BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0060.3
(388 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g35920 hypothetical protein 44 2e-04
At1g36510 hypothetical protein 42 6e-04
At2g05090 putative replication protein A1 40 0.003
At1g52950 replication protein A1, putative 39 0.004
At3g43100 putative protein 39 0.005
At3g31990 hypothetical protein 39 0.005
At2g11490 pseudogene; similar to MURA transposase of maize Muta... 38 0.011
At5g33240 putative protein 36 0.041
At3g13270 hypothetical protein 35 0.054
At3g42410 putative protein 35 0.092
At3g42980 putative protein 33 0.27
At3g32270 hypothetical protein 32 0.45
At2g14780 putative replication protein A1 32 0.45
At5g35250 putative protein 32 0.59
At4g07440 32 0.59
At1g34710 hypothetical protein 32 0.59
At1g25784 mutator-like transposase, putative 32 0.77
At3g26750 hypothetical protein 31 1.3
At2g11360 putative replication protein A1 30 1.7
At2g07630 putative replication protein A1 30 1.7
>At1g35920 hypothetical protein
Length = 567
Score = 43.9 bits (102), Expect = 2e-04
Identities = 30/130 (23%), Positives = 57/130 (43%), Gaps = 8/130 (6%)
Query: 8 FHTLNQLSLWPHIWTIKVRVICLWKTPEEMLESSHSSLKMILIDREAVKIEAYVCKE--D 65
F L + + + W ++V+++ WK + ++ ++++++ D K+ A V KE
Sbjct: 5 FAYLKDVRPYKNAWRVQVKILHSWK---QYTSNTRETIELVISDEHGKKMHATVKKELVS 61
Query: 66 PITKNIVPGGVYKFSIFQVTCNHGVNRVTNHRYKLLLHSRTMIFPSV---ESRELIVQSI 122
++ G IF +T G R TNH YK+ RT + +S L + S
Sbjct: 62 KFVHKLIVGEWVFIEIFGLTYASGQFRPTNHLYKMAFQVRTEVMGCASVSDSNFLTLASF 121
Query: 123 PNVTKLHINP 132
+ +NP
Sbjct: 122 SKIQSGELNP 131
>At1g36510 hypothetical protein
Length = 351
Score = 42.0 bits (97), Expect = 6e-04
Identities = 65/320 (20%), Positives = 116/320 (35%), Gaps = 45/320 (14%)
Query: 21 WTIKVRVICLWKTPEEMLESSHSSLKMILIDREAVKIEAYVCKEDPITKNIVPGGVYKFS 80
+ IKVRV+ LW+ S++M+L+D E ++ A + E+ + K
Sbjct: 22 YKIKVRVVRLWRL--------FKSIEMVLVDGEGTRVHASI--EEGLGKR---------- 61
Query: 81 IFQVTCNHGVNRVTNHRYKLLLHSRTMIFPSVESRELIVQSIPNVTKLHINPVVEDQSVF 140
FQ G +R+ + + V S + I + KL S
Sbjct: 62 -FQHQFVSGESRIIDTFSFVYYDDVLGQIVDVGSLDSIKAKGKDTVKL---------SAG 111
Query: 141 NERFSGFEIEEPVEYLGYRDSEFNLQDDFIHLHPAKTLLQLIHTEQRGVFIVQARIVSVI 200
+E + E + S L ++F L+ KT+ +++ + AR+ +
Sbjct: 112 SEDLIHVDSENKTSH-----SAVTLYEEFFQLNEKKTVEDIVYACDVSTCVTIARVCFIE 166
Query: 201 KAAGWAYPSCRCYSELKVVNHGYSCSRCCRTMIMIFYFYRYRLNVHVSDGVQSSVFILSD 260
W Y +C+ + + + SC C + Y + V + D
Sbjct: 167 TMPKWYYIACKAHGDAGPI---CSCGVCDADATDLILRASYGASPEVK-------LLFFD 216
Query: 261 PEATHIIKKACEEFAYASPQPLAAHFPPEVEEKLVGAEVLFKVRNDVRRLYDGCFCYDVL 320
A +I K EF P+ PEV L+G +LFK+ L Y V
Sbjct: 217 GLAQCLIGKTAAEFFAEVPKESDPSILPEVLADLMGKTMLFKLSIGTDNLKSTKAAYVVE 276
Query: 321 HICSDSETMDKFSAKRTSVT 340
++ ++KF+ + T V+
Sbjct: 277 KFWEKTDMVEKFAKELTVVS 296
>At2g05090 putative replication protein A1
Length = 521
Score = 39.7 bits (91), Expect = 0.003
Identities = 33/155 (21%), Positives = 66/155 (42%), Gaps = 14/155 (9%)
Query: 173 HPAKTLLQLIHTEQRGVFIVQARIVSVIKAAGWAYPSC-RCYSELKVVNHGYSCSRCCRT 231
+PAKT++ L++ G + + I ++ GW Y C +C ++++V S + +
Sbjct: 220 YPAKTIVDLLNGRDVGQWRILGSIFAIDTDWGWFYFGCPKCNRKIELVKESTSTVKRIQA 279
Query: 232 MIMIFYFY------------RYRLNVHVSDGVQSSVFILSDPEATHIIKKACEEFAYASP 279
++ RY+L++ V D ++ + AT++I+K+ EE
Sbjct: 280 PTKPKFWCDKYQESITNVEARYKLHIRVMDQTGEIKLMVFENNATNLIRKSSEELLDGQY 339
Query: 280 QPLA-AHFPPEVEEKLVGAEVLFKVRNDVRRLYDG 313
+ + P+V L G F V + +Y G
Sbjct: 340 EEIEDPTIKPDVITNLCGKTFHFLVSVEKANIYGG 374
>At1g52950 replication protein A1, putative
Length = 566
Score = 39.3 bits (90), Expect = 0.004
Identities = 28/104 (26%), Positives = 48/104 (45%), Gaps = 5/104 (4%)
Query: 5 VIPFHTLNQLSLWPHIWTIKVRVICLWKTPEEMLESSHSSLKMILIDREAVKIEAYVCKE 64
V P+ + L + W I+V+++ W + S S +M+L D + KI+A + KE
Sbjct: 4 VNPYTAIKNLKPFKTSWCIQVKILHAWN---HYTKGSGMSYEMMLADEDGNKIQAGIKKE 60
Query: 65 D--PITKNIVPGGVYKFSIFQVTCNHGVNRVTNHRYKLLLHSRT 106
+ + + G F VT G+ R T H Y++ + S T
Sbjct: 61 HLLKLQRYVKIGHWTIIEEFSVTKASGLYRSTTHPYRINIQSST 104
>At3g43100 putative protein
Length = 269
Score = 38.9 bits (89), Expect = 0.005
Identities = 29/108 (26%), Positives = 56/108 (51%), Gaps = 7/108 (6%)
Query: 6 IPFHTLNQLSLWPHIWTIKVRVICLWKTPEEMLESSHSSLKMILIDREAVKIEAYVCKED 65
+PF L L + + W I+V+++ +W+ + + S++MIL+D K+ A V +E
Sbjct: 7 LPFTPLKSLKPYKNAWRIQVKLLHVWR---QYSVKAGESIEMILVDEAGDKMYAAVRREQ 63
Query: 66 -PITKNIVPGGVYKFSIFQVTCN--HGVNRVTNHRYKLLLHSRTMIFP 110
+ + GV+K I +T N G R+++ +YK+ +T + P
Sbjct: 64 IKKFERCLTEGVWKI-ITTITLNPTSGKYRISDLKYKIGFVFKTTVSP 110
>At3g31990 hypothetical protein
Length = 450
Score = 38.9 bits (89), Expect = 0.005
Identities = 29/104 (27%), Positives = 47/104 (44%), Gaps = 5/104 (4%)
Query: 7 PFHTLNQLSLWPHIWTIKVRVICLWKTPEEMLESSHSSLKMILIDREAVKIEAYVCKE-- 64
P L+ + + W ++VRV+ WKT + M+L D KI A V +E
Sbjct: 4 PIAYLDDIKPYKTYWRVQVRVLHTWKT---FTMQFGETFDMVLSDVRGKKIHASVKREHL 60
Query: 65 DPITKNIVPGGVYKFSIFQVTCNHGVNRVTNHRYKLLLHSRTMI 108
+ + IV G F +T G + T+HRYK+ ++T +
Sbjct: 61 NRFERPIVRGEWRAIENFGLTYATGQYKATDHRYKMGFMAQTHV 104
>At2g11490 pseudogene; similar to MURA transposase of maize Mutator
transposon
Length = 240
Score = 37.7 bits (86), Expect = 0.011
Identities = 31/123 (25%), Positives = 57/123 (46%), Gaps = 13/123 (10%)
Query: 5 VIPFHTLNQLSLWPHIWTIKVRVICLWKTPEEMLESSHSSLKMILIDREAVKIEAYVCKE 64
+I FH L +LS W I V V+ ++K ++L L+++L+D+ +IEA +
Sbjct: 1 MIQFHRLYELSPLVTGWAICVMVVRVYK---KILNHDAFELRLLLVDKLGTQIEATIGPR 57
Query: 65 ------DPITKNIVPGGVYKFSIFQVTCNHGVNRVTNHRYKLLLHSRTMIFPSVESRELI 118
D I +N + F V N R T H +++L +T++ PS ++
Sbjct: 58 FSAFYYDRIKENQWK----TITTFIVRPNCEAIRTTPHEFRILFMEQTVVTPSTTKETVV 113
Query: 119 VQS 121
+ +
Sbjct: 114 LNN 116
>At5g33240 putative protein
Length = 192
Score = 35.8 bits (81), Expect = 0.041
Identities = 34/120 (28%), Positives = 58/120 (48%), Gaps = 10/120 (8%)
Query: 21 WTIKVRVICLWKTPEEMLESSHSSLKMILIDREAVKIEAYVCKEDPITK--NIVPGGVYK 78
W ++V+V+ W M + +LK++L++ + VKI A V K+D ++K N +P +
Sbjct: 18 WRVQVKVLHSWCQYTNM---TGETLKLVLVNSQGVKIHASV-KKDLVSKYVNNLPLNEWT 73
Query: 79 F-SIFQVTCNHGVNRVTNHRYKLLLHSRTMIF--PSV-ESRELIVQSIPNVTKLHINPVV 134
F F + G R T H YK+ + T + SV S L + + N+ NP +
Sbjct: 74 FIETFALNHASGQFRPTGHLYKMSFVTGTQVIRCDSVSNSSYLSLATFANIQTGDSNPAI 133
>At3g13270 hypothetical protein
Length = 570
Score = 35.4 bits (80), Expect = 0.054
Identities = 36/164 (21%), Positives = 60/164 (35%), Gaps = 16/164 (9%)
Query: 173 HPAKTLLQLIHTEQRGVFIVQARIVSVIKAAGWAYPSCR-CYSELKVVNHG--------- 222
+P T+ QL++ G + I ++ W Y SCR C ++ ++ G
Sbjct: 258 YPRNTIQQLLNAVDVGKARLMCTIYAIDTDWAWYYISCRACNKKVTHIHSGVHGVNNKGK 317
Query: 223 ---YSCSRCCRTMIMIFYFYRYRLNVHVSDGVQSSVFILSDPEATHIIKKACEEFAYASP 279
+ C C ++ RY + V D S +L D T II + S
Sbjct: 318 KPRFWCDSC--KTVVTNVIARYMIYAKVMDNTGESKLLLFDQICTEIIGETAPSVLNGSV 375
Query: 280 QPL-AAHFPPEVEEKLVGAEVLFKVRNDVRRLYDGCFCYDVLHI 322
+ P+ + L G LF V + ++DG Y V +
Sbjct: 376 DEIDDPDDLPDALKNLYGKTFLFLVSIEKENIWDGKEIYKVTKV 419
Score = 34.3 bits (77), Expect = 0.12
Identities = 27/120 (22%), Positives = 57/120 (47%), Gaps = 10/120 (8%)
Query: 21 WTIKVRVICLWKTPEEMLESSHSSLKMILIDREAVKIEAYVCKEDPITK--NIVPGGVYK 78
W ++V+ + W+ + ++ +L+++ D KI V ++D ++K N++ G +
Sbjct: 18 WRVQVKTLHAWR---QYTANTGETLEVVFSDETGKKIHCSV-RKDLVSKYANMLTVGEWV 73
Query: 79 F-SIFQVTCNHGVNRVTNHRYKLLLHSRTMIFPS---VESRELIVQSIPNVTKLHINPVV 134
F F + G R T+H YK+ + T + PS +S L + + + +NP +
Sbjct: 74 FIETFSLNYAGGSFRPTSHLYKMSFVNGTSVIPSPSVSDSNYLTLATFEKIQSGELNPTM 133
>At3g42410 putative protein
Length = 474
Score = 34.7 bits (78), Expect = 0.092
Identities = 26/111 (23%), Positives = 50/111 (44%), Gaps = 6/111 (5%)
Query: 21 WTIKVRVICLWKTPEEMLESSHSSLKMILIDREAVKIEAYV---CKEDPITKNIVPGGVY 77
W + V+++ LW P MIL D + KI+A K++ + + G Y
Sbjct: 278 WFVFVKLLRLWNWPPNY---DGEEPTMILADEKGNKIDANSPLHVKKEVLISTLKEGQWY 334
Query: 78 KFSIFQVTCNHGVNRVTNHRYKLLLHSRTMIFPSVESRELIVQSIPNVTKL 128
S F+V + ++R +NH + H+ T+ V I++++ + K+
Sbjct: 335 LLSHFEVAVANPLDRFSNHPLYRIKHASTVEKQFVVDAMGIIEAVSPIGKI 385
>At3g42980 putative protein
Length = 303
Score = 33.1 bits (74), Expect = 0.27
Identities = 25/95 (26%), Positives = 41/95 (42%), Gaps = 5/95 (5%)
Query: 8 FHTLNQLSLWPHIWTIKVRVICLWKTPEEMLESSHSSLKMILIDREAVKIEAYVCKE--D 65
F L + + W I+V+V W+ M + +L+++L+D VKI A V K+
Sbjct: 5 FAFLRDIRPYKTSWRIQVKVFHSWRQYTSM---TGETLELVLVDAHGVKIHASVKKDLVS 61
Query: 66 PITKNIVPGGVYKFSIFQVTCNHGVNRVTNHRYKL 100
N++ F + G R T H YK+
Sbjct: 62 NFVNNLLLNEWRFIETFALNHASGQFRPTGHLYKM 96
>At3g32270 hypothetical protein
Length = 209
Score = 32.3 bits (72), Expect = 0.45
Identities = 26/119 (21%), Positives = 54/119 (44%), Gaps = 10/119 (8%)
Query: 21 WTIKVRVICLWKTPEEMLESSHSSLKMILIDREAVKIEAYVCKE--DPITKNIVPGGVYK 78
W +++RV+ ++ L + + ++L D V ++A + KE D K I G
Sbjct: 17 WYVRLRVV---RSLVVHLNALSKIVGLVLADEHGVMVDAMIEKEFADYYNKFIDAGDWIT 73
Query: 79 FSIFQVTCNHGVNRVTNHRYKLLLHSRTMIFPSVESRELIVQSIPNVTKLHINPVVEDQ 137
F V N RVT+H++K+ T++ + V + P+ + + +++D+
Sbjct: 74 IMRFGVYPNLNPVRVTSHKFKICFFKDTVV-----RKTTAVVANPHYALTYFSSIIDDE 127
>At2g14780 putative replication protein A1
Length = 235
Score = 32.3 bits (72), Expect = 0.45
Identities = 29/103 (28%), Positives = 52/103 (50%), Gaps = 5/103 (4%)
Query: 8 FHTLNQLSLWPHIWTIKVRVICLWKTPEEMLESSHSSLKMILIDREAVKIEAYVCKE-DP 66
FHT+++L W I V V+ ++K +++ + L +IL D E +IEA V + P
Sbjct: 4 FHTVSELCPLITGWRIYVFVVRVFK---KVISPNVFELGLILADYENNRIEATVDRRLAP 60
Query: 67 ITKNIVPGGVYK-FSIFQVTCNHGVNRVTNHRYKLLLHSRTMI 108
++ +K + F V + + R T H+Y +L RT++
Sbjct: 61 FYEDRFVENEWKTITSFLVRKSTDLVRATKHKYGILFMDRTIV 103
>At5g35250 putative protein
Length = 384
Score = 32.0 bits (71), Expect = 0.59
Identities = 16/59 (27%), Positives = 33/59 (55%), Gaps = 3/59 (5%)
Query: 6 IPFHTLNQLSLWPHIWTIKVRVICLWKTPEEMLESSHSSLKMILIDREAVKIEAYVCKE 64
+PF L + + + W I+V+++ +W+ + + S++MIL+D K+ A V +E
Sbjct: 7 LPFTPLKSVKPYKNAWRIQVKLLHVWR---QYSVKAGESIEMILVDEAGDKMYAAVRRE 62
>At4g07440
Length = 261
Score = 32.0 bits (71), Expect = 0.59
Identities = 26/109 (23%), Positives = 47/109 (42%), Gaps = 8/109 (7%)
Query: 9 HTLNQLSLWPHIWTIKVRVICLWKTPEEMLESSHSSL-KMILIDREAVKIEAYVCK---E 64
H L L W I V+++ +W P +SH + MIL D + +++A + +
Sbjct: 4 HKLTDLRDTSTNWKINVKILSIWNHP----PNSHGEITTMILHDDKNNRVDATIPQGNYH 59
Query: 65 DPITKNIVPGGVYKFSIFQVTCNHGVNRVTNHRYKLLLHSRTMIFPSVE 113
+P + PG S F+V R ++ R+ + T ++P E
Sbjct: 60 NPFRPFLKPGTWIHISGFRVVVPQSRVRYSSFRFHIKFIWETSVYPLPE 108
>At1g34710 hypothetical protein
Length = 235
Score = 32.0 bits (71), Expect = 0.59
Identities = 29/103 (28%), Positives = 51/103 (49%), Gaps = 5/103 (4%)
Query: 8 FHTLNQLSLWPHIWTIKVRVICLWKTPEEMLESSHSSLKMILIDREAVKIEAYVCKE-DP 66
FHT+++L W I V V+ ++K +++ + L +IL D E +IEA V + P
Sbjct: 4 FHTVSELCPLITRWRIYVFVVRVFK---KVISPNVFELGLILADYENNRIEATVDRRLAP 60
Query: 67 ITKNIVPGGVYK-FSIFQVTCNHGVNRVTNHRYKLLLHSRTMI 108
++ +K + F V + + R T H Y +L RT++
Sbjct: 61 FYEDRFVENEWKTITSFLVRKSTDLVRATKHEYGILFMDRTVV 103
>At1g25784 mutator-like transposase, putative
Length = 1127
Score = 31.6 bits (70), Expect = 0.77
Identities = 29/103 (28%), Positives = 51/103 (49%), Gaps = 5/103 (4%)
Query: 8 FHTLNQLSLWPHIWTIKVRVICLWKTPEEMLESSHSSLKMILIDREAVKIEAYVCKE-DP 66
FHT+++L W I V V+ ++K +++ + L +IL D E +IEA V + P
Sbjct: 923 FHTVSELCPLITGWRIYVFVVRVFK---KVISPNVFELGLILADYENNRIEATVDRRLAP 979
Query: 67 ITKNIVPGGVYK-FSIFQVTCNHGVNRVTNHRYKLLLHSRTMI 108
++ +K + F V + + R T H Y +L RT++
Sbjct: 980 FYEDRFVENEWKTITSFLVRKSTDLVRATKHEYGILFMDRTVV 1022
>At3g26750 hypothetical protein
Length = 515
Score = 30.8 bits (68), Expect = 1.3
Identities = 16/46 (34%), Positives = 26/46 (55%)
Query: 325 DSETMDKFSAKRTSVTLVESKSDSRVTYLEEDNKQAVCVVEKVGLL 370
DS+T K K +V+ + KS VT++ E++++A E V LL
Sbjct: 30 DSKTNPKIHCKSLNVSTIVGKSQLLVTWINEEDEEAASKEEIVSLL 75
>At2g11360 putative replication protein A1
Length = 145
Score = 30.4 bits (67), Expect = 1.7
Identities = 23/97 (23%), Positives = 42/97 (42%), Gaps = 13/97 (13%)
Query: 21 WTIKVRVICLWKTPEEMLESSHSSLKMILIDREAVKIEAYV------CKEDPITKNIVPG 74
WTI V V+ +T + + L+++ +D ++IEA + C D + +N
Sbjct: 17 WTICVMVL---RTYKRFYRDNAFELRVVFVDEWGIQIEAIIERYFSQCYFDKLKENQWK- 72
Query: 75 GVYKFSIFQVTCNHGVNRVTNHRYKLLLHSRTMIFPS 111
+ + F+V N G+ R H Y + T + S
Sbjct: 73 ---EIATFRVIPNSGIVRAITHYYMIEFMEATSVISS 106
>At2g07630 putative replication protein A1
Length = 458
Score = 30.4 bits (67), Expect = 1.7
Identities = 21/64 (32%), Positives = 32/64 (49%), Gaps = 2/64 (3%)
Query: 47 MILIDREAVKIEAYVCKE--DPITKNIVPGGVYKFSIFQVTCNHGVNRVTNHRYKLLLHS 104
M+L D KI A V +E + + IVPG F +T G + T+HRYK+ +
Sbjct: 9 MVLSDVRGKKIHASVKREHLNRFERLIVPGEWRAIENFGLTYATGQFKATDHRYKMGFMA 68
Query: 105 RTMI 108
+T +
Sbjct: 69 QTRV 72
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.322 0.137 0.417
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,902,580
Number of Sequences: 26719
Number of extensions: 373482
Number of successful extensions: 975
Number of sequences better than 10.0: 31
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 26
Number of HSP's that attempted gapping in prelim test: 963
Number of HSP's gapped (non-prelim): 35
length of query: 388
length of database: 11,318,596
effective HSP length: 101
effective length of query: 287
effective length of database: 8,619,977
effective search space: 2473933399
effective search space used: 2473933399
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0060.3