BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0058a.4
(457 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g04920 unknown protein 64 2e-10
At4g18870 heat shock transcription factor - like protein 35 0.086
At4g34810 putative protein 33 0.33
At2g12520 pseudogene 31 1.6
At1g59540 zcf125 kinesin-like protein 31 1.6
At4g38850 small auxin up RNA (SAUR-AC1) 30 2.1
At4g34790 putative protein 30 3.6
At5g55340 unknown protein 29 4.7
At3g03840 putative auxin-induced protein 29 4.7
At2g17620 putative cyclin 2 29 4.7
At1g72860 unknown protein 29 4.7
At5g55830 serine/threonine-specific kinase like protein 28 8.0
At5g27890 putative protein 28 8.0
At4g38840 auxin-induced protein - like 28 8.0
At4g34800 putative protein 28 8.0
>At4g04920 unknown protein
Length = 1196
Score = 63.9 bits (154), Expect = 2e-10
Identities = 31/59 (52%), Positives = 41/59 (68%)
Query: 66 QLLCGSKGDEEPPLGRSRTGAKNAGQGYTFEEVKIFFLALLDLCRQISRLQHPLPVSQV 124
QL G KG +E ++ G+ NAGQGYT+EEV++ F L+DLC++ S L HPLP SQV
Sbjct: 960 QLGTGVKGIDENSARTTKMGSGNAGQGYTYEEVRVLFHILMDLCKRTSGLAHPLPGSQV 1018
>At4g18870 heat shock transcription factor - like protein
Length = 291
Score = 35.0 bits (79), Expect = 0.086
Identities = 35/130 (26%), Positives = 60/130 (45%), Gaps = 12/130 (9%)
Query: 143 WEFKDFVNKRQEQFLWHRPAGNTLISCHFWPHGSRGLIFHGDSITGQVKDLWDHYMFGNR 202
W ++F N ++F + R NT S F HG R + DS G+ + D+++ G R
Sbjct: 43 WNPEEFYNNLLQRFCFQRI--NTFFSFLF-SHGFRKI----DS--GKWEFANDNFVRGQR 93
Query: 203 EYYCMLLASWLEEEQVLELLSNLLKAQDLLSAQAKRLQSQLDLLAGHWENPQSQLPKSRP 262
+++ +E+E + L KA+ L + K++Q Q L H P S+LP
Sbjct: 94 HLINNIISDVIEQEVQYDQGMELFKAEKLFARLVKKVQDQ---LPPHNSYPTSKLPFPTK 150
Query: 263 LIEVPKQKSN 272
+ E+ S+
Sbjct: 151 IYEMVDDPSS 160
>At4g34810 putative protein
Length = 105
Score = 33.1 bits (74), Expect = 0.33
Identities = 13/39 (33%), Positives = 25/39 (63%)
Query: 131 KMYVVLQAWIQHWEFKDFVNKRQEQFLWHRPAGNTLISC 169
K +VV +++ H FK+F+++ +E+F ++ P G I C
Sbjct: 53 KRFVVPISFLNHPSFKEFLSRAEEEFGFNHPMGGLTIPC 91
>At2g12520 pseudogene
Length = 356
Score = 30.8 bits (68), Expect = 1.6
Identities = 28/103 (27%), Positives = 49/103 (47%), Gaps = 15/103 (14%)
Query: 202 REYYCMLLASWLEEEQVLELLSNLLKAQDLLSAQAKRLQSQLDLLAGHWENPQ-SQLPKS 260
++ Y ML E E L L+ + KA L+ + RLQ++LD L H ++ + S S
Sbjct: 239 KKEYTMLEGQAAEVESNLALIDQITKAAIDLTVERPRLQAELDDLEAHCKSKKVSDFTLS 298
Query: 261 RPLIEVPK-------QKSNFDSHGLLKVVMTIGMEEYELKKKA 296
+ +++P+ + + D G M IG++E+ K A
Sbjct: 299 K--LDIPQVSEISVVRPMDVDEQG-----MPIGLDEFGSNKDA 334
>At1g59540 zcf125 kinesin-like protein
Length = 823
Score = 30.8 bits (68), Expect = 1.6
Identities = 27/127 (21%), Positives = 57/127 (44%), Gaps = 10/127 (7%)
Query: 183 GDSITGQVKDLWDHYMFGNREYYCMLLASWLEEEQVLELLSNLLKAQDLLSAQAKRLQSQ 242
G SI + D W+ + +LL+ ++ E L++ K Q+ + + K+L+S+
Sbjct: 646 GSSIDHPLSDHWETLRVNLKNTTTLLLSDAQAKD---EFLNSHNKGQETAALEEKKLKSE 702
Query: 243 LDLLAGHWENPQSQLPKSRPLIEVPKQKSNFDSHGLLKVVMTIGMEEYELKKKALSVPQQ 302
L ++ + + +L + L+E ++ L+K V + E L +K + Q
Sbjct: 703 LIIIKERYNELEKELCLDKQLLEASRE----SHEKLIKEVQFLKEERDSLDRK---ISQS 755
Query: 303 NQPLFIL 309
Q L ++
Sbjct: 756 TQRLRVI 762
>At4g38850 small auxin up RNA (SAUR-AC1)
Length = 89
Score = 30.4 bits (67), Expect = 2.1
Identities = 13/40 (32%), Positives = 22/40 (54%)
Query: 131 KMYVVLQAWIQHWEFKDFVNKRQEQFLWHRPAGNTLISCH 170
K YVV +++ F+ ++K +E+F + P G I CH
Sbjct: 38 KRYVVPVSYLNQPLFQQLLSKSEEEFGYDHPMGGLTIPCH 77
>At4g34790 putative protein
Length = 108
Score = 29.6 bits (65), Expect = 3.6
Identities = 12/39 (30%), Positives = 23/39 (58%)
Query: 131 KMYVVLQAWIQHWEFKDFVNKRQEQFLWHRPAGNTLISC 169
K +VV +++ H F++F+N+ +E+ +H G I C
Sbjct: 55 KRFVVPISYLNHPLFREFLNRAEEECGFHHSMGGLTIPC 93
>At5g55340 unknown protein
Length = 333
Score = 29.3 bits (64), Expect = 4.7
Identities = 17/72 (23%), Positives = 35/72 (48%)
Query: 269 QKSNFDSHGLLKVVMTIGMEEYELKKKALSVPQQNQPLFILFLEGNELLVQIPQVMTAIK 328
Q++ + L K+V I + + + S Q P +L L L ++I ++T +K
Sbjct: 105 QQNPKSQNHLPKLVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVK 164
Query: 329 VLLLLEMGCQTD 340
V++ + +GC +
Sbjct: 165 VVVFISLGCDLE 176
>At3g03840 putative auxin-induced protein
Length = 95
Score = 29.3 bits (64), Expect = 4.7
Identities = 12/37 (32%), Positives = 22/37 (59%)
Query: 133 YVVLQAWIQHWEFKDFVNKRQEQFLWHRPAGNTLISC 169
Y+VL +++ F+D ++K +E+F + P G I C
Sbjct: 45 YLVLVSYLSQPLFQDLLSKSEEEFGFDHPMGGLTIPC 81
>At2g17620 putative cyclin 2
Length = 429
Score = 29.3 bits (64), Expect = 4.7
Identities = 24/77 (31%), Positives = 34/77 (43%), Gaps = 2/77 (2%)
Query: 252 NPQSQLPKSRPLIEVPKQKSNFDSHGLLKVVMTIGMEEYELKKKALSVPQQNQPLFILFL 311
N + L ++ E QK FDS L V G+EE KK SVP N +F+
Sbjct: 58 NKRGSLLSNKQEEEEGCQKKKFDS--LRPSVTRSGVEEETNKKLKPSVPSANDFGDCIFI 115
Query: 312 EGNELLVQIPQVMTAIK 328
+ E + +P M+ K
Sbjct: 116 DEEEATLDLPMPMSLEK 132
>At1g72860 unknown protein
Length = 1337
Score = 29.3 bits (64), Expect = 4.7
Identities = 45/171 (26%), Positives = 70/171 (40%), Gaps = 23/171 (13%)
Query: 205 YCMLLASWLEEEQVLELL-----SNLLKAQDLLSAQ-AKRLQSQLDLLAGHWEN-PQSQL 257
Y + L + L+ L SN +K++ L S L S+L LL HW+ P +
Sbjct: 543 YALHLGGYTAYHDTLKFLKIYKHSNHIKSKLLFSGDDTNLLSSRLRLL--HWDAFPLTTF 600
Query: 258 P---KSRPLIEVPKQKSNFDSHGLLKVVMTIG----MEEYELKKKALSV-------PQQN 303
P + + L+E+ +SN S VV + + Y L L++ Q
Sbjct: 601 PCRFQPQDLVEIILHRSNLTSFWKETVVKALNRSMLITMYLLVLNILAIFLFFVEYAQGM 660
Query: 304 QPLFILFLEGNELLVQIPQVMTAIKVLLLLEMGCQTDTWNPGGCSDLTATT 354
L L L +E L Q+P + A+ + L+ GC+ P S LT T
Sbjct: 661 PNLRRLDLSDSENLEQLPDLSMAVNLEELITQGCKRLKKIPESISYLTRLT 711
>At5g55830 serine/threonine-specific kinase like protein
Length = 681
Score = 28.5 bits (62), Expect = 8.0
Identities = 14/39 (35%), Positives = 18/39 (45%)
Query: 158 WHRPAGNTLISCHFWPHGSRGLIFHGDSITGQVKDLWDH 196
W G L+ F P+GS I + +S TG V W H
Sbjct: 428 WCNEKGELLLVYEFMPNGSLDKILYQESQTGAVALDWSH 466
>At5g27890 putative protein
Length = 799
Score = 28.5 bits (62), Expect = 8.0
Identities = 14/30 (46%), Positives = 19/30 (62%)
Query: 215 EEQVLELLSNLLKAQDLLSAQAKRLQSQLD 244
EEQ ELL +L K+ + A+ RL+S LD
Sbjct: 516 EEQARELLESLKKSDNFSRAEQSRLRSSLD 545
>At4g38840 auxin-induced protein - like
Length = 99
Score = 28.5 bits (62), Expect = 8.0
Identities = 12/39 (30%), Positives = 21/39 (53%)
Query: 131 KMYVVLQAWIQHWEFKDFVNKRQEQFLWHRPAGNTLISC 169
K +VV +++ F+D + K +E+F + P G I C
Sbjct: 48 KRFVVPVSYLDQPSFQDLLRKAEEEFGFDHPMGGLTIPC 86
>At4g34800 putative protein
Length = 94
Score = 28.5 bits (62), Expect = 8.0
Identities = 11/39 (28%), Positives = 23/39 (58%)
Query: 131 KMYVVLQAWIQHWEFKDFVNKRQEQFLWHRPAGNTLISC 169
K +VV +++ H F+ +++ +E+F ++ P G I C
Sbjct: 38 KRFVVPISYLNHPSFQGLLSRAEEEFGFNHPIGGLTIPC 76
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.320 0.138 0.429
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,816,500
Number of Sequences: 26719
Number of extensions: 476551
Number of successful extensions: 1153
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 1141
Number of HSP's gapped (non-prelim): 16
length of query: 457
length of database: 11,318,596
effective HSP length: 103
effective length of query: 354
effective length of database: 8,566,539
effective search space: 3032554806
effective search space used: 3032554806
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0058a.4