BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0098b.4
(670 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g10740 putative alpha-L-arabinofuranosidase 961 0.0
At5g26120 arabinosidase - like protein 899 0.0
At5g05010 coatomer delta subunit (delta-coat protein) (delta-COP) 34 0.30
At4g25790 putative pathogenesis-related protein 33 0.67
At3g25545 unknown protein 32 1.1
At5g13950 unknown protein 31 2.0
At4g39430 hypothetical protein 30 4.4
At5g53570 GTPase activator protein of Rab-like small GTPases-lik... 30 5.7
At5g11000 unknown protein 30 5.7
At5g33280 chloride channel-like protein 29 7.4
At1g03060 putataive transport protein 29 7.4
At5g13440 ubiquinol--cytochrome-c reductase - like protein 29 9.7
At4g14280 hypothetical protein 29 9.7
At3g43190 sucrose synthase like protein 29 9.7
At3g25760 ERD12 protein (ERD12) 29 9.7
At3g05210 putative nucleotide repair protein 29 9.7
>At3g10740 putative alpha-L-arabinofuranosidase
Length = 678
Score = 961 bits (2484), Expect = 0.0
Identities = 462/636 (72%), Positives = 540/636 (84%), Gaps = 8/636 (1%)
Query: 33 TLTVDAHETSGKPISETLFGLFFEEINHAGAGGLWAELVSNRGFEAGGPNIPSNIDPWSI 92
TL VDA G+PI ETLFG+FFEEINHAGAGGLWAELVSNRGFEAGG N PSNI PWSI
Sbjct: 42 TLQVDASNGGGRPIPETLFGIFFEEINHAGAGGLWAELVSNRGFEAGGQNTPSNIWPWSI 101
Query: 93 IGNESFINVETDRTSCFDRNKIALRLEVLCDSKGDNICPADGVGVFNPGFWGMNIEQGKK 152
+G+ S I V TDR+SCF+RNKIALR++VLCDSKG CP+ GVGV+NPG+WGMNIE+GKK
Sbjct: 102 VGDHSSIYVATDRSSCFERNKIALRMDVLCDSKG---CPSGGVGVYNPGYWGMNIEEGKK 158
Query: 153 YKVVFYVRSTGSLDLTVSLTGSNG--VLASNVITGSASDFSNWKKVETLLEAKATNHNSR 210
YKV YVRSTG +DL+VSLT SNG LAS I SASD S W K E LLEAKAT+ ++R
Sbjct: 159 YKVALYVRSTGDIDLSVSLTSSNGSRTLASEKIIASASDVSKWIKKEVLLEAKATDPSAR 218
Query: 211 LQLTTTAKGVIWLDQVSAMPLDTYKGRGFRSELVGMLADLKPRFIRFPGGCFVEGEWLRN 270
LQLTTT KG IW+DQVSAMP+DT+KG GFR++L M+AD+KPRFIRFPGGCFVEGEWL N
Sbjct: 219 LQLTTTKKGSIWIDQVSAMPVDTHKGHGFRNDLFQMMADIKPRFIRFPGGCFVEGEWLSN 278
Query: 271 AFRWKESIGPWEERPGHFGDVWKYWTDDGLGYFEFLQLAEDLGALPIWVFNNGVSHNDEV 330
AFRWKE++GPWEERPGHFGDVWKYWTDDGLG+FEF Q+AED+GA PIWVFNNG+SHNDEV
Sbjct: 279 AFRWKETVGPWEERPGHFGDVWKYWTDDGLGHFEFFQMAEDIGAAPIWVFNNGISHNDEV 338
Query: 331 DTSAVLPFVQEALDGIEFARGDPTSKWGSIRASMGHPEPFNLKYVAVGNEDCGKKNYRGN 390
+T++++PFVQEALDGIEFARGD S WGS+RA MG EPF LKYVA+GNEDCGK YRGN
Sbjct: 339 ETASIMPFVQEALDGIEFARGDANSTWGSVRAKMGRQEPFELKYVAIGNEDCGKTYYRGN 398
Query: 391 YLKFYSAIRSAYPDIQIISNCDGSSRPLDHPADIYDYHIYTNANDMFSRSSTFNTALRSG 450
Y+ FY AI+ AYPDI+IISNCDGSS PLDHPAD YDYHIYT+A+++FS F+ R G
Sbjct: 399 YIVFYDAIKKAYPDIKIISNCDGSSHPLDHPADYYDYHIYTSASNLFSMYHQFDRTSRKG 458
Query: 451 PKAFVSEYAVTGNDAGTGSLLAAIAEAGFLLGLEKNSDIVKMVSYAPLFVNANDRRWNPD 510
PKAFVSEYAVTG DAGTGSLLA++AEA FL+GLEKNSDIV+M SYAPLFVN NDRRWNPD
Sbjct: 459 PKAFVSEYAVTGKDAGTGSLLASLAEAAFLIGLEKNSDIVEMASYAPLFVNTNDRRWNPD 518
Query: 511 AIVFNSFQSYGTPSYWVQLFFSESSGATLLNSSLQTSSSSSLIASAINWQSSADKKNYIR 570
AIVFNS YGTPSYWVQ FF+ESSGATLL S+L+ +S+SL+ASAI+W+++ K+YIR
Sbjct: 519 AIVFNSSHLYGTPSYWVQRFFAESSGATLLTSTLK-GNSTSLVASAISWKNNG--KDYIR 575
Query: 571 IKVVNFGANTVNLKISLNGLDPNSLQPSGSTRTVLTSANVMDENSFSQPKKVVPIQSLLQ 630
IK VNFGAN+ N+++ + GLDPN ++ SGS +TVLTS NVMDENSFSQP+KVVP +SLL+
Sbjct: 576 IKAVNFGANSENMQVLVTGLDPNVMRVSGSKKTVLTSTNVMDENSFSQPEKVVPHESLLE 635
Query: 631 NVGKEMNVVVPPHSFSSFDLLKESSNLKMVGGGSST 666
++M VV+PPHSFSSFDLLKES+ ++M SS+
Sbjct: 636 LAEEDMTVVLPPHSFSSFDLLKESAKIRMPISDSSS 671
>At5g26120 arabinosidase - like protein
Length = 674
Score = 899 bits (2324), Expect = 0.0
Identities = 448/654 (68%), Positives = 524/654 (79%), Gaps = 14/654 (2%)
Query: 7 SFIVAALQLCIAVSLCAIKVNADLNATLTVDAHETSGKPISETLFGLFFEEINHAGAGGL 66
SFI+ + + +LC + D TL VDA + +PI ETLFG+FFEEINHAGAGGL
Sbjct: 17 SFILGSFS--VYQTLCLVDAQEDAIVTLQVDASNVTRRPIPETLFGIFFEEINHAGAGGL 74
Query: 67 WAELVSNRGFEAGGPNIPSNIDPWSIIGNESFINVETDRTSCFDRNKIALRLEVLCDSKG 126
WAELVSNRGFEAGG IPSNI PWSIIG+ES I V TDR+SCF+RNKIALR+EVLCDS
Sbjct: 75 WAELVSNRGFEAGGQIIPSNIWPWSIIGDESSIYVVTDRSSCFERNKIALRMEVLCDS-- 132
Query: 127 DNICPADGVGVFNPGFWGMNIEQGKKYKVVFYVRSTGSLDLTVSLTGSNG--VLASNVIT 184
N CP GVGV+NPG+WGMNIE+GKKYKVV YVRSTG +D++VS T SNG LAS I
Sbjct: 133 -NSCPLGGVGVYNPGYWGMNIEEGKKYKVVLYVRSTGDIDVSVSFTSSNGSVTLASENII 191
Query: 185 GSASDFSNWKKVETLLEAKATNHNSRLQLTTTAKGVIWLDQVSAMPLDTYKGRGFRSELV 244
ASD NW K E LLEA T++ +RLQ TTT KG IW DQVSAMP+DTYKG GFR++L
Sbjct: 192 ALASDLLNWTKKEMLLEANGTDNGARLQFTTTKKGSIWFDQVSAMPMDTYKGHGFRNDLF 251
Query: 245 GMLADLKPRFIRFPGGCFVEGEWLRNAFRWKESIGPWEERPGHFGDVWKYWTDDGLGYFE 304
M+ DLKPRFIRFPGGCFVEG+WL NAFRWKE++ WEERPGH+GDVWKYWTDDGLG+FE
Sbjct: 252 QMMVDLKPRFIRFPGGCFVEGDWLGNAFRWKETVRAWEERPGHYGDVWKYWTDDGLGHFE 311
Query: 305 FLQLAEDLGALPIWVFNNGVSHNDEVDTSAVLPFVQEALDGIEFARGDPTSKWGSIRASM 364
F QLAEDLGA PIWVFNNG+SHND+V+T V+PFVQEA+DGIEFARGD S WGS+RA+M
Sbjct: 312 FFQLAEDLGASPIWVFNNGISHNDQVETKNVMPFVQEAIDGIEFARGDSNSTWGSVRAAM 371
Query: 365 GHPEPFNLKYVAVGNEDCGKKNYRGNYLKFYSAIRSAYPDIQIISNCDGSSRPLDHPADI 424
GHPEPF LKYVAVGNEDC K YRGNYL+FY+AI+ AYPDI+IISNCD S++PLDHPAD
Sbjct: 372 GHPEPFELKYVAVGNEDCFKSYYRGNYLEFYNAIKKAYPDIKIISNCDASAKPLDHPADY 431
Query: 425 YDYHIYTNANDMFSRSSTFNTALRSGPKAFVSEYAVTGNDAGTGSLLAAIAEAGFLLGLE 484
+DYHIYT A D+FS+S F+ R+GPKAFVSEYAV DA G+LLAA+ EA FLLGLE
Sbjct: 432 FDYHIYTLARDLFSKSHDFDNTPRNGPKAFVSEYAVNKADAKNGNLLAALGEAAFLLGLE 491
Query: 485 KNSDIVKMVSYAPLFVNANDRRWNPDAIVFNSFQSYGTPSYWVQLFFSESSGATLLNSSL 544
KNSDIV+MVSYAPLFVN NDRRW PDAIVFNS YGTPSYWVQ FF+ESSGATLLNS+L
Sbjct: 492 KNSDIVEMVSYAPLFVNTNDRRWIPDAIVFNSSHLYGTPSYWVQHFFTESSGATLLNSTL 551
Query: 545 QTSSSSSLIASAINWQSSADKKNYIRIKVVNFGANTVNLKISLNGLDPNSLQPSGSTRTV 604
+ +SS+ ASAI++Q++ K+YI+IK VNFG +VNLK+++ GL + GS + V
Sbjct: 552 K-GKTSSVEASAISFQTNG--KDYIQIKAVNFGEQSVNLKVAVTGL---MAKFYGSKKKV 605
Query: 605 LTSANVMDENSFSQPKKVVPIQSLLQNVGKE-MNVVVPPHSFSSFDLLKESSNL 657
LTSA+VMDENSFS P +VP +SLL+ +E + V+PPHSFSSFDLL ES N+
Sbjct: 606 LTSASVMDENSFSNPNMIVPQESLLEMTEQEDLMFVLPPHSFSSFDLLTESENV 659
>At5g05010 coatomer delta subunit (delta-coat protein) (delta-COP)
Length = 527
Score = 33.9 bits (76), Expect = 0.30
Identities = 20/80 (25%), Positives = 40/80 (50%), Gaps = 1/80 (1%)
Query: 62 GAGGLWAELVSNRGFEAGGPNIPSNIDPW-SIIGNESFINVETDRTSCFDRNKIALRLEV 120
G GG L+ R A +P I+ W S+ GNE+++++E + +S FD + + + +
Sbjct: 367 GQGGDGVGLLRWRMQRADESMVPLTINCWPSVSGNETYVSLEYEASSMFDLTNVIISVPL 426
Query: 121 LCDSKGDNICPADGVGVFNP 140
+ ++ DG ++P
Sbjct: 427 PALREAPSVRQCDGEWRYDP 446
>At4g25790 putative pathogenesis-related protein
Length = 210
Score = 32.7 bits (73), Expect = 0.67
Identities = 21/51 (41%), Positives = 29/51 (56%), Gaps = 6/51 (11%)
Query: 170 SLTGSNGVLASNVITGSASDFSNWKKVET-LLEAKATNHNSRLQLTTTAKG 219
SLT S G N+ GS SDF++ VE+ +EAK+ NH +T T +G
Sbjct: 116 SLTHSTGPYGENLFWGSGSDFTSTFAVESWTVEAKSYNH-----MTNTCEG 161
>At3g25545 unknown protein
Length = 267
Score = 32.0 bits (71), Expect = 1.1
Identities = 22/65 (33%), Positives = 30/65 (45%), Gaps = 1/65 (1%)
Query: 195 KVETLLEAKATNHNSRLQLTTTAKGVIWLDQVSAMPLDTYKGRGFRSELVGMLADLKPRF 254
K+ET E KAT H + L G + +D +SA L + + R LV + P
Sbjct: 164 KLETSAEKKATTHTGLVNLDYFPSGSV-IDGLSASLLSRCQLKNIRGTLVISWPEFGPSV 222
Query: 255 IRFPG 259
IRF G
Sbjct: 223 IRFAG 227
>At5g13950 unknown protein
Length = 939
Score = 31.2 bits (69), Expect = 2.0
Identities = 20/64 (31%), Positives = 30/64 (46%), Gaps = 10/64 (15%)
Query: 339 VQEALDGIEFARGDPTSKWG-SIRASMGHPEPFNLKYVAVGNEDC---GKKNYRGNYLKF 394
VQ LDG F G+P+ WG ++ + HP+ V E+C K+ Y N K+
Sbjct: 134 VQALLDGENFHFGNPSLDWGTAVCSGKAHPDQI------VSREECLRADKRRYYSNLEKY 187
Query: 395 YSAI 398
+ I
Sbjct: 188 HQDI 191
>At4g39430 hypothetical protein
Length = 405
Score = 30.0 bits (66), Expect = 4.4
Identities = 35/145 (24%), Positives = 59/145 (40%), Gaps = 15/145 (10%)
Query: 470 LLAAIAEAGFLLGLEKNSD-----IVKMVSYAPLFVNANDRRWN-----PDAIVFNSFQS 519
+L + A +LL + +D + +++ A +F RR+ D +F+S
Sbjct: 12 MLRVLFSAVYLLSRKDRNDNEISAVSRLLGLATMFATEMIRRYGLLEYRKDVYMFDS--- 68
Query: 520 YGTPSYWVQLFFSESSGATLLNSSLQTSSSSSLIASAINWQSSADKKNYIRIKVVNFGAN 579
P + + S ++ +S + S L+ N QS +K KVV +
Sbjct: 69 --KPRTQILSLPAVSLNIDVMENSRRLSEMGYLLEITRNIQSRITRKFKKLGKVVYYINT 126
Query: 580 TVNLKISLNGLDPNSLQPSGSTRTV 604
N + SLN +DPNSLQ V
Sbjct: 127 NGNNEKSLNLVDPNSLQDDSQLEIV 151
>At5g53570 GTPase activator protein of Rab-like small GTPases-like
protein
Length = 550
Score = 29.6 bits (65), Expect = 5.7
Identities = 23/76 (30%), Positives = 41/76 (53%), Gaps = 3/76 (3%)
Query: 530 FFSESSGATLLNSSLQTSSSSSLIASAINWQSSADKKNYIRIKVVNFGANTVNLKISL-- 587
+ S SSG + +SSL +SSSSSL +S+ + S++ +Y + ++ ++L+ L
Sbjct: 8 YTSTSSGNSSSSSSLPSSSSSSLPSSSSSSPPSSNSNSYSNSNSSSSSSSWIHLRSVLFV 67
Query: 588 -NGLDPNSLQPSGSTR 602
N P+S+ S R
Sbjct: 68 ANLSSPSSVTSSDRRR 83
>At5g11000 unknown protein
Length = 389
Score = 29.6 bits (65), Expect = 5.7
Identities = 19/63 (30%), Positives = 32/63 (50%), Gaps = 1/63 (1%)
Query: 507 WNPDAIVFNSFQSYGTPSYW-VQLFFSESSGATLLNSSLQTSSSSSLIASAINWQSSADK 565
W P NSF G + ++ F +S ++ +S +S+SS+ +S + W SSAD+
Sbjct: 302 WKPKKQCGNSFGIKGIVEWRKMRKRFVKSKRSSSSSSISMSSASSACSSSVMEWASSADE 361
Query: 566 KNY 568
Y
Sbjct: 362 AEY 364
>At5g33280 chloride channel-like protein
Length = 763
Score = 29.3 bits (64), Expect = 7.4
Identities = 14/33 (42%), Positives = 21/33 (63%), Gaps = 1/33 (3%)
Query: 177 VLASNVITGSASDFSNWKKVETLLEA-KATNHN 208
+L +V+TG F+ +KVET++ K TNHN
Sbjct: 569 LLVGDVVTGPLQVFNGIEKVETIVHVLKTTNHN 601
>At1g03060 putataive transport protein
Length = 3601
Score = 29.3 bits (64), Expect = 7.4
Identities = 28/108 (25%), Positives = 44/108 (39%), Gaps = 14/108 (12%)
Query: 503 NDRRWNPDAIVFNSFQSYGTPSYWVQLFFSESSGATLLNSSLQTSSSSSL---------- 552
+D P A FQ + VQ S+SS + + S S SSL
Sbjct: 2033 DDHHVGPSASSERDFQDFTGNPVQVQATDSQSSASFPMIESPLLSEKSSLKVSFTPSPSP 2092
Query: 553 IASAINWQSSADKKNYIRIKVVNFGANTVNLKISLNGLDPNSLQPSGS 600
+ + +W S NY K G+ ++ +S+N +D +S + SGS
Sbjct: 2093 VVALASWLGS----NYNESKSSTLGSPSLESYVSVNEVDASSERKSGS 2136
>At5g13440 ubiquinol--cytochrome-c reductase - like protein
Length = 274
Score = 28.9 bits (63), Expect = 9.7
Identities = 15/37 (40%), Positives = 21/37 (56%)
Query: 441 STFNTALRSGPKAFVSEYAVTGNDAGTGSLLAAIAEA 477
S+FN+ RS + F S+ GN+ G GS + A EA
Sbjct: 49 SSFNSYHRSVIRGFASQVITQGNEIGFGSEVPATVEA 85
>At4g14280 hypothetical protein
Length = 848
Score = 28.9 bits (63), Expect = 9.7
Identities = 21/66 (31%), Positives = 30/66 (44%), Gaps = 2/66 (3%)
Query: 146 NIEQGKKYKVVFYVRSTGSLDLTVSLTGSNGVLASNVITGSASDFSNWKKVETLLEAKAT 205
N+ + YK++ RS L VS TG+ G I+G SN + ETL K+
Sbjct: 477 NVAVAEPYKILAVKRSLKLLKKLVSTTGTTGKNLRMTISGIVFTVSNIR--ETLHHGKSQ 534
Query: 206 NHNSRL 211
H +L
Sbjct: 535 PHLQKL 540
>At3g43190 sucrose synthase like protein
Length = 808
Score = 28.9 bits (63), Expect = 9.7
Identities = 26/97 (26%), Positives = 42/97 (42%), Gaps = 17/97 (17%)
Query: 299 GLGYFEFLQLAEDLGALP------------IWVFNNGVSHN---DEVDTSAVLPFVQEAL 343
G +FEFL+ A++ LP +W + H+ +E+ S L F +E +
Sbjct: 66 GGAFFEFLRSAQEAIVLPPFVALAVRPRPGVWEYVRVNLHDLVVEELQASEYLQFKEELV 125
Query: 344 DGIEFARGDPTSKWGSIRASMGHPEPFNLKYVAVGNE 380
DGI+ G+ T + + P P KY+ G E
Sbjct: 126 DGIK--NGNFTLELDFEPFNAAFPRPTLNKYIGDGVE 160
>At3g25760 ERD12 protein (ERD12)
Length = 254
Score = 28.9 bits (63), Expect = 9.7
Identities = 26/92 (28%), Positives = 40/92 (43%), Gaps = 20/92 (21%)
Query: 546 TSSSSSLIASAINWQSSADKKNYIRIKVVNFGANTVNLKISLNGLDPNSLQPSGSTRTVL 605
T S S+ + +N S + K + R ++ F + N IS NG P S P+
Sbjct: 5 TISLQSISMTTLNNLSYS--KQFHRSSLLGFSKSFQNFGISSNG--PGSSSPT------- 53
Query: 606 TSANVMDENSFSQPKKVVPIQSLLQNVGKEMN 637
SF+ KK+ P ++L QN+G N
Sbjct: 54 ---------SFTPKKKLTPTRALSQNLGNTEN 76
>At3g05210 putative nucleotide repair protein
Length = 410
Score = 28.9 bits (63), Expect = 9.7
Identities = 18/68 (26%), Positives = 30/68 (43%)
Query: 593 NSLQPSGSTRTVLTSANVMDENSFSQPKKVVPIQSLLQNVGKEMNVVVPPHSFSSFDLLK 652
+S QPSG+++ +S+ + S V S+ N + N ++ H LLK
Sbjct: 80 SSSQPSGASQVPHSSSQTHQTDGASSSSTPVATGSVPSNTTQNRNAILVSHRQKGNPLLK 139
Query: 653 ESSNLKMV 660
N+K V
Sbjct: 140 HIRNVKWV 147
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.317 0.134 0.403
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,510,921
Number of Sequences: 26719
Number of extensions: 697923
Number of successful extensions: 1675
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 1659
Number of HSP's gapped (non-prelim): 19
length of query: 670
length of database: 11,318,596
effective HSP length: 106
effective length of query: 564
effective length of database: 8,486,382
effective search space: 4786319448
effective search space used: 4786319448
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 63 (28.9 bits)
Lotus: description of TM0098b.4