BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0066.12
(163 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g29440 putative protein 33 0.088
At5g46740 putative protein 31 0.34
At5g64410 Isp4-like protein 30 0.44
At1g67310 hypothetical protein 29 0.98
At3g19020 hypothetical protein 29 1.3
At4g03790 putative athila-like protein 28 1.7
At3g45780 nonphototropic hypocotyl 1 (NPH1) 28 1.7
At4g27520 early nodulin-like 2 predicted GPI-anchored protein 28 2.2
At1g05470 unknown protein 28 2.2
At5g55820 unknown protein 28 2.8
At4g32710 putative protein kinase 28 2.8
At1g51790 hypothetical protein 28 2.8
At5g62180 putative protein 27 3.7
At3g55420 unknown protein (At3g55420) 27 4.9
At1g17580 myosin 27 4.9
At4g33200 myosin - like protein 27 6.3
At3g51290 putative protein 27 6.3
At1g75710 unknown protein 27 6.3
At1g23420 INNER NO OUTER (INO) 27 6.3
At5g61140 RNA helicase - like protein 26 8.3
>At4g29440 putative protein
Length = 1071
Score = 32.7 bits (73), Expect = 0.088
Identities = 32/104 (30%), Positives = 43/104 (40%), Gaps = 14/104 (13%)
Query: 12 PNCSKPESSPSSTWFDMELPLPTT-------TSSETEPFRLDQAVCSHGLFMMAPNSWDP 64
P+ P+SS S DMELP + T S T P L V L P
Sbjct: 730 PSSIPPDSSSSDDESDMELPKRVSFRYQEKRTESRTRPTHLHSGVSHKDLEEEIPTR--- 786
Query: 65 LSNTLTRPLRLHDQDTDPSSSSPSFIVTVSQRSESIAVRVHHGT 108
++T ++ R H T P+S+S S+ T+S E VH T
Sbjct: 787 -ASTRSQDRRTH--KTTPASASASYFHTMSSDDED-EKEVHRDT 826
>At5g46740 putative protein
Length = 732
Score = 30.8 bits (68), Expect = 0.34
Identities = 33/98 (33%), Positives = 41/98 (41%), Gaps = 17/98 (17%)
Query: 1 MEELEKPPPCN---------PNCSKPESSPSSTWFDMELPLPTTTSSETEPFRLDQAVCS 51
M E PPP N PN + ESSP++ D L T + S + P R QA+
Sbjct: 1 MAEFSDPPPSNLSSSHKLTKPNQTLDESSPTAPIRD----LVTNSLSLSSPIRQIQALSP 56
Query: 52 HGLFMMAPNSWDPLSNTLT-RPLRLHDQDTDPSSSSPS 88
+S P TL P D + D SSSSPS
Sbjct: 57 ---AKPDGSSSSPPDKTLNFNPEENRDVNPDESSSSPS 91
>At5g64410 Isp4-like protein
Length = 729
Score = 30.4 bits (67), Expect = 0.44
Identities = 9/28 (32%), Positives = 19/28 (67%)
Query: 120 MVPTSLTASLNSWVFIGKLISVVLFRFR 147
M+P + + NSW+ +G + ++ +FR+R
Sbjct: 636 MMPPATAVNYNSWILVGTIFNLFVFRYR 663
>At1g67310 hypothetical protein
Length = 1016
Score = 29.3 bits (64), Expect = 0.98
Identities = 12/23 (52%), Positives = 15/23 (65%)
Query: 2 EELEKPPPCNPNCSKPESSPSST 24
E EKP C P CS+P++S ST
Sbjct: 529 EYREKPDTCCPKCSEPQTSDMST 551
>At3g19020 hypothetical protein
Length = 951
Score = 28.9 bits (63), Expect = 1.3
Identities = 26/99 (26%), Positives = 37/99 (37%), Gaps = 1/99 (1%)
Query: 2 EELEKPPPCNPNCSKPESSPSSTWFDMELPLPTTTSSETEPFRLDQAVCSHGLFMMAPNS 61
EE KP P KPE SP E P P S + +P + +Q + M +P
Sbjct: 542 EESPKPQPPKQETPKPEESPKPQPPKQETPKP-EESPKPQPPKQEQPPKTEAPKMGSPPL 600
Query: 62 WDPLSNTLTRPLRLHDQDTDPSSSSPSFIVTVSQRSESI 100
P+ N + + P S S T S +S +
Sbjct: 601 ESPVPNDPYDASPIKKRRPQPPSPSTEETKTTSPQSPPV 639
>At4g03790 putative athila-like protein
Length = 1064
Score = 28.5 bits (62), Expect = 1.7
Identities = 37/145 (25%), Positives = 57/145 (38%), Gaps = 21/145 (14%)
Query: 4 LEKPPPCNPNCSKPESSPSSTWFDMELPLPTTTSSETEPFRLDQAVCSH--GLFMMAPNS 61
+E P P + N S P P ST P P+ T LD + S L+ + P+S
Sbjct: 599 IENPTPSSHNHSTPSPYPISTHHSTSTPSPSLTY-----HLLDLVISSTPIKLYPIPPHS 653
Query: 62 WDPLSN-TLTRPLRLHDQDTDPSSSSPSFIVTVSQRSESIAVRVHHGTHLLSPHEVRALM 120
P + + ++PL L S+S+ + S S +VR H H + A
Sbjct: 654 SSPTNQLSYSQPLDLLTIFFIYSTST-----SFSTHQTSSSVRFH--------HSIAAAF 700
Query: 121 VPTSLTASLNSWVFIGKLISVVLFR 145
V ++ W IG + + L R
Sbjct: 701 VSLPPPPLIHLWATIGNNLPLNLAR 725
>At3g45780 nonphototropic hypocotyl 1 (NPH1)
Length = 996
Score = 28.5 bits (62), Expect = 1.7
Identities = 12/36 (33%), Positives = 21/36 (58%)
Query: 5 EKPPPCNPNCSKPESSPSSTWFDMELPLPTTTSSET 40
++P P NP S E + +++W ++ P P T S +T
Sbjct: 65 QEPAPSNPVRSDQEIAVTTSWMALKDPSPETISKKT 100
>At4g27520 early nodulin-like 2 predicted GPI-anchored protein
Length = 349
Score = 28.1 bits (61), Expect = 2.2
Identities = 24/81 (29%), Positives = 32/81 (38%), Gaps = 3/81 (3%)
Query: 8 PPCNPNCSKPESSPSSTWFDMELPLPTTTSSETEPFRLDQAVCSHGLFMMAPNSWDPLSN 67
PP P + P S S+ P+ +SS P A + MAP S P+SN
Sbjct: 217 PPAPPKSTSPVSPSSAPMTSPPAPMAPKSSSTIPP---SSAPMTSPPGSMAPKSSSPVSN 273
Query: 68 TLTRPLRLHDQDTDPSSSSPS 88
+ T L + SS S S
Sbjct: 274 SPTVSPSLAPGGSTSSSPSDS 294
>At1g05470 unknown protein
Length = 585
Score = 28.1 bits (61), Expect = 2.2
Identities = 21/69 (30%), Positives = 28/69 (40%), Gaps = 12/69 (17%)
Query: 20 SPSSTWFDMELPLPTTTSSETEPFRLDQ--AVCSHGLFMMAPNSWDPLSNTLTRPLRLHD 77
+PSSTW D +P P LD+ +VC F P+ +DP + R D
Sbjct: 176 TPSSTWNDPSIPQP----------GLDRRFSVCDRVFFSHRPSDFDPSFRGSSSSHRPSD 225
Query: 78 QDTDPSSSS 86
PS S
Sbjct: 226 YSRRPSDYS 234
>At5g55820 unknown protein
Length = 1826
Score = 27.7 bits (60), Expect = 2.8
Identities = 16/64 (25%), Positives = 29/64 (45%)
Query: 65 LSNTLTRPLRLHDQDTDPSSSSPSFIVTVSQRSESIAVRVHHGTHLLSPHEVRALMVPTS 124
LS+ L R + H + D + +SP + + ES+++ SP + V T+
Sbjct: 169 LSHNLVRQVSNHSHEQDVNIASPRDVHEKERLPESVSIDCRENQSCSSPEHSKNQRVETN 228
Query: 125 LTAS 128
L A+
Sbjct: 229 LDAT 232
>At4g32710 putative protein kinase
Length = 731
Score = 27.7 bits (60), Expect = 2.8
Identities = 15/40 (37%), Positives = 21/40 (52%), Gaps = 1/40 (2%)
Query: 4 LEKPPPCNPNCSKPESSPSSTWFDME-LPLPTTTSSETEP 42
LE P P +P+ S P SP + + P P++ SET P
Sbjct: 92 LESPSPPSPHVSAPSGSPPLPFLPAKPSPPPSSPPSETVP 131
>At1g51790 hypothetical protein
Length = 838
Score = 27.7 bits (60), Expect = 2.8
Identities = 17/46 (36%), Positives = 22/46 (46%), Gaps = 10/46 (21%)
Query: 9 PCNPNCSKPESSPSSTWFDMELPLPTTTSSETEPFRLDQAVCSHGL 54
P PN +KP S PS+ + TS+ T FRL Q V G+
Sbjct: 219 PATPNGTKPLSDPST----------SLTSNSTGNFRLPQVVMRTGI 254
>At5g62180 putative protein
Length = 327
Score = 27.3 bits (59), Expect = 3.7
Identities = 16/49 (32%), Positives = 19/49 (38%), Gaps = 2/49 (4%)
Query: 7 PPPCNPNCSK--PESSPSSTWFDMELPLPTTTSSETEPFRLDQAVCSHG 53
P P NP SK P + STW + LP +L V HG
Sbjct: 40 PSPLNPAVSKDLPVNQLKSTWLRLYLPSSAVNEGNVSSQKLPIVVYYHG 88
>At3g55420 unknown protein (At3g55420)
Length = 216
Score = 26.9 bits (58), Expect = 4.9
Identities = 11/31 (35%), Positives = 16/31 (51%)
Query: 82 PSSSSPSFIVTVSQRSESIAVRVHHGTHLLS 112
PSSSSP + T + + + HH H L+
Sbjct: 61 PSSSSPPLLSTAAYLPTNFTILAHHAWHTLT 91
>At1g17580 myosin
Length = 1520
Score = 26.9 bits (58), Expect = 4.9
Identities = 16/41 (39%), Positives = 22/41 (53%), Gaps = 2/41 (4%)
Query: 116 VRALMVPTSLTASLNSWVFIGKLISVVLFRFRLRCRECCDF 156
+RA VP+ L + + +F I+V LF L RECC F
Sbjct: 1326 MRANYVPSLLISKVFGQIF--SFINVQLFNSLLLRRECCSF 1364
>At4g33200 myosin - like protein
Length = 1374
Score = 26.6 bits (57), Expect = 6.3
Identities = 24/91 (26%), Positives = 36/91 (39%), Gaps = 8/91 (8%)
Query: 72 PLRLHDQDTDPSSSSPSFIVTVSQRSESIAVRVHHGT------HLLSPHEVRALMVPTSL 125
P +LH D S + + ++ + V +G LSP + VP+
Sbjct: 1159 PFKLHGPDDGASHIEARYPALLFKQQLTACVEKIYGLIRDNLKKELSPLLGSCIQVPSFF 1218
Query: 126 TASLNSWVFIGKLISVVLFRFRLRCRECCDF 156
L + VF I++ LF L RECC F
Sbjct: 1219 IRKLVTQVF--SFINLSLFNSLLLRRECCTF 1247
>At3g51290 putative protein
Length = 602
Score = 26.6 bits (57), Expect = 6.3
Identities = 24/94 (25%), Positives = 37/94 (38%), Gaps = 10/94 (10%)
Query: 7 PPPCNPNCSKPESSPSSTW--FDMELPLPTTTSSE------TEPFRLDQAVCSHGLFMMA 58
PPP P P PSSTW +D +P P ++S E T R S A
Sbjct: 105 PPPPPP--PPPPPPPSSTWDFWDPFIPPPPSSSEEEWEEETTTATRTATGTGSDAAVTTA 162
Query: 59 PNSWDPLSNTLTRPLRLHDQDTDPSSSSPSFIVT 92
P + P ++++ T + S + +V+
Sbjct: 163 PTTATPQASSVVSGFSKDTMTTTTTGSELAVVVS 196
>At1g75710 unknown protein
Length = 462
Score = 26.6 bits (57), Expect = 6.3
Identities = 26/97 (26%), Positives = 38/97 (38%), Gaps = 18/97 (18%)
Query: 2 EELEKPPPCNPNCSKPESSPSSTWFDMELPLPTTTSSETEPFRLDQAVCSHGLFMMAPNS 61
E E P P SK + + P T + + +P + +AV P+S
Sbjct: 9 ENAEAPQKHKPTSSKRKKRDN--------PTDQTQTQKHKPQKPKKAVPPK-----QPSS 55
Query: 62 WDPLSNTLT----RPLRLHDQDTDPSSSSPSFIVTVS 94
WD + N LT R+HD + S S PS +S
Sbjct: 56 WDQIKNLLTCKQIEGSRVHDPSKN-SQSGPSMTTNLS 91
>At1g23420 INNER NO OUTER (INO)
Length = 231
Score = 26.6 bits (57), Expect = 6.3
Identities = 17/47 (36%), Positives = 24/47 (50%)
Query: 91 VTVSQRSESIAVRVHHGTHLLSPHEVRALMVPTSLTASLNSWVFIGK 137
V + S + VR H T LLS + ++A +P L ASL+ GK
Sbjct: 37 VPFTSLSMVVTVRCGHCTSLLSVNLMKASFIPLHLLASLSHLDETGK 83
>At5g61140 RNA helicase - like protein
Length = 2139
Score = 26.2 bits (56), Expect = 8.3
Identities = 13/23 (56%), Positives = 15/23 (64%)
Query: 26 FDMELPLPTTTSSETEPFRLDQA 48
F M + LP TSS EPF +DQA
Sbjct: 70 FVMLVQLPRLTSSLREPFDIDQA 92
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.319 0.132 0.414
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,158,251
Number of Sequences: 26719
Number of extensions: 166457
Number of successful extensions: 600
Number of sequences better than 10.0: 27
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 18
Number of HSP's that attempted gapping in prelim test: 586
Number of HSP's gapped (non-prelim): 29
length of query: 163
length of database: 11,318,596
effective HSP length: 92
effective length of query: 71
effective length of database: 8,860,448
effective search space: 629091808
effective search space used: 629091808
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0066.12