BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0098b.3
(287 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g10990 putative retrotransposon polyprotein 33 0.14
At4g11430 putative proline-rich protein 32 0.39
At2g31080 putative non-LTR retroelement reverse transcriptase 32 0.39
At4g25160 putative Ser/Thr protein kinase 31 0.88
At2g07730 putative non-LTR retroelement reverse transcriptase 31 0.88
At1g69810 unknown protein 31 0.88
At3g03770 unknown protein 30 1.1
At5g66730 zinc finger protein 30 1.5
At4g22360 unknown protein 30 1.5
At1g64790 hypothetical protein 30 1.5
At1g16610 arginine/serine-rich protein 30 1.5
At5g38690 unknown protein 30 2.0
At5g04810 membrane-associated salt-inducible protein-like 30 2.0
At4g36080 ATM-like protein 30 2.0
At2g31220 putative transcription factor BHLH10 29 2.6
At2g22350 putative non-LTR retroelement reverse transcriptase 29 2.6
At1g62760 hypothetical protein 29 2.6
At5g21280 putative protein 29 3.3
At5g15390 rRNA methylase - like protein 29 3.3
At1g07970 unknown protein 29 3.3
>At4g10990 putative retrotransposon polyprotein
Length = 1203
Score = 33.5 bits (75), Expect = 0.14
Identities = 30/113 (26%), Positives = 51/113 (44%), Gaps = 13/113 (11%)
Query: 95 LAMPHPLYDVLNKPTINDMASQVDKALLSSSSSSLSLSHPPPPPPSFTIATIVVVAIATG 154
L +P PL+ V + P +D+ + + A S+S+S+ S S PP P + + I T
Sbjct: 385 LPLPAPLHFVESMPLDDDLRADDNNA--STSNSASSASSIPPLPSTVNTQNTDALDIDTN 442
Query: 155 SITA------SNSTVVITNTHCHHHPPLMPLFIT-----EVTHTNIAPRKHVT 196
S+ + + ++ HC+ P L L T E ++I P+K T
Sbjct: 443 SVPIARPKRNAKAPAYLSEYHCNSVPFLSSLSPTTSTSIETPSSSIPPKKITT 495
>At4g11430 putative proline-rich protein
Length = 219
Score = 32.0 bits (71), Expect = 0.39
Identities = 26/67 (38%), Positives = 28/67 (40%), Gaps = 10/67 (14%)
Query: 132 SHPPPPPPSFTIATIV--VVAIATG------SITASNSTVVITNTHCHH--HPPLMPLFI 181
S PPPPPP TI V TG +T ITNT HH HPP I
Sbjct: 150 SPPPPPPPPPPPPTITPPVTTTTTGHHHHRPPPPPPATTTPITNTSDHHQLHPPPPATTI 209
Query: 182 TEVTHTN 188
T T +N
Sbjct: 210 TTTTISN 216
Score = 28.1 bits (61), Expect = 5.7
Identities = 22/73 (30%), Positives = 27/73 (36%), Gaps = 19/73 (26%)
Query: 134 PPPPPPSFTIATIVVVAIA------------------TGSITASNSTVVITNTHCHHHPP 175
PPPPPP TI V A +IT +T T H H PP
Sbjct: 124 PPPPPPPPTITPPVTTTTAGHHHHRRSPPPPPPPPPPPPTITPPVTTTT-TGHHHHRPPP 182
Query: 176 LMPLFITEVTHTN 188
P T +T+T+
Sbjct: 183 PPPATTTPITNTS 195
>At2g31080 putative non-LTR retroelement reverse transcriptase
Length = 1231
Score = 32.0 bits (71), Expect = 0.39
Identities = 21/74 (28%), Positives = 33/74 (44%), Gaps = 1/74 (1%)
Query: 29 LMSVDDIIRYSFSEWVASFSSFFGGSCSLYVELLAKKHGLLLDINLGYRWVICESDSTTV 88
L + IR EW+ F+ G + EL +GLL+ + G+R V + D V
Sbjct: 1085 LAAAGGAIRNGQGEWLGGFALNIGSCAAPLAELWGAYYGLLIAWDKGFRRVELDLDCKLV 1144
Query: 89 IHLLE-ALAMPHPL 101
+ L ++ HPL
Sbjct: 1145 VGFLSTGVSNAHPL 1158
>At4g25160 putative Ser/Thr protein kinase
Length = 835
Score = 30.8 bits (68), Expect = 0.88
Identities = 17/34 (50%), Positives = 21/34 (61%), Gaps = 4/34 (11%)
Query: 122 LSSSSSSLSLSHPPPPPPSFTIATIVVVAIATGS 155
+S S L+L PPPPPPS T VVVA++ S
Sbjct: 1 MSRSPDKLALPPPPPPPPSRT----VVVALSGSS 30
>At2g07730 putative non-LTR retroelement reverse transcriptase
Length = 970
Score = 30.8 bits (68), Expect = 0.88
Identities = 19/61 (31%), Positives = 29/61 (47%), Gaps = 1/61 (1%)
Query: 42 EWVASFSSFFGGSCSLYVELLAKKHGLLLDINLGYRWVICESDSTTVIHLLE-ALAMPHP 100
EW+ F+ G + EL +GLL+ + G+R V DS V+ L ++ HP
Sbjct: 837 EWLGGFALNIGSCDAPLAELWGAYYGLLIAWDKGFRRVELNLDSELVVGFLSTGISKAHP 896
Query: 101 L 101
L
Sbjct: 897 L 897
>At1g69810 unknown protein
Length = 387
Score = 30.8 bits (68), Expect = 0.88
Identities = 14/19 (73%), Positives = 15/19 (78%)
Query: 122 LSSSSSSLSLSHPPPPPPS 140
LSSSS SL+ S P PPPPS
Sbjct: 335 LSSSSFSLNFSSPDPPPPS 353
>At3g03770 unknown protein
Length = 802
Score = 30.4 bits (67), Expect = 1.1
Identities = 20/51 (39%), Positives = 27/51 (52%), Gaps = 11/51 (21%)
Query: 101 LYDVLNKPTINDM------ASQVDKALLSSSSSSLSLSHPPP-----PPPS 140
L D L +P+I D+ ASQV + L +S+ S +L P P PPPS
Sbjct: 732 LKDPLERPSIEDVLWNLQFASQVQEGWLQNSNPSSNLGSPSPAASSLPPPS 782
>At5g66730 zinc finger protein
Length = 500
Score = 30.0 bits (66), Expect = 1.5
Identities = 21/74 (28%), Positives = 35/74 (46%), Gaps = 2/74 (2%)
Query: 91 LLEALAMPHPLYDVLNKPTINDMASQVDKALLSSSSSSLSLSHPPPPPPSFTIATIVVVA 150
L E A H L T+ ++++ ++ SS SL PP PPS IA ++
Sbjct: 188 LAEESAKNHTQSKKLYPETVTRKNPEIEQKSPAAVESSPSL--PPSSPPSVAIAPAPAIS 245
Query: 151 IATGSITASNSTVV 164
+ T S+ +S+V+
Sbjct: 246 VETESVKIISSSVL 259
>At4g22360 unknown protein
Length = 385
Score = 30.0 bits (66), Expect = 1.5
Identities = 17/55 (30%), Positives = 28/55 (50%), Gaps = 4/55 (7%)
Query: 124 SSSSSLSLSHPPPPPPSF----TIATIVVVAIATGSITASNSTVVITNTHCHHHP 174
+S+SS+ SHPPPPP S + + V +A G T S+ + ++ +P
Sbjct: 67 ASASSVQQSHPPPPPSSHQQQNLHSGVNVPPMAKGHFTLSHPSQFSVSSQSQQYP 121
>At1g64790 hypothetical protein
Length = 2428
Score = 30.0 bits (66), Expect = 1.5
Identities = 15/69 (21%), Positives = 31/69 (44%), Gaps = 2/69 (2%)
Query: 111 NDMASQVDKALLSSSSSSLS--LSHPPPPPPSFTIATIVVVAIATGSITASNSTVVITNT 168
+D S ++SS +S H P PS + ++ I++ ++ S+ ++
Sbjct: 474 SDFLSITGDQIVSSRTSDADNPADHQAPFVPSVEVLVKALIVISSAAVAGPPSSWIVRAI 533
Query: 169 HCHHHPPLM 177
C HHP ++
Sbjct: 534 FCSHHPSIV 542
>At1g16610 arginine/serine-rich protein
Length = 414
Score = 30.0 bits (66), Expect = 1.5
Identities = 14/20 (70%), Positives = 15/20 (75%)
Query: 123 SSSSSSLSLSHPPPPPPSFT 142
SSS+SS S S PPPPPP T
Sbjct: 395 SSSNSSSSSSPPPPPPPRKT 414
>At5g38690 unknown protein
Length = 572
Score = 29.6 bits (65), Expect = 2.0
Identities = 21/93 (22%), Positives = 45/93 (47%), Gaps = 6/93 (6%)
Query: 193 KHVTSGADKERLVRGTEAGVPKVSLDDVVRWSVPFLMVERTMVQGL---TDREGKPEQEF 249
K + +G E +V ++ K+ L D V + ++++ + +G T +G E
Sbjct: 175 KDINNGCSNENVVV-KKSNPKKIKLSDSVHATTE--VIKKVLAEGKKKKTTAKGIKENNV 231
Query: 250 IGRNKFVKEKVQEKSEDRIEVDISYSTIVIRLN 282
+ K +K +++K ED++E+ I I I ++
Sbjct: 232 AEKIKRIKPALKKKEEDQVEIKIPQGNISITVS 264
>At5g04810 membrane-associated salt-inducible protein-like
Length = 952
Score = 29.6 bits (65), Expect = 2.0
Identities = 16/49 (32%), Positives = 26/49 (52%), Gaps = 8/49 (16%)
Query: 99 HPLYDVLNKPTINDMASQVDKALLSSSSSSLS--------LSHPPPPPP 139
+PL + N+ +++ + + +SS SSL+ LS PPPPPP
Sbjct: 81 NPLKGLTNRSSVSPLVQSEVSSKVSSFGSSLASKLRLSSKLSPPPPPPP 129
>At4g36080 ATM-like protein
Length = 3738
Score = 29.6 bits (65), Expect = 2.0
Identities = 31/113 (27%), Positives = 44/113 (38%), Gaps = 10/113 (8%)
Query: 40 FSEWVASFSSFFGGSCSLYVE--LLAKKHGLLLDINLGYR-WVICESDSTTVIHLLEALA 96
F V S F + L V ++ + D N+ Y WV+ +H E +A
Sbjct: 2428 FDSIVMKHSQFLSAASKLQVADVVIPLRELAHTDANVAYHLWVLVFPIVWATLHKEEQIA 2487
Query: 97 MPHPLYDVLNKPTINDMASQ---VDKALLSSSSSSLSLSHPPPPPPSFTIATI 146
+ P+ +L+K V +ALL L LSHP P PS I I
Sbjct: 2488 LAKPMISLLSKDYHKKQQGHRPNVVQALLEG----LQLSHPQPRMPSELIKYI 2536
>At2g31220 putative transcription factor BHLH10
Length = 458
Score = 29.3 bits (64), Expect = 2.6
Identities = 17/49 (34%), Positives = 22/49 (44%), Gaps = 9/49 (18%)
Query: 127 SSLSLSHPPPPPPSFTIATIVVVAIATGSITASNSTVVITNTHCHHHPP 175
SS S + PPPPPP +A GS + SN +V + H P
Sbjct: 27 SSFSQAEPPPPPPQVLVA---------GSTSNSNCSVEVEELSEFHLSP 66
>At2g22350 putative non-LTR retroelement reverse transcriptase
Length = 321
Score = 29.3 bits (64), Expect = 2.6
Identities = 24/81 (29%), Positives = 34/81 (41%), Gaps = 8/81 (9%)
Query: 35 IIRYSFSEWVASFSSFFGGSCSLYVELLAKKHGLLLDINLGYRWVICESDSTTVI-HLLE 93
++R W+ F+ G + EL +GL + G R V E DS V+ L
Sbjct: 181 VLRDHNGAWIGGFAVNIGVCSAPLAELWGVYYGLFIAWGRGARRVELEVDSKMVVGFLTT 240
Query: 94 ALAMPHPL-------YDVLNK 107
+A HPL YD L+K
Sbjct: 241 GIADSHPLSFLLRLCYDFLSK 261
>At1g62760 hypothetical protein
Length = 312
Score = 29.3 bits (64), Expect = 2.6
Identities = 18/55 (32%), Positives = 28/55 (50%)
Query: 108 PTINDMASQVDKALLSSSSSSLSLSHPPPPPPSFTIATIVVVAIATGSITASNST 162
P + +S +L SS LSLS PPPP + + + ++ ++ S T SN T
Sbjct: 89 PPSSSPSSAPPSSLSPSSPPPLSLSPSSPPPPPPSSSPLSSLSPSSSSSTYSNQT 143
Score = 29.3 bits (64), Expect = 2.6
Identities = 21/65 (32%), Positives = 33/65 (50%), Gaps = 3/65 (4%)
Query: 115 SQVDKALLSSSSS---SLSLSHPPPPPPSFTIATIVVVAIATGSITASNSTVVITNTHCH 171
S + LS SS SLS S PPPPPPS + + + +++ ++S S+ ++
Sbjct: 46 SSAPPSSLSPSSPPPLSLSPSSPPPPPPSSSPLSSLSPSLSPSPPSSSPSSAPPSSLSPS 105
Query: 172 HHPPL 176
PPL
Sbjct: 106 SPPPL 110
>At5g21280 putative protein
Length = 302
Score = 28.9 bits (63), Expect = 3.3
Identities = 24/65 (36%), Positives = 29/65 (43%), Gaps = 11/65 (16%)
Query: 92 LEALAMPHPLYDVLNKPTINDM------ASQVDKALLSSSSSSLSLSHP-----PPPPPS 140
L L PL LN ND S + SSSSSS+ ++P P PPP+
Sbjct: 131 LNFLLPNQPLGLNLNFQDFNDFIQTSSTTSSSSSSSTSSSSSSIFPTNPHIYSSPSPPPT 190
Query: 141 FTIAT 145
FT AT
Sbjct: 191 FTTAT 195
>At5g15390 rRNA methylase - like protein
Length = 350
Score = 28.9 bits (63), Expect = 3.3
Identities = 22/66 (33%), Positives = 33/66 (49%), Gaps = 3/66 (4%)
Query: 119 KALLSSSSSSLSLSHPPPPPPSFTIATIVVVAIATGSITASNSTVVITNTHCHHHP-PLM 177
K+L+ +S SS P P P+F+I I V A + S+ NS+ ++T H L
Sbjct: 5 KSLIRASISSFPFV--PLPNPNFSITFISVRAFSPLSVLHPNSSCIVTARRTFHGAVALS 62
Query: 178 PLFITE 183
P +TE
Sbjct: 63 PESLTE 68
>At1g07970 unknown protein
Length = 693
Score = 28.9 bits (63), Expect = 3.3
Identities = 15/60 (25%), Positives = 31/60 (51%), Gaps = 3/60 (5%)
Query: 103 DVLNKPTINDMASQVDKALLSSSSSSLSLSHPPPPPPSFTIATIVVVAIATGSITASNST 162
D+ + + + +++D+ + S+ + PPP SF +A+ V +TG+ A+ ST
Sbjct: 256 DITTEEQLEQLLAEIDEKITESAGK---MRTPPPTVGSFAMASPSTVGGSTGASGATRST 312
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.319 0.134 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,589,034
Number of Sequences: 26719
Number of extensions: 290790
Number of successful extensions: 2581
Number of sequences better than 10.0: 51
Number of HSP's better than 10.0 without gapping: 21
Number of HSP's successfully gapped in prelim test: 31
Number of HSP's that attempted gapping in prelim test: 2310
Number of HSP's gapped (non-prelim): 201
length of query: 287
length of database: 11,318,596
effective HSP length: 98
effective length of query: 189
effective length of database: 8,700,134
effective search space: 1644325326
effective search space used: 1644325326
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0098b.3