BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0082b.1
(122 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g08030 glycerophosphodiester phosphodiesterase - like protein 45 7e-06
At1g74210 glycerophosphodiester phosphodiesterase like protein 39 6e-04
At4g08880 hypothetical protein 33 0.026
At1g05950 hypothetical protein 30 0.29
At4g21360 putative transposable element 28 1.4
At3g50480 putative protein 28 1.4
At2g05560 hypothetical protein 27 1.9
At5g35010 putative protein 27 3.2
At5g01570 putative protein 27 3.2
At2g14770 hypothetical protein 27 3.2
At4g05290 putative protein 26 4.1
At3g43390 putative protein 26 4.1
At3g42740 putative protein 26 4.1
At2g45040 metalloproteinase like protein ,predicted GPI-anchored... 26 4.1
At3g04730 auxin-induced protein 25 7.1
At1g56050 like GTP-binding protein 25 7.1
At5g54470 putative protein 25 9.2
At3g11020 DREB2B transcription factor 25 9.2
At1g57620 unknown protein 25 9.2
>At5g08030 glycerophosphodiester phosphodiesterase - like protein
Length = 372
Score = 45.4 bits (106), Expect = 7e-06
Identities = 35/116 (30%), Positives = 52/116 (44%), Gaps = 15/116 (12%)
Query: 17 IGIYPEIKAPWFHKQ-----EGKDISSKVLAVLKQYGYTGK-------NDNVYLQCFDAN 64
+GIYPEIK P F Q +GK K + LK+YGY G +++Q F A
Sbjct: 173 VGIYPEIKNPVFMNQQVKWADGKKFEDKFVETLKKYGYKGSYLSEDWLKQPIFIQSFAAT 232
Query: 65 ELKRIKNELE-PKLGMDLKLVQLIAYNDWQETYEQKADGKWVEYDYDWMFKPGAMK 119
L I N + PKL + + I D +TY + +++Y ++ G K
Sbjct: 233 SLVYISNMTDSPKLFLIDDVT--ILTEDTNKTYAEITSDAYLDYIKPYVIGIGPWK 286
>At1g74210 glycerophosphodiester phosphodiesterase like protein
Length = 392
Score = 38.9 bits (89), Expect = 6e-04
Identities = 32/115 (27%), Positives = 47/115 (40%), Gaps = 13/115 (11%)
Query: 17 IGIYPEIKAPWFHKQE-----GKDISSKVLAVLKQYGYTGK-------NDNVYLQCFDAN 64
+GIYPEIK P Q GK KV+ LK+YGY G +++Q F
Sbjct: 173 VGIYPEIKNPVLMNQHVKWPGGKKFEDKVVETLKKYGYGGSYLSKKWLKKPLFIQSFAPT 232
Query: 65 ELKRIKNELEPKLGMDLKLVQLIAYNDWQETYEQKADGKWVEYDYDWMFKPGAMK 119
L I N + + + V + D +TY + + EY ++ G K
Sbjct: 233 SLVYISNLTDSPKVLLIDDVTM-PTQDTNQTYAEITSDAYFEYIKQYVVGIGPWK 286
>At4g08880 hypothetical protein
Length = 1175
Score = 33.5 bits (75), Expect = 0.026
Identities = 17/44 (38%), Positives = 24/44 (53%), Gaps = 4/44 (9%)
Query: 28 FHKQEGKDISSKVLAVLKQYG----YTGKNDNVYLQCFDANELK 67
F +E +DI + L +K+YG YT N YL+C D N L+
Sbjct: 1097 FESEEARDICEEALEDIKKYGNTLKYTYANSFSYLECGDVNTLE 1140
>At1g05950 hypothetical protein
Length = 590
Score = 30.0 bits (66), Expect = 0.29
Identities = 13/49 (26%), Positives = 25/49 (50%)
Query: 34 KDISSKVLAVLKQYGYTGKNDNVYLQCFDANELKRIKNELEPKLGMDLK 82
+ ++ +V AV+ Y Y + DN +L+C + + ++P L LK
Sbjct: 50 ESVAKQVHAVVLLYNYYHRKDNPHLECLSFESFRSLATVMKPALLQHLK 98
>At4g21360 putative transposable element
Length = 1308
Score = 27.7 bits (60), Expect = 1.4
Identities = 20/79 (25%), Positives = 36/79 (45%), Gaps = 26/79 (32%)
Query: 27 WFHKQEGKDISSKVLAVLKQYGYTGKNDNVYLQCFDANELKRIKNELEPKLGMDLKLVQL 86
WF K+EG +K+ Y GK ++++NE + K G+ + +
Sbjct: 258 WFCKKEGH---------VKKDCYAGK--------------RKLENEGQGKAGV---ITEK 291
Query: 87 IAYNDWQETYEQKADGKWV 105
+ Y++ Y+Q+A KWV
Sbjct: 292 LVYSEALSMYDQEAKDKWV 310
>At3g50480 putative protein
Length = 200
Score = 27.7 bits (60), Expect = 1.4
Identities = 16/58 (27%), Positives = 29/58 (49%), Gaps = 6/58 (10%)
Query: 43 VLKQYGYTGKNDNVYLQCFDANELKRIKNELEPKLGMDLKLVQLIAYNDWQETYEQKA 100
V ++Y Y K +++ L EL R+ E++ D+K ++ I + W + E KA
Sbjct: 99 VCRKYRYIKKLESIEL------ELVRVAREIQVHQWTDIKEMKAIQVHQWTDIKEMKA 150
>At2g05560 hypothetical protein
Length = 1471
Score = 27.3 bits (59), Expect = 1.9
Identities = 14/36 (38%), Positives = 21/36 (57%), Gaps = 4/36 (11%)
Query: 31 QEGKDISSKVLAVLKQYG----YTGKNDNVYLQCFD 62
+E +DI +VL +K+YG YT N +Y +C D
Sbjct: 1371 EEARDICEEVLEDIKKYGNTLKYTYANSFLYPECGD 1406
>At5g35010 putative protein
Length = 230
Score = 26.6 bits (57), Expect = 3.2
Identities = 15/41 (36%), Positives = 22/41 (53%), Gaps = 4/41 (9%)
Query: 31 QEGKDISSKVLAVLKQYG----YTGKNDNVYLQCFDANELK 67
+E +DI +VL +K+YG YT N Y +C D + K
Sbjct: 158 EEARDICEEVLEDIKKYGNTLKYTYANGFSYPECGDVSTPK 198
>At5g01570 putative protein
Length = 157
Score = 26.6 bits (57), Expect = 3.2
Identities = 11/38 (28%), Positives = 22/38 (56%)
Query: 52 KNDNVYLQCFDANELKRIKNELEPKLGMDLKLVQLIAY 89
K D + D E++R++ EL+ +L ++ KL + + Y
Sbjct: 69 KTDEANSEIADEEEIERLQKELDEELELECKLKEELRY 106
>At2g14770 hypothetical protein
Length = 1756
Score = 26.6 bits (57), Expect = 3.2
Identities = 14/38 (36%), Positives = 21/38 (54%), Gaps = 4/38 (10%)
Query: 31 QEGKDISSKVLAVLKQYG----YTGKNDNVYLQCFDAN 64
+E +DI +VL +K+YG YT N Y +C D +
Sbjct: 1502 EEARDICEEVLEDIKKYGNTLKYTYANSFSYPECGDTS 1539
>At4g05290 putative protein
Length = 213
Score = 26.2 bits (56), Expect = 4.1
Identities = 14/36 (38%), Positives = 20/36 (54%), Gaps = 4/36 (11%)
Query: 31 QEGKDISSKVLAVLKQYG----YTGKNDNVYLQCFD 62
+E +DI +VL +K+YG YT N Y +C D
Sbjct: 141 EEARDICEEVLEDIKKYGNTLKYTYANSFSYPECGD 176
>At3g43390 putative protein
Length = 1113
Score = 26.2 bits (56), Expect = 4.1
Identities = 14/38 (36%), Positives = 21/38 (54%), Gaps = 4/38 (10%)
Query: 31 QEGKDISSKVLAVLKQYG----YTGKNDNVYLQCFDAN 64
+E +DI +VL +K+YG YT N Y +C D +
Sbjct: 1041 EEARDICEEVLEDIKKYGNTLKYTYANSFSYPKCGDVS 1078
>At3g42740 putative protein
Length = 230
Score = 26.2 bits (56), Expect = 4.1
Identities = 14/41 (34%), Positives = 22/41 (53%), Gaps = 4/41 (9%)
Query: 31 QEGKDISSKVLAVLKQYG----YTGKNDNVYLQCFDANELK 67
+E +DI +VL +K+YG YT N + +C D L+
Sbjct: 158 EEARDICEEVLEDIKKYGNTLKYTYANSFSFPECGDVRTLE 198
>At2g45040 metalloproteinase like protein ,predicted GPI-anchored
protein (by homology)
Length = 342
Score = 26.2 bits (56), Expect = 4.1
Identities = 19/64 (29%), Positives = 28/64 (43%), Gaps = 6/64 (9%)
Query: 2 EIEFVQGLNHSTGKNIGIYPEIKAPWFHKQEGKDISSKVLAVLKQYGYTGKNDNVYLQCF 61
EI V GL HS+ K+ +YP + K K ++ + V+ G N N L
Sbjct: 253 EIGHVLGLGHSSVKDAAMYPTL------KPRSKKVNLNMDDVVGVQSLYGTNPNFTLNSL 306
Query: 62 DANE 65
A+E
Sbjct: 307 LASE 310
>At3g04730 auxin-induced protein
Length = 236
Score = 25.4 bits (54), Expect = 7.1
Identities = 15/41 (36%), Positives = 22/41 (53%), Gaps = 2/41 (4%)
Query: 82 KLVQLIAYNDWQETYEQKADGKWVEY-DYDWMFKPGAMKKI 121
KL+ L+ +D+ TYE K DG W+ D W + K+I
Sbjct: 174 KLIDLLNGSDYVPTYEDK-DGDWMLVGDVPWEMFVDSCKRI 213
>At1g56050 like GTP-binding protein
Length = 421
Score = 25.4 bits (54), Expect = 7.1
Identities = 12/58 (20%), Positives = 35/58 (59%), Gaps = 8/58 (13%)
Query: 32 EGKDISSKVLAVLKQYGYTGKNDNVY--LQCFDANELKRIKNELEPKLGMDLKLVQLI 87
+G+ + +K L+ +++ D++ ++CF+ N++ + +++PK +D+ ++LI
Sbjct: 134 QGEGLGNKFLSHIREV------DSILQVVRCFEDNDIIHVNGKVDPKSDIDVINLELI 185
>At5g54470 putative protein
Length = 229
Score = 25.0 bits (53), Expect = 9.2
Identities = 17/58 (29%), Positives = 28/58 (47%), Gaps = 3/58 (5%)
Query: 66 LKRIKNELEPKLGMDLKLVQ---LIAYNDWQETYEQKADGKWVEYDYDWMFKPGAMKK 120
L R KN G D L Q +I ND E+Y++++D E + + P A+++
Sbjct: 75 LARKKNNNSSLAGRDQNLNQEEEIIGCNDGAESYDEESDEDEEEEEVENQVVPAAVEQ 132
>At3g11020 DREB2B transcription factor
Length = 330
Score = 25.0 bits (53), Expect = 9.2
Identities = 19/57 (33%), Positives = 23/57 (40%), Gaps = 7/57 (12%)
Query: 41 LAVLKQYGYTGKNDNVYLQ-------CFDANELKRIKNELEPKLGMDLKLVQLIAYN 90
L + YG+ ND V Q CFD NEL NE P D V +Y+
Sbjct: 252 LLTVADYGWPWSNDIVNDQTSWDPNECFDINELLGDLNEPGPHQSQDQNHVNSGSYD 308
>At1g57620 unknown protein
Length = 212
Score = 25.0 bits (53), Expect = 9.2
Identities = 15/63 (23%), Positives = 29/63 (45%), Gaps = 5/63 (7%)
Query: 46 QYGYTGKNDNVYLQCFDANELKRIKNELEPKLGMDLKLVQLIAYNDWQE-TYEQKADGKW 104
Q+ +T + YL CF+A+ K+ +++ IA DW ++K +G
Sbjct: 87 QFAFTTQESGTYLACFEAD----AKSHGNKDFSINIDWKTGIAAKDWDSIARKEKIEGVE 142
Query: 105 VEY 107
+E+
Sbjct: 143 LEF 145
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.315 0.137 0.418
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,023,248
Number of Sequences: 26719
Number of extensions: 119634
Number of successful extensions: 184
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 173
Number of HSP's gapped (non-prelim): 21
length of query: 122
length of database: 11,318,596
effective HSP length: 87
effective length of query: 35
effective length of database: 8,994,043
effective search space: 314791505
effective search space used: 314791505
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 53 (25.0 bits)
Lotus: description of TM0082b.1