BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0072a.1
(224 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g24490 putative replication protein A1 199 7e-52
At3g02920 replication factor A like protein 142 1e-34
At1g07131 unknown protein 46 1e-05
At5g23750 Unknown protein (MRO11.3) 32 0.21
At1g56010 NAC1 32 0.35
At2g47850 unknown protein 31 0.46
At5g21050 putative protein 29 1.7
At2g01650 unknown protein 29 2.3
At4g21270 kinesin-related protein katA 28 3.9
At3g22860 eukaryotic translation initiation factor 3 (subunit 8) 28 5.1
At4g37820 unknown protein 27 6.6
At2g42710 unknown protein 27 6.6
At5g50900 unknown protein 27 8.6
At3g28830 hypothetical protein 27 8.6
At3g09330 hypothetical protein 27 8.6
At2g01500 putative homeodomain transcription factor 27 8.6
>At2g24490 putative replication protein A1
Length = 279
Score = 199 bits (507), Expect = 7e-52
Identities = 106/230 (46%), Positives = 151/230 (65%), Gaps = 12/230 (5%)
Query: 1 MFSVTQFDSSSG--GGGFTSTQLN---DSSPAPQKGRESQGLVPVTVKQINEASQSGDEK 55
MFS +QF+ +SG GGGF S+Q + +SS + K R+ QGLVPVTVKQI E QS EK
Sbjct: 1 MFSSSQFEPNSGFSGGGFMSSQPSQAYESSSSTAKNRDFQGLVPVTVKQITECFQSSGEK 60
Query: 56 SSFVINGVELTNVTLVGMVFEKAERN-TDVNFVLDDGTGRIKCRRWINDAFDTQEVEEIM 114
S VING+ LTNV+LVG+V +K E T+V F LDDGTGRI C+RW+++ FD +E+E +
Sbjct: 61 SGLVINGISLTNVSLVGLVCDKDESKVTEVRFTLDDGTGRIDCKRWVSETFDAREMESVR 120
Query: 115 NGMYVRVNGHLKSFQGVRQVAAFSVRPVVNFDEIPFHFIDCIHHHLRSKLKMEGTISTDP 174
+G YVR++GHLK+FQG Q+ FSVRP+++F+E+ FH+I+CIH + ++ +
Sbjct: 121 DGTYVRLSGHLKTFQGKTQLLVFSVRPIMDFNEVTFHYIECIHFYSQNSESQRQQVGD-- 178
Query: 175 PASDSSLKTPVRNTSNGSQAPSSTPAYAQYGVD--GLKDCDKLVIDYLQQ 222
S+ T + SN +QA P + D G K+ D +++DYL+Q
Sbjct: 179 --VTQSVNTTFQGGSNTNQATLLNPVVSSQNNDGNGRKNLDDMILDYLKQ 226
>At3g02920 replication factor A like protein
Length = 278
Score = 142 bits (358), Expect = 1e-34
Identities = 84/224 (37%), Positives = 134/224 (59%), Gaps = 17/224 (7%)
Query: 8 DSSSGGGGF----TSTQLNDSSPAPQKGRESQGLVPVTVKQINEASQSGDEKSSFVINGV 63
+++ GGGF +TQ ++SS + K R+ + L+P+T+KQ++ AS +G+ S+F I+GV
Sbjct: 9 NAAFAGGGFMPSQATTQAHESSSS-LKNRDVRTLLPLTLKQLSSASTTGE--SNFSIDGV 65
Query: 64 ELTNVTLVGMVFEKAERNTDVNFVLDDGTGRIKCRRWINDAFDTQEVEEIMNGMYVRVNG 123
++ V +VG + R T V+FV+DDGTG + C RW + +T+E+E + GMYVR++G
Sbjct: 66 DIKTVVIVGRISRMENRITQVDFVVDDGTGWVDCVRWCHARQETEEMEAVKLGMYVRLHG 125
Query: 124 HLKSFQGVRQVAAFSVRPVVNFDEIPFHFIDCIHHHLRSKLKMEGTISTD-----PPASD 178
HLK FQG R V FSVRPV +F+EI HF +C++ H+ + G+I+ D P
Sbjct: 126 HLKIFQGKRSVNVFSVRPVTDFNEIVHHFTECMYVHMYNTKLRGGSITQDTATPRPQMPY 185
Query: 179 SSLKTPVRNTSNGSQAPSSTPAYAQYGVDGLKDCDKLVIDYLQQ 222
S++ TP + G PS+ + D + + V++YL Q
Sbjct: 186 STMPTPAKPYQTG---PSN--QFPNQFNDSMHGVKQTVLNYLNQ 224
>At1g07131 unknown protein
Length = 160
Score = 46.2 bits (108), Expect = 1e-05
Identities = 31/136 (22%), Positives = 60/136 (43%), Gaps = 19/136 (13%)
Query: 40 VTVKQINEASQSGDEKSSF-VINGVELTNVTLVGMVFEKAERNTDVNFVLDDGTGRIKCR 98
+ + I +QS E +SF ++ G ++ V +VG + + + F +DDGTG + C
Sbjct: 12 LVARDIQRLTQSPTESNSFSLLGGACVSRVEIVGTIVSRDLTPKFLKFGVDDGTGCVTCV 71
Query: 99 RWINDAFDT------------------QEVEEIMNGMYVRVNGHLKSFQGVRQVAAFSVR 140
W+N + ++ +I G RV G + S++GV Q+ A
Sbjct: 72 MWLNQLTSSYFSRWDPATILLLASAARKQAAQIRIGAVARVRGRVGSYRGVMQITANVAV 131
Query: 141 PVVNFDEIPFHFIDCI 156
+ + H+++C+
Sbjct: 132 AERDPNAEILHWLECL 147
>At5g23750 Unknown protein (MRO11.3)
Length = 202
Score = 32.3 bits (72), Expect = 0.21
Identities = 17/60 (28%), Positives = 30/60 (49%)
Query: 21 LNDSSPAPQKGRESQGLVPVTVKQINEASQSGDEKSSFVINGVELTNVTLVGMVFEKAER 80
L +PA +K +S+ +VPV K++ E + G V+ VE + +E+AE+
Sbjct: 46 LPSPAPAEEKQEDSKAIVPVVPKEVEEEKKEGSVNRDAVLARVETEKRMSLIKAWEEAEK 105
>At1g56010 NAC1
Length = 324
Score = 31.6 bits (70), Expect = 0.35
Identities = 23/88 (26%), Positives = 41/88 (46%), Gaps = 17/88 (19%)
Query: 141 PVVNFDEIPFHFIDCIHHHL-------RSKLKMEGTISTDPPASDSSLKTPVRNT----S 189
P +NFD+ P ++ HH++ S L T++++ S S LK P +N +
Sbjct: 200 PYINFDQEPSSYLSDDHHYIINEHVPCFSNLSQNQTLNSNLTNSVSELKIPCKNPNPLFT 259
Query: 190 NGSQAPSSTPAYAQYGVDGLKDCDKLVI 217
GS + + T G+D D++V+
Sbjct: 260 GGSASATLT------GLDSFCSSDQMVL 281
>At2g47850 unknown protein
Length = 553
Score = 31.2 bits (69), Expect = 0.46
Identities = 19/56 (33%), Positives = 27/56 (47%), Gaps = 1/56 (1%)
Query: 169 TISTDPPASDSSLKTPVRNTSNGSQAPSSTPAYAQYGVDGLKDCDKLVIDYLQQHS 224
T+ P D + P N S +QAP TPA A G+ GL C ++ ++L S
Sbjct: 61 TVHQIPKFKDMYARNPPLNGSQSAQAPDWTPADADTGLQGLA-CALILSNWLGSDS 115
>At5g21050 putative protein
Length = 355
Score = 29.3 bits (64), Expect = 1.7
Identities = 20/95 (21%), Positives = 42/95 (44%), Gaps = 5/95 (5%)
Query: 18 STQLNDSSPAPQKGRESQGLVPVTVKQINEASQSGDEKSSFVINGVELTNVTLVGMVFEK 77
S+ +DS P+P +S+ V + N Q+ + S ++ + + + ++ +
Sbjct: 4 SSSSHDSPPSPAITGDSETTVTNNESESNTKCQTAIQSLSTIVTNTNIPST--ITILLDD 61
Query: 78 AERNTDVNFVL---DDGTGRIKCRRWINDAFDTQE 109
+T ++ +L D G G RW+ D F + E
Sbjct: 62 EAVSTAISSLLLRPDSGAGDNNLCRWLYDTFQSAE 96
>At2g01650 unknown protein
Length = 458
Score = 28.9 bits (63), Expect = 2.3
Identities = 13/37 (35%), Positives = 20/37 (53%)
Query: 163 KLKMEGTISTDPPASDSSLKTPVRNTSNGSQAPSSTP 199
K K +G + +S + P++N N SQAP+ TP
Sbjct: 23 KFKGQGRVLGSSSSSSAPPVNPIQNRFNSSQAPNPTP 59
>At4g21270 kinesin-related protein katA
Length = 793
Score = 28.1 bits (61), Expect = 3.9
Identities = 28/95 (29%), Positives = 45/95 (46%), Gaps = 11/95 (11%)
Query: 2 FSVTQFDSSSGGGGFTSTQLNDSSPAPQKGRESQGLVPVTVKQINEASQS-GDE--KSSF 58
+ V F G G T T + QKG L+P +++QI +ASQS G + K
Sbjct: 508 YKVCIFAYGQTGSGKTYTMMGRPEAPDQKG-----LIPRSLEQIFQASQSLGAQGWKYKM 562
Query: 59 VINGVELTNVTLVGMVFEKAERNTDVNFV-LDDGT 92
++ +E+ N T+ ++ R T ++ V D GT
Sbjct: 563 QVSMLEIYNETIRDLL--STNRTTSMDLVRADSGT 595
>At3g22860 eukaryotic translation initiation factor 3 (subunit 8)
Length = 800
Score = 27.7 bits (60), Expect = 5.1
Identities = 21/88 (23%), Positives = 42/88 (46%), Gaps = 8/88 (9%)
Query: 43 KQINEASQ-SGDEKSSFVINGVELTNVTLVGMVFEKAER---NTDVNFVLDDGTGRIKCR 98
++I EA++ SG SSF++ + + F K+ R +T V+F+ +G R K R
Sbjct: 396 EEIEEANEESGSSSSSFIV----VAEIVPRKPTFAKSSRAMMDTLVSFIYKNGDERTKAR 451
Query: 99 RWINDAFDTQEVEEIMNGMYVRVNGHLK 126
+ D + ++ + + + HL+
Sbjct: 452 AMLCDIYQHALMDNFVTARDLLLMSHLQ 479
>At4g37820 unknown protein
Length = 532
Score = 27.3 bits (59), Expect = 6.6
Identities = 19/84 (22%), Positives = 41/84 (48%)
Query: 10 SSGGGGFTSTQLNDSSPAPQKGRESQGLVPVTVKQINEASQSGDEKSSFVINGVELTNVT 69
+ G G + +++ + + ES+G ++ ++ E + GD+ SS V++G E +
Sbjct: 125 AEGTGNEEGNEDSNNGESEKVVDESEGGNEISNEEAREINYKGDDASSEVMHGTEEKSNE 184
Query: 70 LVGMVFEKAERNTDVNFVLDDGTG 93
V + E +T+ V +D +G
Sbjct: 185 KVEVEGESKSNSTENVSVHEDESG 208
>At2g42710 unknown protein
Length = 415
Score = 27.3 bits (59), Expect = 6.6
Identities = 14/48 (29%), Positives = 27/48 (56%), Gaps = 2/48 (4%)
Query: 9 SSSGGGGFTSTQLNDSSPAPQKGRESQGLVPVTVKQINEASQSGDEKS 56
SSS +S+ +DS+P+P + VT++ ++ A++ D+KS
Sbjct: 32 SSSSSSSSSSSSSSDSNPSPDSNESRK--KAVTIEPVSYAAKPKDQKS 77
>At5g50900 unknown protein
Length = 555
Score = 26.9 bits (58), Expect = 8.6
Identities = 17/63 (26%), Positives = 32/63 (49%)
Query: 34 SQGLVPVTVKQINEASQSGDEKSSFVINGVELTNVTLVGMVFEKAERNTDVNFVLDDGTG 93
+QG+VPV V+ ++ S EK+ VI+ + + + ++ E + VL+ G+G
Sbjct: 184 AQGVVPVLVRLLDSCSLVMKEKTVAVISRISMVESSKHVLIAEGLSLLNHLLRVLESGSG 243
Query: 94 RIK 96
K
Sbjct: 244 FAK 246
>At3g28830 hypothetical protein
Length = 536
Score = 26.9 bits (58), Expect = 8.6
Identities = 18/53 (33%), Positives = 24/53 (44%), Gaps = 2/53 (3%)
Query: 5 TQFDSSSGGGGFTSTQLNDSSPAPQKGRESQGLVPVTVKQINEASQSGDEKSS 57
T+ SSGG T ++ + A K +ES G T K S SG K+S
Sbjct: 235 TKSKESSGGSAATKSKESSGGSAATKSKESSGGSATTGK--TSGSPSGSPKAS 285
>At3g09330 hypothetical protein
Length = 481
Score = 26.9 bits (58), Expect = 8.6
Identities = 16/54 (29%), Positives = 24/54 (43%), Gaps = 4/54 (7%)
Query: 8 DSSSGGGGFTSTQLNDSSPAPQKGRES----QGLVPVTVKQINEASQSGDEKSS 57
D ++S NDS+P P+ R+S G+ P T+ I+ S SS
Sbjct: 27 DEDDDSSSYSSLSQNDSAPWPRSYRQSVDILTGVTPPTISFIHRRSSQTSFTSS 80
>At2g01500 putative homeodomain transcription factor
Length = 271
Score = 26.9 bits (58), Expect = 8.6
Identities = 22/85 (25%), Positives = 35/85 (40%), Gaps = 7/85 (8%)
Query: 8 DSSSGGGGFTSTQLNDSSPAPQKGRESQGLVPVTVKQINEA-------SQSGDEKSSFVI 60
DSSSGG T+L S P + + L P + + N A ++ D+++S V
Sbjct: 138 DSSSGGHRVDQTKLCPSFPHTNRPQPQHELDPASYNKDNNANNEDHGTTEESDQRASEVG 197
Query: 61 NGVELTNVTLVGMVFEKAERNTDVN 85
N+ + + E N D N
Sbjct: 198 KYATWRNLVTWSITQQPEEINIDEN 222
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.315 0.132 0.380
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,144,534
Number of Sequences: 26719
Number of extensions: 216090
Number of successful extensions: 717
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 705
Number of HSP's gapped (non-prelim): 16
length of query: 224
length of database: 11,318,596
effective HSP length: 96
effective length of query: 128
effective length of database: 8,753,572
effective search space: 1120457216
effective search space used: 1120457216
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0072a.1