BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0091b.8
(200 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g15970 similar to cold acclimation protein WCOR413 [Triticum ... 258 1e-69
At3g50830 putative cold acclimation protein 248 2e-66
At4g37220 cold acclimation protein homolog 244 3e-65
At2g23680 similar to cold acclimation protein WCOR413 [Triticum ... 149 7e-37
At1g29395 unknown protein 60 8e-10
At1g29390 unknown protein 60 8e-10
At4g29140 unknown protein 32 0.29
At5g13390 unknown protein 30 0.85
At1g50240 hypothetical protein 28 3.2
At1g71140 hypothetical protein 27 5.5
At3g01670 unknown protein 27 7.2
At5g14120 nodulin-like protein 27 9.4
At2g21050 AUX1-like amino acid permease 27 9.4
At1g77690 AUX1-like permease like protein 27 9.4
>At2g15970 similar to cold acclimation protein WCOR413 [Triticum
aestivum]
Length = 197
Score = 258 bits (659), Expect = 1e-69
Identities = 122/182 (67%), Positives = 151/182 (82%), Gaps = 1/182 (0%)
Query: 15 LINSDFRDLSLAATKLANHAVNLGVGFGFGASFFGLIAAIAAIYLLVLDRTNWKTNILTG 74
+I SDF +L++AA LA HA L G GFG S +A+IAAIYLLVLDRTNWKTN+LT
Sbjct: 16 MIGSDFNELTIAAKNLATHAFTL-TGLGFGTSVLEWVASIAAIYLLVLDRTNWKTNMLTS 74
Query: 75 LLIPYIFFSLPSIIFNVFRGEIGKWIALVAIVLRIFIPKHFPDWLELPAALIILIVVAPG 134
LLIPYIFFSLPS+IF +FRGEIGKWIA VA+V+++F PKH ++LELP AL++L VVAP
Sbjct: 75 LLIPYIFFSLPSLIFGIFRGEIGKWIAFVAVVVQLFFPKHAREYLELPVALVLLAVVAPN 134
Query: 135 LIANTFRDNIVGVVVCLVIACYLLQEHIRASGGFRNSFTKANGVSNSIGIIILLVYPIWA 194
LIA TFRD+ +G+ +CL I CYLLQEHIRASGGFRN+FTKANG+SN++GII L+V+P+WA
Sbjct: 135 LIAGTFRDSWIGLAICLGIGCYLLQEHIRASGGFRNAFTKANGISNTVGIICLVVFPVWA 194
Query: 195 AV 196
+
Sbjct: 195 LI 196
>At3g50830 putative cold acclimation protein
Length = 203
Score = 248 bits (633), Expect = 2e-66
Identities = 117/193 (60%), Positives = 147/193 (75%), Gaps = 1/193 (0%)
Query: 5 MNFREAEAADLINSDFRDLSLAATKLANHAVNLGVGFGFGASFFGLIAAIAAIYLLVLDR 64
M + + L+NSD +L +AA KL + LG G GFG SF +A+ AAIYLL+LDR
Sbjct: 9 MKTDDVDTVALVNSDMEELKVAAKKLFSDVSKLG-GLGFGVSFLKFLASFAAIYLLILDR 67
Query: 65 TNWKTNILTGLLIPYIFFSLPSIIFNVFRGEIGKWIALVAIVLRIFIPKHFPDWLELPAA 124
TNWKT +LT LLIPYIF SLPS+IFN G++GKWIA VA+VLR+F PKHFPDWLE+P +
Sbjct: 68 TNWKTKMLTSLLIPYIFLSLPSVIFNFLSGDVGKWIAFVAVVLRLFFPKHFPDWLEMPGS 127
Query: 125 LIILIVVAPGLIANTFRDNIVGVVVCLVIACYLLQEHIRASGGFRNSFTKANGVSNSIGI 184
LI+L+VV+P +A+ R +G V+ L I CYLLQEHIRASGGFRNSFT+ GVSN++GI
Sbjct: 128 LILLLVVSPHFLAHHIRGTWIGTVISLFIGCYLLQEHIRASGGFRNSFTQPRGVSNTLGI 187
Query: 185 IILLVYPIWAAVV 197
I+LLVYP+WA +V
Sbjct: 188 ILLLVYPVWALIV 200
>At4g37220 cold acclimation protein homolog
Length = 202
Score = 244 bits (622), Expect = 3e-65
Identities = 117/192 (60%), Positives = 146/192 (75%), Gaps = 1/192 (0%)
Query: 9 EAEAADLINSDFRDLSLAATKLANHAVNLGVGFGFGASFFGLIAAIAAIYLLVLDRTNWK 68
E AA+LINSD + AA KL LG G GFG S A+I AIYLL+LDRTNWK
Sbjct: 12 EENAANLINSDMNEFVAAAKKLVKDVGMLG-GVGFGTSVLQWAASIFAIYLLILDRTNWK 70
Query: 69 TNILTGLLIPYIFFSLPSIIFNVFRGEIGKWIALVAIVLRIFIPKHFPDWLELPAALIIL 128
T +LT LL+PYIFF+LPS+IF F G+ GKWIAL+AI++R+F PK FP+WLE+P ALI++
Sbjct: 71 TKMLTTLLVPYIFFTLPSVIFQFFSGDFGKWIALIAIIVRLFFPKEFPEWLEIPVALILI 130
Query: 129 IVVAPGLIANTFRDNIVGVVVCLVIACYLLQEHIRASGGFRNSFTKANGVSNSIGIIILL 188
+VV+P LIA T R++ VG V+CLVIACYL EHI+ASGGF+NSFT+ NG+SN+IGI+ LL
Sbjct: 131 VVVSPSLIAWTLRESWVGAVICLVIACYLFHEHIKASGGFKNSFTQKNGISNTIGIVALL 190
Query: 189 VYPIWAAVVIIF 200
VYP+W IF
Sbjct: 191 VYPVWTIFFHIF 202
>At2g23680 similar to cold acclimation protein WCOR413 [Triticum
aestivum]
Length = 189
Score = 149 bits (377), Expect = 7e-37
Identities = 74/158 (46%), Positives = 108/158 (67%), Gaps = 4/158 (2%)
Query: 47 FFGLIAAIAAIYLLVLDRTNWK--TNILTGLLIPYIFFSLPSIIFNVFRGEIGKWIALVA 104
F +A+I A++L++LD+T WK NI+ LL PY+F SLP +IF V R +GKWIAL+
Sbjct: 30 FLRWLASIVAVFLMILDQTKWKYSNNIMASLLAPYLFSSLPIVIFQVLRNGVGKWIALLT 89
Query: 105 IVLRIFIPKHFPDWLELPAALIILIVVAPGLIANTFRDNI--VGVVVCLVIACYLLQEHI 162
++LR+F+P HF + LE+P A I+LIVV P I FRD++ G VCL+ + YL+ +H
Sbjct: 90 VILRLFLPNHFHESLEIPGATILLIVVTPSDIGAIFRDDLRYTGGDVCLLTSFYLINKHT 149
Query: 163 RASGGFRNSFTKANGVSNSIGIIILLVYPIWAAVVIIF 200
+A GG +NSFT+ + V+ SI + IL VYPI ++ +F
Sbjct: 150 KACGGIKNSFTQKDKVTYSICLWILFVYPILSSFAALF 187
>At1g29395 unknown protein
Length = 225
Score = 60.1 bits (144), Expect = 8e-10
Identities = 32/108 (29%), Positives = 55/108 (50%), Gaps = 3/108 (2%)
Query: 69 TNILTGLLIPYIFFSLPSIIFNVFRGEIGKWIALVAIVLRIFIPKHFPDWLELPAALIIL 128
T I +++P PS I +GE G W A +A++ R+F FP LELP ++L
Sbjct: 102 TGIHKSVVVPLFALHAPSSIVAWIKGEYGVWAAFLALIARLFFT--FPGELELPFIALLL 159
Query: 129 IVVAPGLIANTFRDNIVGVVVCLVIACYLLQEHIRASGGFRNSFTKAN 176
++VAP + N R G ++ + I+ +L +H +G ++ K +
Sbjct: 160 VIVAPYQVMN-IRGKQEGAIIAIAISGFLAFQHFSRAGSLEKAYEKGS 206
>At1g29390 unknown protein
Length = 226
Score = 60.1 bits (144), Expect = 8e-10
Identities = 36/117 (30%), Positives = 61/117 (51%), Gaps = 4/117 (3%)
Query: 69 TNILTGLLIPYIFFSLPSIIFNVFRGEIGKWIALVAIVLRIFIPKHFPDWLELPAALIIL 128
T I ++P P I + +GE G W A +A++ R+F FP LELP ++L
Sbjct: 103 TGIHKSFVVPLFALQAPMGIVSWMKGEYGIWAAFLALLTRLFF--SFPVELELPFIALLL 160
Query: 129 IVVAPGLIANTFRDNIVGVVVCLVIACYLLQEHIRASGGFRNSFTKANGVSNSIGII 185
++VAP + + R G ++ L I+C+L +H +G + +F + N V ++ II
Sbjct: 161 VIVAPYQVM-SIRGKQEGAILSLAISCFLAFQHFSRAGTLQKAFDQ-NSVLATVAII 215
>At4g29140 unknown protein
Length = 532
Score = 31.6 bits (70), Expect = 0.29
Identities = 19/86 (22%), Positives = 35/86 (40%)
Query: 79 YIFFSLPSIIFNVFRGEIGKWIALVAIVLRIFIPKHFPDWLELPAALIILIVVAPGLIAN 138
Y+ FSLP ++ N I ++ I+ + + LPA L ++ + GL
Sbjct: 179 YLIFSLPDLLTNTLLHPIRIYLRAQGIIHPVTLASLSGAVFHLPANLFLVSYLRLGLTGV 238
Query: 139 TFRDNIVGVVVCLVIACYLLQEHIRA 164
+I + V + CY+ + A
Sbjct: 239 AVASSITNIFVVAFLVCYVWASGLHA 264
>At5g13390 unknown protein
Length = 1126
Score = 30.0 bits (66), Expect = 0.85
Identities = 26/79 (32%), Positives = 37/79 (45%), Gaps = 7/79 (8%)
Query: 56 AIYLLVLDRTNWKTNILTGLLIPYIFFSLPSIIFNV-FRGEIGKWIALVAIVLRIFIPKH 114
A +LL L T + GLLI YI SL NV F G +G W++L+A + F
Sbjct: 75 AAFLLDLGGTPVVATLTIGLLISYIVDSL-----NVKFGGFLGIWMSLLAAQISFFFSSS 129
Query: 115 -FPDWLELPAALIILIVVA 132
F + +P L+ + A
Sbjct: 130 LFSSFNSVPLGLLAAFLCA 148
>At1g50240 hypothetical protein
Length = 1008
Score = 28.1 bits (61), Expect = 3.2
Identities = 18/57 (31%), Positives = 27/57 (46%), Gaps = 1/57 (1%)
Query: 139 TFRDNIVGVVVCLVIACYLLQEHIRASGGFRNSFTKANGVSNSIGIII-LLVYPIWA 194
TFR +V + +I C L HI+ + +GV +I +II LL +P A
Sbjct: 613 TFRQVLVKIETMKLITCLLSDAHIKLVKSWGGPGGGKDGVRETINVIIDLLAFPFVA 669
>At1g71140 hypothetical protein
Length = 485
Score = 27.3 bits (59), Expect = 5.5
Identities = 19/51 (37%), Positives = 22/51 (42%)
Query: 9 EAEAADLINSDFRDLSLAATKLANHAVNLGVGFGFGASFFGLIAAIAAIYL 59
E E D + S LSL+ A HA GV G G G +AA YL
Sbjct: 362 ETEVVDYVKSMAPLLSLSVIFDALHAALSGVARGSGRQDIGAYVNLAAYYL 412
>At3g01670 unknown protein
Length = 846
Score = 26.9 bits (58), Expect = 7.2
Identities = 14/53 (26%), Positives = 24/53 (44%)
Query: 117 DWLELPAALIILIVVAPGLIANTFRDNIVGVVVCLVIACYLLQEHIRASGGFR 169
D ++L +I + + P I F D+I V +V + HI + GF+
Sbjct: 337 DMVDLTTTIIDIYQLPPNHITAAFTDHIPTAVYWIVRCVLICVSHISGASGFK 389
>At5g14120 nodulin-like protein
Length = 579
Score = 26.6 bits (57), Expect = 9.4
Identities = 38/152 (25%), Positives = 66/152 (43%), Gaps = 24/152 (15%)
Query: 51 IAAIAAIYLLVLDRTNWKTNILTGLLIPYI--FFSLPSIIFNVFRGEIGKWIALVAIVLR 108
I + A+ +L+ N +T TG L+ + F + + +G G A+++ +
Sbjct: 108 ILPLWAMCVLIFVGNNGETYFNTGALVSGVQNFPKSRGPVVGILKGFAGLGGAIIS---Q 164
Query: 109 IFIPKHFPDWLELPAALIILIVVAPGLIANTFRDNIVGVVVCLVIACYLLQEH--IRASG 166
I+ H + PA+LI+++ V P + VVVCL+ + H IR +
Sbjct: 165 IYTMIHSSN----PASLILMVAVTPAV-----------VVVCLMFFIRPVGGHKQIRPTD 209
Query: 167 GFRNSFTKANGVSNSIGIIILLVYPIWAAVVI 198
G SFT GV + ++ V I VV+
Sbjct: 210 G--ASFTFIYGVCLLLAAYLMSVMLIQDLVVV 239
>At2g21050 AUX1-like amino acid permease
Length = 483
Score = 26.6 bits (57), Expect = 9.4
Identities = 13/38 (34%), Positives = 20/38 (52%), Gaps = 3/38 (7%)
Query: 75 LLIPYIFFSL---PSIIFNVFRGEIGKWIALVAIVLRI 109
L +PY F L I+F +F G +G W A + +L +
Sbjct: 59 LTLPYSFSQLGMLSGILFQLFYGILGSWTAYLISILYV 96
>At1g77690 AUX1-like permease like protein
Length = 470
Score = 26.6 bits (57), Expect = 9.4
Identities = 14/38 (36%), Positives = 20/38 (51%), Gaps = 3/38 (7%)
Query: 75 LLIPYIFFSL---PSIIFNVFRGEIGKWIALVAIVLRI 109
L +PY F L I+F +F G +G W A + VL +
Sbjct: 63 LTLPYSFSQLGMMSGILFQLFYGLMGSWTAYLISVLYV 100
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.331 0.147 0.455
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,168,302
Number of Sequences: 26719
Number of extensions: 161933
Number of successful extensions: 553
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 539
Number of HSP's gapped (non-prelim): 15
length of query: 200
length of database: 11,318,596
effective HSP length: 94
effective length of query: 106
effective length of database: 8,807,010
effective search space: 933543060
effective search space used: 933543060
T: 11
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0091b.8