BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0100a.12
(405 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g64380 fructose-bisphosphatase-like protein 545 e-155
At3g54050 fructose-bisphosphatase precursor 266 2e-71
At1g43670 fructose 1,6-bisphosphatase, putative 243 1e-64
At3g55800 sedoheptulose-bisphosphatase precursor 69 6e-12
At5g35160 unknown protein 33 0.37
At5g35100 putative protein 32 0.48
At2g37240 unknown protein 32 0.82
At1g67035 putative protein 30 2.4
At5g60940 cleavage stimulation factor 50K chain 30 3.1
At3g19020 hypothetical protein 30 3.1
At1g01840 putative protein 30 3.1
At2g32710 cyclin-dependent kinase inhibitor (ACK2) 29 4.0
At2g26120 hypothetical protein 29 4.0
At2g11005 putative protein 29 4.0
At4g29240 extensin-like protein 29 5.3
At4g11660 heat shock transcription factor - like protein 29 5.3
At3g06130 unknown protein 29 5.3
At2g03300 putative disease resistance protein 29 5.3
At2g03030 putative disease resistance protein 29 5.3
At1g52070 unknown protein 29 5.3
>At5g64380 fructose-bisphosphatase-like protein
Length = 404
Score = 545 bits (1404), Expect = e-155
Identities = 278/372 (74%), Positives = 318/372 (84%), Gaps = 13/372 (3%)
Query: 37 LASISGSAKMRPLRALGGSSSSAGGDGDDGDGVVTLIEYLGK---EGIDLKDDLVVLLDH 93
+AS S +PL A+G + + GDD DG TLI++ G EG ++ +DLVVLL H
Sbjct: 43 MASRHQSTSFKPL-AVGRNLT-----GDDDDGYCTLIDFAGSGGGEGKNVGEDLVVLLYH 96
Query: 94 IQYACKRIAAIVASPFSCSIGKQTGVGAGGGGGSSGRDAPKPLDIVSNEIILSSLKKSGK 153
+Q+ACKRIA++VASPF+ S+GK + + G S RDAPKPLDIVSN+I+LSSL+ SGK
Sbjct: 97 LQHACKRIASLVASPFNSSLGKLSVNSSSG----SDRDAPKPLDIVSNDIVLSSLRNSGK 152
Query: 154 VAVMASEENDAPTWIIDDGPYVVVTDPLDGSRNIDASIPTGTIFGIYNRLEELDNLPTEE 213
VAVMASEEND+PTWI DDGPYVVV DPLDGSRNIDASIPTGTIFGIYNRL ELD+LP EE
Sbjct: 153 VAVMASEENDSPTWIKDDGPYVVVVDPLDGSRNIDASIPTGTIFGIYNRLVELDHLPVEE 212
Query: 214 KAQLNSLQSGSKLIAAGYVLYSSATILCVSFGSGTQAFTLDHSTGDFILTNPSIKIPPRG 273
KA+LNSLQ GS+L+A+GYVLYSSATI CV+ GSGT AFTLDHSTG+F+LT+ +IKIP RG
Sbjct: 213 KAELNSLQRGSRLVASGYVLYSSATIFCVTLGSGTHAFTLDHSTGEFVLTHQNIKIPTRG 272
Query: 274 QIYSVNDARYFDWPEGLRQYIDTVRQGKGRYPKKYSARYICSLVADLHRTLMYGGVAMNP 333
QIYSVNDARYFDWPEGLR+YIDTVRQGKG+ PKKYSARYICSLVADLHRTL+YGGVAMNP
Sbjct: 273 QIYSVNDARYFDWPEGLRKYIDTVRQGKGQNPKKYSARYICSLVADLHRTLLYGGVAMNP 332
Query: 334 RDHLRLVYEANPLSFLVEQAGGRGSDGKNRILSLQPVKLHQRLPLFLGSLEDMEELESFG 393
RDHLRLVYE NPL+FLVEQAGG+ SDGK ILS+QPVKLHQRLPLFLGSLED+ ELES+G
Sbjct: 333 RDHLRLVYEGNPLAFLVEQAGGKSSDGKRGILSIQPVKLHQRLPLFLGSLEDVAELESYG 392
Query: 394 DVQQKVNPGYEV 405
DVQQ VNPGYEV
Sbjct: 393 DVQQTVNPGYEV 404
>At3g54050 fructose-bisphosphatase precursor
Length = 417
Score = 266 bits (680), Expect = 2e-71
Identities = 146/328 (44%), Positives = 211/328 (63%), Gaps = 24/328 (7%)
Query: 82 DLKDDLVVLLDHIQYACKRIAAIVASPFSCSIGKQTGVGAGGGGGSSGRDAPKPLDIVSN 141
++ +L +++ I ACK+IA++V I TGV G G D K LD++SN
Sbjct: 95 EIDAELTIVMSSISLACKQIASLVQR---AGISNLTGVQ--GAVNIQGEDQKK-LDVISN 148
Query: 142 EIILSSLKKSGKVAVMASEENDAPTWIIDD--GPYVVVTDPLDGSRNIDASIPTGTIFGI 199
E+ + L+ SG+ ++ASEE D P + + G YVVV DPLDGS NIDA++ TG+IFGI
Sbjct: 149 EVFSNCLRSSGRTGIIASEEEDVPVAVEESYSGNYVVVFDPLDGSSNIDAAVSTGSIFGI 208
Query: 200 YNRLEEL-----DNLPT----EEKAQLNSLQSGSKLIAAGYVLYSSATILCVSFGSGTQA 250
Y+ +E D++ E++ +N Q G+ L+AAGY +YSS+ I ++ G G +
Sbjct: 209 YSPNDECIVDDSDDISALGSEEQRCIVNVCQPGNNLLAAGYCMYSSSVIFVLTLGKGVFS 268
Query: 251 FTLDHSTGDFILTNPSIKIPPRGQIYSVNDARYFDWPEGLRQYIDTVRQGKGRYPKKYSA 310
FTLD G+F+LT +I+IP G+IYS N+ Y W + L++YID ++ G K YSA
Sbjct: 269 FTLDPMYGEFVLTQENIEIPKAGRIYSFNEGNYQMWDDKLKKYIDDLKD-PGPTGKPYSA 327
Query: 311 RYICSLVADLHRTLMYGGVAMNPRD------HLRLVYEANPLSFLVEQAGGRGSDGKNRI 364
RYI SLV D HRTL+YGG+ PRD LRL+YE P+SF+VEQAGG+GSDG +R+
Sbjct: 328 RYIGSLVGDFHRTLLYGGIYGYPRDAKSKNGKLRLLYECAPMSFIVEQAGGKGSDGHSRV 387
Query: 365 LSLQPVKLHQRLPLFLGSLEDMEELESF 392
L +QP ++HQR+PL++GS E++E+LE +
Sbjct: 388 LDIQPTEIHQRVPLYIGSTEEVEKLEKY 415
>At1g43670 fructose 1,6-bisphosphatase, putative
Length = 341
Score = 243 bits (620), Expect = 1e-64
Identities = 137/329 (41%), Positives = 200/329 (60%), Gaps = 29/329 (8%)
Query: 84 KDDLVVLLDHIQYACKRIAAIVASPFSCSIGKQTG----VGAGGGGGSSGRDAPKPLDIV 139
+ D +LL HI CK F CS + G +G G G + K LD++
Sbjct: 30 RGDFTILLSHIVLGCK---------FVCSAVNKAGLAKLIGLAGETNIQGEEQKK-LDVL 79
Query: 140 SNEIILSSLKKSGKVAVMASEENDAPTWIIDD--GPYVVVTDPLDGSRNIDASIPTGTIF 197
SN++ +++L SG+ +V+ SEE++ T++ G Y VV DPLDGS NID + GTIF
Sbjct: 80 SNDVFVNALVSSGRTSVLVSEEDEEATFVEPSKRGKYCVVFDPLDGSSNIDCGVSIGTIF 139
Query: 198 GIYNRLEELDNLPTEEKAQLNSLQSGSKLIAAGYVLYSSATILCVSFGSGTQAFTLDHST 257
GIY LD+ T+E + L+ G++++AAGY +Y S+ +L +S G+G FTLD S
Sbjct: 140 GIYT----LDH--TDEPTTADVLKPGNEMVAAGYCMYGSSCMLVLSTGTGVHGFTLDPSL 193
Query: 258 GDFILTNPSIKIPPRGQIYSVNDARYFDWPEGLRQYIDTVRQGKGRYPKKYSARYICSLV 317
G+FILT+P IKIP +G IYSVN+ +W +Y++ + K P K S RY+ S+V
Sbjct: 194 GEFILTHPDIKIPNKGNIYSVNEGNAQNWDGPTTKYVEKCKFPKDGSPAK-SLRYVGSMV 252
Query: 318 ADLHRTLMYGGVAMNPRD------HLRLVYEANPLSFLVEQAGGRGSDGKNRILSLQPVK 371
AD+HRTL+YGG+ + P D LR++YE P+SFL+EQAGG+ GK R L L P K
Sbjct: 253 ADVHRTLLYGGIFLYPADKKSPNGKLRVLYEVFPMSFLMEQAGGQAFTGKKRALDLVPEK 312
Query: 372 LHQRLPLFLGSLEDMEELESFGDVQQKVN 400
+H+R P+FLGS +D+EE+++ ++K N
Sbjct: 313 IHERSPIFLGSYDDVEEIKALYAEEEKKN 341
>At3g55800 sedoheptulose-bisphosphatase precursor
Length = 393
Score = 68.6 bits (166), Expect = 6e-12
Identities = 74/276 (26%), Positives = 117/276 (41%), Gaps = 43/276 (15%)
Query: 131 DAPKPLDIVSNEIILSSLKKSGKVAVMASEENDAPTWIIDDGP----YVVVTDPLDGSRN 186
D +D+++++++ +L+ S SEE P GP + V DPLDGS
Sbjct: 127 DEQLAVDMLADKLLFEALQYSHVCKYACSEE--VPELQDMGGPVEGGFSVAFDPLDGSSI 184
Query: 187 IDASIPTGTIFGIYNRLEELDNLPTEEKAQLNSLQSGSKLIAAGYVLYSSAT--ILCVSF 244
+D + GTIFG++ P ++ + +G +AA +Y T +L V
Sbjct: 185 VDTNFTVGTIFGVW---------PGDKLTGI----TGGDQVAAAMGIYGPRTTYVLAVKG 231
Query: 245 GSGTQAFTL-DHSTGDFILTNPSIKIPPRGQIYSVNDARYFDWPEGLRQYIDTVRQGK-- 301
GT F L D + I G+++S P LR D K
Sbjct: 232 FPGTHEFLLLDEGKWQHVKETTEIA---EGKMFS---------PGNLRATFDNSEYSKLI 279
Query: 302 GRYPK-KYSARYICSLVADLHRTLMYG-GVAMN-----PRDHLRLVYEANPLSFLVEQAG 354
Y K KY+ RY +V D+++ ++ G+ N + LRL++E PL L+E AG
Sbjct: 280 DYYVKEKYTLRYTGGMVPDVNQIIVKEKGIFTNVTSPTAKAKLRLLFEVAPLGLLIENAG 339
Query: 355 GRGSDGKNRILSLQPVKLHQRLPLFLGSLEDMEELE 390
G SDG +L + L R + GS ++ E
Sbjct: 340 GFSSDGHKSVLDKTIINLDDRTQVAYGSKNEIIRFE 375
>At5g35160 unknown protein
Length = 627
Score = 32.7 bits (73), Expect = 0.37
Identities = 21/49 (42%), Positives = 28/49 (56%), Gaps = 4/49 (8%)
Query: 202 RLEELDNLPTEEKAQLNSLQSGSKLIAAG-YVLYSSATILCVSFGSGTQ 249
R EELD E +AQ+N SG KL+ + S+A++LCV G G Q
Sbjct: 291 RYEELDK---EAQAQMNEELSGWKLVVGDVFRAPSNASLLCVMVGDGVQ 336
>At5g35100 putative protein
Length = 281
Score = 32.3 bits (72), Expect = 0.48
Identities = 25/108 (23%), Positives = 51/108 (47%), Gaps = 11/108 (10%)
Query: 120 GAGGGG-------GSSGRDAPKPLDIVSNEIILSSLKKSGKVAVMASEENDAPTWIID-- 170
G GGG G S RD P+ D+V+++ L ++G V++ SE +D +D
Sbjct: 123 GRQGGGRRDTAEVGYSLRDLPRNTDVVNSKAFLLPHARAGVVSLCLSENDDDDDIRLDPD 182
Query: 171 --DGPYVVVTDPLDGSRNIDASIPTGTIFGIYNRLEELDNLPTEEKAQ 216
+ +++ T P + +I GT+ + + + ++PT + ++
Sbjct: 183 YRNVEFLITTGPGPSPQLDGGNIVFGTVLEGLDVVTSISSIPTYKPSE 230
>At2g37240 unknown protein
Length = 248
Score = 31.6 bits (70), Expect = 0.82
Identities = 23/64 (35%), Positives = 30/64 (45%), Gaps = 4/64 (6%)
Query: 93 HIQYACKRIAAIVASPFSCSIGKQTGVGAGGGGGSSGRDAPKPLDIVSNEIILSSLKKSG 152
H + R +A+V S + G +G G G G S D K LD+ NEI +S L K
Sbjct: 38 HFRSVSLRRSAVVVSAIT---GASSGAGIGKGTADS-LDTVKVLDLRGNEIPISDLWKDR 93
Query: 153 KVAV 156
K V
Sbjct: 94 KAVV 97
>At1g67035 putative protein
Length = 229
Score = 30.0 bits (66), Expect = 2.4
Identities = 29/111 (26%), Positives = 46/111 (41%), Gaps = 30/111 (27%)
Query: 25 KTPLTTPYCTPRLASISGSAKMRPLRALGGSSSSAGGDGDDGDGVVTLIEYLGKEGIDLK 84
+T LT P R A++ + +R GS S++ G+ K+GI +
Sbjct: 135 RTELTPPSMKSRNAAVFAGSVIR------GSVSASAGE---------------KKGIGMM 173
Query: 85 DDLVVLLDHIQYACKRIAAIVASPFSCSIGKQTGVGAGG-----GGGSSGR 130
+D + K+I+ AS + GK+ +G GG GGG GR
Sbjct: 174 RKSYAAVDDL----KKISMAAASAINGGGGKRRSIGGGGGRKSLGGGGGGR 220
>At5g60940 cleavage stimulation factor 50K chain
Length = 429
Score = 29.6 bits (65), Expect = 3.1
Identities = 13/33 (39%), Positives = 18/33 (54%)
Query: 136 LDIVSNEIILSSLKKSGKVAVMASEENDAPTWI 168
+D SNE++ + + KVA S N AP WI
Sbjct: 372 IDEASNEVVTWDARTADKVAKWPSNHNGAPRWI 404
>At3g19020 hypothetical protein
Length = 951
Score = 29.6 bits (65), Expect = 3.1
Identities = 16/41 (39%), Positives = 20/41 (48%), Gaps = 3/41 (7%)
Query: 95 QYACKRIAAIVASPFSCSIGKQTGVGAGGGGGSSGRDAPKP 135
Q + K +V+ P CS K G GGGGGS+ P P
Sbjct: 371 QKSAKECLPVVSRPVDCSKDKCAG---GGGGGSNPSPKPTP 408
>At1g01840 putative protein
Length = 152
Score = 29.6 bits (65), Expect = 3.1
Identities = 14/38 (36%), Positives = 24/38 (62%), Gaps = 1/38 (2%)
Query: 360 GKNRILSLQPVKL-HQRLPLFLGSLEDMEELESFGDVQ 396
G+N++L + +++ HQRL FL S+ E +SF V+
Sbjct: 29 GQNQLLQISSLRIAHQRLSAFLASIPTAEAEKSFSSVE 66
>At2g32710 cyclin-dependent kinase inhibitor (ACK2)
Length = 289
Score = 29.3 bits (64), Expect = 4.0
Identities = 12/27 (44%), Positives = 15/27 (55%)
Query: 114 GKQTGVGAGGGGGSSGRDAPKPLDIVS 140
G G G GGGGG G + +D+VS
Sbjct: 12 GAGAGAGGGGGGGGGGESSIALMDVVS 38
>At2g26120 hypothetical protein
Length = 104
Score = 29.3 bits (64), Expect = 4.0
Identities = 14/32 (43%), Positives = 19/32 (58%)
Query: 96 YACKRIAAIVASPFSCSIGKQTGVGAGGGGGS 127
++ K++ A+V S S G G G GGGGGS
Sbjct: 69 FSGKKVPAVVFRRVSSSDGGGRGSGGGGGGGS 100
>At2g11005 putative protein
Length = 170
Score = 29.3 bits (64), Expect = 4.0
Identities = 26/89 (29%), Positives = 35/89 (39%), Gaps = 5/89 (5%)
Query: 53 GGSSSSAGGDGDDG---DGVVTLI-EYLGKEGIDLKDDLVVLLDHIQYACKRIAAIVASP 108
GG S GG GD+G DGVV L+ ++ G+ VVL+ A + IV
Sbjct: 77 GGGSRDGGGSGDNGNTDDGVVALVMSFVLVTGV-APTATVVLMTVAVAAVVGVVMIVVVV 135
Query: 109 FSCSIGKQTGVGAGGGGGSSGRDAPKPLD 137
+ G G GS G +D
Sbjct: 136 VNAGDVDDGNSGGGNDDGSGGSGTKNDID 164
>At4g29240 extensin-like protein
Length = 415
Score = 28.9 bits (63), Expect = 5.3
Identities = 12/29 (41%), Positives = 16/29 (54%)
Query: 101 IAAIVASPFSCSIGKQTGVGAGGGGGSSG 129
+ +V + S +G GVG GGGGG G
Sbjct: 20 LGGLVQTDASFGVGGGVGVGIGGGGGGGG 48
>At4g11660 heat shock transcription factor - like protein
Length = 377
Score = 28.9 bits (63), Expect = 5.3
Identities = 14/32 (43%), Positives = 17/32 (52%), Gaps = 3/32 (9%)
Query: 101 IAAIVASPFSCSIGKQ---TGVGAGGGGGSSG 129
+A + CS G +G GAGGGGG SG
Sbjct: 12 VAGVGGGGAGCSAGNSGGSSGCGAGGGGGGSG 43
>At3g06130 unknown protein
Length = 473
Score = 28.9 bits (63), Expect = 5.3
Identities = 11/21 (52%), Positives = 14/21 (66%)
Query: 114 GKQTGVGAGGGGGSSGRDAPK 134
G++ G G GGGGG +APK
Sbjct: 116 GQKNGGGGGGGGGGGNSNAPK 136
>At2g03300 putative disease resistance protein
Length = 203
Score = 28.9 bits (63), Expect = 5.3
Identities = 14/37 (37%), Positives = 21/37 (55%)
Query: 336 HLRLVYEANPLSFLVEQAGGRGSDGKNRILSLQPVKL 372
HL +E N ++F V++ RG D KN L +Q K+
Sbjct: 25 HLIDAFERNEINFFVDKYEQRGKDLKNLFLRIQESKI 61
>At2g03030 putative disease resistance protein
Length = 204
Score = 28.9 bits (63), Expect = 5.3
Identities = 14/37 (37%), Positives = 21/37 (55%)
Query: 336 HLRLVYEANPLSFLVEQAGGRGSDGKNRILSLQPVKL 372
HL +E N ++F V++ RG D KN L +Q K+
Sbjct: 25 HLIDAFERNEINFFVDKYEQRGKDLKNLFLRIQESKI 61
>At1g52070 unknown protein
Length = 315
Score = 28.9 bits (63), Expect = 5.3
Identities = 19/49 (38%), Positives = 24/49 (48%), Gaps = 1/49 (2%)
Query: 41 SGSAKMRPLRALGGSSSSAGGDGDDGDGVVTLIEYLGKEGID-LKDDLV 88
+G AK + L A+GG DG D D V + G EGI +K D V
Sbjct: 18 TGLAKAQKLDAIGGKGGKQWDDGADHDNVAKVYIRGGLEGIQYIKFDYV 66
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.316 0.137 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,967,104
Number of Sequences: 26719
Number of extensions: 473386
Number of successful extensions: 2781
Number of sequences better than 10.0: 27
Number of HSP's better than 10.0 without gapping: 17
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 2646
Number of HSP's gapped (non-prelim): 100
length of query: 405
length of database: 11,318,596
effective HSP length: 102
effective length of query: 303
effective length of database: 8,593,258
effective search space: 2603757174
effective search space used: 2603757174
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0100a.12