BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0065.6
(516 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g03820 putative nonsense-mediated mRNA decay protein 712 0.0
At1g56660 hypothetical protein 34 0.17
At3g28770 hypothetical protein 33 0.29
At1g79280 hypothetical protein 32 0.84
At5g54410 unknown protein 32 1.1
At3g11330 unknown protein 32 1.1
At2g45540 unknown protein 32 1.1
At1g80810 unknown protein 32 1.1
At5g63550 unknown protein 31 1.4
At1g44900 transcription factor, putative 31 1.4
At1g19100 hypothetical protein 31 1.4
At3g01770 unknown protein 31 1.9
At1g67020 hypothetical protein 31 1.9
At2g30500 unknown protein 30 3.2
At5g63320 putative protein 30 4.2
At3g45590 putative protein 30 4.2
At3g04380 Su(VAR)3-9-related protein 4 30 4.2
At5g43560 unknown protein 29 5.5
At1g11510 hypthetical protein 29 5.5
At4g35560 hypothetical protein 29 7.1
>At2g03820 putative nonsense-mediated mRNA decay protein
Length = 516
Score = 712 bits (1838), Expect = 0.0
Identities = 345/515 (66%), Positives = 424/515 (81%), Gaps = 12/515 (2%)
Query: 6 EEAGMFTVHRTVGSVLCCKCGIPMQPNAANMCVKCLRSEVDITEGLLKRLVLVHCPECES 65
+E+GMF V +T+GSVLCCKCG+PM PNAANMCV CLRSEVDITEGL K + + +CPEC
Sbjct: 5 DESGMFNVQQTIGSVLCCKCGVPMAPNAANMCVNCLRSEVDITEGLQKSIQIFYCPECTC 64
Query: 66 YLQPPRTWIKLQLESKELLTFCLKKLQKNMSSSKVRMVHAEFIWTEPHSKRIKVNVKVQK 125
YLQPP+TWIK Q ESKELLTFC+K+L KN++ KV++ +AEF+WTEPHSKRIKV + VQ
Sbjct: 65 YLQPPKTWIKCQWESKELLTFCIKRL-KNLN--KVKLKNAEFVWTEPHSKRIKVKLTVQA 121
Query: 126 EVLNGAILEQSYLVEYVQQEHLCESCSKVAANPDQWVAAVQLRQHVSHRRTFFYLEQLIL 185
EVLNGA+LEQSY VEY +++LCESCS+ ANPDQWVA++QLRQHVSHRRTFFYLEQLIL
Sbjct: 122 EVLNGAVLEQSYPVEYTVRDNLCESCSRFQANPDQWVASIQLRQHVSHRRTFFYLEQLIL 181
Query: 186 KHGAAASAIRIKQMDEGIDFYFSNRSHGVKFVEFIGKVAPIKSRSDKQLVSHDTKSNEYN 245
+H AA+ AIRI+Q+D+GIDF+F N+SH FVEF+ KV PI+ R D+QLVSHD KS+ YN
Sbjct: 182 RHDAASRAIRIQQVDQGIDFFFGNKSHANSFVEFLRKVVPIEYRQDQQLVSHDVKSSLYN 241
Query: 246 YKHTFSVEISPICREDLICLPPKVALSLGNLGPIVICTKVTNSIALLDPFTLRYCFLDAD 305
YK+T+SV+I P+CREDL+CLP KVA LGNLGP+V+CTKV+++I LLDP TLR FLDA
Sbjct: 242 YKYTYSVKICPVCREDLVCLPSKVASGLGNLGPLVVCTKVSDNITLLDPRTLRCAFLDAR 301
Query: 306 QYWRSSFKSLLTSRQLVEYIVLDVEVVSSEVTVGGTKYVLADAQVARVSDFGKNDTIFSI 365
QYWRS F+S LTSRQLV+Y V DVE E TVGG KY L+ Q+AR SD GK +F +
Sbjct: 302 QYWRSGFRSALTSRQLVKYFVFDVEPPVGEATVGGQKYALSYVQIARESDIGK---MFYV 358
Query: 366 KTHLGHLLNPGDYALGYDLYGANNNDIELDKYKGLI---LPDAILVKKSYEEKRQKKRGK 422
+THLGH+L PGD ALGYD+YGAN ND E++KY+ + LP+AIL+KK YEE+R++K+ K
Sbjct: 359 QTHLGHILKPGDQALGYDIYGANVNDNEMEKYRLSVKNGLPEAILIKKCYEEQRERKQKK 418
Query: 423 ARSWKLKSLNMEVDDKVARADPDKMHSEYEQFLKDLEENPDLRFNISLYRNKEYQPSEIA 482
+R+WKLKSL ME+DD R DP+K EYE+FL+DLEENP+LRFNISLYR+K+YQ SE A
Sbjct: 419 SRNWKLKSLPMEMDDSRGRVDPEKTDKEYEEFLRDLEENPELRFNISLYRDKDYQASETA 478
Query: 483 SVTDGEELPSVPLEELLADLELG---EDEDEEDDM 514
S+TDGE PSVP+EELLADL+L ED+D+EDDM
Sbjct: 479 SMTDGEGAPSVPIEELLADLDLSFEEEDDDDEDDM 513
>At1g56660 hypothetical protein
Length = 522
Score = 34.3 bits (77), Expect = 0.17
Identities = 26/103 (25%), Positives = 47/103 (45%), Gaps = 11/103 (10%)
Query: 409 KKSYEEKRQKKRGKARSWKLKSLNMEVDDKVARADPDKMHSEYEQFLKDLEENPDLRFNI 468
+K +EE ++K GK + K + ++K +AD +K H + Q ++LEE
Sbjct: 120 EKKHEELEEEKEGKKKKNKKEKDESGPEEKNKKADKEKKHEDVSQEKEELEEEDG----- 174
Query: 469 SLYRNKEYQPSEIASVTDGEELPSVPLEELLADLELGEDEDEE 511
+NK+ + E + EE P +E E +ED++
Sbjct: 175 --KKNKKKEKDESGT----EEKKKKPKKEKKQKEESKSNEDKK 211
>At3g28770 hypothetical protein
Length = 2081
Score = 33.5 bits (75), Expect = 0.29
Identities = 23/72 (31%), Positives = 36/72 (49%), Gaps = 9/72 (12%)
Query: 409 KKSYEEKRQKKRGKARSWKLKSLNMEVDDKVARADPDKMHSEYEQFLKDLEENPDLRFNI 468
KK YEEK+ K + +A+ K KS + + ++K SE + K+ EE+ DL+
Sbjct: 1012 KKEYEEKKSKTKEEAKKEKKKSQDKKREEK---------DSEERKSKKEKEESRDLKAKK 1062
Query: 469 SLYRNKEYQPSE 480
KE + SE
Sbjct: 1063 KEEETKEKKESE 1074
Score = 28.5 bits (62), Expect = 9.3
Identities = 25/112 (22%), Positives = 44/112 (38%), Gaps = 4/112 (3%)
Query: 409 KKSYEEKRQKKRGKARSWKLKSLNMEVDDKVARADPDKMHSEYEQFLKDLEENPDLRFNI 468
K+S K +KK K KS+ E D K + + + E+ KD+E+ D N
Sbjct: 1071 KESENHKSKKKEDKKEHEDNKSMKKEEDKKEKKKHEESKSRKKEEDKKDMEKLEDQNSNK 1130
Query: 469 SLYRNKEYQPSE----IASVTDGEELPSVPLEELLADLELGEDEDEEDDMME 516
E + S+ + +D +E + ++E + + E D E
Sbjct: 1131 KKEDKNEKKKSQHVKLVKKESDKKEKKENEEKSETKEIESSKSQKNEVDKKE 1182
>At1g79280 hypothetical protein
Length = 2111
Score = 32.0 bits (71), Expect = 0.84
Identities = 20/81 (24%), Positives = 36/81 (43%), Gaps = 1/81 (1%)
Query: 434 EVDDKVARADPDKMHSEYE-QFLKDLEENPDLRFNISLYRNKEYQPSEIASVTDGEELPS 492
E + + + DK E + L+DLEE D+ + +E QP +A+ T
Sbjct: 1892 ENQEPLTDMESDKEEGELDLDTLEDLEEGTDVASMMRSPEKEEVQPETLATPTQSPSRME 1951
Query: 493 VPLEELLADLELGEDEDEEDD 513
+EE +E ++D+ D+
Sbjct: 1952 TAMEEAETTIETPVEDDKTDE 1972
>At5g54410 unknown protein
Length = 219
Score = 31.6 bits (70), Expect = 1.1
Identities = 29/108 (26%), Positives = 48/108 (43%), Gaps = 16/108 (14%)
Query: 409 KKSYEEKRQKKRGKARSWKLKSLNMEVDDKVARADPDKMHSEYEQFLKDLEENPDLRFNI 468
K+ EKR++ A+ K + +V++K+ + P+K +E E+ KDL E
Sbjct: 68 KRLENEKRKQALKDAKDLKDLTYKTKVENKLKKTQPEKDRAEEEE--KDLTE-------- 117
Query: 469 SLYRNKEYQPSEIASVTDGEELPSVPLEELLAD--LELGEDEDEEDDM 514
K+ P+E EE P EE D E +D EE+++
Sbjct: 118 ----EKKKDPTEEEEKDPTEEKKKEPAEEKKKDPTEEKKKDPAEEEEL 161
>At3g11330 unknown protein
Length = 499
Score = 31.6 bits (70), Expect = 1.1
Identities = 23/99 (23%), Positives = 47/99 (47%), Gaps = 16/99 (16%)
Query: 395 DKYKGLILPDAILVKKSYEEKRQKKRGKARSWKLKSLNMEVDDKVARADPDKMHSEYEQF 454
+ ++ + L DA + +EKR+++ + ++W L + D++H+ YE+
Sbjct: 109 ESFEDIALTDAA----AKDEKRRQEMDQEKTWCESILKL-----------DEVHASYEKL 153
Query: 455 LKDLEENPDLRFNISLYRNKEYQPSEIASVTDGEELPSV 493
LK+ EE +R S +N +A+V EE+ +
Sbjct: 154 LKEAEERL-VRIYESAEKNAAEDEENVAAVEVNEEVVGI 191
>At2g45540 unknown protein
Length = 2946
Score = 31.6 bits (70), Expect = 1.1
Identities = 13/39 (33%), Positives = 23/39 (58%)
Query: 374 NPGDYALGYDLYGANNNDIELDKYKGLILPDAILVKKSY 412
NP D +G+DL G + N +E D L +P+++ +K +
Sbjct: 996 NPHDNEIGFDLPGPDGNSVEDDNVGSLNVPESVRQEKEH 1034
>At1g80810 unknown protein
Length = 826
Score = 31.6 bits (70), Expect = 1.1
Identities = 39/140 (27%), Positives = 54/140 (37%), Gaps = 15/140 (10%)
Query: 392 IELDKYKGLILPDAILVKKSYEEKRQKKRGKARSWK----LKSLNMEVDDKV-------- 439
+E G+ L+KK ++ K+ K R K LK LN E D
Sbjct: 649 VEPTSSSGVRSSSRTLMKKDCGKRLNKQVEKTREGKNLRSLKELNAETDRTAEEQEVSLE 708
Query: 440 ARADPDKMHSEYEQFLKDL-EENPDLRFNISLYRN-KEYQPSEIASVTDGEELPSVPLEE 497
A +D EYE D E++ D K+Y SE S + E P
Sbjct: 709 AESDDRSEEQEYEDDCSDKKEQSQDKGVEAETKEEEKQYPNSEGESEGEDSESEEEPKWR 768
Query: 498 LLADLELGEDEDEED-DMME 516
D+E E+E+EE+ D ME
Sbjct: 769 ETDDMEDDEEEEEEEIDHME 788
>At5g63550 unknown protein
Length = 530
Score = 31.2 bits (69), Expect = 1.4
Identities = 28/121 (23%), Positives = 54/121 (44%), Gaps = 7/121 (5%)
Query: 392 IELDKYKGLILPDAILVKKSYEEKRQKKRGKARSWKLKSLNMEVDDKVARADPDKMHSEY 451
+E K K P K S E+ + K+GKA++ + +EV K+ + + D +
Sbjct: 392 VESSKEKSKKQPSKPQAKGSKEKGKATKKGKAKAEPTRKEMLEVVSKILK-EVDFNTATL 450
Query: 452 EQFLKDLEENPDLRFNISLYRNKEYQPSEIASVTDGEELPSVPLEELLADLELGEDEDEE 511
L+ L ++ + + K+ I ++TD EE E+ + E G D+++E
Sbjct: 451 SDILQKLSDHFGVELSHRKPEVKDVITEAINAMTDDEE------EDEEEEAEAGSDKEKE 504
Query: 512 D 512
+
Sbjct: 505 E 505
>At1g44900 transcription factor, putative
Length = 928
Score = 31.2 bits (69), Expect = 1.4
Identities = 22/76 (28%), Positives = 37/76 (47%), Gaps = 1/76 (1%)
Query: 435 VDDKVARADPDKMHSEYEQFLKDLEENPDLRFNISLYRNKEYQPSEI-ASVTDGEELPSV 493
VD +V R +PD+ E E+ +DL + + + N +Y+ + I SV D +L
Sbjct: 45 VDTQVIRDEPDEAEDEEEEEGEDLFNDTFMNDYRKMDENDQYESNGIDDSVDDERDLGQA 104
Query: 494 PLEELLADLELGEDED 509
L+ AD +L E+
Sbjct: 105 MLDRRAADADLDAREN 120
>At1g19100 hypothetical protein
Length = 542
Score = 31.2 bits (69), Expect = 1.4
Identities = 15/46 (32%), Positives = 27/46 (58%)
Query: 414 EKRQKKRGKARSWKLKSLNMEVDDKVARADPDKMHSEYEQFLKDLE 459
E+ +K R K K++S N+EV R++ + SEYE+ + +L+
Sbjct: 482 EENKKLRAKCLDRKVRSQNLEVKAMNLRSELENYKSEYERLMVELQ 527
>At3g01770 unknown protein
Length = 620
Score = 30.8 bits (68), Expect = 1.9
Identities = 38/131 (29%), Positives = 62/131 (47%), Gaps = 22/131 (16%)
Query: 391 DIELDKYKGLILPDAILVKKSYEEKRQKKRGKARSWKLKSLNMEVDDKVARADPDKMHSE 450
++EL K K A L ++ E + +++ +A+ K K +E++ + AR +M
Sbjct: 486 ELELQKKK----EKARLQAEAKEAEEARRKAEAQEAKRK---LELEREAARQALLEMEKS 538
Query: 451 YE-----QFLKDLEENPDLRFNISLYRNKEYQPSEIASVTDGEEL----PSVPLEELLAD 501
E +FLKDLE + N RN ++ S +DG + S PLE+L
Sbjct: 539 VEINENTRFLKDLELLKTV--NTDQLRNLR----DVGSESDGLAVFGFGGSNPLEQLGLF 592
Query: 502 LELGEDEDEED 512
++ EDEDE D
Sbjct: 593 MKHEEDEDESD 603
>At1g67020 hypothetical protein
Length = 659
Score = 30.8 bits (68), Expect = 1.9
Identities = 26/130 (20%), Positives = 57/130 (43%), Gaps = 14/130 (10%)
Query: 387 ANNNDIELDKYKGLILPDAILVKKSYEEKRQKKRGKARSWKLKSLNMEVDDKVARADP-- 444
ANN+ K+ + D IL++ ++R+K ++W K N + ++ DP
Sbjct: 268 ANNSSDSKLKFGAYKVLDKILIRSHNLKQRRKLSLSPKNWMFKFKNRNLQQNRSKMDPCQ 327
Query: 445 ----DKMHSEYEQF------LKDLEENPDLRFNISLYRNKEYQPSEIASVTDGEELPSVP 494
+H Y F ++ + LR + +++ + ++ S++ E P++P
Sbjct: 328 AIEFSNIHYMYLCFSANVGLVRKNKSFQSLRLKKQAHMSRDLK--KLESISSEELQPALP 385
Query: 495 LEELLADLEL 504
+ E L D ++
Sbjct: 386 IVETLGDEDM 395
>At2g30500 unknown protein
Length = 517
Score = 30.0 bits (66), Expect = 3.2
Identities = 28/96 (29%), Positives = 46/96 (47%), Gaps = 24/96 (25%)
Query: 383 DLYGANNNDIELDKY---------KGLILPDAILV---------KKSYEEKRQKKRGKAR 424
D GA ++LDK K L L + + + K++Y K + + GKA
Sbjct: 237 DHLGAEQKSVDLDKEDTKEDAVATKVLALEEELSIAKEKLQHFEKETYSLKNELEIGKAA 296
Query: 425 SWKLKSLNMEVDDKVARADPD----KMHSEYEQFLK 456
KLKSL E++ +A+ D D K+++E ++ LK
Sbjct: 297 EEKLKSLQHELE--LAQRDADTYINKLNAEKKEVLK 330
>At5g63320 putative protein
Length = 569
Score = 29.6 bits (65), Expect = 4.2
Identities = 29/126 (23%), Positives = 52/126 (41%), Gaps = 29/126 (23%)
Query: 413 EEKRQKKRGKARSWKLKSLNMEVDDKVARADPDKMHSEYEQFLKDLEENPDLRFNISLYR 472
E++R + KA + E +K AR + ++ Q L+ +E+ ++ I
Sbjct: 113 EKERLQAEAKAAEEARRKAKAEAAEK-ARREREQEREAARQALQKMEKTVEINEGIRFME 171
Query: 473 NKEYQPSEIASVTDGEELP-------------------------SVPLEELLADLELGED 507
+ + A+ T+G++LP S PLE L +++ ED
Sbjct: 172 DLQMLR---ATGTEGDQLPTSMEVMSPKFSEDMLGLGSFKMESNSNPLEHLGLYMKMDED 228
Query: 508 EDEEDD 513
EDEE+D
Sbjct: 229 EDEEED 234
>At3g45590 putative protein
Length = 237
Score = 29.6 bits (65), Expect = 4.2
Identities = 21/77 (27%), Positives = 36/77 (46%), Gaps = 7/77 (9%)
Query: 390 NDIELDKYKGLILPDAILVKKSYEEKRQKKRGKARSWKLKS-LNMEVDDKVARADPDKMH 448
N+++L + D ++ K+Y R K +W ++S L VD V R P +H
Sbjct: 103 NEVDLWRSMSSFKQDFAILYKAYSHLRSK------NWIVRSGLQYGVDFVVYRHHPSLVH 156
Query: 449 SEYEQFLKDLEENPDLR 465
SEY ++ + N L+
Sbjct: 157 SEYAVLVQSIGGNDRLK 173
>At3g04380 Su(VAR)3-9-related protein 4
Length = 492
Score = 29.6 bits (65), Expect = 4.2
Identities = 27/110 (24%), Positives = 51/110 (45%), Gaps = 9/110 (8%)
Query: 386 GANNNDIELDKYKGLILPDAILVKKSYEEKRQKKRGKARSWKLKSLNMEVDDKVARADPD 445
G N + I+LD Y L+ DAI S E++ ++ G + + K+L + + +
Sbjct: 58 GGNWSYIKLDNYTALV--DAIY---SVEDENKQSEGSSNGNRGKNLKVIDSPATLKKTYE 112
Query: 446 KMHSEYEQFLKDLEENPDLRFNISLYRNKEYQPSEIASVTDGEELPSVPL 495
+ ++ +++ P L R ++Y+ S IA +T G E +PL
Sbjct: 113 TRSASSGSSIQVVQKQPQLSNGD---RKRKYK-SRIADITKGSESVKIPL 158
>At5g43560 unknown protein
Length = 1055
Score = 29.3 bits (64), Expect = 5.5
Identities = 24/87 (27%), Positives = 43/87 (48%), Gaps = 6/87 (6%)
Query: 413 EEKRQKKRGKARSWKLKSLNMEVDDKVARADPDKMHSEYEQFLKDLEEN----PDLRFNI 468
E+K +KK+ K + K K M +DKV R ++ E E+ ++ + E+ PD ++
Sbjct: 445 EKKSKKKQAKQKKNKNKGKEMRKEDKV-RTQTEEREIEKEECVRAIAESSAEKPDTLGDV 503
Query: 469 SLYRNKEYQPSEIASVTDGEELPSVPL 495
S + +EI + D E+ S P+
Sbjct: 504 SDVSDSVDSSAEILQL-DSEDRESSPV 529
>At1g11510 hypthetical protein
Length = 352
Score = 29.3 bits (64), Expect = 5.5
Identities = 15/53 (28%), Positives = 31/53 (58%)
Query: 389 NNDIELDKYKGLILPDAILVKKSYEEKRQKKRGKARSWKLKSLNMEVDDKVAR 441
++D EL+K K ++ + + VK++ + K +K G RS ++ + +D K A+
Sbjct: 63 DSDSELEKKKDQVVTNPVDVKRAKKVKTSEKSGAKRSLEVDEAAVSMDVKRAK 115
>At4g35560 hypothetical protein
Length = 917
Score = 28.9 bits (63), Expect = 7.1
Identities = 14/49 (28%), Positives = 27/49 (54%)
Query: 197 KQMDEGIDFYFSNRSHGVKFVEFIGKVAPIKSRSDKQLVSHDTKSNEYN 245
K ++GI S + +F F GK+ + ++++K +V++D K E N
Sbjct: 796 KPKEQGILSGLSKQKMANRFSNFKGKLKQMAAKNEKSVVTNDEKHEEKN 844
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,906,395
Number of Sequences: 26719
Number of extensions: 516092
Number of successful extensions: 1844
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 20
Number of HSP's that attempted gapping in prelim test: 1815
Number of HSP's gapped (non-prelim): 47
length of query: 516
length of database: 11,318,596
effective HSP length: 104
effective length of query: 412
effective length of database: 8,539,820
effective search space: 3518405840
effective search space used: 3518405840
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0065.6