BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0061.9
(277 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g57490 porin-like protein 323 6e-89
At5g15090 voltage-dependent anion-selective channel protein hsr2 241 2e-64
At5g67500 porin-like protein 238 2e-63
At3g01280 putative porin 230 5e-61
At3g49920 porin - like protein 142 2e-34
At5g37610 porin -like protein 29 2.4
At3g57230 MADS-box transcription factor (AGL16) 29 3.2
At5g51920 putative protein 28 4.1
At5g01150 putative protein 28 5.4
At2g34470 putative urease accessory protein 28 7.0
At2g16230 putative beta-1,3-glucanase 27 9.2
At1g22080 hypothetical protein 27 9.2
>At5g57490 porin-like protein
Length = 274
Score = 323 bits (828), Expect = 6e-89
Identities = 164/277 (59%), Positives = 208/277 (74%), Gaps = 3/277 (1%)
Query: 1 MANGPALYPEIGKRARDLLYKDYNFDQKFNLSIPSSTGLGLIATGLKKDQIFIGDINTLY 60
M + PA + +IGK+A+DLL KDY FD KF L++ S+TG +ATGLKKD F GDI+TLY
Sbjct: 1 MGSSPAPFADIGKKAKDLLNKDYIFDHKFTLTMLSATGTEFVATGLKKDDFFFGDISTLY 60
Query: 61 KSGNLTVDVKVDTYSNVSTKVTLNDIFRCKKVALSFNIPDHKSGKLDVQYLHPHAAIDSS 120
K N VD+K+D++S+VSTKVTL ++ K +SF IPDHKSGKLDVQY+HPHA ++SS
Sbjct: 61 KGQNTIVDLKIDSHSSVSTKVTLKNLLPSAKAVISFKIPDHKSGKLDVQYVHPHATLNSS 120
Query: 121 IGLNPSPQLELSTAIGSKDLCMGAEVGFNTTSASFTKYNAGITFNRPDFSAALMLVDKGK 180
IGLNP+P L+LS IGS+++C+G EV F+T S+S TKYNAGI FN SAAL+L DKG+
Sbjct: 121 IGLNPTPLLDLSATIGSQNVCLGGEVSFDTASSSLTKYNAGIGFNNQGVSAALILEDKGE 180
Query: 181 SLKASYIHHVDRPDGFTVAAEINHRFSSFENRFTIGSSQLIDPNTVLKTRFSDDGKAAFL 240
SL+A+Y+H V+ F AE+ RFS++ N FT+GSS +D TV+KTRFS+ GKA +
Sbjct: 181 SLRATYVHTVNPTTSF--GAELIRRFSNYNNSFTVGSSHSVDQFTVVKTRFSNSGKAGMV 238
Query: 241 CQRAWRPKSLITLSAEYDSTKIFGSSTKLGLALALQP 277
QR WRPKS IT SAEYDS K SS KLGLALAL+P
Sbjct: 239 VQREWRPKSHITFSAEYDS-KAVTSSPKLGLALALKP 274
>At5g15090 voltage-dependent anion-selective channel protein hsr2
Length = 274
Score = 241 bits (616), Expect = 2e-64
Identities = 124/277 (44%), Positives = 173/277 (61%), Gaps = 3/277 (1%)
Query: 1 MANGPALYPEIGKRARDLLYKDYNFDQKFNLSIPSSTGLGLIATGLKKDQIFIGDINTLY 60
M GP LY EIGK+ARDLLY+DY DQKF+++ SSTG+ + TG K +F+GD+ T
Sbjct: 1 MVKGPGLYTEIGKKARDLLYRDYQGDQKFSVTTYSSTGVAITTTGTNKGSLFLGDVATQV 60
Query: 61 KSGNLTVDVKVDTYSNVSTKVTLNDIFRCKKVALSFNIPDHKSGKLDVQYLHPHAAIDSS 120
K+ N T DVKV T S++ T +T ++ KV + +PDHKSGK +VQY H +A I +S
Sbjct: 61 KNNNFTADVKVSTDSSLLTTLTFDEPAPGLKVIVQAKLPDHKSGKAEVQYFHDYAGISTS 120
Query: 121 IGLNPSPQLELSTAIGSKDLCMGAEVGFNTTSASFTKYNAGITFNRPDFSAALMLVDKGK 180
+G +P + S +G+ L +G +V +NT S +F +NAG F + D +A+L+L DKG+
Sbjct: 121 VGFTATPIVNFSGVVGTNGLSLGTDVAYNTESGNFKHFNAGFNFTKDDLTASLILNDKGE 180
Query: 181 SLKASYIHHVDRPDGFTVAAEINHRFSSFENRFTIGSSQLIDPNTVLKTRFSDDGKAAFL 240
L ASY V V AEI+H F++ EN T+G+ +DP T +K R ++ G A L
Sbjct: 181 KLNASYYQIVS--PSTVVGAEISHNFTTKENAITVGTQHALDPLTTVKARVNNAGVANAL 238
Query: 241 CQRAWRPKSLITLSAEYDSTKIFGSSTKLGLALALQP 277
Q WRPKS T+S E DS K S K+G+ALAL+P
Sbjct: 239 IQHEWRPKSFFTVSGEVDS-KAIDKSAKVGIALALKP 274
>At5g67500 porin-like protein
Length = 276
Score = 238 bits (608), Expect = 2e-63
Identities = 120/277 (43%), Positives = 175/277 (62%), Gaps = 1/277 (0%)
Query: 1 MANGPALYPEIGKRARDLLYKDYNFDQKFNLSIPSSTGLGLIATGLKKDQIFIGDINTLY 60
M+ GP L+ +IGK+A+DLL +DYN DQKF++S S++G+ L +T LKK + D+ T Y
Sbjct: 1 MSKGPGLFTDIGKKAKDLLTRDYNSDQKFSISTYSASGVALTSTALKKGGVHAADVATQY 60
Query: 61 KSGNLTVDVKVDTYSNVSTKVTLNDIFRCKKVALSFNIPDHKSGKLDVQYLHPHAAIDSS 120
K N DVK+DT S+V T VTL +I K SF +PD+ S KL+VQY H HA + ++
Sbjct: 61 KYKNALFDVKIDTDSSVLTTVTLTEILPSTKAIASFKVPDYNSAKLEVQYFHDHATVTAA 120
Query: 121 IGLNPSPQLELSTAIGSKDLCMGAEVGFNTTSASFTKYNAGITFNRPDFSAALMLVDKGK 180
L +P ++++ +GS + GAE G++TTS +FTKYNAGI+ +PD +++L DKG
Sbjct: 121 AALKQNPLIDITATLGSPVISFGAEAGYDTTSKTFTKYNAGISVTKPDACLSIILGDKGD 180
Query: 181 SLKASYIHHVDRPDGFTVAAEINHRFSSFENRFTIGSSQLIDPNTVLKTRFSDDGKAAFL 240
SLKASY+HH D E+ +FS+ EN T+G ID +T +K + ++ G L
Sbjct: 181 SLKASYLHHFDEFKRTAAVGEVYRKFSTNENTITVGGLYAIDHSTAVKAKLNNHGTLGAL 240
Query: 241 CQRAWRPKSLITLSAEYDSTKIFGSSTKLGLALALQP 277
Q P+SL+T+S+E D TK + GL+LAL+P
Sbjct: 241 LQHEVLPRSLVTVSSEID-TKALEKHPRFGLSLALKP 276
>At3g01280 putative porin
Length = 276
Score = 230 bits (587), Expect = 5e-61
Identities = 120/277 (43%), Positives = 172/277 (61%), Gaps = 1/277 (0%)
Query: 1 MANGPALYPEIGKRARDLLYKDYNFDQKFNLSIPSSTGLGLIATGLKKDQIFIGDINTLY 60
M GP LY EIGK+ARDLLYKD+N DQKF+++ S G+ + +TG KK + +GD+
Sbjct: 1 MVKGPGLYTEIGKKARDLLYKDHNSDQKFSITTFSPAGVAITSTGTKKGDLLLGDVAFQS 60
Query: 61 KSGNLTVDVKVDTYSNVSTKVTLNDIFRCKKVALSFNIPDHKSGKLDVQYLHPHAAIDSS 120
+ N+T D+KV T S T+++ + SF +PD SGK+++QYLH +A I +S
Sbjct: 61 RRKNITTDLKVCTDSTFLITATVDEAAPGLRSIFSFKVPDQNSGKVELQYLHEYAGISTS 120
Query: 121 IGLNPSPQLELSTAIGSKDLCMGAEVGFNTTSASFTKYNAGITFNRPDFSAALMLVDKGK 180
+GL +P + S IGS L +G +V F+T S +FTK NAG++F + D A+L + DKG
Sbjct: 121 MGLTQNPTVNFSGVIGSNVLAVGTDVSFDTKSGNFTKINAGLSFTKEDLIASLTVNDKGD 180
Query: 181 SLKASYIHHVDRPDGFTVAAEINHRFSSFENRFTIGSSQLIDPNTVLKTRFSDDGKAAFL 240
L ASY H V+ V AE++H+ SS ++ T+G+ +DP T +K R + G A+ L
Sbjct: 181 LLNASYYHIVNPLFNTAVGAEVSHKLSSKDSTITVGTQHSLDPLTSVKARVNSAGIASAL 240
Query: 241 CQRAWRPKSLITLSAEYDSTKIFGSSTKLGLALALQP 277
Q W+PKS T+S E D TK S K+GLALAL+P
Sbjct: 241 IQHEWKPKSFFTISGEVD-TKSIDKSAKVGLALALKP 276
>At3g49920 porin - like protein
Length = 226
Score = 142 bits (359), Expect = 2e-34
Identities = 89/277 (32%), Positives = 137/277 (49%), Gaps = 51/277 (18%)
Query: 1 MANGPALYPEIGKRARDLLYKDYNFDQKFNLSIPSSTGLGLIATGLKKDQIFIGDINTLY 60
M+ GP L+ +IGK A+DLL +DY+ DQKF++S S +G+ L +T LK + ++ T Y
Sbjct: 1 MSKGPGLFADIGKYAKDLLTRDYSTDQKFSISTNSVSGVALTSTALKNGVLHAANVATQY 60
Query: 61 KSGNLTVDVKVDTYSNVSTKVTLNDIFRCKKVALSFNIPDHKSGKLDVQYLHPHAAIDSS 120
K N DVK+DT NV + V P +K +D L
Sbjct: 61 KYRNTFFDVKIDTDFNVKSLV----------------YPMNKFVSIDHNTL--------- 95
Query: 121 IGLNPSPQLELSTAIGSKDLCMGAEVGFNTTSASFTKYNAGITFNRPDFSAALMLVDKGK 180
G++TTS +FTKYN G++ +PD +++L DKG
Sbjct: 96 -------------------------TGYDTTSRTFTKYNVGVSVTKPDQCVSIILGDKGD 130
Query: 181 SLKASYIHHVDRPDGFTVAAEINHRFSSFENRFTIGSSQLIDPNTVLKTRFSDDGKAAFL 240
S+KASY++++D E+ + S+ E T+G +D T +K + + +GK L
Sbjct: 131 SIKASYVYYLDESTRSATVGEVIRKISTNETTVTVGGLYAVDHLTNVKAKLNSNGKFGAL 190
Query: 241 CQRAWRPKSLITLSAEYDSTKIFGSSTKLGLALALQP 277
Q PKS++T+S E D TK +LGL+L+L+P
Sbjct: 191 LQHEGLPKSIVTISGEID-TKTLDKYPRLGLSLSLKP 226
>At5g37610 porin -like protein
Length = 163
Score = 29.3 bits (64), Expect = 2.4
Identities = 24/77 (31%), Positives = 35/77 (45%), Gaps = 6/77 (7%)
Query: 137 SKDLCMGAEVGFNTTSASFTKYNAGIT--FNRPDFSAALMLVDKGKSLKASYIHHVDRPD 194
SK +C GAE + + S ++ G F D AL+ V G S++ + HH
Sbjct: 83 SKTVC-GAEAKYILSDKS-NSFSIGKRHLFGDDDLIQALLSVSNGGSMRVLFQHHWTLKS 140
Query: 195 GFTVAAE--INHRFSSF 209
FT+A E +NH F
Sbjct: 141 FFTIAGERLLNHSSQRF 157
>At3g57230 MADS-box transcription factor (AGL16)
Length = 179
Score = 28.9 bits (63), Expect = 3.2
Identities = 14/40 (35%), Positives = 21/40 (52%)
Query: 39 LGLIATGLKKDQIFIGDINTLYKSGNLTVDVKVDTYSNVS 78
L L +KKDQ+ I +I L + GNL +D + V+
Sbjct: 71 LSLRGVRMKKDQMLIEEIQVLNREGNLVHQENLDLHKKVN 110
>At5g51920 putative protein
Length = 570
Score = 28.5 bits (62), Expect = 4.1
Identities = 21/69 (30%), Positives = 34/69 (48%), Gaps = 8/69 (11%)
Query: 39 LGLIATGLKKDQIFIGDINTLYKSGNLTVDVKVDTYSNVSTKVTLNDIFRCKKVALSFNI 98
LGL+ATG + + ++ LYK + T V Y KV N + A++FN+
Sbjct: 408 LGLVATGNRSRCLINWLVSALYKLKHSTTSRLVKIY---GPKVNFN-----RGPAVAFNL 459
Query: 99 PDHKSGKLD 107
+HK K++
Sbjct: 460 FNHKGEKIE 468
>At5g01150 putative protein
Length = 501
Score = 28.1 bits (61), Expect = 5.4
Identities = 20/73 (27%), Positives = 36/73 (48%), Gaps = 10/73 (13%)
Query: 31 LSIPSSTGLGLIATGLKKDQIFIGDINTLYKSGNLTVDVKVDTYSNVSTKVTL------N 84
L++P T + L+ K + + +G N LY+S VD+ D + + K L
Sbjct: 40 LTLPMGTIVRLLENHRKSEPVTLGCFNNLYRS---VVDMGSDCFETEACKQMLVYPKSVK 96
Query: 85 DIFRCKKVALSFN 97
D+ +CK++ L+ N
Sbjct: 97 DL-QCKRLKLNIN 108
>At2g34470 putative urease accessory protein
Length = 275
Score = 27.7 bits (60), Expect = 7.0
Identities = 23/67 (34%), Positives = 32/67 (47%), Gaps = 2/67 (2%)
Query: 113 PHAAIDSSIGLNPSPQLELSTAIGSKDLCMGAEVGFNTTSASFTKYNAGITFNRPDFSAA 172
PHAAI I +N P ELS + DL + E G + +A+F++ A D SA
Sbjct: 139 PHAAIREDISINLGPLEELSN-LFKADLLL-CESGGDNLAANFSRELADYIIYIIDVSAG 196
Query: 173 LMLVDKG 179
+ KG
Sbjct: 197 DKIPRKG 203
>At2g16230 putative beta-1,3-glucanase
Length = 456
Score = 27.3 bits (59), Expect = 9.2
Identities = 17/52 (32%), Positives = 24/52 (45%), Gaps = 6/52 (11%)
Query: 111 LHPHAAIDSSI------GLNPSPQLELSTAIGSKDLCMGAEVGFNTTSASFT 156
L P +ID+ I L P P E S + DL M ++G T++S T
Sbjct: 301 LMPGKSIDTYIFALFDENLKPGPSFEQSFGLFKPDLSMAYDIGLTKTTSSQT 352
>At1g22080 hypothetical protein
Length = 475
Score = 27.3 bits (59), Expect = 9.2
Identities = 11/16 (68%), Positives = 12/16 (74%)
Query: 232 SDDGKAAFLCQRAWRP 247
SDDG+ AFLC R RP
Sbjct: 62 SDDGRVAFLCWRVARP 77
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.318 0.135 0.387
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,009,392
Number of Sequences: 26719
Number of extensions: 248541
Number of successful extensions: 545
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 532
Number of HSP's gapped (non-prelim): 13
length of query: 277
length of database: 11,318,596
effective HSP length: 98
effective length of query: 179
effective length of database: 8,700,134
effective search space: 1557323986
effective search space used: 1557323986
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0061.9