BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0048.5
(117 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g26731 unknown protein 80 2e-16
At3g59860 putative protein 29 0.43
At5g16690 unknown protein 28 0.73
At4g08890 putative transposon protein 28 0.73
At1g30980 F17F8.10 28 0.96
At5g27590 putative protein 27 1.3
At3g28800 hypothetical protein 27 1.6
At2g18500 unknown protein (At2g18500) 27 1.6
At1g55400 hypothetical protein 27 1.6
At5g40110 putative protein 26 2.8
At5g07780 putative protein 26 2.8
At5g03950 putative protein 26 2.8
At1g69240 alpha/beta hydrolase like protein 26 2.8
At1g04730 hypothetical protein 25 4.8
At5g64190 putative protein 25 6.2
At5g07930 putative protein 25 6.2
At1g23830 unknown protein 25 6.2
At5g59200 putative protein 25 8.1
At5g24560 phloem-specific lectin-like protein 25 8.1
At3g59850 polygalacturonase-like protein 25 8.1
>At5g26731 unknown protein
Length = 99
Score = 79.7 bits (195), Expect = 2e-16
Identities = 48/96 (50%), Positives = 59/96 (61%), Gaps = 8/96 (8%)
Query: 10 LFRSESLGHYYDYNYYPDQNYTMMEKRQLFLRSYQFCQKKSFTERIKGSLIRAKKVLWLR 69
+FRS S G YY Y MEKRQLFLRSYQF +K+SF E++ S+ R K+V+ R
Sbjct: 6 VFRSTSGGDYYGAPYGE-----YMEKRQLFLRSYQFSRKQSFAEKVSRSVTRVKRVVLTR 60
Query: 70 LWSSSAGGFRKLVFFPRFRCTFYYRRRRFSRLLNRH 105
L S+ K V + R R FYYRRRRF RLL+ H
Sbjct: 61 LRSAPK---LKRVVWSRLRSAFYYRRRRFFRLLHLH 93
>At3g59860 putative protein
Length = 129
Score = 28.9 bits (63), Expect = 0.43
Identities = 11/25 (44%), Positives = 18/25 (72%)
Query: 4 VTVNPSLFRSESLGHYYDYNYYPDQ 28
+T++P++ SES G YDYN + D+
Sbjct: 70 ITIDPTIDGSESSGEDYDYNKWNDE 94
>At5g16690 unknown protein
Length = 734
Score = 28.1 bits (61), Expect = 0.73
Identities = 16/47 (34%), Positives = 24/47 (51%), Gaps = 5/47 (10%)
Query: 26 PDQNYTMMEKRQLFLRSYQFCQKKSFTERIKGSLIRAKKVLWLRLWS 72
P +T+ K LF+RSY CQ + T S +R K+ L+ +S
Sbjct: 318 PCSGFTVSHKVALFMRSYFLCQDGTLT-----SFVRTLKIACLQHFS 359
>At4g08890 putative transposon protein
Length = 1028
Score = 28.1 bits (61), Expect = 0.73
Identities = 14/42 (33%), Positives = 21/42 (49%)
Query: 5 TVNPSLFRSESLGHYYDYNYYPDQNYTMMEKRQLFLRSYQFC 46
T+ P+ SE +G YDYN + D + T + F R + C
Sbjct: 134 TLYPTTDGSEGIGEEYDYNKWNDPHSTDCSTSREFSRLLETC 175
>At1g30980 F17F8.10
Length = 242
Score = 27.7 bits (60), Expect = 0.96
Identities = 11/24 (45%), Positives = 17/24 (70%)
Query: 5 TVNPSLFRSESLGHYYDYNYYPDQ 28
T++P++ SES G YDYN + D+
Sbjct: 123 TIDPTIDGSESSGEDYDYNKWNDE 146
>At5g27590 putative protein
Length = 320
Score = 27.3 bits (59), Expect = 1.3
Identities = 12/27 (44%), Positives = 17/27 (62%)
Query: 1 MASVTVNPSLFRSESLGHYYDYNYYPD 27
M T++P++ SES G YDYN + D
Sbjct: 178 MGGSTLDPTIDGSESSGEDYDYNKWND 204
>At3g28800 hypothetical protein
Length = 317
Score = 26.9 bits (58), Expect = 1.6
Identities = 12/27 (44%), Positives = 17/27 (62%)
Query: 1 MASVTVNPSLFRSESLGHYYDYNYYPD 27
M T++P++ SES G YDYN + D
Sbjct: 182 MEGSTLDPTIDGSESSGEDYDYNKWND 208
>At2g18500 unknown protein (At2g18500)
Length = 315
Score = 26.9 bits (58), Expect = 1.6
Identities = 20/75 (26%), Positives = 35/75 (46%), Gaps = 7/75 (9%)
Query: 25 YPDQNYTMME---KRQLF----LRSYQFCQKKSFTERIKGSLIRAKKVLWLRLWSSSAGG 77
Y D +MME ++++F L C +R +++RA +W+ L+S +GG
Sbjct: 238 YEDFKGSMMEMIVEKKMFEVAELEQLLSCFLSLNAKRHHRAIVRAFSEIWVALFSGGSGG 297
Query: 78 FRKLVFFPRFRCTFY 92
R+ F R + Y
Sbjct: 298 GRRSSSFSSVRLSDY 312
>At1g55400 hypothetical protein
Length = 211
Score = 26.9 bits (58), Expect = 1.6
Identities = 12/27 (44%), Positives = 17/27 (62%)
Query: 1 MASVTVNPSLFRSESLGHYYDYNYYPD 27
M T++P++ SES G YDYN + D
Sbjct: 152 MGGSTLDPTIDGSESNGEDYDYNKWND 178
>At5g40110 putative protein
Length = 280
Score = 26.2 bits (56), Expect = 2.8
Identities = 11/23 (47%), Positives = 16/23 (68%)
Query: 5 TVNPSLFRSESLGHYYDYNYYPD 27
T++P++ SES G YDYN + D
Sbjct: 160 TLDPTIDGSESSGEDYDYNKWND 182
>At5g07780 putative protein
Length = 464
Score = 26.2 bits (56), Expect = 2.8
Identities = 13/52 (25%), Positives = 30/52 (57%), Gaps = 1/52 (1%)
Query: 8 PSLFRSESLGHYYDYNY-YPDQNYTMMEKRQLFLRSYQFCQKKSFTERIKGS 58
P+ +E+L HY+ Y+Y + T++ +LF ++++ K++ E+ K +
Sbjct: 404 PARGNAEALAHYFGYHYPFEQVTATLLSFIRLFKKAHEENVKQAELEKKKAA 455
>At5g03950 putative protein
Length = 252
Score = 26.2 bits (56), Expect = 2.8
Identities = 11/23 (47%), Positives = 16/23 (68%)
Query: 5 TVNPSLFRSESLGHYYDYNYYPD 27
T++P++ SES G YDYN + D
Sbjct: 160 TLDPTIDGSESSGEDYDYNKWND 182
>At1g69240 alpha/beta hydrolase like protein
Length = 444
Score = 26.2 bits (56), Expect = 2.8
Identities = 10/19 (52%), Positives = 13/19 (67%)
Query: 22 YNYYPDQNYTMMEKRQLFL 40
+N PD NY +ME+ LFL
Sbjct: 300 FNQQPDSNYDLMEQVHLFL 318
>At1g04730 hypothetical protein
Length = 954
Score = 25.4 bits (54), Expect = 4.8
Identities = 16/56 (28%), Positives = 26/56 (45%), Gaps = 8/56 (14%)
Query: 27 DQNYTMMEK-----RQLFLRSYQFCQKKSFTERIKGSLIRAKKVLWLRLWSSSAGG 77
DQN T+ + +L++ Y SFTE + + +LWL+ W +S G
Sbjct: 217 DQNETISAETSVMHEKLWVDKYS---PSSFTELLSDEQTNREVLLWLKQWDASVFG 269
>At5g64190 putative protein
Length = 502
Score = 25.0 bits (53), Expect = 6.2
Identities = 27/89 (30%), Positives = 42/89 (46%), Gaps = 19/89 (21%)
Query: 30 YTMMEKRQLFLRSYQFCQKKSFTERIKGSLIRA-----KKVLWLRLWSSSAGG------- 77
+++M R F Y + SFT R G +I A + V+W ++ + GG
Sbjct: 414 FSIMSPRSWFKDRYARAYR-SFTRR--GGVIFAGDEYGESVVW-KIGKGALGGTMEWEIK 469
Query: 78 -FRKLVFFPRFRCTFYYRRRR--FSRLLN 103
F L ++P TFY+ RR F++LLN
Sbjct: 470 GFIWLTYWPNKYKTFYHETRRLEFTQLLN 498
>At5g07930 putative protein
Length = 282
Score = 25.0 bits (53), Expect = 6.2
Identities = 15/55 (27%), Positives = 27/55 (48%), Gaps = 2/55 (3%)
Query: 4 VTVNPSLFRS-ESLGHYYDYNYYPDQNYTMMEKRQLF-LRSYQFCQKKSFTERIK 56
+ NP+++ S + L Y N + R++F RSY+ C+K ++ IK
Sbjct: 70 INPNPNVYESTQELPQRYSPNPSQEMTLPPTSSRRVFGRRSYELCEKVTWRTSIK 124
>At1g23830 unknown protein
Length = 345
Score = 25.0 bits (53), Expect = 6.2
Identities = 18/47 (38%), Positives = 25/47 (52%), Gaps = 8/47 (17%)
Query: 41 RSYQFCQKKSFTERIKGSLIRAKKVLWLRLWSSSAGGFRKLVFFPRF 87
RS + CQ R+ G LI + L++ LWS+S F L FF +F
Sbjct: 135 RSIKICQN-----RVGGCLITS---LYVLLWSTSVFLFFFLFFFLQF 173
>At5g59200 putative protein
Length = 590
Score = 24.6 bits (52), Expect = 8.1
Identities = 14/61 (22%), Positives = 26/61 (41%), Gaps = 11/61 (18%)
Query: 1 MASVTVNPSLFRSESLGHYYDYNYYPDQNYTMMEKRQLFLRSYQFCQKKSFTERIKGSLI 60
+A++T PS FR + PD N + +R+ + + C+ + I +I
Sbjct: 51 LAAITGGPSTFRRD-----------PDSNTLRLSRRKTLISVLRSCKNIAHVPSIHAKII 99
Query: 61 R 61
R
Sbjct: 100 R 100
>At5g24560 phloem-specific lectin-like protein
Length = 251
Score = 24.6 bits (52), Expect = 8.1
Identities = 12/33 (36%), Positives = 16/33 (48%), Gaps = 3/33 (9%)
Query: 21 DYNYYPDQNYTMMEKRQLFLRSYQFCQKKSFTE 53
DY Y D + + +QLFLR FC+ E
Sbjct: 50 DYRMYIDNSLSRFSNKQLFLR---FCESPLLIE 79
>At3g59850 polygalacturonase-like protein
Length = 388
Score = 24.6 bits (52), Expect = 8.1
Identities = 13/33 (39%), Positives = 21/33 (63%), Gaps = 4/33 (12%)
Query: 32 MMEKRQLFLRSYQF----CQKKSFTERIKGSLI 60
++ K + LRS F C++KS T RI+G+L+
Sbjct: 59 LVPKGRFLLRSIIFDGSKCKRKSVTFRIQGTLV 91
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.329 0.138 0.447
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,721,920
Number of Sequences: 26719
Number of extensions: 96986
Number of successful extensions: 282
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 17
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 264
Number of HSP's gapped (non-prelim): 24
length of query: 117
length of database: 11,318,596
effective HSP length: 93
effective length of query: 24
effective length of database: 8,833,729
effective search space: 212009496
effective search space used: 212009496
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 52 (24.6 bits)
Lotus: description of TM0048.5