BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0081b.3
(322 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g48070 unknown protein 219 2e-57
At5g62910 unknown protein 191 6e-49
At1g74870 hypothetical protein 108 3e-24
At2g28530 putative RING zinc finger transcription negative regul... 60 2e-09
At3g45630 unknown protein 59 4e-09
At5g56340 unknown protein (At5g56340) 39 0.005
At5g41350 unknown protein 37 0.011
At4g39350 cellulose synthase catalytic subunit (Ath-A) 37 0.014
At1g27010 putative protein 37 0.014
At4g02760 hypothetical protein 37 0.019
At4g00335 RING-H2 finger protein RHB1a 37 0.019
At3g03050 putative cellulose synthase catalytic subunit 37 0.019
At4g01020 unknown protein 36 0.025
At3g13150 hypothetical protein 36 0.025
At5g60130 putative protein 36 0.032
At4g26400 unknown protein 36 0.032
At4g37820 unknown protein 35 0.042
At3g13780 hypothetical protein 35 0.042
At2g39100 RING zinc finger like protein 35 0.042
At1g47900 mysoin-like protein 35 0.042
>At3g48070 unknown protein
Length = 319
Score = 219 bits (558), Expect = 2e-57
Identities = 137/289 (47%), Positives = 175/289 (60%), Gaps = 26/289 (8%)
Query: 13 KKKRTNGSAKLKQIKLDVRREQWLSRVKKGCNVDSNGRMDNCPSSKHTASE--ENRSSCK 70
KKKRTN SAKLKQ KL +RREQWLS+V + + G + S++ SE + R +
Sbjct: 22 KKKRTNRSAKLKQSKLGLRREQWLSQVA----MINKGDKEEMESNRRIGSEKPDQRDLPR 77
Query: 71 EMTRKGEDIEGTCIQDSDSRSTINSPDRSTYSHDESSGKDFSGSSSNSISTSCSGNDSEE 130
+ ED GT +S S NSP+ + +S +FS SSS+ SCSGN +EE
Sbjct: 78 PVENLDEDNNGTHRHESFIESLSNSPN--SILSGMNSIPNFSSSSSSGSGGSCSGNITEE 135
Query: 131 ED--DDGCLDDWEAVADALYANDKSHSVVS--ESPAEHEAECRYTVLEDDKNPRVDFSKA 186
ED DDGCLDDWEA+ADAL A+D+ H + ES EHE + + + L + + +KA
Sbjct: 136 EDADDDGCLDDWEAIADALAADDEKHEKENPPESCQEHE-DIKQSALIGGADVVLRDAKA 194
Query: 187 DFKSEVPESKPNRRAWKPDDTLRPRCLPNLSKQRNSPLNSNWRGSRKTVPWAWQTIISQP 246
D S+ + K N+ AW+PDD LRP+ LPNL KQR+ P+ N S TV P
Sbjct: 195 D--SQRRKQKSNQ-AWRPDDKLRPQGLPNLEKQRSFPV-MNLHFSSVTVV---------P 241
Query: 247 SQCPICYEDLDVTDSSFLPCSCGFHLCLFCHKKILEADARCPSCRKLYD 295
S CPICYEDLD+TDS+FLPC CGF LCLFCHK I + D RCP CRK Y+
Sbjct: 242 SSCPICYEDLDLTDSNFLPCPCGFRLCLFCHKTICDGDGRCPGCRKPYE 290
>At5g62910 unknown protein
Length = 327
Score = 191 bits (484), Expect = 6e-49
Identities = 124/301 (41%), Positives = 153/301 (50%), Gaps = 40/301 (13%)
Query: 13 KKKRTNGSAKLKQIKLDVRREQWLSRVKKGCNVDSNGRMDNCPSSKHTASEENRSSCKEM 72
KKKR N SAK+KQ KL +RREQWLS+V R N H S +
Sbjct: 20 KKKRNNKSAKMKQNKLGLRREQWLSQVAVSNKEVKEERSVNRSQKPHHESSDK------- 72
Query: 73 TRKGEDIEG---TCIQDSDSRSTINSPDRSTYSHDESSGKDFSGSSSNSISTSCSGNDSE 129
R+ ED G +S S NS TYS SG+ S SS+S S CSGN +E
Sbjct: 73 VRREEDNNGGNNLLHHESFMESPSNSSVGGTYSSTNFSGR--SSRSSSSSSGFCSGNITE 130
Query: 130 EE----DDDGCLDDWEAVADALYANDKSHSVVSESPAEHEAE-----------CRYTVLE 174
EE DDDGC+DDWEAVADAL A ++ P E E C ++ +
Sbjct: 131 EENVDDDDDGCVDDWEAVADALAAEEEIEK--KSRPLESVKEQVSVGQSASNVCDSSISD 188
Query: 175 DDKNPRVDFSKADFKSEVPESKPNRRAWKPDDTLRPRCLPNLSKQRNSPLNSNWRGSRKT 234
V+ K + + + RAW+ DD LRP+ LPNL+KQ + P S
Sbjct: 189 ASDVVGVEDPKQECLRVSSRKQTSNRAWRLDDDLRPQGLPNLAKQLSFPELDKRFSS--- 245
Query: 235 VPWAWQTIISQPSQCPICYEDLDVTDSSFLPCSCGFHLCLFCHKKILEADARCPSCRKLY 294
++ PS CPICYEDLD+TDS+FLPC CGF LCLFCHK I + D RCP CRK Y
Sbjct: 246 --------VAIPSSCPICYEDLDLTDSNFLPCPCGFRLCLFCHKTICDGDGRCPGCRKPY 297
Query: 295 D 295
+
Sbjct: 298 E 298
>At1g74870 hypothetical protein
Length = 289
Score = 108 bits (271), Expect = 3e-24
Identities = 90/289 (31%), Positives = 119/289 (41%), Gaps = 57/289 (19%)
Query: 12 SKKKRTNGSAKLKQIKLDVRREQWLSRVKKGCNVDSN--GRMDNCPSSKHTASEENRSSC 69
SKK++ N KLKQ K+D RR+QW+S+ KK NVD GR K T + R
Sbjct: 20 SKKRKANWFCKLKQWKIDARRKQWISQWKKA-NVDEEEIGRRLRSLLEKLTDQKAWRIDY 78
Query: 70 KEMTRKGEDIEGTCIQDSDSRSTINSPDRSTYSHDESSGKDFSGSSSNSISTSCSGNDSE 129
+ D+E T S +SP D SG F CS +E
Sbjct: 79 DDDEDDEIDLERTS-------SFASSPTSVLKRKDSVSGDCFC----------CSKQMTE 121
Query: 130 EEDD--DGCLDDWEAVADALYANDKSHSVVSESPAEHEAECRYTVLEDDKNPRVDFSKAD 187
EE++ D D+W+ DAL + E+D N D
Sbjct: 122 EEEEVFDDAYDNWDGFKDALNS-----------------------FENDNNESSRLVTED 158
Query: 188 FKSEVPESKPN--RRAWKPDDTLRPRCLPNLSKQRNSPLNSNWRGSRKTVPWAWQTIISQ 245
F+ E + P+ +R K P + + N SN R
Sbjct: 159 FEQEEEDLIPDTSQRMNKCKQEAAPGNQTTIHRNSNKKKRSNSEKQRG----------DG 208
Query: 246 PSQCPICYEDLDVTDSSFLPCSCGFHLCLFCHKKILEADARCPSCRKLY 294
+CPIC E +D TD F PC+CGF +CLFCH KI E +ARCP+CRK Y
Sbjct: 209 DEECPICSELMDATDLEFEPCTCGFRICLFCHNKISENEARCPACRKDY 257
>At2g28530 putative RING zinc finger transcription negative
regulator protein
Length = 236
Score = 59.7 bits (143), Expect = 2e-09
Identities = 22/53 (41%), Positives = 33/53 (61%), Gaps = 6/53 (11%)
Query: 249 CPICYEDLDVTDSSFLPCSCGFHLCLFCHKKILE------ADARCPSCRKLYD 295
CP+C E++D+TD PC CG+ +C++C I+E + RCP+CR YD
Sbjct: 9 CPLCTEEMDLTDQHLKPCKCGYQICVWCWHHIIEMAEKDKTEGRCPACRTRYD 61
>At3g45630 unknown protein
Length = 989
Score = 58.9 bits (141), Expect = 4e-09
Identities = 21/53 (39%), Positives = 34/53 (63%), Gaps = 6/53 (11%)
Query: 249 CPICYEDLDVTDSSFLPCSCGFHLCLFCHKKIL------EADARCPSCRKLYD 295
CP+C E++D+TD PC CG+ +C++C I+ +++ RCP+CR YD
Sbjct: 9 CPLCAEEMDLTDQQLKPCKCGYQICVWCWHHIMDMAEKDQSEGRCPACRTPYD 61
>At5g56340 unknown protein (At5g56340)
Length = 396
Score = 38.5 bits (88), Expect = 0.005
Identities = 24/73 (32%), Positives = 35/73 (47%), Gaps = 4/73 (5%)
Query: 221 NSPLNSNWRGSRKTVPWAWQTI-ISQPSQCPICYEDLDV-TDSSFLPCSCGFHLCLFCHK 278
N P +RK A T+ I +P QC +C +D + T++ +PC FH + C
Sbjct: 230 NDPNRQGTPPARKEAVEALPTVKIMEPLQCSVCLDDFEKGTEAKEMPCKHKFH--VRCIV 287
Query: 279 KILEADARCPSCR 291
LE + CP CR
Sbjct: 288 PWLELHSSCPVCR 300
>At5g41350 unknown protein
Length = 212
Score = 37.4 bits (85), Expect = 0.011
Identities = 17/50 (34%), Positives = 23/50 (46%), Gaps = 1/50 (2%)
Query: 243 ISQPSQCPICYEDLDVTDSSFLPCSCGFHLCLFCHKKILEADARCPSCRK 292
I + CPIC E+ D+ + L C H L C + +E CP C K
Sbjct: 155 IEEEEDCPICLEEYDIENPK-LVAKCDHHFHLACILEWMERSETCPVCNK 203
>At4g39350 cellulose synthase catalytic subunit (Ath-A)
Length = 1084
Score = 37.0 bits (84), Expect = 0.014
Identities = 18/56 (32%), Positives = 33/56 (58%), Gaps = 4/56 (7%)
Query: 249 CPICYEDLDVTDSS--FLPCS-CGFHLCLFCHK-KILEADARCPSCRKLYDHVDGN 300
C IC +++++T SS F+ C+ C F +C C++ + E + CP C+ Y + G+
Sbjct: 39 CQICGDEIELTVSSELFVACNECAFPVCRPCYEYERREGNQACPQCKTRYKRIKGS 94
>At1g27010 putative protein
Length = 125
Score = 37.0 bits (84), Expect = 0.014
Identities = 18/45 (40%), Positives = 23/45 (51%)
Query: 247 SQCPICYEDLDVTDSSFLPCSCGFHLCLFCHKKILEADARCPSCR 291
S+C IC ED V+D + CG FC K + + CPSCR
Sbjct: 65 SRCVICLEDYMVSDIVRVLPHCGHQFHAFCIDKWFQLCSTCPSCR 109
>At4g02760 hypothetical protein
Length = 419
Score = 36.6 bits (83), Expect = 0.019
Identities = 21/47 (44%), Positives = 28/47 (58%), Gaps = 1/47 (2%)
Query: 87 SDSRSTINSPDRSTYSHDESSGKDFSGSSSNSISTSCSGNDSEEEDD 133
S S S + SP S+ S D SS D S SSS+ S+ SG + EE++D
Sbjct: 374 SPSESDVRSPSPSS-SSDSSSSSDSSSSSSSGESSDESGTEEEEDED 419
>At4g00335 RING-H2 finger protein RHB1a
Length = 190
Score = 36.6 bits (83), Expect = 0.019
Identities = 18/44 (40%), Positives = 23/44 (51%), Gaps = 1/44 (2%)
Query: 249 CPICYEDLDVTDSSFLPCSCGFHLCLFCHKKILEADARCPSCRK 292
CPIC+ED DV ++ L C L C + +E RCP C K
Sbjct: 139 CPICFEDYDV-ENPRLTTKCEHEFHLSCLLEWIERSDRCPICDK 181
>At3g03050 putative cellulose synthase catalytic subunit
Length = 1145
Score = 36.6 bits (83), Expect = 0.019
Identities = 13/42 (30%), Positives = 19/42 (44%)
Query: 257 DVTDSSFLPCSCGFHLCLFCHKKILEADARCPSCRKLYDHVD 298
D LPC C F +C C ++ CP C++ Y + D
Sbjct: 142 DERGQDLLPCECDFKICRDCFMDAVKTGGMCPGCKEPYRNTD 183
>At4g01020 unknown protein
Length = 1787
Score = 36.2 bits (82), Expect = 0.025
Identities = 18/57 (31%), Positives = 27/57 (46%), Gaps = 5/57 (8%)
Query: 248 QCPICYEDLDVTDS-SFLPCSCGFHLCLFCHKKILEADARCPSCRKLYDHVDGNVGL 303
+CPIC ++ TD + L C CG H+C C L+ + C + G +GL
Sbjct: 1718 ECPICKSTIEKTDGCNHLQCRCGKHICWTC----LDVFTQAEPCYAHLRTIHGGIGL 1770
>At3g13150 hypothetical protein
Length = 551
Score = 36.2 bits (82), Expect = 0.025
Identities = 30/98 (30%), Positives = 46/98 (46%), Gaps = 9/98 (9%)
Query: 46 DSNGRMDNCP--SSKHTASEENRSSCKEMTRKGEDIEGTCIQDSDSRSTINSPDRSTYSH 103
DS+ + + P SS ++S ++ SS + + S SR +SPD YS+
Sbjct: 459 DSSSSVSSSPDSSSSVSSSPDSYSSFSSSPDSSSSVSSSLF--SSSRENSSSPD---YSN 513
Query: 104 DESSGKDFSG--SSSNSISTSCSGNDSEEEDDDGCLDD 139
SS D+SG SSS+ S+S + + G LDD
Sbjct: 514 SVSSSLDYSGSVSSSSDYSSSVFPSANSSSSSSGLLDD 551
>At5g60130 putative protein
Length = 300
Score = 35.8 bits (81), Expect = 0.032
Identities = 30/126 (23%), Positives = 55/126 (42%), Gaps = 7/126 (5%)
Query: 59 HTASEENRSSCKEMTRKGEDIEGTCIQ-DSDSRSTINSPDRSTYSHDESSGKDFSGSSSN 117
H E+R+ CK++ R + E ++ DSD ++ + S D+ S D++ N
Sbjct: 77 HFCIYEHRTMCKKIRRSSDQSEEIKVESDSDEQNQASDDVLSLDEDDDDS--DYNCGEDN 134
Query: 118 SISTSCSGNDSEEEDDDGCLDDWEAVADALYANDKSHSVVSES----PAEHEAECRYTVL 173
E++D+D +D + VAD + D + +S A+ + E L
Sbjct: 135 DSDDYADEAAVEKDDNDADDEDVDNVADDVPVEDDDYVEAFDSRDHAKADDDDEDERQYL 194
Query: 174 EDDKNP 179
+D +NP
Sbjct: 195 DDRENP 200
>At4g26400 unknown protein
Length = 356
Score = 35.8 bits (81), Expect = 0.032
Identities = 19/50 (38%), Positives = 27/50 (54%), Gaps = 3/50 (6%)
Query: 243 ISQPSQCPICYEDLDV-TDSSFLPCSCGFHLCLFCHKKILEADARCPSCR 291
IS+ QC IC +D D +++ +PC FH + C LE + CP CR
Sbjct: 235 ISESLQCSICLDDFDKGSEAKEMPCKHKFH--IRCIVPWLELHSSCPVCR 282
>At4g37820 unknown protein
Length = 532
Score = 35.4 bits (80), Expect = 0.042
Identities = 29/128 (22%), Positives = 53/128 (40%), Gaps = 14/128 (10%)
Query: 8 DPPLSKKKRTNGSAKLKQIKLDVRREQWLSRVKKGCNVDSNGRMDNCPSSKHTASEENRS 67
+ P +K+K + S + +IK +E+ S ++G +K T + + S
Sbjct: 370 EEPENKEKEASSSQEENEIKETEIKEKEESSSQEG------------NENKETEKKSSES 417
Query: 68 SCKEMTRKGEDIEGTCIQDSDSRSTINSPDRSTYSHDESSGKDFSGSSSNSISTSCSGND 127
KE T + IE ++ +DS +T ++ T SG D S + S+
Sbjct: 418 QRKENTNSEKKIEQ--VESTDSSNTQKGDEQKTDESKRESGNDTSNKETEDDSSKTESEK 475
Query: 128 SEEEDDDG 135
EE + +G
Sbjct: 476 KEENNRNG 483
Score = 32.7 bits (73), Expect = 0.27
Identities = 46/204 (22%), Positives = 74/204 (35%), Gaps = 44/204 (21%)
Query: 37 SRVKKGCNVDSNGRMDNCPSSKHTASEENRSSCKEMTRKGEDIEGTCIQDSDSRSTINSP 96
S V G SN +++ SK ++E E K E +EG+ I++ +T N
Sbjct: 172 SEVMHGTEEKSNEKVEVEGESKSNSTENVSVHEDESGPKNEVLEGSVIKEVSLNTTENGS 231
Query: 97 D----RSTYSH------------------------------DESSGKDFSGSSSNSISTS 122
D + T S ESSG D SGSS S
Sbjct: 232 DDGEQQETKSELDSKTGEKGFSDSNGELPETNLSTSNATETTESSGSDESGSSGKSTGYQ 291
Query: 123 CSGNDSEE-------EDDDGCLDDWEAVADALYANDKSHSVVSESPAEHEAECRYTVLED 175
+ N+ +E E++ + + DA + D+S E + E+ + E+
Sbjct: 292 QTKNEEDEKEKVQSSEEESKVKESGKNEKDASSSQDESKEEKPERKKKEESSSQGEGKEE 351
Query: 176 DKNPRV---DFSKADFKSEVPESK 196
+ R S+ + K E PE+K
Sbjct: 352 EPEKREKEDSSSQEESKEEEPENK 375
Score = 30.0 bits (66), Expect = 1.8
Identities = 21/104 (20%), Positives = 42/104 (40%), Gaps = 8/104 (7%)
Query: 40 KKGCNVDSNGRMDNCPSSKHTASEENRSSCKEMTRKGEDIEGTCIQDSDSRST----INS 95
++G +NG + +E + +E+ KG+D + ++ +S +
Sbjct: 131 EEGNEDSNNGESEKVVDESEGGNEISNEEAREINYKGDDASSEVMHGTEEKSNEKVEVEG 190
Query: 96 PDRSTYSHDESSGKDFSGSSS----NSISTSCSGNDSEEEDDDG 135
+S + + S +D SG + S+ S N +E DDG
Sbjct: 191 ESKSNSTENVSVHEDESGPKNEVLEGSVIKEVSLNTTENGSDDG 234
>At3g13780 hypothetical protein
Length = 296
Score = 35.4 bits (80), Expect = 0.042
Identities = 36/155 (23%), Positives = 60/155 (38%), Gaps = 19/155 (12%)
Query: 29 DVRREQWLSRVKKGCNVDSNGRMDNCPSSKHTASEENRSSCKEMTRKGEDIEGTCIQDSD 88
D+++ +W ++ N +S+G D + ++ R + ED + + D D
Sbjct: 152 DIKQWEWF--LEDSTNGNSDGDEDGDKKGRKGLGKKRRRK-----KGNEDDDWSVESDED 204
Query: 89 SRSTINS-----PDRSTYSHDESSGKDFSGSSSNSISTSCSGN-DSEEEDDDGCLDDWEA 142
+ S P ST S + S SSSN T G D EEEDDD +
Sbjct: 205 KELMVKSKRVVTPTYSTRSKKTKKDSNASSSSSNGAQTKQRGRADVEEEDDD------DE 258
Query: 143 VADALYANDKSHSVVSESPAEHEAECRYTVLEDDK 177
+D+ + E E E++ ED++
Sbjct: 259 TLGGFIVSDEEAKLEEEEEEEDESDVDDEEDEDEE 293
>At2g39100 RING zinc finger like protein
Length = 296
Score = 35.4 bits (80), Expect = 0.042
Identities = 17/51 (33%), Positives = 22/51 (42%)
Query: 245 QPSQCPICYEDLDVTDSSFLPCSCGFHLCLFCHKKILEADARCPSCRKLYD 295
Q CPIC E+L S+ + C CL C +K CP C +D
Sbjct: 35 QGKSCPICLENLTERRSAAVITVCKHGYCLACIRKWSSFKRNCPLCNTRFD 85
>At1g47900 mysoin-like protein
Length = 1054
Score = 35.4 bits (80), Expect = 0.042
Identities = 23/75 (30%), Positives = 38/75 (50%), Gaps = 6/75 (8%)
Query: 64 ENRSSCKEMTRKGEDIEGTCIQDSDSRSTINSPDRSTYSHDESS------GKDFSGSSSN 117
E+R+ C + T K + +E Q++ +S++ S+ SS G D SGS S
Sbjct: 432 ESRNLCAQSTSKLQSLEAQLQQNNSQKSSLEVCPNLNTSNPSSSISVSEDGNDDSGSCSG 491
Query: 118 SISTSCSGNDSEEED 132
S+ST+ S +E+D
Sbjct: 492 SLSTNPSQQIKKEKD 506
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.313 0.129 0.402
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,462,638
Number of Sequences: 26719
Number of extensions: 397426
Number of successful extensions: 1835
Number of sequences better than 10.0: 207
Number of HSP's better than 10.0 without gapping: 56
Number of HSP's successfully gapped in prelim test: 153
Number of HSP's that attempted gapping in prelim test: 1659
Number of HSP's gapped (non-prelim): 271
length of query: 322
length of database: 11,318,596
effective HSP length: 99
effective length of query: 223
effective length of database: 8,673,415
effective search space: 1934171545
effective search space used: 1934171545
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0081b.3