTVPAME 306
N RLK H+ PRP P P +A ST P PS T ++ P + M+
Sbjct: 124 NNRLK-HILPRPLPEPVPPQPPVAPSTSLPP--APSATAALSPALSPMQ 169
>At5g06640 putative protein
Length = 689
Score = 28.9 bits (63), Expect = 4.8
Identities = 21/91 (23%), Positives = 33/91 (36%)
Query: 227 PPISQHNKKVSRLGSNPMNKLDSPPKTDSIITPNTRLKIHVPPRPHPALASPSLASSTLA 286
PP + KV+ P SPP +P K PP + + P + S +
Sbjct: 539 PPYYSPSPKVNYKSPPPPYVYSSPPPPYYSPSPKVNYKSPPPPYVYSSPPPPYYSPSPMV 598
Query: 287 DPRSGPSVTQSILPTVPAMENCKIAMHRNPP 317
D +S P P +P +++PP
Sbjct: 599 DYKSTPPPYVYSFPPLPYYSPSPKVDYKSPP 629
>At1g79480 hypothetical protein
Length = 356
Score = 28.9 bits (63), Expect = 4.8
Identities = 27/100 (27%), Positives = 43/100 (43%), Gaps = 8/100 (8%)
Query: 227 PPISQHNKKVSRLGSNPMNKLDSPPKTDSIITPNTRLKIHVP-PRPHPALASPSLASSTL 285
PP S N + NP +P DS PN+ V P P + ++P+ S+
Sbjct: 89 PPDSSSNPNSN---PNPPESSSNPNPPDSSSNPNSNPNPPVTVPNPPESSSNPNPPDSS- 144
Query: 286 ADPRSGPSVTQSIL---PTVPAMENCKIAMHRNPPKSQVN 322
++P S P+ +S P V + + + NPP+S N
Sbjct: 145 SNPNSNPNPPESSSNPNPPVTVPNPPESSSNPNPPESSSN 184
>At1g68690 protein kinase, putative
Length = 708
Score = 28.9 bits (63), Expect = 4.8
Identities = 24/90 (26%), Positives = 34/90 (37%), Gaps = 11/90 (12%)
Query: 223 TGVRPPISQHNKKVSRLGSNPMNKLDSP---PKTDSIITPNTRLKIHVPPRPHPALASPS 279
T V+PP+S S P+N SP P S + P+ PP P P SP
Sbjct: 4 TPVQPPVSNSPPVTSP--PPPLNNATSPATPPPVTSPLPPSAPPPNRAPPPPPPVTTSPP 61
Query: 280 LASSTL------ADPRSGPSVTQSILPTVP 303
++ P S Q ++P+ P
Sbjct: 62 PVANGAPPPPLPKPPESSSPPPQPVIPSPP 91
>At1g55130 putative endomembrane protein 70
Length = 637
Score = 28.9 bits (63), Expect = 4.8
Identities = 21/58 (36%), Positives = 30/58 (51%), Gaps = 8/58 (13%)
Query: 115 YIPSGTVYLYTFGYSISIY---LRVQYIPSGTVYLYTFGYSIYLRASVSRFVMIRCAG 169
Y+ SG+ LY F YS+ + L + + SG +Y FGY I + S S FV+ G
Sbjct: 567 YLTSGSSSLYLFLYSVFYFFTKLEISKLVSGVLY---FGYMIII--SYSFFVLTGSIG 619
>At4g34440 serine/threonine protein kinase - like
Length = 670
Score = 28.5 bits (62), Expect = 6.3
Identities = 22/86 (25%), Positives = 30/86 (34%)
Query: 237 SRLGSNPMNKLDSPPKTDSIITPNTRLKIHVPPRPHPALASPSLASSTLADPRSGPSVTQ 296
S S P PP + S P+ PP P SP + S+ + PS
Sbjct: 27 SNESSPPTPPSSPPPSSISAPPPDISASFSPPPAPPTQETSPPTSPSSSPPVVANPSPQT 86
Query: 297 SILPTVPAMENCKIAMHRNPPKSQVN 322
P+ PA E PP++ N
Sbjct: 87 PENPSPPAPEGSTPVTPPAPPQTPSN 112
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.321 0.138 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,821,539
Number of Sequences: 26719
Number of extensions: 406190
Number of successful extensions: 1349
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 29
Number of HSP's that attempted gapping in prelim test: 1276
Number of HSP's gapped (non-prelim): 80
length of query: 378
length of database: 11,318,596
effective HSP length: 101
effective length of query: 277
effective length of database: 8,619,977
effective search space: 2387733629
effective search space used: 2387733629
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0103.4