BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0079c.14
(171 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g15695 unknown protein 166 6e-42
At5g44250 unknown protein 155 1e-38
At3g07660 unknown protein 31 0.37
At5g64320 putative protein 30 0.64
At4g22020 glycine-rich protein 30 0.64
At1g18630 unknown protein 30 0.64
At4g15150 hypothetical protein 29 1.1
At2g46520 putative cellular apoptosis susceptibility protein 29 1.4
At1g74900 hypothetical protein 29 1.4
At1g65340 29 1.4
At2g18240 putative integral membrane protein 28 2.4
At3g58510 ATP-dependent RNA helicase-like protein 28 3.2
At3g24520 heat shock transcription factor HSF1, putative 28 3.2
At1g62240 unknown protein (At1g62240) 28 3.2
At1g75550 hypothetical protein 27 4.1
At3g58025 putative protein 27 5.4
At2g37590 putative DOF zinc finger protein 27 5.4
At1g70970 27 5.4
At1g63710 cytochrome P450 like protein 27 5.4
At1g05135 unknown protein 27 5.4
>At2g15695 unknown protein
Length = 408
Score = 166 bits (420), Expect = 6e-42
Identities = 80/165 (48%), Positives = 109/165 (65%), Gaps = 11/165 (6%)
Query: 17 VRGGNIYWGRKHATDFR-----------GIVVIFAWVSVPQTLLQDFVNLYSSLGWNSLV 65
+ GG +YWG+K + G+VVIF W S+ + L +FV+LYSSLGWNSLV
Sbjct: 2 IGGGRVYWGKKLDKEMEDAAVVDGGGSNGVVVIFVWSSINENQLMNFVDLYSSLGWNSLV 61
Query: 66 CYAHYLSAFRDESTVPLAFCVVDELIEELKTKSCPVVFAAFSAGSKACLYKVLQLIDGRC 125
C A +L+A E + LAF ++ EL+EELK++ CPV+F AFS KAC+YKVLQ+I C
Sbjct: 62 CRADFLTAVYPEMALSLAFHLLSELVEELKSRPCPVIFLAFSGAPKACMYKVLQVIMDDC 121
Query: 126 ETPHCLHNYHLLRNCVSGHIYDSGPLDVTSDFGFRFALHPSMAKV 170
E + L+R C+SGH+YDSGPLD TSD +FALHP++ ++
Sbjct: 122 EAQIHPDDSQLVRTCLSGHVYDSGPLDFTSDLNVKFALHPTIRRM 166
>At5g44250 unknown protein
Length = 403
Score = 155 bits (391), Expect = 1e-38
Identities = 76/154 (49%), Positives = 106/154 (68%), Gaps = 2/154 (1%)
Query: 19 GGNIYWGRKHAT--DFRGIVVIFAWVSVPQTLLQDFVNLYSSLGWNSLVCYAHYLSAFRD 76
GGN YW +K+ + IVV+FAW+S + L++ V+LYSSL W+SLVC++ +L+ F
Sbjct: 5 GGNYYWRKKNNNGGESEAIVVVFAWMSSEERNLKNHVDLYSSLLWDSLVCHSQFLNMFLP 64
Query: 77 ESTVPLAFCVVDELIEELKTKSCPVVFAAFSAGSKACLYKVLQLIDGRCETPHCLHNYHL 136
+ LA VV EL++ELK K P+VFA+FS G AC+YKVLQ+++G CET + L
Sbjct: 65 DKAADLASNVVSELVKELKAKPVPLVFASFSGGPNACMYKVLQILEGTCETGLNPDDCRL 124
Query: 137 LRNCVSGHIYDSGPLDVTSDFGFRFALHPSMAKV 170
+RNC+SG IYDS P+D TSD G R A+HP+ K+
Sbjct: 125 VRNCISGFIYDSCPVDFTSDLGARLAVHPTTLKM 158
>At3g07660 unknown protein
Length = 782
Score = 30.8 bits (68), Expect = 0.37
Identities = 22/78 (28%), Positives = 37/78 (47%), Gaps = 2/78 (2%)
Query: 4 AESEGGGGGGGCAVRGGNIYWGRKHAT-DFRGIVVIFAWVSVPQTLLQDFVNLYSSLGWN 62
AES+ GGGG RGG + + +HA+ D G F + P+ ++ + +
Sbjct: 19 AESQWRSGGGGRGSRGGRMNFSSRHASNDVAGAKNSFKKENGPKQVIDPSTSTSQEIKTK 78
Query: 63 SLVCYAHYLSAFRDESTV 80
+ + + SA D+STV
Sbjct: 79 DIALVSSH-SAVMDKSTV 95
>At5g64320 putative protein
Length = 730
Score = 30.0 bits (66), Expect = 0.64
Identities = 25/86 (29%), Positives = 36/86 (41%), Gaps = 21/86 (24%)
Query: 57 SSLGWNSLVCYAHYLSAFRDESTVPLAFCVVDELIEELKTKSCPVVFAAFSAGSKACLYK 116
+++G+N L+ SAF E +P A E+ E+ K C K +Y
Sbjct: 458 NTVGFNCLI------SAFCKEHRIPEAV----EIFREMPRKGC-----------KPDVYT 496
Query: 117 VLQLIDGRCETPHCLHNYHLLRNCVS 142
LI G CE H LLR+ +S
Sbjct: 497 FNSLISGLCEVDEIKHALWLLRDMIS 522
>At4g22020 glycine-rich protein
Length = 396
Score = 30.0 bits (66), Expect = 0.64
Identities = 14/32 (43%), Positives = 16/32 (49%)
Query: 3 GAESEGGGGGGGCAVRGGNIYWGRKHATDFRG 34
GA GGGGGGG G + G H + F G
Sbjct: 246 GAAGGGGGGGGGGGSGGSKVGGGYGHGSGFGG 277
Score = 28.5 bits (62), Expect = 1.9
Identities = 12/20 (60%), Positives = 14/20 (70%)
Query: 2 RGAESEGGGGGGGCAVRGGN 21
+G+ S GGGGGGG GGN
Sbjct: 318 KGSGSGGGGGGGGGGSGGGN 337
Score = 26.2 bits (56), Expect = 9.2
Identities = 11/19 (57%), Positives = 13/19 (67%)
Query: 2 RGAESEGGGGGGGCAVRGG 20
+GA+ GGGGGGG GG
Sbjct: 53 KGAKRYGGGGGGGGGGGGG 71
>At1g18630 unknown protein
Length = 155
Score = 30.0 bits (66), Expect = 0.64
Identities = 13/17 (76%), Positives = 14/17 (81%)
Query: 4 AESEGGGGGGGCAVRGG 20
A+S GGGGGGG A RGG
Sbjct: 112 ADSGGGGGGGGFARRGG 128
>At4g15150 hypothetical protein
Length = 102
Score = 29.3 bits (64), Expect = 1.1
Identities = 12/19 (63%), Positives = 13/19 (68%)
Query: 2 RGAESEGGGGGGGCAVRGG 20
RG + GGGGGGG A GG
Sbjct: 43 RGLKKPGGGGGGGGATSGG 61
>At2g46520 putative cellular apoptosis susceptibility protein
Length = 972
Score = 28.9 bits (63), Expect = 1.4
Identities = 28/92 (30%), Positives = 40/92 (43%), Gaps = 5/92 (5%)
Query: 25 GRKHATDFRGIVVIFAWVSVPQTLLQDFVNLYSSLGWNSLVCYAHYLSAFRDESTVPLAF 84
G +TD + FA + +P+ +D VN + L SL +L+ FR P A
Sbjct: 451 GASVSTDLIDVQNFFANIILPELQSRD-VNSFPMLKAGSL----KFLTMFRSHIPKPFAM 505
Query: 85 CVVDELIEELKTKSCPVVFAAFSAGSKACLYK 116
+ EL+ LK +S V A S K L K
Sbjct: 506 QLFPELVRFLKAESNVVHSYAASCIEKLLLVK 537
>At1g74900 hypothetical protein
Length = 482
Score = 28.9 bits (63), Expect = 1.4
Identities = 12/40 (30%), Positives = 20/40 (50%)
Query: 128 PHCLHNYHLLRNCVSGHIYDSGPLDVTSDFGFRFALHPSM 167
P L +H L N +++D+ D+ D R LHP++
Sbjct: 71 PKALQFFHFLDNHHREYVHDASSFDLAIDIAARLHLHPTV 110
>At1g65340
Length = 503
Score = 28.9 bits (63), Expect = 1.4
Identities = 15/42 (35%), Positives = 21/42 (49%), Gaps = 2/42 (4%)
Query: 103 FAAFSAGSKACLYKVLQLIDGRCETPHCLHNYHLLRNCVSGH 144
F AF+AG +ACL K L + + + NY + V GH
Sbjct: 441 FLAFNAGPRACLGKKLTFLQMKTVAAEIIRNYDI--KVVEGH 480
>At2g18240 putative integral membrane protein
Length = 566
Score = 28.1 bits (61), Expect = 2.4
Identities = 18/54 (33%), Positives = 26/54 (47%), Gaps = 8/54 (14%)
Query: 11 GGGGCAVRGGNIYWGRKHATDFRGIVVIFAWVSVPQTLLQDFVNLYSSLGWNSL 64
GGG A+ GGN K T VV+ W+ L+ +V Y+S G N++
Sbjct: 396 GGGNAAIFGGN---EGKETT-----VVLLGWLGAKAKHLRRYVEWYNSRGINAV 441
>At3g58510 ATP-dependent RNA helicase-like protein
Length = 612
Score = 27.7 bits (60), Expect = 3.2
Identities = 13/23 (56%), Positives = 16/23 (69%), Gaps = 3/23 (13%)
Query: 3 GAESEGGGGGGGCAVRGGNIYWG 25
G+ S GGGGGGG GG+ Y+G
Sbjct: 567 GSYSRGGGGGGG---GGGSDYYG 586
>At3g24520 heat shock transcription factor HSF1, putative
Length = 330
Score = 27.7 bits (60), Expect = 3.2
Identities = 15/33 (45%), Positives = 19/33 (57%), Gaps = 4/33 (12%)
Query: 5 ESEGGGGGGGC-AVRGGNIYWGRKHATDFRGIV 36
ES GGGGGGC +++G Y K F G+V
Sbjct: 282 ESVNGGGGGGCGSIQGERRY---KETATFGGVV 311
>At1g62240 unknown protein (At1g62240)
Length = 227
Score = 27.7 bits (60), Expect = 3.2
Identities = 11/17 (64%), Positives = 12/17 (69%)
Query: 4 AESEGGGGGGGCAVRGG 20
A GGGGGGG +V GG
Sbjct: 45 AAGGGGGGGGGASVEGG 61
>At1g75550 hypothetical protein
Length = 167
Score = 27.3 bits (59), Expect = 4.1
Identities = 12/23 (52%), Positives = 12/23 (52%)
Query: 3 GAESEGGGGGGGCAVRGGNIYWG 25
G GGGGGGG GG WG
Sbjct: 74 GGGGGGGGGGGGGGGGGGGWGWG 96
Score = 26.9 bits (58), Expect = 5.4
Identities = 12/23 (52%), Positives = 12/23 (52%)
Query: 3 GAESEGGGGGGGCAVRGGNIYWG 25
G GGGGGGG GG WG
Sbjct: 72 GGGGGGGGGGGGGGGGGGGGGWG 94
>At3g58025 putative protein
Length = 173
Score = 26.9 bits (58), Expect = 5.4
Identities = 13/29 (44%), Positives = 18/29 (61%), Gaps = 2/29 (6%)
Query: 3 GAESEGGGGG--GGCAVRGGNIYWGRKHA 29
G ++GGGGG GG RGG +Y +H+
Sbjct: 88 GLAAKGGGGGSRGGGRGRGGGVYAFGRHS 116
>At2g37590 putative DOF zinc finger protein
Length = 330
Score = 26.9 bits (58), Expect = 5.4
Identities = 10/17 (58%), Positives = 14/17 (81%)
Query: 6 SEGGGGGGGCAVRGGNI 22
S GGGGGGG ++R G++
Sbjct: 57 SGGGGGGGGGSIRAGSM 73
>At1g70970
Length = 402
Score = 26.9 bits (58), Expect = 5.4
Identities = 14/48 (29%), Positives = 26/48 (54%)
Query: 18 RGGNIYWGRKHATDFRGIVVIFAWVSVPQTLLQDFVNLYSSLGWNSLV 65
+GG +Y+G + +D R +V+ F S T ++ L+ S WN ++
Sbjct: 234 KGGVLYYGARSTSDHRPLVMSFNVRSQEFTAIELPDQLHISYFWNFVI 281
>At1g63710 cytochrome P450 like protein
Length = 523
Score = 26.9 bits (58), Expect = 5.4
Identities = 22/83 (26%), Positives = 35/83 (41%), Gaps = 4/83 (4%)
Query: 91 IEELKTKSC-PVVFAAFSAGSKACLYKVLQLIDGRCETPHCLHNYHLLRNCVSGH-IYDS 148
+EE + + C F AF+AG + CL K L + + T L + L GH +
Sbjct: 432 LEESRDEKCNQYKFVAFNAGPRICLGKDLAYLQMKSITASILLRHRL--TVAPGHRVEQK 489
Query: 149 GPLDVTSDFGFRFALHPSMAKVP 171
L + FG + +H +P
Sbjct: 490 MSLTLFMKFGLKMDVHKRDLTLP 512
>At1g05135 unknown protein
Length = 384
Score = 26.9 bits (58), Expect = 5.4
Identities = 12/25 (48%), Positives = 13/25 (52%)
Query: 8 GGGGGGGCAVRGGNIYWGRKHATDF 32
GGGGGGG GG G H + F
Sbjct: 209 GGGGGGGGGGGGGGANGGSGHGSGF 233
Score = 26.2 bits (56), Expect = 9.2
Identities = 11/18 (61%), Positives = 13/18 (72%)
Query: 8 GGGGGGGCAVRGGNIYWG 25
GGGGGGG V GG+ + G
Sbjct: 290 GGGGGGGGGVGGGSGHGG 307
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.325 0.141 0.461
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,278,796
Number of Sequences: 26719
Number of extensions: 189050
Number of successful extensions: 2427
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 29
Number of HSP's successfully gapped in prelim test: 15
Number of HSP's that attempted gapping in prelim test: 1913
Number of HSP's gapped (non-prelim): 314
length of query: 171
length of database: 11,318,596
effective HSP length: 92
effective length of query: 79
effective length of database: 8,860,448
effective search space: 699975392
effective search space used: 699975392
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0079c.14